103
Views
5
CrossRef citations to date
0
Altmetric
Original Articles

Laser microdissection on Norway spruce bark tissue: A suitable protocol for subsequent real-time reverse transcription–polymerase chain reaction (RT-PCR) analysis

, , , &
Pages 92-98 | Received 22 Mar 2010, Accepted 23 Jun 2010, Published online: 01 Jun 2011

References

  • Agustí , J , Merelo , P , Cercós , M , Tadeo , F R and Talón , M . 2009 . Comparative transcriptional survey between laser-microdissected cells from laminar abscission zone and petiolar cortical tissue during ethylene-promoted abscission in citrus leaves . BMC Plant Biol , 9 : 127
  • Angeles , G , Berrio-Sierra , J , Joseleau , J P , Lorimier , P , Lefebvre , A and Ruel , K . 2006 . Preparative laser capture microdissection and single-pot cell wall material preparation: A novel method for tissue-specific analysis . Planta , 224 : 228 – 232 .
  • Balestrini , R and Bonfante , P . 2008 . Laser microdissection (LM): Application to plant materials . Plant Biosyst , 142 : 331 – 336 .
  • Brandt , S P . 2005 . Microgenomics: Gene expression analysis at the tissue-specific and single-cell levels . J Exp Bot , 56 : 495 – 505 .
  • Casson , S , Spencer , M , Walker , K and Lindsey , K . 2005 . Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis . Plant J , 42 : 111 – 123 .
  • Chandran , D , Inada , N , Hather , G , Kleindt , C K and Wildermuth , M C . 2010 . Laser microdissection of Arabidopsis cells at the powdery mildew infection site reveals site-specific processes and regulators . Proc Natl Acad Sci USA , 107 : 460 – 465 .
  • Corpas , F J , Fernandez-Ocana , A , Carreras , A , Valderrama , R , Luque , F Esteban , F J . 2006 . The expression of different superoxide dismutase forms is cell-type dependent in olive (Olea europaea L.) leaves . Plant Cell Physiol , 47 : 984 – 994 .
  • Day , R C , Grossniklaus , U and Macknight , R C . 2005 . Be more specific! Laser microdissection of plant cells . Trends Plant Sci , 10 : 397 – 406 .
  • Day , R C , Herridge , R P , Ambrose , B A and Macknight , R C . 2008 . Transcriptome analysis of proliferating Arabidopsis endosperm reveals biological implications for the control of syncytial division, cytokinin signalling, and gene expression regulation . Plant Physiol , 148 : 1964 – 1984 .
  • Dembinsky , D , Woll , K , Saleem , M , Liu , Y , Fu , Y Borsuk , L A . 2007 . Transcriptomic and proteomic analyses of pericycle cells of the maize primary root . Plant Physiol , 145 : 575 – 588 .
  • Gelmini , S , Poggesi , M , Pinzani , P , Mannurita , S C , Cianchi , F Valanzano , R . 2006 . Distribution of Tankyrase-1 mRNA expression in colon cancer and its prospective correlation with progression stage . Oncol Rep , 16 : 1261 – 1266 .
  • Gomez , S K and Harrison , M J . 2009 . Laser microdissection and its application to analyze gene expression in arbuscular mycorrhizal symbiosis . Pest Manag Sci , 65 : 504 – 511 .
  • Hietala , A M , Kvaalen , H , Schmidt , A , Johnk , N , Solheim , H and Fossdal , C G . 2004 . Temporal and spatial profiles of chitinase expression by Norway spruce in response to bark colonization by Heterobasidion annosum . Appl Environ Microbiol , 70 : 3948 – 3953 .
  • Hobo , T , Suwabe , K , Aya , K , Suzuki , G , Yano , K Ishimizu , T . 2008 . Various spatiotemporal expression profiles of anther-expressed genes in rice . Plant Cell Physiol , 49 : 1417 – 1428 .
  • Huang , L E , Luzzi , V , Ehrig , T , Holtschlag , V and Watson , M A . 2002 . Optimized tissue processing and staining for laser capture microdissection and nucleic acid retrieval . Meth Enzymol , 356 : 49 – 62 .
  • Inada , N and Wildermuth , M C . 2005 . Novel tissue preparation method and cell-specific marker for laser microdissection of Arabidopsis mature leaf . Planta , 221 : 9 – 16 .
