1,461
Views
30
CrossRef citations to date
0
Altmetric
Original Articles

Separation, Identification, and Investigation of Antioxidant Ability of Plant Extract Components Using TLC, LC–MS, and TLC–DPPH

, &

References

  • Wang, C. C.; Chang, S. C.; Stephen Inbaraj, B.; Chen, B. H. Isolation of Carotenoids, Flavonoids and Polysaccharides from Lycium barbarum L. and Evaluation of Antioxidant Activity. Food Chem. 2010, 120, 184–192.
  • Erlund, I. Review of the Flavonoids Quercetin, Hesperetin, and Naringenin. Dietary Sources, Bioactivities, Bioavailability, and Epidemiology. Nutr. Res. 2004, 24, 851–874.
  • Manach, C.; Williamson, G.; Morand, C.; Scalbert, A.; Rémésy, C. Bioavailability and Bioefficacy of Polyphenols in Humans. I. Review of 97 Bioavailability Studies. Am. J. Clin. Nutr. 2005, 81, 230–242.
  • Shan, B.; Cai, Y. Z.; Sun, M.; Corke, H. Antioxidant Capacity of 26 Spice Extracts and Characterization of their Phenolic Constituents. J. Agric. Food Chem. 2005, 53, 7749–7759.
  • Kivilompolo, M.; Oburka, V.; Hyotylainen, T. Comparison of GC-MS and LC-MS Methods for the Analysis of Antioxidant Phenolic Acids in Herbs. Anal. Bioanal. Chem. 2007, 388, 881–887.
  • Vallverdú-Queralta, A.; Regueiroc, J.; Martínez-Huélamoa, M.; Rinaldi Alvarengad, J. F.; Neto Leale, L.; Lamuela-Raventosa, R. M. A Comprehensive Study on the Phenolic profile of Widely used Culinary Herbs and Spices: Rosemary, Thyme, Oregano, Cinnamon, Cumin and Bay. Food Chem. 2014, 154, 299–307.
  • Proestos, C.; Chorianopoulos, N.; Nychas, G. -J. E.; Komaitis, M. RP-HPLC Analysis of the Phenolic Compounds of Plant Extracts. Investigation of Their Antioxidant Capacity and Antimicrobial Activity. J. Agric. Food Chem. 2005, 53, 1190–1195.
  • Kähkönen, M. P.; Hopia, A. I.; Vuorela, H. J.; Rauha, J.-P.; Pihlaja, K.; Kujala, T. S.; Heinonen, M. Antioxidant Activity of Plant Extracts Containing Phenolic Compounds. J. Agric. Food Chem. 2005, 47, 3954–3962.
  • Rice-Evals, C. A.; Miller, N. J; Paganga, G. Antioxidant Properties of Phenolic Compounds. Trends Plant Sci. 1997, 2, 152–159.
  • Bravo, L.; Polyphenols: Chemistry, Dietary Sources, Metabolism, and Nutritional Significance. Nutr. Rev. 1998, 56, 317–333.
  • Havsteen, B. H.; The Biochemistry and Medical Significance of the Flavonoids. Pharmacol. Therapeut. 2002, 96, 67–202.
  • de Rijke, E.; Out, P.; Niessen, W. M.; Ariese, F.; Gooijer, C.; Brinkman, U. A Analytical Separation and Detection Methods for Flavonoids. J. Chromatogr. A 2006, 1112, 31–63.
  • March, R. R.; Miao, X.-S. A Fragmentation Study of Kaempferol using Electrospray Quadrupole Time-of-flight Mass Spectrometry at High Mass Resolution. Int. J. Mass Spectr. 2004, 231, 157–167.
  • Stobiecki, M. Application of Mass Spectrometry for Identification and Structural Studies of Flavonoid Glycosides. Phytochemistry, 2000, 54, 237–256.
  • Wagner, H.; Bladt, S. Plant Drug Analysis. A Thin Layer Chromatography Atlas. 2nd ed. Berlin: Springer, 2003.
  • Reich, E.; Schibli, A. High-Performance Thin-layer Chromatography for the Analysis of Medicinal Plants. New York: Thieme Medical Publisher, 2006.
  • Mażol, I.; Gleńsk, M.; Cisowski, W. Polyphenolic Compounds from Platycodon grandiflorum A. DC. Acta Pol. Pharm. 2004, 61, 203–208.
  • Głowniak, K.; Skalicka, K.; Ludwiczak, A.; Jop, K. Phenolic Compounds in the Flowers of Lavatera trimestris L. (Malvaceae), J. Planar Chromatogr. 2005, 18, 264–268.
  • Sajewicz, M.; Staszek, D.; Natic, M.; Waksmundzka-Hajnos, M.; Kowalska, T. TLC-MS versus TLC-LC-MS Fingerprints of Herbal Extracts. Part II Phenolic Acids and Flavonoids. J Liq. Chromatogr. Relat. Technol. 2011, 34, 864–887.
