Assessment of heavy metal contamination using real-time PCR analysis of mussel metallothionein mt10 and mt20 expression: a validation along the Tunisian coast

References

• Amiard JC, Amiard-Triquet C, Barka S, Pellerin J, Rainbowd P-S. Metallothioneins in aquatic invertebrates: Their role in metal detoxification and their use as biomarkers. Aquatic Toxicology 2006; 76: 160–202
   PubMed Web of Science ®Google Scholar
• Amiard JC, Pineau A, Boiteau H-L, Metayer C, Amiard-Triquet C. Application de la spectrométrie d'absorption atomique Zeeman aux dosages de huit éléments traces (Ag, Cd, Cr, Cu, Mn, Ni, Pb et Se) dans les matrices biologiques solides. Water Research 1987; 21: 693–697
   Web of Science ®Google Scholar
• Banni M, Jebali J, Daubez M, Clerandeaux C, Guerbej H, Narbonne JF, Boussetta H. Monitoring pollution in Tunisian coasts: application of a classification scale based on biochemical markers. Biomarkers 2005; 10: 105–116
   PubMed Web of Science ®Google Scholar
• Banni M, Ben Dhiab R, El Abed A, Boussetta H. Genotoxicity, catalase and acetylcholinesterase in the assessment of the Tunisian coastal areas. Archive of Environmental Contamination and Toxicology 2003; 5: 157–165
   Google Scholar
• Barsyte D, White KN, Lovejoy DA. Cloning and characterization of metallothienein cDNAs in the mussel mytilus edulis L.digestive gland. Comparative Biochemistry and Physiology C 1999; 122: 287–296
   PubMed Web of Science ®Google Scholar
• Bebianno MJ, Company R, Serafima L, Camus L, Cosson RP, Fiala-Medoni A. Antioxidant systems and lipid peroxidation in Bathymodiolus azoricus from Mid-Atlantic Ridge hydrothermal vent fields. Aquatic Toxicology 2005; 75: 354–373
   PubMed Web of Science ®Google Scholar
• Bebianno MJ, Machado LJ. Concentrations of metals and metallothioneins in Mytilus galloprovincialis along the south coast of Portugal. Marine Pollution Bulletin 1997; 34: 666–671
   Web of Science ®Google Scholar
• Bradford MM. A rapid and sensitive method for the quanlication of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 1976; 72: 248–254
   PubMed Web of Science ®Google Scholar
• Bustin SA. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. Journal of Molecular Endocrinology 2002; 29: 23–39
   PubMed Web of Science ®Google Scholar
• Cavaletto M, Ghezzi A, Burlando B, Evangelisti V, Ceratto N, Viarengo A. Effect of hydrogen peroxide on antioxidant enzymes and metallothionein level in the digestive gland of Mytilus galloprovincialis. Comparative Biochemistry and Physiology Part C 2002; 131: 447–455
   PubMed Web of Science ®Google Scholar
• Dellali M, Gnassia Barelli M-B, Romeo M, Aissa P. The use of acetylcholinesterase activity in Ruditapes decussatus and Mytilus galloprovincialis in the biomonitoring of Bizerta lagoon. Comparative Biochemistry and Physiology Part C 2001; 130: 227–235
   PubMed Web of Science ®Google Scholar
• Dondero F, Dagnino A, Jonsson H, Capri F, Gastaldi L, Viarengo A. Assessing the occurrence of a stress syndrome in mussels (Mytilus edulis) using a combined biomarker/gene expression approach. Aquatic Toxicology 2006; 78S: S13–S24
   Google Scholar
• Dondero F, Piacentini L, Banni M, Rebelo M, Burlando B, Viarengo A. Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation. Gene 2005; 345: 259–270
   PubMed Web of Science ®Google Scholar
• Durnam DM, Palmiter R-D. Transcriptional regulation of the mouse metallothionein-I gene by heavy metals. Journal of Biological Chemistry 1981; 256: 5712–5716
   PubMed Web of Science ®Google Scholar
• Frazier JM. 1986. Cadmium–binding proteins in the mussel, mytilus edulis. Environmental Health Perspective 65:39–43.
