High Performance Liquid Chromatographic (HPLC) Assay for the Determination of Chlorhexidine in Saliva Film

References

• Rose , F. L. and Swain , G. 1956 . Bisdiguanides having antibacterial activity . J. Chem. Soc. , : 4422 – 4425 .
   Google Scholar
• Hennessey , T. D. 1977 . Antibacterial properties of Hibitane . J. Clin. Periodont. , 4 : 36 – 48 .
   PubMed Web of Science ®Google Scholar
• Netuschil , L. , Reich , E. and Brecx , M. 1989 . Direct measurement of the bactericidal effect of chlorhexidine on human dental plaque . J. Clin. Periodontol. , 16 : 484 – 488 .
   PubMed Web of Science ®Google Scholar
• Wade , W. G. and Addy , M. 1989 . In vitro activity of a chlorhexidine-containing mouthwash against subgingival bacteria . J. Periodontol. , 60 : 521 – 525 .
   Web of Science ®Google Scholar
• Addy , M. , Jenkins , S. and Newcombe , R. 1991 . The effect of some chlorhexidine-containing mouthrinses on salivary bacterial counts . J. Clin. Periodontol. , 18 : 90 – 93 .
   Web of Science ®Google Scholar
• Davies , R. M. , B⊘rglumJensen , S. , Rindom Schi⊘tt , C. and Löe , H. 1970 . The effect of topical application of chlorhexidine on bacterial colonization of the teeth and gingiva . J. Periodont Res. , 5 : 96 – 101 .
   PubMedGoogle Scholar
• Bonesvoll , P. , Lökken , P. , Rölla , G. and Paus , P. N. 1974 . Retention of chlorhexidine in the human oral cavity after mouth rinses . Archs. Oral Biol. , 19 : 209 – 212 .
   PubMed Web of Science ®Google Scholar
• Bonesvoll , P. 1978 . Retention and plaque-inhibiting effect in man of chlorhexidine after multiple mouth rinses and retention and release of chlorhexidine after toothbrushing with a chlorhexidine gel . Arch. Oral Biol. , 23 : 295 – 300 .
   PubMed Web of Science ®Google Scholar
• Bonesvoll , P. and Olsen , I. 1974 . Influence of teeth, plaque and dentures on the retention of chlorhexidine in the human oral cavity . J. Clin. Periodontol. , 1 : 214 – 221 .
   PubMedGoogle Scholar
• Flötra , L. , Gjermo , P. , Rölla , G. and Waerhaug , J. 1971 . Side effects of chlorhexidine mouth washes . Scand. J. Dent Res. , 79 : 119 – 125 .
   PubMedGoogle Scholar
• Eriksen , H. M. , Nordb⊘ , H. , Kantanen , H. and Ellingsen , J. E. 1985 . Chemical plaque control and extrinsic tooth discoloration. A review of the possible mechanisms . J. Clin. Periodontol. , 12 : 345 – 350 .
   PubMed Web of Science ®Google Scholar
• Addy , M , Rawle , L. , Handley , R. , Newman , H. N. and Coventry , J. F. 1982 . The development and in vitro evaluation of acrylic strips and dialysis tubing for local drug delivery . J. Periodontol. , 53 : 693 – 699 .
   PubMed Web of Science ®Google Scholar
• Friedman , M. and Golomb , G. 1982 . New sustained release dosage form of chlorhexidine for dental use . J. Periodont. Res. , 17 : 323 – 328 .
   PubMed Web of Science ®Google Scholar
• Mirth , D. B. , Bartkiewicz , A. , Shern , R. J. and Little , W. A. 1989 . Development and in vitro evaluation of an intra-oral controlled-release delivery system for chlorhexidine . J. Dent. Res. , 68 : 1285 – 1288 .
   PubMed Web of Science ®Google Scholar
• Collins , L. M.C. and Dawes , C. 1987 . The surface area of the adult human mouth and thickness of the salivary film covering the teeth and oral mucosa . J. Dent. Res. , 66 : 1300 – 1302 .
   PubMed Web of Science ®Google Scholar
• Hattingh , J. and Ho , E. 1980 . The concentration of proteins in human gingival crevicular fluid . J. Periodont. Res. , 15 : 90 – 95 .
   Google Scholar
• Britt , M. R. and Pohlod , D. J. 1986 . Serum and crevicular fluid concentrations after a single oral dose of metronidazole . J. Periodontol. , 57 : 104 – 107 .
   PubMed Web of Science ®Google Scholar
• Higashi , K. , Seike , M. , Mitani , Y. , Morisaki , K. , Hayashi , S. , Kitamura , M. , Fujimoto , N. , Kimura , S. , Ebisu , S. and Okada , H. 1989 . Concentration of ofloxacin in human gingival crevicular fluid after oral administration of Tarivid . J. Periodont. Res. , 24 : 409 – 411 .
   PubMed Web of Science ®Google Scholar
• Stolze , K. 1992 . Concentration of metronidazole in periodontal pockets after application of a metronidazole 25% dental gel . J. Clin. Periodontol. , 19 : 698 – 701 .
   Google Scholar
• Bauer , M. , Degude , C. and Mailhe , L. 1984 . Simultaneous determination of chlorhexidine, tetracaine and their degradation products by ion-pair liquid chromatography . J. Chromatogr. , 315 : 457 – 464 .
   PubMed Web of Science ®Google Scholar
• Richard , A. , Elbaz , M. and Andermann , G. 1984 . Determination of 4-chloroaniline and chlorhexidine digluconate by ion-pair reversed-phase high-performance liquid chromatography . J. Chromatogr. , 298 : 356 – 359 .
   Web of Science ®Google Scholar
• Huston , C. E. , Wainwright , P. and Cooke , M. 1982 . High-performance liquid chromatographic method for the determination of chlorhexidine . J. Chromatogr. , 237 : 457 – 464 .
   Google Scholar
• Gaffney , M. H. and Cooke , M. 1984 . Improved method for the determination of chlorhexidine in urine . J. Chromatogr. , 306 : 303 – 313 .
   PubMed Web of Science ®Google Scholar
• Brougham , L. R. , Cheung , H. and Pittman , K. A. 1986 . Sensitive high-performance liquid chromatographic method for the determination of chlorhexidine in human serum and urine . J. Chromatogr. , 383 : 365 – 373 .
   PubMed Web of Science ®Google Scholar
• Wainwright , P. and Cooke , M. 1986 . Direct determination of chlorhexidine in urine by high-performance liquid chromatography . Analyst , 111 : 1343 – 1344 .
   PubMed Web of Science ®Google Scholar
• Lentner , C , ed. 1981 . “ Units of Measurement, Body fluids, Composition of the body, Nutrition ” . In Geigy Scientific Tables , Vol. 1 , 114 – 122 . Basle : Ciba-Geigy Ltd. .
   Google Scholar
• Medlicott , N. J. , Jones , D. S. , Tucker , I. G. and Holborow , D. 1992 . Preliminary release studies of chlorhexidine (base and diacetate) from poly(α-caprolactone) films prepared by solvent evaporation . Int. J. Pharm. , 84 : 85 – 89 .
   Web of Science ®Google Scholar
• Stanley , A. , Wilson , M. and Newman , H. N. 1989 . The in vitro effects of chlorhexidine on subgingival plaque bacteria . J. Clin. Periodontol. , 16 : 259 – 264 .
   PubMed Web of Science ®Google Scholar
• Dawes , C and Weatherell , J. A. 1990 . Kinetics of fluoride in the oral fluids . J. Dent Res. , 69 : 638 – 644 . special issue
   PubMedGoogle Scholar