Imaging living cells of Aspergillus in vitro

References

• Murphy DB. Fundamentals of Light Microscopy and Electronic Imaging. Wiley-Liss, New York 2001
   Google Scholar
• Freitag M, Hickey PC, Raju NB, Selker EU, Read ND. GFP as a tool to analyze the organization, dynamics and function of nuclei and microtubules in Neurospora crassa. Fungal Genet Biol 2004; 41: 897–910
   PubMed Web of Science ®Google Scholar
• Hickey PC, Swift SR, Roca MG, Read ND. Live-cell imaging of filamentous fungi using vital fluorescent dyes and confocal microscopy. Methods in Microbiology, Vol 35, Microbial Imaging, T Savidge, C Pothoulakis. Elsevier, London 2005; 63–87
   Google Scholar
• Mouriño-Pérez RR, Roberson RW, Bartnicki-Garcia S. Microtubule dynamics and organization during hyphal growth and branching in Neurospora crassa. Fungal Genet Biol 2006; 43: 389–400
   PubMed Web of Science ®Google Scholar
• Uchida, M, Mouriño-Pérez, RR, Freitag, M, Bartnicki-Garcia, S. Microtubule dynamics and the role of molecular motors in Neurospora crassa. Fungal Genet Biol 2007, Oct 26; 45: 683–692.
   PubMed Web of Science ®Google Scholar
• Horio T, Oakley BR. The role of microtubules in rapid hyphal tip growth of Aspergillus nidulans. Mol Biol Cell 2005; 16: 918–926
   PubMed Web of Science ®Google Scholar
• Osmani Ah, Davies J, Liu H-L, Nile A, Osmani SA. Systematic deletion and mitotic localization of the nuclear pore complex proteins of Aspergillus nidulans. Mol Biol Cell 2006; 17: 4946–4961
   PubMed Web of Science ®Google Scholar
• Taheri-Talesh N, Horio T, Araujo-Bazán L, et al. The tip growth apparatus of Aspergillus nidulans. Mol Biol Cell 2008; 19: 1439–1449
   PubMed Web of Science ®Google Scholar
• Swedlow JR, Platani M. Live cell imaging using wide-field microscopy and deconvolution. Cell Struct Function 2002; 27: 335–341
   PubMed Web of Science ®Google Scholar
• Valkonen M, Kalkman ER, Saloheimo M, et al. Spatially segregated SNARE protein interactions in living fungal cells. J Biol Chem 2007; 282: 22775–22785
   PubMed Web of Science ®Google Scholar
• Riquelme M, Bartnicki-Garcia S, Gonzalez-Prieto JM, et al. Spitzenkörper localization and intracellular traffic of green fluorescent protein-labeled CHS-3 and CHS-6 chitin synthases in living hyphae of Neurospora crassa. Eukaryot Cell 2007; 6: 1853–1864
   PubMedGoogle Scholar
• Czymmek KH, Bourett TM, Howard RJ. Fluorescent protein probes in fungi. Methods in Microbiology, Vol. 35, Microbial Imaging, T Savidge, C Pothoulakis. Elsevier, London 2005; 27–62
   Google Scholar
• Vinck A, Terlou M, Pestman WR, et al. Hyphal differentiation in the exploring mycelium of Aspergillus niger. Mol Microbiol 2005; 58: 693–699
   PubMed Web of Science ®Google Scholar
• Purschwitz J, Müller S, Kastener C, et al. Functional and physical interaction of blue- and red-light sensors in Aspergillus nidulans. Curr Biol 2008; 26: 255–259
   Google Scholar
• Efimov VP, Zhang J, Xiang X. CLIP-170 homologue and NUDE plays overlapping roles in NUDF localization in Aspergillus nidulans. Curr Biol 2006; 17: 2021–2034
   Google Scholar
• Han G, Liu B, Zhang W, Morris NR, Xiang X. The Aspergillus cytoplasmic dynein heavy chain and NUDF localize to microtubule ends and affect microtubule dynamics. Curr Biol 2001; 11: 719–724
   PubMed Web of Science ®Google Scholar
• Horio T, Oakley BR. The role of microtubules in rapid hyphal tip growth of Aspergillus nidulans. Mol Biol Cell 2005; 16: 918–926
   PubMed Web of Science ®Google Scholar
• Konzack S, Rischitor PE, Enke C, Fischer R. The role of the kinesin motor KipA in microtubule organization and polarized growth of Aspergillus nidulans. Mol Biol Cell 2005; 16: 497–506
   PubMed Web of Science ®Google Scholar
• Li S, Oakley CE, Chen G, et al. Cytoplasmic dynein's mitotic spindle pole localization requires a functional anaphase-promoting complex, gamma-tubulin, and NUDF/LIS1 in Aspergillus nidulans. Mol Biol Cell 2005; 16: 3591–3605
   PubMed Web of Science ®Google Scholar
• Liu B, Xiang X, Lee YR. The requirement of the LC8 dynein light chain for nuclear migration and septum positioning is termperature dependent in Aspergillus nidulans. Mol Microbiol 2003; 47: 291–301
