Aspergillus PCR formidable challenges and progress

References

• Chanock SJ, Pizzo PA. Infectious complications of patients undergoing therapy for acute leukemia: current status and future prospects. Semin Oncol 1997; 2: 132–140
   Google Scholar
• Wald A, Leisenring W, van Burik JA, et al. Epidemiology of Aspergillus infections in a large cohort of patients undergoing bone marrow transplantation. J Infect Dis 1997; 175: 1459–1466
   PubMed Web of Science ®Google Scholar
• Denning DW, Marinus A, Cohen J, et al. An EORTC multicentre prospective survey of invasive aspergillosis in haematological patients: diagnosis and therapeutic outcome. J Infect 1998; 37: 173–180
   PubMed Web of Science ®Google Scholar
• Morgan J, Wannemuehler KA, Marr KA, et al. Incidence of invasive aspergillosis following hematopoietic stem cell and solid organ transplantation: interim results of a prospective multicenter surveillance program. Med Mycol 2005; 43: S49–S58
   PubMed Web of Science ®Google Scholar
• Meyer MH, Letscher-Bru V, Jaulhac B, et al. Comparison of mycosis IC / F and plus aerobic / F media for diagnosis of fungemia by the Bactec 9240 system. J Clin Microbiol 2004; 42: 773–777
   PubMed Web of Science ®Google Scholar
• McDonald LC, Weinstein MP, Fune J, et al. Controlled comparison of BacT / ALERT FANaerobic medium and BACTEC fungal blood culture medium for detection of fungemia. J Clin Microbiol 2001; 39: 622–624
   PubMed Web of Science ®Google Scholar
• Verweij PE, Meis JF. Microbiological diagnosis of invasive fungal infections in transplant recipients. Transpl Infect Dis 2000; 2: 80–87
   PubMedGoogle Scholar
• Kim MJ, Lee KS, Kim J, et al. Crescent sign in invasive pulmonary aspergillosis: frequency and related CT and clinical factors. J Comput Assist Tomogr 2001; 25: 305–310
   PubMed Web of Science ®Google Scholar
• Caillot D, Couaillier JF, Bernard A, et al. Increasing volume and changing characteristics of invasive pulmonary aspergillosis on sequential thoracic computed tomography scans in patients with neutropenia. J Clin Oncol 2001; 19: 253–259
   PubMed Web of Science ®Google Scholar
• Horger M, Einsele H, Schumacher U, et al. Invasive pulmonary aspergillosis: frequency and meaning of the ‘hypodense sign’ on unenhanced CT. Br J Radiol 2005; 55: 437–444
   Google Scholar
• Ascioglu S, Rex JH, de Pauw B, et al. Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: an international consensus. Clin Infect Dis 2002; 34: 7–14
   PubMed Web of Science ®Google Scholar
• Miyazaki T, Kohno S, Mitsutake K, et al. Plasma (1– > 3)-beta-D-glucan and fungal antigenemia in patients with candidemia, aspergillosis, and cryptococcosis. J Clin Microbiol 1995; 33: 3115–3118
   PubMed Web of Science ®Google Scholar
• Odabasi Z, Mattiuzzi G, Estey E, et al. Beta-D-glucan as a diagnostic adjunct for invasive fungal infections: validation, cutoff development, and performance in patients with acute myelogenous leukemia and myelodysplastic syndrome. Clin Infect Dis 2004; 39: 199–205
   PubMed Web of Science ®Google Scholar
• Einsele H, Hebert H, Roller G, et al. Detection and identification of fungal pathogens in blood by using molecular probes. J Clin Microbiol 1997; 35: 1353–1360
   PubMed Web of Science ®Google Scholar
• Loeffler J, Hebart H, Sepe S, et al. Detection of PCR-amplified fungal DNA by using a PCR-ELISA system. Med Mycol 1998; 36: 275–279
   PubMed Web of Science ®Google Scholar
• Kami M., Fukui T., Ogawa S., et al. Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis. Clin Infect Dis 2001; 33: 1504–1512
   PubMed Web of Science ®Google Scholar
• Lass-Florl C, Gunsilius E, Gastl G, et al. Clinical evaluation of Aspergillus-PCR for detection of invasive aspergillosis in immunosuppressed patients. Mycoses 2005; 48: S12–S7
