The effects of anabolic-androgenic steroids on DNA damage in bodybuilders’ blood lymphocytes

References

• Abbasnezhad, A., et al., 2016. Nigella sativa improve redox homeostasis in heart and aorta of diabetic rat. Current nutrition & food science, 12 (1), 35–41.
   Web of Science ®Google Scholar
• Ahmed, M.A., 2015. Amelioration of nandrolone decanoate-induced testicular and sperm toxicity in rats by taurine: effects on steroidogenesis, redox and inflammatory cascades, and intrinsic apoptotic pathway. Toxicology and applied pharmacology, 282 (3), 285–296.
   PubMed Web of Science ®Google Scholar
• Aldomairy, A.F., 2013. Histological and ultrastructural study of the effect of nandrolone on the testis of adult male albino rat. Journal of American science, 35, 102–109.
   Google Scholar
• Angell, P.J., et al., 2012. Anabolic steroid use and longitudinal, radial, and circumferential cardiac motion. Medicine & science in sports & exercise, 44 (4), 583–590.
   PubMed Web of Science ®Google Scholar
• Arazi, H., Mohammadjafari, H., and Asadi, A., 2017. Use of anabolic androgenic steroids produces greater oxidative stress responses to resistance exercise in strength-trained men. Toxicology reports, 4, 282–286.
   PubMed Web of Science ®Google Scholar
• Aslanturk, Ö. S., 2018. In vitro cytotoxicity and cell viability assays: principles, advantages, and disadvantages. United Kingdom: InTech.
   Google Scholar
• Bahrke, M.S., and Yesalis, C.E., 2004. Abuse of anabolic androgenic steroids and related substances in sport and exercise. Current opinion in pharmacology, 4 (6), 614–620.
   PubMed Web of Science ®Google Scholar
• Bissegger, S., and Langlois, V.S., 2016. Androgens modulate gene expression and specific DNA methylation pattern of steroid 5α-reductases in the frog Silurana tropicalis. General and comparative endocrinology, 234, 123–132.
   PubMed Web of Science ®Google Scholar
• Bloomer, R.J., et al., 2005. Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. Journal of strength and conditioning research, 19 (2), 276–285.
   PubMed Web of Science ®Google Scholar
• Boada, L.D., et al., 1999. Evaluation of acute and chronic hepatotoxic effects exerted by anabolic-androgenic steroid stanozolol in adult male rats. Archives of toxicology, 73 (8-9), 465–472.
   PubMed Web of Science ®Google Scholar
• Cooper, C., et al., 2002. Exercise, free radicals and oxidative stress. Biochemical society transactions, 30 (2), 280–285.
   PubMed Web of Science ®Google Scholar
• Do Carmo, C.A., et al., 2012. Nandrolone androgenic hormone presents genotoxic effects in different cells of mice. Journal of applied toxicology : JAT, 32 (10), 810–814.
   PubMed Web of Science ®Google Scholar
• Finkel, T., and Holbrook, N.J., 2000. Oxidants, oxidative stress and the biology of ageing. Nature, 408 (6809), 239–247.
   PubMed Web of Science ®Google Scholar
• Foss, G.L., 1960. Some experiences with a new anabolic steroid (methandrostenolone). British medical journal, 1 (5182), 1300–1305.
   PubMedGoogle Scholar
• Harmer, P.A., 2010. Anabolic-androgenic steroid use among young male and female athletes: is the game to blame? British journal of sports medicine, 44 (1), 26–31.
   PubMed Web of Science ®Google Scholar
• Hildebrandt, T., Yehuda, R., and Alfano, L., 2011. What can allostasis tell us about anabolic-androgenic steroid addiction? Development and psychopathology, 23 (3), 907–919.
   PubMed Web of Science ®Google Scholar
• Kanayama, G., and Pope Jr, H.G., 2018. History and epidemiology of anabolic androgens in athletes and non-athletes. Molecular and cellular endocrinology, 464, 4–13.
   PubMed Web of Science ®Google Scholar
• Kara, M., et al., 2017. Determination of DNA damage and telomerase activity in stanozolol-treated rats. Experimental and therapeutic medicine, 13 (2), 614–618.
   PubMed Web of Science ®Google Scholar
• Karbalay‐Doust, S.N., et al., 2007. The reversibility of sperm quality after discontinuing nandrolone decanoate in adult male rats. Asian journal of andrology, 9 (2), 235–239.
   PubMed Web of Science ®Google Scholar
• Kianmehr, M., et al., 2016. DNA damage assessment in the lymphocytes of construction painters by comet assay. Toxicology and industrial health, 32 (11), 1902–1909.
   PubMed Web of Science ®Google Scholar
• Kianmehr, M., et al., 2019. Comparison of DNA damages in blood lymphocytes of indoor swimming pool lifeguards with non-lifeguards athletes. Mutation research. Genetic toxicology and environmental mutagenesis, 837, 29–33.
   PubMed Web of Science ®Google Scholar
• Kianmehr, M., Hajavi, J., and Gazeri, J., 2017. Assessment of DNA damage in blood lymphocytes of bakery workers by comet assay. Toxicology and industrial health, 33 (9), 726–735.
