Multiple-site fragment deletion, insertion and substitution mutagenesis by modified overlap extension PCR

References

• Higuchi R, Krummel B, Saiki RK. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res. 1988;16:7351–7367.
   PubMed Web of Science ®Google Scholar
• Warrens AN, Jones MD, Lechler RI. Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest. Gene. 1997;186(1):29–35.
   PubMed Web of Science ®Google Scholar
• Chiu J, March PE, Lee R, et al. Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h. Nucleic Acids Res. 2004;32(21):e174.
   PubMed Web of Science ®Google Scholar
• Allemandou F, Nussberger J, Brunner HR, et al. Rapid site-directed mutagenesis using two-PCR-generated DNA fragments reproducing the plasmid template. J Biomed Biotechnol. 2003;2003(3):202–207.
   PubMedGoogle Scholar
• Tyagi R, Lai R, Duggleby RG. A new approach to ‘megaprimer’ polymerase chain reaction mutagenesis without an intermediate gel purification step. BMC Biotechnol. 2004;4:2.
   PubMed Web of Science ®Google Scholar
• Kirsch RD, Joly E. An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes. Nucleic Acids Res. 1998;26(7):1848–1850.
   PubMed Web of Science ®Google Scholar
• Kegler-Ebo DM, Docktor CM, DiMaio D. Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes. Nucleic Acids Res. 1994;22(9):1593–1599.
   PubMed Web of Science ®Google Scholar
• Zoller MJ, Smith M. Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA. Nucleic Acids Res. 1982;10(20):6487–6500.
   PubMed Web of Science ®Google Scholar
• Edelheit O, Hanukoglu A, Hanukoglu I. Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure–function studies. BMC Biotechnol. 2009;9(1):61.
   Google Scholar
• Mikaelian I, Sergeant A. A general and fast method to generate multiple site directed mutations. Nucleic Acids Res. 1992;20(2):376.
   PubMed Web of Science ®Google Scholar
• Jensen PH, Weilguny D. Combination primer polymerase chain reaction for multi-site mutagenesis of close proximity sites. J Biomol Tech. 2005;16(4):336–340.
   PubMedGoogle Scholar
• Young L, Dong Q. TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening. Nucleic Acids Res. 2003;31(3):e11. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC149223/
   Google Scholar
• Kim YG, Maas S. Multiple site mutagenesis with high targeting efficiency in one cloning step. Biotechniques. 2000;28(2):196–198.
   PubMed Web of Science ®Google Scholar
• Wang W, Malcolm BA. Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis. Biotechniques. 1999;26(4):680–682.
   PubMed Web of Science ®Google Scholar
• Liu H, Naismith JH. An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol. BMC Biotechnol. 2008;8:91. Available from: http://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-8-91
   Google Scholar
• Liang X, Peng L, Li K, et al. A method for multi-site-directed mutagenesis based on homologous recombination. Anal Biochem. 2012;427(1):99–101.
   PubMed Web of Science ®Google Scholar
• Guo W, Sun S, Xie B, et al. T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis. Biotechnol Biotechnol Equip. 2016;30(4):721–725.
   Web of Science ®Google Scholar
• Trehan A, Kielbus M, Czapinski J, et al. REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering. Sci Rep. 2016;6:19121.
   Google Scholar
• Mitchell LA, Cai Y, Taylor M, et al. Multichange isothermal mutagenesis: a new strategy for multiple site-directed mutations in plasmid DNA. ACS Synth Biol. 2013;2(8):473–477.
   PubMed Web of Science ®Google Scholar
• Ho SN, Hunt HD, Horton RM, et al. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 1989;77(1):51–59.
   PubMed Web of Science ®Google Scholar
• Bryksin AV, Matsumura I. Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids. Biotechniques. 2010;48(6):463–465.
   PubMed Web of Science ®Google Scholar
• Urban A, Neukirchen S, Jaeger KE. A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR. Nucleic Acids Res. 1997;25(11):2227–2228.
   PubMed Web of Science ®Google Scholar
• An Y, Ji J, Wu W, et al. A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR. Appl Microbiol Biotechnol. 2005;68(6):774–778.
   PubMed Web of Science ®Google Scholar
• Lee J, Shin MK, Ryu DK, et al. Insertion and deletion mutagenesis by overlap extension PCR. Methods Mol Biol. 2010;634:137–146.
   PubMedGoogle Scholar
• Waneskog M, Bjerling P. Multi-fragment site-directed mutagenic overlap extension polymerase chain reaction as a competitive alternative to the enzymatic assembly method. Anal Biochem. 2014;444:32–37.
   PubMed Web of Science ®Google Scholar
• Williams EM, Copp JN, Ackerley DF. Site-saturation mutagenesis by overlap extension PCR. Methods Mol Biol. 2014;1179:83–101.
   PubMedGoogle Scholar
• Ito W, Ishiguro H, Kurosawa Y. A general method for introducing a series of mutations into cloned DNA using the polymerase chain reaction. Gene. 1991;102(1):67–70.
   PubMed Web of Science ®Google Scholar
• Jung V, Pestka SB, Pestka S. Efficient cloning of PCR generated DNA containing terminal restriction endonuclease recognition sites. Nucleic Acids Res. 1990;18(20):6156.
   PubMed Web of Science ®Google Scholar
• Zheng L, Baumann U, Reymond JL. An efficient one-step site-directed and site-saturation mutagenesis protocol. Nucleic Acids Res. 2004;32(14):e115.
   Google Scholar