Genotoxic potential of bee venom (Apis Mellifera) on human peripheral blood lymphocytes in vitro using single cell gel electrophoresis assay

References

• Billingham , M. E. , Morley , J. , Hanson , J. M. , Shipolini , R. A. and Vernon , C. A. 2001 . Letter: An anti-inflammatory peptide from bee venom . Nature , 245 ( 5421 ) : 163 – 164 .
   Google Scholar
• Hider , R. C. 1988 . Honeybee venom: a rich source of pharmacologically active peptides . Endeavour , 12 ( 2 ) : 60 – 65 .
   PubMed Web of Science ®Google Scholar
• Varanda , E. A. and Tavares , D. C. 1998 . Radioprotection: Mechanism and radioprotective agents including honey bee venom . Venom. Anim. Toxins , 4 ( 1 ) : 5 – 21 .
   Google Scholar
• Varanda , E. A. , Monti , R. and Tavares , D. C. 1999 . Inhibitory effect of propolis and bee venom on the mutagenicity of some direct- and indirect-acting mutagens . Teratog. Carcinog. Mutagen. , 19 ( 6 ) : 403 – 413 .
   PubMedGoogle Scholar
• Sumikura , H. , Andersen , O. K. , Drewes , A. M. and Arendt-Nielsen , L. A. 2003 . Comparison of hyperalgesia and neurogenic inflammation induced by melittin and capsaicin in humans . Neurosci. Lett. , 337 ( 3 ) : 147 – 150 .
   PubMed Web of Science ®Google Scholar
• Nam , K. W. , Je , K. H. , Lee , J. H. , Han , H. J. , Lee , H. J. , Kang , S. K. and Mar , W. 2003 . Inhibition of COX-2 activity and proinflammatory cytokines (TNF-alpha and IL-1beta) production by water-soluble sub-fractionated parts from bee (Apis mellifera) venom . Arch. Pharm. Res. , 26 ( 5 ) : 383 – 388 .
   PubMed Web of Science ®Google Scholar
• Kim , H. W. , Kwon , Y. B. , Ham , T. W. , Roh , D. H. , Yoon , S. Y. , Lee , H. J. , Han , H. J. , Yang , I. S. , Beitz , A. J. and Lee , J. H. 2003 . Acupoint stimulation using bee venom attenuates formalin-induced pain behavior and spinal cord fos expression in rats . J. Vet. Med. Sci. , 65 ( 3 ) : 349 – 355 .
   PubMed Web of Science ®Google Scholar
• Orsolic , N. , Sver , L. , Verstovsek , S. , Terzic , S. and Basic , I. 2003 . Inhibition of mammary carcinoma cell proliferation in vitro and tumor growth in vivo by bee venom . Toxicon , 41 ( 7 ) : 861 – 870 .
   PubMed Web of Science ®Google Scholar
• Habermann , E. 1972 . Bee and wasp venoms . Science , 177 ( 46 ) : 314 – 322 .
   PubMed Web of Science ®Google Scholar
• Dotimas , E. M. , Hamid , K. R. , Hider , R. C. and Ragnarsson , U. 1987 . Isolation and structure analysis of bee venom mast cell degranulating peptide . Biochim. Biophys. Acta , 911 ( 3 ) : 285 – 293 .
   PubMedGoogle Scholar
• Lariviere , W. R. and Melzack , R. 1996 . The bee venom test: a new tonic-pain test . Pain , 66 ( 2–3 ) : 271 – 277 .
   PubMed Web of Science ®Google Scholar
• Neuman , W. and Habermann , E. 1954 . Characterization of the substances of the bee venom . Naunyn Schmiedebergs Arch. Exp. Pathol. Pharmakol. , 222 ( 4 ) : 267 – 287 .
   PubMedGoogle Scholar
• Vogel , H. 1981 . Incorporation of melittin into phosphatidylcholine bilayers. Study of binding and conformational changes . FEBS Lett. , 134 ( 1 ) : 37 – 42 .
   PubMed Web of Science ®Google Scholar
• Gevod , V. S. and Birdi , K. S. 1984 . Melittin and the 8–26 fragment. Differences in ionophoric properties as measured by monolayer method . Biophys. J. , 45 ( 6 ) : 1079 – 1083 .
   PubMed Web of Science ®Google Scholar
• Habermann , E. 1971 . “ Chemistry, pharmacology, and toxicology of bee, wasp and hornet venoms ” . In Venomous Animals and Their Venoms, vol. 3 , Edited by: Bucherl , W. and Buckley , E. E. 61 – 93 . New York : Academic Press .
   Google Scholar
• Fletcher , J. E. and Jiang , M. S. 1993 . Possible mechanisms of action of cobra snake venom cardiotoxins and bee venom melittin . Toxicon , 31 : 669 – 695 .
   PubMed Web of Science ®Google Scholar
• Liu , X. , Chen , D. , Xie , L. and Zhang , R. 2002 . Effect of honey bee venom on proliferation of K1735M2 mouse melanoma cells in-vitro and growth of murine B16 melanomas in-vivo . J. Pharm. Pharmacol. , 54 ( 8 ) : 1083 – 1089 .
   PubMed Web of Science ®Google Scholar
• Jang , M. H. , Shin , M. C. , Lim , S. , Han , S. M. , Park , H. J. and Shin , I. 2003 . Bee venom induces apoptosis and inhibits expression of cyclooxygenase-2 mRNA in human lung cancer cell line NCI-H1299 . J. Pharmacol. Sci. , 91 ( 2 ) : 95 – 104 .
   PubMed Web of Science ®Google Scholar
• Yin , C. S. , Lee , H. J. , Hong , S. J. , Chung , J. H. and Koh , H. G. 2005 . Microarray analysis of gene expression in chondrosarcoma cells treated with bee venom . Toxicon , 45 ( 1 ) : 81 – 91 .
