Genetic variability of relict Rhododendron ferrugineum L. populations in the Northern Apennines with some inferences for a conservation strategy

References

• Alessandrini , A and Bonafede , F . 1996 . “ Atlante della Flora Protetta della Regione Emilia-Romagna, Regione Emilia-Romagna ” . Bologna
   Google Scholar
• Ansaldi , M , Cassettari , A and Adami , M . 2003 . Rhododendron ferrugineum L. (Ericaceae), specie nuova per la flora apuana . Atti Soc Tosc Sci Nat Mem Ser B , 110 : 189 – 191 .
   Google Scholar
• Antonovics , J , Newman , T J and Best , B J . 2001 . “ Spatially explicit studies on the ecology and genetics of population margins ” . In Integrating ecology and evolution in a spatial context , Edited by: Silvertown , J and Antonovics , J . 97 – 116 . Oxford : Blackwell Science .
   Google Scholar
• Aparajitaa , S and Routa , G R . 2009 . Genetic differentiation of Albizia lebbeck (L.) Benth. populations estimated by RAPD and ISSR markers . Plant Biosyst , 143 : 361 – 368 .
   Web of Science ®Google Scholar
• Austerlitz , F and Garnier-Géré , P . 2003 . Modelling the impact of colonisation on genetic diversity and differentiation of forest trees: interaction of life cycle, pollen flow and seed long-distance dispersal . Heredity , 90 : 282 – 290 .
   PubMed Web of Science ®Google Scholar
• Bianco , J and Bulard , C . 1974 . Etude de la germination des graines de Rhododendron ferrugineum L. et de Tofieldea calyculata L. Wahlnb . Trav Sci Parc Nat Vanoise , V : 121 – 130 .
   Google Scholar
• Brown , J H . 1984 . On the relationship between abundance and distribution of species . Am Nat , 124 : 255 – 279 .
   Web of Science ®Google Scholar
• De Mattia , F , Grassi , F , Imazio , S and Labra , M . 2008 . Chloroplast and nuclear DNA markers to characterize cultivated and spontaneous Ribes . Plant Biosyst , 142 : 204 – 212 .
   Web of Science ®Google Scholar
• Diaci , J , Rozenbergar , D and Boncina , A . 2009 . Stand dynamics of Dinaric old-growth forest in Slovenia: are indirect human influences relevant . Plant Biosyst , 144 : 194 – 201 .
   Web of Science ®Google Scholar
• Eckert , C G , Samis , K E and Lougheed , S C . 2008 . Genetic variation across species' geographical ranges: the central-marginal hypothesis and beyond . Mol Ecol , 17 : 1170 – 1188 .
   PubMed Web of Science ®Google Scholar
• Ellstrand , N C and Elam , D R . 1993 . Population genetic consequences of small population size: implications for plant conservation . Annu Rev Ecol Syst , 24 : 217 – 242 .
   Google Scholar
• El Mousadik , A and Petit , R J . 1996 . High level of genetic differentiation for allelic richness among populations of the argan tree [Argania spinosa (L.) Skeels] endemic to Morocco . Theor Appl Genet , 92 : 832 – 839 .
   PubMed Web of Science ®Google Scholar
• Escaravage , N , Pornon , A , Doche , B and Till-Bottraud , I . 1997 . Breeding system in alpine species: Rhododendron ferrugineum L . (Ericaceae) in the French northern Alps. Can J Bot , 75 : 736 – 743 .
   Google Scholar
• Escaravage , N , Questiau , S , Pornon , A , Doche , B and Taberlet , P . 1998 . Clonal diversity in a Rhododendron ferrugineum L. (Ericaceae) population inferred from AFLP markers . Mol Ecol , 7 : 975 – 982 .
   Web of Science ®Google Scholar
• Escaravage , N and Wagner , J . 2004 . Pollination effectiveness and pollen dispersal in a Rhododendron ferrugineum (Ericaceae) population . Plant Biol , 6 : 606 – 615 .
   PubMed Web of Science ®Google Scholar
• Evanno , G , Regnaut , S and Goudet , J . 2005 . Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study . Mol Ecol , 14 : 2611 – 2620 .
   PubMed Web of Science ®Google Scholar
• Excoffier , L , Laval , G and Schneider , S . 2005 . Arlequin ver. 3.0: an integrated software package for population genetics data analysis . Evol Bioinform Online , 1 : 47 – 50 .
   PubMed Web of Science ®Google Scholar
• Falush , D , Stephens , M and Pritchard , J K . 2003 . Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies . Genetics , 164 : 1567 – 1687 .
   PubMed Web of Science ®Google Scholar
• Ferrari , C and Piccoli , F . 1997 . The ericaceous dwarf shrublands above the Northern Apennine timberline (Italy) . Phytocoenologia , 27 : 53 – 76 .