   PubMed Web of Science ®Google Scholar
• Geret F, Serafim A, Bebianno MJ. Antioxidant enzyme activities, metallothioneins and lipid peroxidation as biomarkers in Ruditapes decussates. Ecotoxicology 2003; 12: 417–426
   PubMed Web of Science ®Google Scholar
• Halliwell B, Gutteridge J-M. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochemical Journal 1984; 219: 1–14
   PubMed Web of Science ®Google Scholar
• Hamza-Chaffai A, Cosson RP, Amiard Triquet C, El Abed A. Physicochemical forms of storage of metals (Cd, Cu and Zn) and metallothionein-like proteins in gills and liver of marine fish from Tunisian coast: ecotoxicological consequences. Comparative Biochemistry and Physiology Part C 1995; 111: 329–341
   PubMed Web of Science ®Google Scholar
• Hamza-Chaffai A, Pellerin J, Amiard JC. Health assessment of marine bivalve Ruditapes decussatus from the gulf of Gabès. Environment International 2003; 28: 609–617
   PubMed Web of Science ®Google Scholar
• Janero D. Malondialdehyde and thiobarbutiric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissues injury. Free Radical Biology and Medicine 1998; 9: 515–540
   Google Scholar
• Lemoine S, Bigot Y, Sellos D, Cosson R-P, Laulier M. Metallothionein isoforms in Mytilus edulis (Mollusca, Bivalvia): complementary DNA characterization and quantification of expression in different organs after exposure to cadmium, zinc, and copper. Marine Biotechnology 2000; 2: 195–203
   PubMed Web of Science ®Google Scholar
• Lemoine S, Laulier M. Potential use of the levels of the mRNA of a specific metallothionein isoform (MT-20) in mussel (Mytilus edulis) as a biomarker of cadmium contamination. Marine Pollution Bulletin 2003; 46: 1450–1455
   PubMed Web of Science ®Google Scholar
• Linde AR, Sanchez-Galan S, Vales-Mota P, Garcia Vazquez E. Metallothionein as bioindicator of fresh water metal pollution: European Eel and Brown trout. Ecotoxicology and Environmental Safety 1999; 40: 60–63
   Google Scholar
• Mackey EA, Overnell J, Dunbar B, Davidson I, Hunziker PE, Kagi JH, Fothergill JE. Complete amino acid sequences of five dimeric and four monomeric forms of metallotionein from the edible mussel mytilus edulis. European Journal of Biochemistry 1993; 218: 183–194
   PubMedGoogle Scholar
• Mazzucotelli A, Viarengo A, Canesi L, Ponzano E, Rivaro P. Determination of trace amounts of metalloprotein species in marine mussel samples by high-performance liquid chromatography with inductively coupled plasma atomic emission spectrometric detection. The Analyst 1991; 116: 605–608
   Web of Science ®Google Scholar
• Orlando C, Pinzani P, Pazzagli M. Developments in quantitative PCR. Clinical Chemistry and Laboratory Medicine 1998; 36: 255–269
   PubMed Web of Science ®Google Scholar
• Pfaffl M-W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research 2001; 29: e45
   PubMed Web of Science ®Google Scholar
• Pfaffl M-W, Horgan G-W, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research 2002; 30: e36
   PubMed Web of Science ®Google Scholar
• Rasmussen R. Quantification on the Light Cycler. Rapid Cycle Real-time PCR, Methods and Applications, S Meuer, C Wittwer, K Nakagawara. Springer-Verlag, New York 2001
   Google Scholar
• Rebelo MF, Pfeiffer W, Da Silva H, O Morales M. Cloning and detection of metallothionein mRNA by RT-PCR in mangrove oyster (Crassostrea rhizophorae). Aquatic Toxicology 2003; 64: 359–362
   PubMed Web of Science ®Google Scholar
• Roesijadi G, Bogumil R, Vasak M, Kagi JH. Modulation of DNA binding of a tramtrack zinc finger peptide by the metallothionein–thionein conjugate pair. Journal of Biological Chemistry 1998; 28: 17425–17432
   Google Scholar
• Roesijadi G, Unger ME. Immunochemical quantification of metallothioneins of a marine mollusc. Canadian Journal of Fish and Aquatic Science 1988; 45: 1257–1263