   PubMed Web of Science ®Google Scholar
• Hu , H. , Chen , D. , Li , Y. and Zhang , X. 2006 . Effect of polypeptides in bee venom on growth inhibition and apoptosis induction of the human hepatoma cell line SMMC-7721 in-vitro and Balb/c nude mice in-vivo . J. Pharm. Pharmacol. , 58 ( 1 ) : 83 – 89 .
   PubMed Web of Science ®Google Scholar
• Moon , D. O. , Park , S. Y. , Heo , M. S. , Kim , K. C. , Park , C. and Ko , W. S. 2006 . Key regulators in bee venom-induced apoptosis are Bcl-2 and caspase-3 in human leukemic U937 cells through downregulation of ERK and Akt . Int. Immunopharmacol. , 6 ( 12 ) : 1796 – 1807 .
   PubMed Web of Science ®Google Scholar
• Han , S. , Lee , K. , Yeo , J. , Kweon , H. , Woo , S. , Lee , M. , Baek , H. , Kim , S. and Park , K. 2007 . Effect of honey bee venom on microglial cells nitric oxide and tumor necrosis factor-alpha production stimulated by LPS . J. Ethnopharmacol. , 111 ( 1 ) : 176 – 181 .
   PubMed Web of Science ®Google Scholar
• Lee , Y. J. , Kang , S. J. , Kim , B. M. , Kim , Y. J. , Woo , H. D. and Chung , H. W. 2007 . Cytotoxicity of honeybee (Apis mellifera) venom in normal human lymphocytes and HL-60 cells . Chem. Biol. Interact. , 169 ( 3 ) : 189 – 197 .
   PubMed Web of Science ®Google Scholar
• Singh , N. P. , McCoy , M. T. , Tice , R. R. and Schneider , L. L. 1988 . A simple technique for quantitation of low levels of DNA damage in individual cells . Exp. Cell. Res. , 175 : 184 – 191 .
   PubMed Web of Science ®Google Scholar
• Collins , A. R. 2004 . The comet assay for DNA damage and repair: principles, applications, and limitations . Mol. Biotechnol. , 26 ( 3 ) : 249 – 261 .
   PubMed Web of Science ®Google Scholar
• Dixon , D. R. , Pruski , A. M. , Dixon , L. R. J. and Jha , A. N. 2002 . Marine invertebrate eco-genotoxicology: a methodological overview . Mutagenesis , 17 : 495 – 507 .
   PubMed Web of Science ®Google Scholar
• Fairbairn , D. W. , Olive , P. L. and O'Neill , K. L. 1995 . The Comet Assay: A comprehensive review . Mutat. Res. , 339 : 37 – 59 .
   PubMed Web of Science ®Google Scholar
• Lee , R. F. and Steinert , S. 2003 . Use of the single cell gel electrophoresis/Comet Assay for detecting DNA damage in aquatic (marine and freshwater) animals . Mutat. Res. , 544 : 43 – 64 .
   PubMed Web of Science ®Google Scholar
• Collins , A. , Dusinska , M. , Franklin , M. , Somorovska , M. , Petrovska , H. , Duthie , S. , Fillion , L. , Panayiotidis , M. , Raslová , K. and Vaughan , N. 1997 . Comet Assay in human biomonitoring studies: reliability, validation, and applications . Environ. Mol. Mutagen. , 30 : 139 – 146 .
   PubMed Web of Science ®Google Scholar
• Kumaravel , T. S. , Vilhar , B. , Faux , S. P. and Jha , A. N. 2007 . Comet Assay measurements: a perspective . Cell. Biol. Toxicol. , (Epub ahead of print) DOI: 10.1007/s10565-007-9043-9
   PubMed Web of Science ®Google Scholar
• Tice , R. R. , Yager , J. W. , Andrews , P. and Crecelius , E. 1997 . Effect of hepatic methyl donor status on urinary excretion and DNA damage in B6C3F1 mice treated with sodium arsenite . Mutat. Res. , 386 ( 3 ) : 315 – 334 .
   PubMed Web of Science ®Google Scholar
• Gajski , G. , Ravli[cgrave] , S. , Capuder , Ž. and Garaj-Vrhovac , V. 2007 . Use of sensitive methods for detection of DNA damage on human lymphocytes exposed to P,P′-DDT: Comet assay and new criteria for scoring micronucleus test . J. Environ. Sci. Health Pt. B , 42 ( 6 ) : 607 – 613 .
   PubMed Web of Science ®Google Scholar
• Gajski , G. and Garaj-Vrhovac , V. 2008 . Application of cytogenetic endpoints and comet assay on human lymphocytes treated with atorvastatin in vitro . J. Environ. Sci. Health Pt. A , 43 ( 1 ) : 78 – 85 .
   Google Scholar
• Olive , P. , Banáth , J. P. and Durand , R. E. 1990 . Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells using the comet assay . Radiat. Res. , 122 : 86 – 94 .
   PubMed Web of Science ®Google Scholar
• Hellman , B. , Vaghef , H. and Boström , B. 1995 . The concepts of tail moment and tail inertia in the single cell gel electrophoresis assay . Mutat. Res. , 336 : 123 – 131 .
   PubMed Web of Science ®Google Scholar
• Olive , P. L. 1999 . DNA damage and repair in individual cells: applications of the comet assay in radiobiology . Int. J. Radiat. Biol. , 75 : 395 – 405 .
   PubMed Web of Science ®Google Scholar
• Piperakis , S. M. , Visvardis , E. E. and Tassiou , A. M. 1999 . Comet assay for nuclear DNA damage . Meth. Enzymol. , 300 : 184 – 194 .
   PubMed Web of Science ®Google Scholar