Expression of PACT and EIF2C2, Implicated in RNAi and MicroRNA pathways, in various human cell lines

References

• Bartel , DP . 2004 . MicroRNAs: genomics, biogenesis, mechanism, and function . Cell , 116 : 281 – 297 .
   PubMed Web of Science ®Google Scholar
• Billy , E , Brondani , V , Zhang , H , Muller , U and Filipowicz , W . 2001 . Specific interference with gene expression induced by long, double‐stranded RNA in mouse embryonal teratocarcinoma cell lines . Proc Natl Acad Sci USA , 98 : 14428 – 14433 .
   PubMed Web of Science ®Google Scholar
• Carmell , MA , Xuan , Z , Zhang , MQ and Hannon , GJ . 2002 . The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis . Genes Dev , 16 : 2733 – 2742 .
   PubMed Web of Science ®Google Scholar
• Cerutti , L , Mian , N and Bateman , A . 2000 . Domains in gene silencing and cell differentiation proteins: the novel PAZ domain and redefinition of the Piwi domain . Trends Biochem Sci , 25 : 481 – 482 .
   PubMed Web of Science ®Google Scholar
• Dignam , JD , Lebovitz , RM and Roeder , RG . 1983 . Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei . Nucleic Acids Res , 11 : 1475 – 1489 .
   PubMed Web of Science ®Google Scholar
• Doench , JG , Petersen , CP and Sharp , PA . 2003 . siRNAs can function as miRNAs . Genes Dev , 17 : 438 – 442 .
   PubMed Web of Science ®Google Scholar
• Doi , N , Zenno , S , Ueda , R , Ohki‐Hamazaki , H , Ui‐Tei , K and Saigo , K . 2003 . Shortinterfering‐RNA‐mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors . Curr Biol , 13 : 41 – 46 .
   PubMed Web of Science ®Google Scholar
• Dykxhoorn , DM , Novina , CD and Sharp , PA . 2003 . Killing the messenger: short RNAs that silence gene expression . Nat Rev Mol Cell Biol , 4 : 457 – 467 .
   PubMed Web of Science ®Google Scholar
• Ekwall , K . 2004 . The RITS complex‐A direct link between small RNA and heterochromatin . Mol Cell , 13 : 304 – 305 .
   PubMed Web of Science ®Google Scholar
• Elbashir , SM , Harborth , J , Lendeckel , W , Yalcin , A , Weber , K and Tuschl , T . 2001 . Duplexes of 21‐nucleotide RNAs mediate RNA interference in cultured mammalian cells . Nature , 411 : 494 – 498 .
   PubMed Web of Science ®Google Scholar
• Fire , A , Xu , S , Montgomery , MK , Kostas , SA , Driver , SE and Mello , CC . 1998 . Potent and specific genetic interference by double‐stranded RNA in Caenorhabditis elegans . Nature , 391 : 806 – 811 .
   PubMed Web of Science ®Google Scholar
• Fukagawa , T , Nogami , M , Yoshikawa , M , Ikeno , M , Okazaki , T , Takami , Y , Nakayama , T and Oshimura , M . 2004 . Dicer is essential for formation of the heterochromatin structure in vertebrate cells . Nat Cell Biol , 6 : 784 – 791 .
   PubMed Web of Science ®Google Scholar
• Gupta , V and Patel , RC . 2002 . Proapoptotic protein PACT is expressed at high levels in colonic epithelial cells in mice . Am J Physiol Gastrointest Liver Physiol , 283 : G801 – 808 .
   Web of Science ®Google Scholar
• Hammond , SM , Bernstein , E , Beach , D and Hannon , GJ . 2000 . An RNA‐directed nuclease mediates post‐transcriptional gene silencing in Drosophila cells . Nature , 404 : 293 – 296 .
   PubMed Web of Science ®Google Scholar
• Hammond , SM , Boettcher , S , Caudy , AA , Kobayashi , R and Hannon , GJ . 2001 . Argonaute2, a link between genetic and biochemical analyses of RNAi . Science , 293 : 1146 – 1150 .
   PubMed Web of Science ®Google Scholar
• Hannon , GJ . 2002 . RNA interference . Nature , 418 : 244 – 251 .
   PubMed Web of Science ®Google Scholar
• Hutvagner , G , McLachlan , J , Pasquinelli , AE , Balint , E , Tuschl , T and Zamore , PD . 2001 . A cellular function for the RNA‐interference enzyme dicer in the maturation of the let‐7 small temporal RNA . Science , 293 : 834 – 838 .
   PubMed Web of Science ®Google Scholar
• Hutvagner , G and Zamore , PD . 2002 . A microRNA in a multiple‐turnover RNAi enzyme complex . Science , 297 : 2056 – 2060 .
   PubMed Web of Science ®Google Scholar
• Ishizuka , A , Siomi , MC and Siomi , H . 2002 . A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins . Genes Dev , 16 : 2497 – 2508 .
   PubMed Web of Science ®Google Scholar
• Lee , RC , Feinbaum , RL and Ambros , V . 1993 . The C. elegans heterochronic gene lin‐4 encodes small RNAs with antisense complementarity to lin‐14 . Cell , 75 : 843 – 854 .
