MCT cloning: a seamless cloning strategy for inserting DNA fragments

References

• Tillett D, Neilan BA. Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites. Nucleic Acids Res. 1999;27:e26–e28.
   PubMed Web of Science ®Google Scholar
• Liu Z. Hetero-stagger cloning: efficient and rapid cloning of PCR products. Nucleic Acids Res. 1996;24:2458–2459.
   PubMed Web of Science ®Google Scholar
• Klock HE, Koesema EJ, Knuth MW, et al. Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts. Proteins. 2008;71:982–994.
   PubMed Web of Science ®Google Scholar
• Klock HE, Lesley SA. The Polymerase Incomplete Primer Extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis. Methods Mol Biol. 2009;498:91–103.
   PubMedGoogle Scholar
• Rashtchian A, Buchman GW, Schuster DM, et al. Uracil DNA glycosylase-mediated cloning of polymerase chain reaction-amplified DNA: application to genomic and cDNA cloning. Anal Biochem. 1992;206:91–97.
   PubMed Web of Science ®Google Scholar
• Nisson PE, Rashtchian A, Watkins PC. Rapid and efficient cloning of Alu-PCR products using uracil DNA glycosylase. PCR Methods Appl. 1991;1:120–123.
   PubMedGoogle Scholar
• Aslanidis C, de Jong PJ. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 1990;18:6069–6074.
   PubMed Web of Science ®Google Scholar
• van den Ent F, Lowe J. RF cloning: a restriction-free method for inserting target genes into plasmids. J Biochem Biophys Methods. 2006;67:67–74.
   PubMedGoogle Scholar
• Unger T, Jacobovitch Y, Dantes A,et al. Applications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression. J Struct Biol. 2010;172:34–44.
   PubMed Web of Science ®Google Scholar
• Braman J, Papworth C, Greener A. Site-directed mutagenesis using double-stranded plasmid DNA templates. In: Trower MK, editor. In vitro mutagenesis protocols. Totowa (NJ): Humana Press; 1996. p. 31–44.
   Google Scholar
• Bauer JC (SD, CA), Wright DA (Cambridge, MA), Braman JC (Carlsbad, CA), et al., inventors; Stratagene (La Jolla, CA), Children's Medical Center Corp. (Longwood, MA), assignee. Circular site-directed mutagenesis. United States Patent 6391548. 2002.
   Google Scholar
• Quan J, Tian J. Circular polymerase extension cloning of complex gene libraries and pathways. PLoS One. 2009 [cited 2009 Jan 16];4:e6441. [6 p.] DOI:10.1371/journal.pone.0006441
   PubMed Web of Science ®Google Scholar
• Quan J, Tian J. Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries. Nat Protoc. 2011;6:242–251.
   PubMed Web of Science ®Google Scholar
• Ulrich A, Andersen KR, Schwartz TU. Exponential megapriming PCR (EMP) cloning–seamless DNA insertion into any target plasmid without sequence constraints. PLoS One. 2012 [cited 2012 Dec 31];7:e53360. [9 p.] DOI:10.1371/journal.pone.0053360
   PubMed Web of Science ®Google Scholar
• Liu C-J, Jiang H, Wu L, et al OEPR cloning: an efficient and seamless cloning strategy for large- and multi-fragments. Sci Rep-UK. 2017 [cited 2017 Mar 16];7:44648 [p7] DOI:10.1038/srep44648
   PubMed Web of Science ®Google Scholar
• Ho SN, Hunt HD, Horton RM,et al. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 1989;77:51–59.
   PubMed Web of Science ®Google Scholar
• Spiliotis M. Inverse fusion PCR cloning. PloS One. 2012 [cited 2017 Mar 16];7:e35407. [8 p.] DOI:10.1371/journal.pone.0035407
   PubMed Web of Science ®Google Scholar
• Karimi M, Inzé D, Depicker A. GATEWAY™ vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci. 2002;7:193–195.
   PubMed Web of Science ®Google Scholar
• Zhu B, Cai G, Hall EO, et al. In-fusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations. BioTechniques. 2007;43:354–359.
   PubMed Web of Science ®Google Scholar
• Zhang Y, Werling U, Edelmann W. SLiCE: a novel bacterial cell extract-based DNA cloning method. Nucleic Acids Res. 2012 [cited 2012 Apr 1];40:e55 [p 10] DOI:10.1093/nar/gkr1288
   PubMed Web of Science ®Google Scholar
• Okegawa Y, Motohashi K. A simple and ultra-low cost homemade seamless ligation cloning extract (SLiCE) as an alternative to a commercially available seamless DNA cloning kit. Biochem Biophys Rep. 2015;4:148–151.
   PubMedGoogle Scholar
• Motohashi K. Evaluation of the efficiency and utility of recombinant enzyme-free seamless DNA cloning methods. Biochem Biophys Rep. 2017;9:310–315.
   PubMedGoogle Scholar
• Kostylev M, Otwell AE, Richardson RE, et al. Cloning should be simple: Escherichia coli DH5α-mediated assembly of multiple DNA fragments with short end homologies. PloS One. 2015 [cited 2015 Sep 5];10:e0137466 [p 15] DOI:10.1371/journal.pone.0137466
   PubMed Web of Science ®Google Scholar
• Motohashi K. A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis. BMC Biotechnol. 2015 [cited 2015 June 3];15(1):47. [p 9]DOI:10.1186/s12896-015-0162-8
   PubMedGoogle Scholar
• Liang J, Liu Z, Low XZ, et al. Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method. Nucleic Acids Res. 2017 [cited 2017 June 20];45:e94.[p 11] DOI:10.1093/nar/gkx132
   PubMed Web of Science ®Google Scholar
• Zeng F, Hao Z, Li P, et al. A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends. BMC Biotechnol. 2017 [cited 2017 Mar 1];17:32.[p 7] DOI:10.1186/s12896-017-0346-5
   PubMed Web of Science ®Google Scholar