   Web of Science ®Google Scholar
• Romeo M, Hoarau P, Garello G, Gnassia-Barelli M, Girard JP. Mussel transplantation and biomarkers as useful tools for assessing water quality in the NW Mediterranean. Environmental Pollution 1998; 122: 369–378
   Google Scholar
• Sadhu C, Gedamu L. Regulation of human metallothionein (MT) genes. Differential expression of MTI-F, MTI-G, and MTII-A genes in the hepatoblastoma cell line (HepG2). Journal of Biological Chemistry 1988; 263: 2679–2684
   PubMed Web of Science ®Google Scholar
• Scheuhammer AM, Cherian GM. Quantification of metallothionein by a silver-saturation method. Toxicology and Applied Pharmacology 1985; 82: 417–425
   Web of Science ®Google Scholar
• Smaoui-Damak W, Hamza-Chaffai A, Berthet B, Amiard J-C. Preliminary study of the clam Ruditapes decussatus exposed in situ to metal contamination and originating from the Gulf of Gabés, Tunisia. Bulletin of Environmental Contamination and Toxicology 2003; 71: 961–970
   PubMed Web of Science ®Google Scholar
• Smaoui-Damak W, Hamza-Chaffaia A, Bebianno M-J, Amiard J-C. Variation of metallothioneins in gills of the clam Ruditapes decussates from the Gulf of Gabes (Tunisia). Comparative Biochemistry and Physiology Part C 2004; 139: 181–188
   PubMed Web of Science ®Google Scholar
• Sunderman FW, Marzoul A, Hopfer SM, Zaharia O, Reid MC. Increased lipid peroxidation in tissues of nickel chloride-treated rats. Annals of Clinical and Laboratory Science 1985; 15: 229–236
   PubMed Web of Science ®Google Scholar
• Thiele DJ. Metal regulated transcription in eukaryotes. Nucleic Acids Research 1992; 20: 1183–1191
   PubMed Web of Science ®Google Scholar
• Thompson JAJ, Cosson RP. An improve delectro-chemical method for the quantification of metallothionein in marine organisms. Marine Environmental Reaearch 1984; 11: 147–152
   Web of Science ®Google Scholar
• Tom M, Chen N, Segev M, Herut B, Rinkevich B. Quantifying fish metallothionein transcript by real time PCR for its utilization as an environmental biomarker. Marine Pollution Bulletin 2004; 48: 705–10
   PubMed Web of Science ®Google Scholar
• Viarengo A, Palermo S, Zanicchi G, Capelli R, Vaissiere R, Orunesu M. 1985a. Role of metallothioneins in Cu and Cd accumulation and elimination in the gill and digestive gland cells of Mytilus galloprovincialis Lam. Marine Environmental Research 23–36.
   Web of Science ®Google Scholar
• Viarengo A. Heavy metals in marine invertebrates, mechanisms of regulation and toxicity at cell level. CRC Critical Review in Aquatic Science 1989; 1: 295–317
   Google Scholar
• Viarengo A, Canesi L, Pertica M, Poli G, Moore M-N, Orunesu M. Heavy metal effect on lipid peroxidation in the tissues of Mytilus galloprovincialis L. Comparative Biochemistry and Physiology Part C 1990; 97: 37–42
   PubMed Web of Science ®Google Scholar
• Viarengo A, Nott JA. Mechanisms of heavy metals homeostasis in marine invertebrates. Comparative Biochemistry and Physiology Part C 1993; 104: 355–372
   Web of Science ®Google Scholar
• Viarengo A, Ponzano E, Dondero F, Fabbri R. A simple spectrophotometric method for metallothionein evaluation in marine organisms: an application to Mediterranean and Antarctic molluscs. Marine Environmental Research 1997; 44: 69–84
   Web of Science ®Google Scholar
• Viarengo A, Burlando B, Cavaletto M, Marchi B, Ponzano E, Blasco J. Role of metallothionein against oxidative stress in the mussel Mytilus galloprovincialis. American Journal of Physiology 1999a; 277: 1612–1619
   PubMed Web of Science ®Google Scholar
• Viarengo A, Burlando B, Dondero F, Marro A, Fabbri R. Metallothionein as a tool in biomonitoring programmes. Biomarkers 1999b; 4: 455–466
   PubMed Web of Science ®Google Scholar