   PubMed Web of Science ®Google Scholar
• Lingel , A , Simon , B , Izaurralde , E and Sattler , M . 2003 . Structure and nucleic‐acid binding of the Drosophila Argonaute 2 PAZ domain . Nature , 426 : 465 – 469 .
   PubMed Web of Science ®Google Scholar
• Liu , Q , Rand , TA , Kalidas , S , Du , F , Kim , HE , Smith , DP and Wang , X . 2003 . R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway . Science , 301 : 1921 – 1925 .
   PubMedGoogle Scholar
• Martinez , J , Patkaniowska , A , Urlaub , H , Luhrmann , R and Tuschl , T . 2002 . Singlestranded antisense siRNAs guide target RNA cleavage in RNAi . Cell , 110 : 563 – 574 .
   PubMed Web of Science ®Google Scholar
• Mourelatos , Z , Dostie , J , Paushkin , S , Sharma , A , Charroux , B , Abel , L , Rappsilber , J , Mann , M and Dreyfuss , G . 2002 . miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs . Genes Dev , 16 : 720 – 728 .
   PubMed Web of Science ®Google Scholar
• Okamura , K , Ishizuka , A , Siomi , H and Siomi , MC . 2004 . Distinct roles for Argonaute proteins in small RNA‐directed RNA cleavage pathways . Genes Dev , 18 : 1655 – 1666 .
   PubMed Web of Science ®Google Scholar
• Patel , RC and Sen , GC . 1998 . PACT, a protein activator of the interferon‐induced protein kinase, PKR . EMBO J , 17 : 4379 – 4390 .
   PubMed Web of Science ®Google Scholar
• Reinhart , BJ , Slack , FJ , Basson , M , Pasquinelli , AE , Bettinger , JC , Rougvie , AE , Horvitz , HR and Ruvkun , G . 2000 . The 21‐nucleotide let‐7 RNA regulates developmental timing in Caenorhabditis elegans . Nature , 403 : 901 – 906 .
   PubMed Web of Science ®Google Scholar
• Sambrook , J , Fritsch , EF and Maniatis , T . 1989 . Molecular Cloning: a Laboratory Manual , Cold Spring Harbor : Cold Spring Harbor Laboratory .
   Google Scholar
• Sasaki , T , Shiohama , A , Minoshima , S and Shimizu , N . 2003 . Identification of eight members of the Argonaute family in the human genome small star, filled . Genomics , 82 : 323 – 330 .
   PubMed Web of Science ®Google Scholar
• Saxena , S , Jonsson , ZO and Dutta , A . 2003 . Small RNAs with imperfect match to endogenous mRNA repress translation. Implications for off‐target activity of small inhibitory RNA in mammalian cells . J Biol Chem , 278 : 44312 – 44319 .
   PubMed Web of Science ®Google Scholar
• Schwarz , DS and Zamore , PD . 2002 . Why do miRNAs live in the miRNP? . Genes Dev , 16 : 1025 – 1031 .
   PubMed Web of Science ®Google Scholar
• Song , JJ , Liu , J , Tolia , NH , Schneiderman , J , Smith , SK , Martienssen , RA , Hannon , GJ and Joshua‐Tor , L . 2003 . The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes . Nat Struct Biol , 10 : 1026 – 1032 .
   PubMedGoogle Scholar
• Stark , GR , Kerr , IM , Williams , BR , Silverman , RH and Schreiber , RD . 1998 . How cells respond to interferons . Annu Rev Biochem , 67 : 227 – 264 .
   PubMed Web of Science ®Google Scholar
• Tabara , H , Sarkissian , M , Kelly , WG , Fleenor , J , Grishok , A , Timmons , L , Fire , A and Mello , CC . 1999 . The rde‐1 gene, RNA interference, and transposon silencing in C. elegans . Cell , 99 : 123 – 132 .
   PubMed Web of Science ®Google Scholar
• Tabara , H , Yigit , E , Siomi , H and Mello , CC . 2002 . The dsRNA binding protein RDE‐4 interacts with RDE‐1, DCR‐1, and a DExH‐box helicase to direct RNAi in C. elegans . Cell , 109 : 861 – 871 .
   PubMed Web of Science ®Google Scholar
• Tang , G , Reinhart , BJ , Bartel , DP and Zamore , PD . 2003 . A biochemical framework for RNA silencing in plants . Genes Dev , 17 : 49 – 63 .
   PubMed Web of Science ®Google Scholar
• Yang , S , Tutton , S , Pierce , E and Yoon , K . 2001 . Specific double‐stranded RNA interference in undifferentiated mouse embryonic stem cells . Mol Cell Biol , 21 : 7807 – 7816 .
   PubMed Web of Science ®Google Scholar
• Zeng , Y and Cullen , BR . 2002 . RNA interference in human cells is restricted to the cytoplasm . RNA , 8 : 855 – 860 .
   PubMed Web of Science ®Google Scholar
• Zeng , Y , Wagner , EJ and Cullen , BR . 2002 . Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells . Mol Cell , 9 : 1327 – 1333 .
   PubMed Web of Science ®Google Scholar
• To whom correspondence should be addressed. Tel: 1–434–924–1227, Fax: 1–434–924–5069 E‐mail: [email protected]
   Google Scholar