   PubMed Web of Science ®Google Scholar
• Osmani AH, Davies J, Oakley CE, Oakley BR, Osmani SA. TINA interacts with the NIMA kinase in Aspergillus nidulans and negatively regulates astral microtubules during metaphase arrest. Mol Biol Cell 2003; 14: 3169–3179
   PubMed Web of Science ®Google Scholar
• Ovechkina Y, Maddox P, Oakley CE, et al. Spindle formation in Aspergillus is coupled to tubulin movement into the nucleus. Mol Biol Cell 2003; 14: 2192–2200
   PubMed Web of Science ®Google Scholar
• Prigozhina NL, Oakley CE, Lewis AM, et al. Gamma-tubulin plays an essential role in the coordination of mitotic events. Mol Biol Cell 2004; 15: 1374–1386
   PubMed Web of Science ®Google Scholar
• Su W, Li S, Oakley BR, Xiang X. Dual-Color imaging of nuclear division and mitotic spindle elongation in live cells of Aspergillus nidulans. Eukaryot Cell 2004; 3: 553–556
   PubMedGoogle Scholar
• Veith D, Scherr N, Efimov VP, Fischer R. Role of the spindle-pole-body protein ApsB and the cortex protein ApsA in microtubule organization and nuclear migration in Aspergillus nidulans. J Cell Sci 2005; 118: 3705–3716
   PubMed Web of Science ®Google Scholar
• Virag A, Lee MP, Si H, Harris SD. Regulation of hyphal morphogenesis by cdc42 and rac1 homologues in Aspergillus nidulans. Mol Microbiol 2007; 66: 1579–1596
   PubMed Web of Science ®Google Scholar
• Xiang X, Han G, Winkelmann DA, Zuo W, Morris NR. Dynamics of cytoplasmic dynein in living cells and the effect of a mutation in the dynactin complex actin-related protein Arp1. Curr Biol 2000; 10: 603–606
   PubMedGoogle Scholar
• Xiang X, Fischer R. Nuclear migration and positioning in filamentous fungi. Fungal Genet Biol 2004; 41: 411–419
   PubMed Web of Science ®Google Scholar
• Zhang J, Han G, Xiang X. Cytoplasmic dynein intermediate and heavy chain are dependent upon each other for microtubule end localization in Aspergillus nidulans. Mol Microbiol 2002; 44: 381–392
   PubMed Web of Science ®Google Scholar
• Zhang J, Li S, Fischer R, Xiang X. Accumulation of cytoplasmic dynein and dynactin at microtubule plus ends in Aspergillus nidulans is kinesin dependent. Mol Biol Cell 2003; 14: 1479–1488
   PubMed Web of Science ®Google Scholar
• Fernández-Abalos JM, Fox H, Pitt C, Wells B, Doonan JH. Plant-adapted green fluorescent protein is a versatile vital reporter for gene expression, protein localization and mitosis in the filamentous fungus, Aspergillus nidulans. Mol Microbiol 1998; 27: 121–130
   PubMed Web of Science ®Google Scholar
• Maruyama J-I, Kikuchi S, Kitamoto K. Differential distribution of the endoplasmic reticulum network as visualized by the BipA-EGFP fusion protein in hyphal compartments across the septum of the filamentous fungus, Aspergillus oryzae. Fungal Genet Biol 2006; 43: 642–654
   PubMed Web of Science ®Google Scholar
• Kuratsu M, Taura A, Shoji J-Y, et al. Systematic analysis of SNARE localization in the filamentous fungus Aspergillus oryzae. Fungal Genet Biol 2007; 44: 1310–1323
   PubMed Web of Science ®Google Scholar
• Breakspear A, Langford KJ, Momany M, Assinder SJ. CopA:GFP localizes to putative Golgi equivalents in Aspergillus nidulans. FEMS Microbiol Lett 2007; 277: 90–97
   Google Scholar
• Toews MW, Warmbold J, Konzack S, et al. Establishment of mRFP1 as a fluorescent marker in Aspergillus nidulans and construction of expression vectors for high-throughput protein tagging using recombination in vitro (GATEWAY). Curr Genet 2004; 45: 383–389
   PubMed Web of Science ®Google Scholar
• Bhabhra R, Zhao W, Rhodes JC, Askew DS. Nucleolar localization of Aspergillus fumigatus CGrA is temperature-dependent. Fungal Genet Biol 2006; 43: 1–7
   PubMed Web of Science ®Google Scholar
• De Souza CPC, Osmani AH, Hashmi SB, Osmani SA. Partial nuclear pore complex disassembly during closed mitosis in Aspergillus nidulans. Curr Biol 2004; 14: 1973–1984
   PubMed Web of Science ®Google Scholar
• Fox H, Hickey PC, Fernández-Ábalos JM, Lunness P, Read ND, Doonan JH. Dynamic distribution of BIMG PP1 in living hyphae of Aspergillus indicates a novel role in septum formation. Mol Microbiol 2002; 45: 1219–1230