   Web of Science ®Google Scholar
• Ferrarini , E . 1973 . Rhododendron ferrugineum L. in fitocenosi relitte dell'Appennino settentrionale . Giornale Botanico Italiano , 107 : 143 – 156 .
   Google Scholar
• Ferrarini , E . 1974 . Altre cenosi e stazioni relitte reperite sull' Appennino settentrionale (ad Antennaria carpatica, a Rhododendron ferrugineum, a Salix herbacea, a Saussurea discolor) . Webbia , 29 : 105 – 112 .
   Google Scholar
• Ferrarini , E . 1979 . Note floristiche sull'Appennino settentrionale (dal Passo della Cisa al Passo delle Radici) . Webbia , 33 : 235 – 267 .
   Google Scholar
• Fluch , S , Berenyi , M , Hristoforoglu , K , Kopecky , D and Burg , K . “ Submitted. Development of a microsatellite marker system for the Alpine species Rhododendron ferrugineum and Rhododendron hirsutum ” . In Mol Ecol Resour
   Google Scholar
• Goudet , J . 2001 . “ FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3) ” . Available: http://www.unil.ch/izea/softwares/fstat.htm. Accessed Feb 2011 28
   Google Scholar
• Grabherr , G . 2009 . Biodiversity in the high ranges of the Alps: ethnobotanical and climate change perspectives . Global Environ Change , 19 : 167 – 172 .
   Web of Science ®Google Scholar
• Guarino , C , Santoro , S , De Simone , L , Cipriani , G and Testolin , R . 2008 . Differentiation in DNA fingerprinting among species of the genus Acer L . in Campania (Italy). Plant Biosyst , 142 : 454 – 461 .
   Web of Science ®Google Scholar
• Guerrant , E O , Havens , K and Maunder , M . 2004 . “ Ex situ plant conservation: supporting species survival in the wild ” . Covelo , USA : Island Press .
   Google Scholar
• Hampe , A and Petit , R J . 2005 . Conserving biodiversity under climate change: the rear edge matters . Ecol Lett , 8 : 461 – 467 .
   PubMed Web of Science ®Google Scholar
• Hedrick , P W . 2000 . “ Genetics of populations ” . Massachusetts , , USA : Jones & Bartlett, Sudbury .
   Google Scholar
• Hengeveld , R and Haeck , J . 1982 . The distribution of abundance. I. Measurements . J Biogeogr , 9 : 303 – 316 .
   Web of Science ®Google Scholar
• Jian , S and Shi , S . 2009 . Genetic variation in Heritiera littoralis (Malvaceae) from east and south Asia revealed by AFLP markers . Plant Biosyst , 143 : S56 – S62 .
   Web of Science ®Google Scholar
• Körner , C . 2003 . Alpine plant life: functional plant ecology of high mountains ecosystems. , 2nd ed , Berlin–Heidelberg : Springer .
   Google Scholar
• Langella , O . 2007 . “ Populations 1.2.30: population genetic software (individuals or populations distances, phylogenetic trees), France ” . Available: http://bioinformatics.org/∼tryphon/populations/. Accessed Feb 2011 28
   Google Scholar
• Lenormand , T . 2002 . Gene flow and the limits to natural selection . Trends Ecol Evol , 17 : 183 – 189 .
   Web of Science ®Google Scholar
• Linington , S . 2000 . “ The millenium seed bank project ” . In Biological collections and biodiversity , Edited by: Rushton , B S , Hackney , P and Tyrie , C R . Vol. 3 , Linnean Society of London Special Publication . UK: Westbury Publishing. 121–125
   Google Scholar
• Lowe , J J , Davite , C , Moreno , D and Maggi , R . 1994 . Holocene pollen stratigraphy and human interference in the woodlands of the northern Apennines, Italy . Holocene , 4 : 153 – 164 .
   Google Scholar
• Lönn , M and Prentice , H C . 2002 . Gene diversity and demographic turnover in central and peripheral populations of the perennial herb, Gypsophila fastigiata . Oikos , 99 : 489 – 498 .
   Web of Science ®Google Scholar
• Manel , S , Berthoud , F , Bellemain , M , Gaudeul , M , Luikart , G Swenson , J E . 2007 . A new individual-based spatial approach for identifying genetic discontinuities in natural populations . Mol Ecol , 16 : 2031 – 2043 .
   PubMed Web of Science ®Google Scholar
• Nathan , R , Shmida , A and Fragman , O . 1996 . “ Peripherality and regional rarity are positively correlated: quantitative evidence from the upper Galilea Flora (North Israel) ” . In Preservation of our world in the wake of change (Vol.VI A/B ISEEQS Pub: 561–564) , Edited by: Steinberger , Y . Jerusalem , , Israel : Linnean Society of London Special Publication .
   Google Scholar
• Nei , M , Tajima , F and Tateno , Y . 1983 . Accuracy of estimated phylogenetic trees from molecular data. II. Gene frequency data . J Mol Evol , 19 : 153 – 170 .
