Purification of Uridine Phosphorylase from Crude Extracts of Escherichia Coli Employing High-Speed Countercurrent Chromatography With an Aqueous Two-Phase Solvent System

References

• Ransohoff , T. C. , Murphy , M. K. and Levine , H. L. 1990 . BioPharm. , 3 ( 3 ) : 20 – 26 .
   Google Scholar
• Albertsson , P. A. 1986 . Partition of Cell Particles and Macro-molecules. , 3rd ed , New York : John Wiley & Sons .
   Google Scholar
• Walter , H. , Brooks , D. E. and Fisher , D. 1985 . Partitioning in aqueous two phase system: Theory, Methods, Uses, and Applica tions to biotechnology , Orlando , , U.S.A. : Academic Press Inc. .
   Google Scholar
• Kula , M. R. , Kroner , K. H. and Hustedt , H. 1982 . Purification of enzymes by liquid- liquid extraction . Adv. Biochem. Eng. , 24 : 73 – 118 .
   Google Scholar
• Tjerneld , F. and Johansson , G. 1990 . Aqueous two-phase systems for biotechnical use . Bioseparation , : 255 – 263 .
   PubMedGoogle Scholar
• Birkenmeier , G. , Kopperschlager , G. , Albertsson , P. A. , Johansson , G. , Tjerneld , F. , Akerlund , H. E. , Berner , S. and Wickstroem , H. 1987 . ldquo;Fractionation of proteins from human serum by countercurrent distribution.” . Journal of Biotechnology , 5 : 115 – 129 .
   Web of Science ®Google Scholar
• Ito , Y. 1986 . CRC Crit. Rev. Anal. Chem. , 17 : 65
   Web of Science ®Google Scholar
• Conway , W. D. 1990 . Countercurrent Chromatography: Apparatus, Theory and Applications , New York : VCH .
   Google Scholar
• Ito , Y. and Mandava , N. B. , eds. 1988 . Principle and instrumenta tion of Countercurrent Chromatography: Theory and Practice , 79 New York : Marcel Dekker . Ch. 3
   Google Scholar
• Knight , M. , Ito , Y. , Sandlin , J. L. and Kask , A. M. 1986 . J. Liquid Chromatography , 9 : 791
   Web of Science ®Google Scholar
• Ito , Y. , Kitazume , E. , Bhatnagar , M. and Trimble , F. 1991 . Cross-Axis synchronous flow-through coil planet centrifuge(Type XLL). I. Design of the apparatus and studies on retention of station ary phase . J. of Liq. Chromatogr. , 538 : 59 – 66 .
   Web of Science ®Google Scholar
• Ito , Y. 1991 . Cross-Axis Synchronous flow-through coil planet cen trifuge (Type XLL) II. Speculation on the hydrodynamic mechanism in stationary phase retention . J. of Liq. Chroma togr. , 538 : 67 – 80 .
   PubMed Web of Science ®Google Scholar
• Shibusawa , Y. and Ito , Y. 1991 . Protein separation with aqueous-aqueous polymer systems by two types of counter-current chromatographies . J. of Chromatogr. , 550 : 695 – 704 .
   PubMed Web of Science ®Google Scholar
• Welton , L. , Richards , C. A. and Elwell , L. P. 1989 . Nucleic Acid Res , 17 : 6741
   PubMed Web of Science ®Google Scholar
• Aldea , M. , Maples , V. F. and Kushner , S. R. 1988 . J. Mol. Biol , 200 : 427 – 438 .
   PubMed Web of Science ®Google Scholar
• Weaver , K. , Chen , D. , Walton , L. , Elwell , L. and Ray , P. July/Aug 25–28 1990 . July/Aug 25–28 , Biopharm
   Google Scholar
• Laemmli , U. K. 1970 . Nature , 227 : 680 – 685 .
   PubMed Web of Science ®Google Scholar
• Kvenitsky , T. A. 1976 . J. Biol. Chem. , 251 : 4055 – 4061 .
   PubMed Web of Science ®Google Scholar