References
- Imbeaud S, Graudens E, Boulanger V, et al. Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Nucleic Acids Res. 2005;33(6):1–9. doi: 10.1093/nar/gni054.
- Bustin SA. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol. 2002;29(1):23–39. doi: 10.1677/JME.0.0290023.
- Pérez-Novo CA, Claeys C, Speleman F, et al. Impact of RNA quality on reference gene expression stability. Biotechniques. 2005;39(1):52–56. doi: 10.2144/05391BM05.
- Jahn CE, Charkowski AO, Willis DK. Evaluation of isolation methods and RNA integrity for bacterial RNA quantitation. J Microbiol Methods. 2008;75(2):318–324. doi: 10.1016/j.mimet.2008.07.004.
- Bustin SA. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J Biomol Tech. 2004;15(3):155–166.
- Mueller O, Hahnenberger K, Dittmann M, et al. A microfluidic system for high-speed reproducible DNA sizing and quantitation. Electrophoresis. 2000;21(1):128–134. doi: 10.1002/(SICI)1522-2683(20000101)21:1<128::AID-ELPS128>3.0.CO;2-M.
- Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001;25(4):402–408. doi: 10.1006/METH.2001.1262.
- Applied Biosystems. Qubit RNA IQ assay: a fast and easy fluorometric RNA quality assessment. Thermo Fish. 2017; Accessed: Jan. 27, 2023. [Online]:[1 p.]. Available from: https://www.thermofisher.com/document-connect/document-connect.html?url=https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0012655_GeneJET_Plasmid_Miniprep_UG.pdf%0Ahttps://www.thermofisher.com/document-connect/document-connect.html?url=https
- Shedlovskiy D, Shcherbik N, Pestov DG. One-step hot formamide extraction of RNA from Saccharomyces cerevisiae. RNA Biol. 2017;14(12):1722–1726. doi: 10.1080/15476286.2017.1345417.
- Pfaffl MW, Tichopad A, Prgomet C, et al. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol Lett. 2004;26(6):509–515. [Online]. Available from: http://www.wzw.tum.de/gene-quantification/bestkeeper.html doi: 10.1023/b:bile.0000019559.84305.47.
- In Bustin SA, editor. A-Z of Quantitative PCR. La Jolla, CA, USA: IUL Press; 2004; pp. 90–91
- Lin-Chao S, Bremer H. Effect of the bacterial growth rate on replication control of plasmid pBR322 in Escherichia coli. Mol Gen Genet. 1986;203(1):143–149. doi: 10.1007/BF00330395.
- Stead MB, Agrawal A, Bowden KE, et al. RNAsnapTM: a rapid, quantitative and inexpensive, method for isolating total RNA from bacteria. Nucleic Acids Res. 2012;40(20):e156–e156. doi: 10.1093/nar/gks680.
- Oñate-Sánchez L, Verdonk JC. Citrate-citric acid RNA isolation (CiAR) for fast, low-cost, and reliable RNA extraction from multiple plant species and tissues. Curr Protoc. 2021;1(12). doi: 10.1002/cpz1.298.
- Earl CC, Smith MT, Lease RA, et al. Polyvinylsulfonic acid: a low-cost rnase inhibitor for enhanced RNA preservation and cell-free protein translation. Bioengineered. 2018;9(1):90–97. doi: 10.1080/21655979.2017.1313648.
- Fregel R, González A, Cabrera VM. Improved ethanol precipitation of DNA. Electrophoresis. 2010;31(8):1350–1352. doi: 10.1002/elps.200900721.
- Dilworth DD, McCarrey JR. Single-step elimination of contaminating DNA prior to reverse transcriptase PCR. PCR Methods Appl. 1992;1(4):279–282. doi: 10.1101/gr.1.4.279.
- Quale J, Bratu S, Gupta J, et al. Interplay of efflux system, ampC, and oprD expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates. Antimicrob Agents Chemother. 2006;50(5):1633–1641. doi: 10.1128/AAC.50.5.1633-1641.2006.
- Nadin-Davis S, Mezl VA. Optimization of the ethanol precipitation of RNA from formamide containing solutions. Prep Biochem. 1982;12(1):49–56. doi: 10.1080/00327488208065549.
- Metal Chelates. https://www.dojindo.eu.com/images/Product Photo/Chelate_Table_of_Stability_Constants.pdf. (accessed Jun. 13, 2023).
- Rhee SS, Burke DH. Tris(2-carboxyethyl)phosphine stabilization of RNA: comparison with dithiothreitol for use with nucleic acid and thiophosphoryl chemistry. Anal Biochem. 2004;325(1):137–143. doi: 10.1016/j.ab.2003.10.019.
- Chomczynski P. Solubilization in formamide protects RNA from degradation. Nucleic Acids Res. 1992;20(14):3791–3792. doi: 10.1093/NAR/20.14.3791.
- Gaillard C, Strauss F. Ethanol precipitation of DNA with linear polyacrylamide as carrier. Nucleic Acids Res. 1990;18(2):378. doi: 10.1093/nar/18.2.378.
- QIAxcel®—Pure Excellence. https://www.qiagen.com/∼/media/nextq/resource library/brochures/qiaxcel-pure-excellence.ashx. (accessed Jun. 13, 2023).