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Original Articles

Determination of common triterpenoids and phytosterols in vegetable waxes by HPTLC—densitometry and HPTLC—image analysis

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References

  • Tiaz, L.; Zeiger, E. Plant Physiology; 5th ed.; Sinauer Associates, Inc.: Massachusetts, 2010; 370–400 pp.
  • Walton, T. J. Waxes, Cutin and Suberin. In Lipids, Membranes and Aspects of Photobiology, Methods in Plant Biochemistry; Harwood, J. L. Bowyer, J. R. Eds.; Academic Press: London, 1990, Vol. 4, 105–158 pp.
  • Kranjc E.; Albreht, A.; Vovk, I.; Makuc, D.; Plavec, J. Non-targeted Chromatographic Analyses of Cuticular Wax Flavonoids from Physalis alkekengi L. J. Chromatogr. A 2016, 1437, 95–106.
  • Ovesná, Z.; Vachálková, A.; Horváthová K.; Tóthová, D. Pentacyclic Triterpenoic Acids: New Chemoprotective Compounds. Neoplasma 2004, 51, 327–333.
  • Dzubak, P.; Hajduch, M.; Vydra, D.; Hustova, A.; Kvasnica, M.; Biedermann, D.; Markova, L.; Urban M.; Sarek, J. Pharmacological Activities of Natural Triterpenoids and Their Therapeutic Implications. Nat. Prod. Rep. 2006, 23, 394–411.
  • Patočka, J. Biologically Active Pentacyclic Triterpenes and Their Current Medicine Signification. J. Appl. Biomed. 2003, 1, 7–12.
  • Carr, T. P.; Ash, M. M.; Brown, A. W. Cholesterol-Lowering Phytosterols: Factors Affecting Their Use and Efficacy. Nutr. Diet. Suppl. 2010, 2, 59–72.
  • Martelanc, M.; Vovk, I.; Simonovska, B. Determination of Three Major Triterpenoids in Epicuticular Wax of Cabbage (Brassica oleracea L.) by High-Performance Liquid Chromatography with UV and Mass Spectrometric Detection. J. Chromatogr. A. 2007, 1164, 145–152.
  • Martelanc, M.; Vovk, I.; Simonovska, B. Separation and Identification of Some Common Isomeric Plant Triterpenoids by Thin-Layer Chromatography and High Performance Liquid Chromatography. J. Chromatogr. A. 2009, 1216, 6662–6670.
  • Naumoska K.; Vovk, I. Analysis of Triterpenoids and Phytosterols in Vegetables by Thin-Layer Chromatography Coupled to Tandem Mass Spectrometry. J. Chromatogr. A. 2015, 1381, 229–238.
  • Naumoska, K.; Simonovska, B.; Albreht A.; Vovk, I. TLC and TLC–MS Screening of Ursolic, Oleanolic and Betulinic Acids in Plant Extracts. J. Planar Chromatogr. 2013, 26, 125–131.
  • Mučaji P.; Nagy, M. Contribution to the TLC Separation of Ursolic and Oleanolic Acid Mixture. Acta Fac. Pharm. Univ. Comenianae. 2011, 58, 56–61.
  • Wójciak-Kosior, M. Separation and Determination of Closely Related Triterpenic Acids by High Performance Thin-Layer Chromatography after Iodine Derivatization. J. Pharmaceut. Biomed. 2007, 45, 337–340.
  • Simonovska B.; Vovk, I. TLC separation of some triterpenoids, Proceedings of the International Symposium Planar Chromatography Today, Novo mesto, Slovenia, October 4–6, 2002; Vovk, I., Medja, A., Eds.; National Institute of Chemistry: Ljubljana, 2002.
  • Adhyapak, S.; Dighe, V. A Normal Phase High Performance Thin Layer Chromatographic Determination of Two Triterpenoids Lupeol and Beta-Amyrin from Caesalpinia bonducella Linn. and Coccinia indica Wight & Arn. Int. J. Pharm. Pharm. Sci. 2014, 6, 449–453.
  • Li, T. -S.; Li, J. -T.; Li, H. -Z. Modified and Convenient Preparation of Silica Impregnated with Silver Nitrate and its Application to the Separation of Steroids and Triterpenes. J. Chromatogr. A. 1995, 715, 372–375.
  • Gawrońska-Grzywacz, M.; Krzaczek, T. Identification and Determination of Triterpenoids in Hieracium pilosella L. J. Sep. Sci. 2007, 30, 746–750.
  • Hernández-Vázquez, L.; Mangas, S.; Palazón J.; Navarro-Ocaña, A. Valuable Medicinal Plants and Resins: Commercial Phytochemicals with Bioactive Properties. Ind. Crop. Prod. 2010, 31, 476–480.
  • Hiermann, A.; Mayr, K. Dünnschicht-, säulen- und hochleistung-flüssigkeitschromatographischer auftrennung pflanzlicher sterole an alkylsilylierten kieselgelen. J. Chromatogr. 1986, 361, 417–420.