   PubMed Web of Science ®Google Scholar
• Lewis, M.I., et al., 2002. Role of IGF-I and IGF-binding proteins within diaphragm muscle in modulating the effects of nandrolone. American journal of physiology. Endocrinology and metabolism, 282 (2), E483–E490.
   PubMed Web of Science ®Google Scholar
• Maravelias, C., et al., 2005. Adverse effects of anabolic steroids in athletes. A constant threat. Toxicology letters, 158 (3), 167–175.
   PubMed Web of Science ®Google Scholar
• Martins, R.A., et al., 2010. Chromosome damage and cytotoxicity in oral mucosa cells after 2 months of exposure to anabolic steroids (decadurabolin and winstrol) in weight lifting. Steroids, 75 (12), 952–955.
   PubMed Web of Science ®Google Scholar
• Meireles, J.R.C., et al., 2013. Genotoxic and cytotoxic effects of testosterone cypionate (deposteron(®))®). Mutation research, 753 (2), 72–75.
   PubMed Web of Science ®Google Scholar
• Mohebbatia, R., et al., 2017. Hepatoprotective effects of combination hydroalcoholic extracts of Nigella Sativa and Curcuma Longa on adriamycin-induced oxidative stress in rat. Journal of reports in pharmaceutical sciences, 6, 93–102.
   Google Scholar
• Moller, P., 2006. Assessment of reference values for DNA damage detected by the comet assay in human blood cell DNA. Mutation research, 612 (2), 84–104.
   PubMed Web of Science ®Google Scholar
• Noorafshan, A., et al., 2005. High doses of nandrolone decanoate reduce volume of testis and length of seminiferous tubules in rats. APMIS, 113 (2), 122–125.
   PubMed Web of Science ®Google Scholar
• Parr, M.K., Flenker, U., and Schänzer, W., 2010. Sports-related issues and biochemistry of natural and synthetic anabolic substances. Endocrinology and metabolism clinics of North America, 39 (1), 45–57.
   PubMed Web of Science ®Google Scholar
• Parssinen, M., and Seppala, T., 2002. Steroid use and long-term health risks in former athletes. Sports medicine, 32, 83–94.
   PubMed Web of Science ®Google Scholar
• Pey, A., et al., 2003. Effects of prolonged stanozolol treatment on antioxidant enzyme activities, oxidative stress markers, and heat shock protein HSP72 levels in rat liver. The journal of steroid biochemistry and molecular biology, 87 (4-5), 269–277.
   PubMed Web of Science ®Google Scholar
• Pope, H.G., Jr., et al., 2014. Adverse health consequences of performance-enhancing drugs: an Endocrine Society scientific statement. Endocrine reviews, 35 (3), 341–375.
   PubMed Web of Science ®Google Scholar
• Riezzo, I., et al., 2011. Anabolic steroid- and exercise-induced cardio-depressant cytokines and myocardial β1 receptor expression in CD1 mice. Current pharmaceutical biotechnology, 12 (2), 275–284.
   PubMed Web of Science ®Google Scholar
• Salerno, M., et al., 2018. Anabolic androgenic steroids and carcinogenicity focusing on Leydig cell: a literature review. Oncotarget, 9 (27), 19415–19426.
   PubMedGoogle Scholar
• Shafi, F. a A., et al., 2020. Effect of exercise, synthetic anabolic steroids and protein intake on DNA damage in trained and untrained men. Meta gene, 24, 100685.
   Web of Science ®Google Scholar
• Shokri, S., et al., 2014. Combination of running exercise and high dose of anabolic androgenic steroid, nandrolone decanoate, increases protamine deficiency and DNA damage in rat spermatozoa. Andrologia, 46 (2), 184–190.
   PubMed Web of Science ®Google Scholar
• Singh, R.K., et al.,; Department of Biotechnology, IMS Engineering College, Ghaziabad, UP. 2010. Assessment of DNA damage by comet assay in lymphocytes of workers occupationally exposed to petroleum fumes. International journal of genetics, 2 (1), 18–22.
   Google Scholar
• Sinha-Hikim, I., et al., 2002. Testosterone-induced increase in muscle size in healthy young men is associated with muscle fiber hypertrophy. American journal of Physiology-Endocrinology and metabolism, 283 (1), E154–E164.
   PubMed Web of Science ®Google Scholar
• Souza, L.D.C.M., et al., 2017. Micronucleus as biomarkers of cancer risk in anabolic androgenic steroids users. Human & experimental toxicology, 36 (3), 302–310.
   PubMed Web of Science ®Google Scholar
• Straif, K., et al., 2009. A review of human carcinogens–part C: metals, arsenic, dusts, and fibres. The lancet oncology, 10 (5), 453–454.
   PubMed Web of Science ®Google Scholar
• Sumida, S., et al., 1997. Effect of a single bout of exercise and beta-carotene supplementation on the urinary excretion of 8-hydroxy-deoxyguanosine in humans. Free radical research, 27 (6), 607–618.
   PubMed Web of Science ®Google Scholar
• Torres-Bugarin, O., et al., 2007. Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders. British journal of sports medicine, 41 (9), 592–596.
   PubMed Web of Science ®Google Scholar
• Tricker, R., O'neill, M.R., and Cook, D., 1989. The incidence of anabolic steroid use among competitive bodybuilders. Journal of drug education, 19 (4), 313–325.
   PubMed Web of Science ®Google Scholar