   PubMed Web of Science ®Google Scholar
• Ohlemüller , R , Anderson , B J and Araújo , M B . 2008 . The coincidence of climatic and species rarity: high risk to small-range species from climate change . Biol Lett , 4 : 568 – 572 .
   PubMed Web of Science ®Google Scholar
• Peakall , R and Smouse , P E . 2006 . GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research . Mol Ecol Notes , 6 : 288 – 295 .
   Web of Science ®Google Scholar
• Pornon , A and Escaravage , N . 1999 . Genotypic structure in clonal Rhododendron ferrugineum L. (Ericaceae) populations: origin and maintenance . Plant Ecol , 141 : 145 – 150 .
   Web of Science ®Google Scholar
• Pornon , A , Escaravage , N , Thomas , P and Taberlet , P . 2000 . Dynamics of genotypic structure in clonal Rhododendron ferrugineum (Ericaceae) populations . Mol Ecol , 9 : 1099 – 1111 .
   PubMed Web of Science ®Google Scholar
• Pornon , A , Escaravage , N , Till-Bottraud , I and Doche , B . 1997 . Variation of reproductive traits in Rhododendron ferrugineum (Ericaceae) populations along a successional gradient . Plant Ecol , 130 : 1 – 11 .
   Web of Science ®Google Scholar
• Powell , W , Morgante , M , Andre , C , Hanafey , M , Vogel , J , Tingley , S and Rafalski , A . 1996 . The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis . Mol Breed , 2 : 225 – 238 .
   Web of Science ®Google Scholar
• Pritchard , J K , Stephens , M and Donnelly , P . 2000 . Inference of population structure using multilocus genotype data . Genetics , 155 : 945 – 959 .
   PubMed Web of Science ®Google Scholar
• Pritchard , J K , Wen , W and Falush , D . 2007 . “ STRUCTURE ver. 2.2. University of Chicago, Chicago ” . Available: http://pritch.bsd.uchicago.edu/. Accessed Feb 2011 28
   Google Scholar
• Rabinowitz , D . 1981 . “ Seven forms of rarity ” . In The biological aspects of rare plant conservation , Edited by: Synge , H . 205 – 217 . New York : John Wiley & Sons Ltd .
   Google Scholar
• Rossi , G and Orrico , D . 1996 . Pedo-anthracological studies on the evolution of the upper treeline in the Tuscan-Emilian Apennines: preliminary data . Arch Geobot , 2 : 173 – 174 .
   Google Scholar
• Rossi , G. , Parolo , G. and Ulian , T. 2009 . Human trampling as a threat factor for the conservation of peripheral plant populations . Plant Biosyst , 143 : 104 – 113 .
   Web of Science ®Google Scholar
• Rousset , F . 1997 . Genetic differentiation and estimation of gene flow from F-statistics under isolation by distance . Genetics , 145 : 219 – 1228 .
   Google Scholar
• Scialabba , A , Agrimonti , C , Abbate , G M and Marmiroli , N . 2008 . Assessment of genetic variation in Sicilian Helichrysum (Asteraceae) and implication to germplasm conservation . Plant Biosyst , 142 : 287 – 297 .
   Web of Science ®Google Scholar
• Thuiller , W , Lavorel , S , Arau’ jo , M B , Sykes , M T and Prentice , I C . 2005 . Climate change threats to plant diversity in Europe . Proc Natl Acad Sci USA , 102 : 8245 – 8250 .
   PubMed Web of Science ®Google Scholar
• Tranquillini , W . 1979 . “ Physiological ecology of the Alpine Timberline. Tree existence at high altitudes with special reference to the European Alps ” . New York and Berlin : Springer Verlag . Ecological Studies, 31
   Google Scholar
• Van Oosterhout , C , Hutchinson , W F , Wills , D PM and Shipley , P . 2004 . MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data . Mol Ecol Notes , 4 : 535 – 538 .
   Web of Science ®Google Scholar
• Weir , B S and Cockerham , C C . 1984 . Estimating F-statistics for the analysis of population structure . Evolution , 38 : 1358 – 1370 .
   PubMed Web of Science ®Google Scholar
• Wolf , P G , Doche , B , Gielly , L and Taberlet , P . 2004 . Genetic Structure of Rhododendron ferrugineum at a Wide Range of Spatial Scales . J Hered , 95 : 301 – 308 .
   PubMed Web of Science ®Google Scholar
• Wright , S . 1943 . Isolation by distance . Genetics , 28 : 114 – 138 .
   PubMedGoogle Scholar
• Yeh , F C , Yang , R C and Boyle , T . 1999 . “ POPGENE version 1.31: Microsoft window-based freeware for population genetic analysis ” . Alberta , Canada : University of Alberta and Centre for International Forestry Research .
   Google Scholar