   PubMed Web of Science ®Google Scholar
• Shaw BD, Upadhyay S. Aspergillus nidulans swoK encodes an RNA binding protein that is important for cell polarity. Fungal Genet Biol 2005; 42: 862–872
   PubMed Web of Science ®Google Scholar
• Takeshita N, Higashitsuji Y, Konzack S, Fischer R. Apical sterol-rich membranes are essential for localizing cell end markers that determine growth directionality in the filamentous fungus Aspergillus nidulans. Mol Biol Cell 2008; 19: 339–351
   PubMed Web of Science ®Google Scholar
• Blumenstein A, Vienken K, Tasler R, et al. The Aspergillus nidulans phytochrome FphA represses sexual development in red light. Curr Biol 2005; 15: 1833–1888
   PubMed Web of Science ®Google Scholar
• Gordon CL, Archer DB, Geenes DJ, et al. A glucoamylase:GFP gene fusion to study protein secretion by individual hyphae of Aspergillus niger. J Microbiol Meth 2000; 42: 39–48
   PubMed Web of Science ®Google Scholar
• Sharpless KE, Harris SD. Functional characterization and localization of the Aspergillus nidulans forming SEPA. Mol Biol Cell Feb 2002; 13: 469–479
   PubMed Web of Science ®Google Scholar
• Maruyama J, Juvvadi PR, Ishi K, Kitamoto K. Three-dimensional image analysis of plugging at the septal pore by Woronin body during hypotonic shock inducing hyphal tip bursting in the filamentous fungus Aspergillus oryzae. Biochem Biophys Res Commun 2005; 17: 1081–1088
   Google Scholar
• Harris SD, Read ND, Roberson RW, et al. Polarisome meets Spitzenkörper: microscopy, genetics, and genomics converge. Eukaryot Cell 2005; 4: 225–229
   PubMedGoogle Scholar
• Fischer-Parton S, Parton RM, Hickey PC, et al. Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae. J Microsc 2000; 198: 246–259
   PubMed Web of Science ®Google Scholar
• Meyer V, Arentshorst M, van den Hondel CAMJJ, Ram AFJ. The polarisome component Spa localises to hyphal tips of Aspergillus niger and is important for polar growth. Fungal Genet Biol 2008; 45: 152–164
   PubMed Web of Science ®Google Scholar
• Ohneda M, Arioka M, Nakajima H, Kitamoto K. Visualization of vacuoles in Aspergillus oryzae by expression of CPY-EGFP. Fungal Genet Biol 2002; 37: 29–38
   PubMed Web of Science ®Google Scholar
• Shoji J-Y, Arioka M, Kitamoto K. Vacuolar membrane dynamics in the filamentous fungus Aspergillus oryzae. Eukaryot Cell 2006; 5: 411–421
   PubMed Web of Science ®Google Scholar
• Juvvadi PR, Maruyama J, Kitamoto K. Phosphorylation of the Aspergillus oryzae Woronin body protein, AoHex1, by protein kinase C: evidence for its role in the multimerization and proper localization of the Woronin body protein. Biochem J 2007; 405: 533–540
   PubMed Web of Science ®Google Scholar
• Czymmek KJ, Whallon JH, Klomparens A. Confocal microscopy in mycological research. Exp Mycol 1994; 18: 275–293
   Google Scholar
• Jaffe LE. Calcium waves. Philos Trans R Soc Lond B Biol Sci 2008; 363: 1311–1316
   PubMed Web of Science ®Google Scholar
• Parry H, McDougall A, Whitaker M. Microdomains bounded by endoplasmic reticulum segregate cell cycle calcium transients in syncytial Drosophila embryos. J Cell Biol 2005; 171: 47–59
   PubMed Web of Science ®Google Scholar
• Markham P. Organelles of filamentous fungi. The Growing Fungus, NAR Gow, GM Gadd. Chapman & Hall, London 1995; 75–98
   Google Scholar
• Swift SR, Hart CA, Bartlett DW, Read ND. Interactions between azoxystrobin and Puccinia recondita, Erysiphe graminis, and Botrytis cinerea on the microscale. Scanning 2001; 23: 153–154
   Google Scholar
• Cole L, Orlovich DA, Ashford AE. Structure, function, and motility of vacuoles in filamentous fungi. Fungal Genet Biol 1998; 24: 86–100
   PubMed Web of Science ®Google Scholar
• Darrah PR, Tlalka M, Ashford A, Watkinson SC, Fricker MD. The vacuole system is a significant intracellular pathway for longitudinal solute transport in basidiomycete fungi. Eukaryot Cell 2006; 5: 1111–1125
   PubMedGoogle Scholar
• Howard RJ. Ultrastructure analysis of hyphal tip cell growth in fungi: Spitzenkörper, cytoskeleton and endomembranes after freeze-substitution. J Cell Sci 1981; 48: 89–103
   PubMed Web of Science ®Google Scholar