  • Wójciak-Kosior, M.; Sowa, I.; Kocjan R.; Jurgas, E. Investigation of Triterpenic Acids by TLC Method in Reversed-Phase System. Annales Universitatis Mariae Curie-Sklodowska. Sectio DDD: Pharmacia. 2011, 24, 113–120.
  • Wójciak-Kosior, M. Application of High Performance Thin-Layer Chromatography to Separation of Oleanolic, Ursolic and Betulinic Acids. J. Pre-Clin. Clin. Res. 2008, 1, 176–178.
  • Pozharitskaya, O. N.; Ivanova, S. A.; Shikov, A. N.; Makarov, V. G. Separation and Quantification of Terpenoids of Boswellia serrata Roxb. Extract by Planar Chromatography Techniques (TLC and AMD). J. Sep. Sci. 2006, 29, 2245–2250.
  • Nikiéma, J. B.; Vanhaelen-Fastré, R.; Vanhaelen, M. TLC Determination of a Triterpene Complex Isolated from Leptadenia hastata Latex. J. Planar Chromatogr. 1998, 11, 148–150.
  • Mallick, S.; Dighe, V. Detection and Estimation of Alpha-Amyrin, Beta-Sitosterol, Lupeol, and n-Triacontane in Two Medicinal Plants by High Performance Thin Layer Chromatography. Adv. Chem. 2014, Article ID 143948, <http://dx.doi.org/10.1155/2014/143948>.
  • Xu, S.; Norton, R. A.; Crumley, F. G.; Nes, W. D. Comparison of the Chromatographic Properties of Sterols, Select Additional Steroids and Triterpenoids: Gravity-Flow Column Liquid Chromatography, Thin-Layer Chromatography, Gas-Liquid Chromatography and High-Performance Liquid Chromatography. J. Chromatogr. 1988, 452, 377–398.
  • Bonfill, M.; Mangas, S.; Cusidó, R. M.; Osuna, L.; Piñol, M. T.; Palazón, J. Identification of Triterpenoid Compounds of Centella asiatica by Thin-Layer Chromatography and Mass Spectrometry. Biomed. Chromatogr. 2006, 20, 151–153.
  • Ahmad, Z.; Mehmood, S.; Ifzal, R.; Malik, A.; Afza, N.; Ashraf, M.; Jahan, E. A New Ursane-Type Triterpenoid from Salvia santolinifolia. Turk. J. Chem. 2007, 31, 495–501.
  • Annan, K.; Houghton, P. J. Two Novel Lupane Triterpenoids from Paullinia pinnata L. with Fibroblast Stimulatory Activity. J. Pharm. Pharmacol. 2010, 62, 663–668.
  • Srivastava, P.; Ajayakumar, P. V.; Shanker, K. Box-Behnken Design for Optimum Extraction of Biogenetic Chemicals from P. lanceolata with an Energy Audit (Thermal × Microwave × Acoustic): A Case Study of HPTLC Determination with Additional Specificity using On-line/Off-line Coupling with DAD/NIR/ESI-MS. Phytochem. Anal. 2014, 25, 551–560.
  • Scalarone, D.; Duursma, M. C.; Boon, J. J.; Chiantore, O. MALDI-TOF Mass Spectrometry on Cellulosic Surfaces of Fresh and Photo-Aged Di- and Triterpenoid Varnish Resins. J. Mass. Spectrom. 2005, 40, 1527–1535.
  • Dalvi, K.; Vaidya, V.; Menon, S.; Kekare, M.; Shah, W. Thin-Layer Chromatographic Determination of Alpha-Amyrin in the Bark of Mallotus philippensis lamk. J. Planar Chromatogr. 2007, 20, 279–281.
  • Badami, S.; Gupta, M. K.; Mathew, N.; Meyyanathan, S. N; Suresh, B.; Bendell, D. HPTLC Determination of Lupeol in Grewia tiliaefolia. J. Planar Chromatogr. 2002, 15, 183–186.
  • Jork, H.; Funk, W.; Fischer W.; Wimmer, H. Dünnschicht-Chromatographie: Reagenzien und Nachweismethoden, Bd. 1a, Physikalishe und chemische Nachweismethoden: Grundlagen, Reagenzien I, VCH Verlagsgesellschaft: Weinheim, 1989, 195–198 pp.
  • Jenks, M. A.; Ashworth, E. N. Plant Epicuticular Waxes: Function, Production and Genetics. In Horticultural reviews; Janick, J. Ed.; John Willey & Sons, Inc.: New York, 1999; Vol. 23, 1–68 pp.
  • Toivo, J.; Lampi, A. -M.; Aalto, S.; Piironen, V. Factors Affecting Sample Preparation in the Gas Chromatographic Determination of Plant Sterols in Whole Wheat Flour. Food Chem. 2000, 68, 239–245.
  • Toivo, J.; Phillips, K.; Lampi, A. -M.; Piironen, V. Determination of Sterols in Foods: Recovery of Free, Esterified, and Glycosidic Sterols. J. Food Compos. Anal. 2001, 14, 631–643.

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