  • Johnk , N , Hietala , A M , Fossdal , C G , Collinge , D B and Newman , M . 2005 . Defense-related genes expressed in Norway spruce roots after infection with the root rot pathogen Ceratobasidium bicorne (anamorph: Rhizoctonia sp.) . Tree Physiol , 25 : 1533 – 1543 .
  • Johnsen , Ø , Fossdal , C G , Nagy , N , Mølmann , J , Dæhlen , O and Skrøppa , T . 2005 . Climatic adaptation in Picea abies progenies is affected by the temperature during zygotic embryogenesis and seed maturation . Plant Cell Environ , 28 : 1090 – 1102 .
  • Kehr , J . 2003 . Single cell technology . Curr Op Plant Biol , 6 : 617 – 621 .
  • Kerk , N M , Ceserani , T , Tausta , S L , Sussex , I M and Nelson , T M . 2003 . Laser capture microdissection of cells from plant tissues . Plant Physiol , 132 : 27 – 35 .
  • Klink , V P , Hosseini , P , Matsye , P D , Alkharouf , N W and Matthews , B F . 2010 . Syncytium gene expression in Glycine max ([PI 88788]) roots undergoing a resistant reaction to the parasitic nematode Heterodera glycines . Plant Physiol Biochem , 48 : 176 – 193 .
  • Li , S H , Schneider , B and Gershenzon , J . 2007 . Microchemical analysis of laser-microdissected stone cells of Norway spruce by cryogenic nuclear magnetic resonance spectroscopy . Planta , 225 : 771 – 779 .
  • Luchi , N , Ma , R , Capretti , P and Bonello , P . 2005 . Systemic induction of traumatic resin ducts and resin flow in Austrian pine by wounding and inoculation with Sphaeropsis sapinea and Diplodia scrobiculata . Planta , 221 : 75 – 84 .
  • Morrogh , M , Olvera , N , Bogomolniy , F , Borgen , P I and King , T A . 2007 . Tissue preparation for laser capture microdissection and RNA extraction from fresh frozen breast tissue . BioTechniques , 43 : 41 – 48 .
  • Nakashima , J , Chen , F , Jackson , L , Shadle , G and Dixon , R A . 2008 . Multi-site genetic modification of monolignol biosynthesis in alfalfa (Medicago sativa): Effects on lignin composition in specific cell types . New Phytol , 179 : 738 – 750 .
  • Pinzani , P , Lind , K , Malentacchi , F , Nesi , G , Salvianti , F Villari , D . 2008 . Prostate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase-quantitative polymerase chain reaction and immuno-quantitative polymerase chain reaction . Hum Pathol , 39 : 1474 – 1482 .
  • Pinzani , P , Orlando , C and Pazzagli , M . 2006 . Laser-assisted microdissection for real-time PCR sample preparation . Mol Asp Med , 27 : 140 – 159 .
  • Ruijter , J M , Ramakers , C , Hoogaars , W , Bakker , O , van den Hoff , M JB Karlen , Y . 2009 . Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data . Nucleic Acids Res , 37 : e45
  • Scanlon , M J , Ohtsu , K , Timmermans , M C and Schnable , P S . 2009 . Laser microdissection-mediated isolation and in vitro transcriptional amplification of plant RNA . Curr Protoc Mol Biol , (Unit 25A.3): 1–15. DOI: 10.1002/0471142727.mb25a03s87
  • Schad , M , Mungur , R , Fiehn , O and Kehr , J . 2005 . Metabolic profiling of laser microdissected vascular bundles of Arabidopsis thaliana . Plant Meth , 1 : 1 – 10 .
  • Tang , W , Coughlan , S , Crane , E , Beatty , M and Duvick , J . 2006 . The application of laser microdissection to in planta gene expression profiling of the maize anthracnose stalk rot fungus Colletotrichum graminicola . Mol Plant Micr Inter , 19 : 1240 – 1250 .
  • Yakovlev , I , Fossdal , C G , Johnsen , Ø , Junttila , O and Skrøppa , T . 2006 . Analysis of gene expression during bud burst initiation in Norway spruce via ESTs from subtracted cDNA libraries . Tree Gen Genomes , 2 : 39 – 52 .
  • Zhang , X , Madi , S , Borsuk , L , Nettleton , D , Elshire , R J Buckner , B . 2007 . Laser microdissection of narrow sheath mutant maize uncovers novel gene expression in the shoot apical meristem . PLoS Genet , 3 : e101

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.