  • Males, Z.; Plazibat, M.; Vundac, B. V.; Zuntar, I.; Pilepic, K. H. Thin-layer Chromatographic Analysis of Flavonoids, Phenolic Acids, and Amino Acids in Some Croatian Hypericum taxa. J. Planar Chromatogr. 2004, 17, 280–285.
  • Sajewicz, M.; Staszek, D.; Natic, M.; Waksmundzka-Hajnos, M.; Kowalska, T. TLC-MS Versus TLC-LC/MS Fingerprints of Herbal Extracts. Part II Phenolic Acids and Flavonoids. J Liq. Chromatogr. Relat. Technol. 2011, 34, 864–887.
  • Fecka, I.; Kowalczyk, A.; Cisowski, W. Optimization of the Separation of Flavonoid Glycosides and Rosmarinic Acid from Mentha piperita on HPTLC plates. J. Planar Chromatogr. 2004, 17, 22–25.
  • Soczewiński, E.; Wójciak-Kosior, M.; Matysik, G. Analysis of Glycosides and Aglycones of Flavonoid Compounds by Double-development Thin-layer Chromatography. J. Planar Chromatogr. 2004, 17, 261–263.
  • Soczewiński, E.; Hawrył, M. A.; Hawrył, A. Retention Behavior of Some Flavonoids in 2D-TLC Systems on Cyano Bonded Polar Phases. Chromatographia 2001, 54, 789–794.
  • Cieśla, Ł.; Hajnos, M.; Staszek, D.; Wojtal, Ł.; Kowalska, T.; Waksmundzka-Hajnos, M. Validated Binary High-performance Thin-layer Chromatographic Fingerprints of Polyphenolics for Distinguishing Different Salvia Species. J. Chromatogr. Sci. 2010, 48, 421–427.
  • Sajewicz, M.; Staszek, D.; Natic, M.; Waksmundzka-Hajnos, M.; Kowalska, T. TLC-MS Versus TLC-LC-MS Fingerprints of Herbal Extracts. Part III. Application of the Reversed-phase Liquid Chromatography Systems with C18 Stationary Phase. J. Chromatogr. Sci. 2011, 49, 560–567.
  • Medic-Saric, M.; Jasprica, I.; Mornar, A.; Smolcic-Bubalo, A.; Golja, P. Quantitative Analysis of Flavonoids and Phenolic Acids in Propolis by Two-dimensional Thin Layer Chromatography. J. Planar Chromatogr. 2004, 17, 459–463.
  • Janeczko, Z.; Krzek, J.; Pisulewska, E.; Sobolewska, D.; Dąbrowska-Tylka, M.; Hubicka, U.; Podolak, I. Densitometric Determination of Genistin and Daidzin in Different Cultivars of Soy (Glycine max). J. Planar Chromatogr. 2004, 17, 32–35.
  • Wójciak-Kosior, M.; Matysik, G.; Skalska, A. Densitometric Determination of Kinetics of Hydrolysis of Flavonoid Glycosides. J. Planar Chromatogr. 2004, 17, 286–289.
  • Choma, I. M.; Jesionek, W. Effect-directed Biological Detection: Bioautography. In Instrumental Thin-Layer Chromatography, C. F. Poole ed. Amsterdam: Elsevier, 2014.
  • Grzelak, E. M.; Jesionek, W.; Majer-Dziedzic, B.; Choma, I. M. Applications of Novel Direct Bioautography Tests for Analysis of Antimicrobials: A Review. J. AOAC Int. 2013, 96, 1167–1174.
  • Choma, I. M. The Use of Thin-layer Chromatography with Direct Bioautography for Antimicrobial Analysis. LC-GC 2005, 18, 482–489.
  • Choma, I. M.; Grzelak, E. M. Bioautography Detection in Thin-layer Chromatography. J. Chromatogr. A 2011, 1218, 2684–2691.
  • Marston, A. Thin-layer Chromatography with Biological Detection in Phytochemistry. J. Chromatogr. A 2011, 1218, 2676–2683.
  • Cieśla, Ł.; Kryszeń, J.; Stochmal, A.; Oleszek, W.; Waksmundzka-Hajnos, M. Approach to Develop a Standardized TLC-DPPH• Test for Assessing Free Radical Scavenging Properties of Selected Phenolic Compounds. J. Pharm. Biomed. 2012, 70, 126–135.
  • Jesionek, W.; Choma, I. M.; Majer-Dziedzic, B.; Malinowska, I. Screening Bacterial and Radical Scavenging Properties of Chosen Plant Extracts Using Thin-layer Chromatography-direct Bioautography. J. Liq. Chromatogr. Rel. Technol. 2014, 37, 2882–2891.
  • Zampini, I. C.; Ordonez, R. M.; Isla, M. I. Autographic Assay for the Rapid Detection of Antioxidant Capacity of Liquid and Semi-solid Pharmaceutical Formulations Using ABTS•+ Immobilized by Gel Entrapment. AAPS Pharm. Sci. Technol. 2010, 11, 1159–1163.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.