1,475
Views
7
CrossRef citations to date
0
Altmetric
Article; Pharmaceutical Biotechnology

Soluble expression and purification of heparinase I in Escherichia coli using a hexahistidine-tagged small ubiquitin-like modifier as a fusion partner

, , , , , , & show all
Pages 1040-1045 | Received 24 Dec 2016, Accepted 11 Jul 2017, Published online: 19 Jul 2017

References

  • Laurent P, Dussarat GV, Bonal J, et al. Low molecular weight heparins: a guide to their optimum use in pregnancy. Drug. 2002;62(3):463–477.
  • Godavarti R, Sasisekharan R. Heparinase I from Flavobacterium heparinum. Role of positive charge in enzymatic activity. J Biol Chem. 1998;273:248–255.
  • Ernst S, Venkataraman G, Winkler S, et al. Expression in Escherichia coli, purification and characterization of heparinase I from Flavobacterium heparinum. J Biol Chem. 1996;315(2):589–597.
  • Chen Y, Xing X-H, Ye F, et al. Production of MBP–HepA fusion protein in recombinant Escherichia coli by optimization of culture medium. Biochem Eng J. 2007;34:114–121.
  • Kane JF. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr Opin Biotechnol. 1995;6:494–500.
  • Terpe K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. 2003;60:523–533.
  • Bernstein H, Yang VC, Cooney CL, et al. Immobilized heparin lyase system for blood deheparinization. Methods Enzymol. 1988;137:515–529.
  • Sasisekharan R, Bulmer M, Moremen KW, et al. Cloning and expression of heparinase I gene from Flavobacterium heparinum. Proc Natl Acad Sci USA. 1993;90(8):3660–3664.
  • Shpigel E, Goldlust A, Efroni G, et al. Immobilization of recombinant heparinase I fused to cellulose-binding domain. Biotechnol Bioeng. 1999;65(1):17–23.
  • Davis GD, Elisee C, Newham DM, et al. New fusion protein systems designed to give soluble expression in Escherichia coli. Biotechnol Bioeng. 1999;65(4):382–388.
  • Wang C, Castro AF, Wilkes DM, et al. Expression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product: comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding protein. Biochem J. 1999;338(Pt 1):77–81.
  • Esposito D, Chatterjee DK. Enhancement of soluble protein expression through the use of fusion tags. Curr Opin Biotechnol. 2006;17(4):353–358.
  • Jia B, Jeon CO. High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives. Open Biol. 2016;6(8):160196. DOI: 10.1098/rsob.160196
  • Marblestone JG, Edavettal SC, Lim Y, et al. Comparison of SUMO fusion technology with traditional gene fusion systems: enhanced expression and solubility with SUMO. Protein Sci. 2006;15(1):182–189.
  • Butt TR, Edavettal SC, Hall JP, et al. SUMO fusion technology for difficult-to-express proteins. Protein Expr Purif. 2005;43(1):1–9.
  • Liu D, Shrive Z, Godavarti R, et al. The calcium-binding sites of heparinase I from Flavobacterium heparinum are essential for enzymatic activity. J Biol Chem. 1999;274(7):4089–4095.
  • Ma X, Wang Z, Li S, et al. Effect of CaCl2 as activity stabilizer on purification of heparinase I from Flavobacterium heparinum. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;843(2):209–215.
  • Shriver Z, Liu D, Hu Y, et al. Biochemical investigations and mapping of the calcium-binding sites of heparinase I from Flavobacterium heparinum. J Biol Chem. 1999;274(7):4082–4088.