   PubMed Web of Science ®Google Scholar
• Klingspor L, Jalal S. Molecular detection and identification of Candida and Aspergillus spp. from clinical samples using real-time PCR. Clin Microbiol Infect 2006; 12: 745–753
   PubMed Web of Science ®Google Scholar
• Lewis White P, Barnes RA. Aspergillus PCR – Platforms, strengths and weaknesses. Med Mycol 2006; 44: S191–S198
   Web of Science ®Google Scholar
• Schabereiter-Gurtner C, Selitsch B, Rotter ML, et al. Development of novel real-time PCR assays for detection and differentiation of eleven medically important Aspergillus and Candida species in clinical specimens. J Clin Microbiol 2007; 45: 906–914
   PubMed Web of Science ®Google Scholar
• Simoneau E, Kelly M, Labbe AC, et al. What is the clinical significance of positive blood cultures with Aspergillus sp in hematopoietic stem cell transplant recipients? A 23 year experience. Bone Marrow Transplant 2005; 35: 303–306
   PubMed Web of Science ®Google Scholar
• Loeffler J, Hebart H, Brauchle U, et al. Comparison between plasma and whole blood specimens for detection of Aspergillus DNA by PCR. J Clin Microbiol 2000; 38: 3830–3833
   PubMed Web of Science ®Google Scholar
• White PL, Barton R, Guiver M, et al. A consensus on fungal polymerase chain reaction diagnosis?: a United Kingdom-Ireland evaluation of polymerase chain reaction methods for detection of systemic fungal infections. J Mol Diagn 2006; 8: 376–384
   PubMed Web of Science ®Google Scholar
• Garcia ME, Blanco JL, Caballero J, Gargallo-Viola D. Anticoagulants interfere with PCR used to diagnose invasive aspergillosis. J Clin Microbiol 2002; 40: 1567–1568
   PubMed Web of Science ®Google Scholar
• Paterson PJ, Seaton S, McLaughlin J, Kibbler CC. Development of molecular methods for the identification of Aspergillus and emerging moulds in paraffin wax embedded tissue sections. Mol Pathol 2003; 56: 368–370
   PubMedGoogle Scholar
• Griffiths LJ, Anyim M, Doffman SR, et al. Comparison of DNA extraction methods for Aspergillus, fumigatus using real-time PCR. J Med Microbiol 2006; 55: 1187–1191
   PubMed Web of Science ®Google Scholar
• Rimek D, Garg AP, Haas WH, Kappe R. Identification of contaminating fungal DNA sequences in Zymolyase. J Clin Microbiol 1999; 37: 830–831
   PubMed Web of Science ®Google Scholar
• Loeffler J, Herbart H, Bialek R, et al. Contaminations occurring in fungal PCR assays. J Clin Microbiol 2000; 38: 3830–3833
   PubMed Web of Science ®Google Scholar
• Rickerts V, Just-Nübling G, Konrad F, et al. Diagnosis of invasive aspergillosis and mucormucosis in immunocompromised patients by seminested PCR assay of tissue samples. Eur J Clin Microbiol Infect Dis 2006; 25: 8–13
   PubMed Web of Science ®Google Scholar
• Williamson EC, Leeming JP, Palmer HM, et al. Diagnosis of invasive aspergillosis in bone marrow transplant recipients by polymerase chain reaction. Br J Haematol 2000; 108: 132–139
   PubMed Web of Science ®Google Scholar
• White PL, Linton CJ, Perry MD, Johnson EM, Barnes RA. The evolution and evaluation of a whole blood polymerase chain reaction assay for the detection of invasive aspergillosis in hematology patients in a routine clinical setting. Clin Infect Dis 2006; 42: 479–486
   PubMed Web of Science ®Google Scholar
• Lau A, Chen S, Sorrell T, et al. Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens. J Clin Microbiol 2007; 45: 380–385
   PubMed Web of Science ®Google Scholar
• Florent M, Katsahian S, Vekhoff A, et al. Prospective evaluation of a polymerase chain reaction-ELISA targeted to Aspergillus, fumigatus and Aspergillus, flavus for the early diagnosis of invasive aspergillosis in patients with hematological malignancies. J Infect Dis 2006; 193: 741–747
   PubMed Web of Science ®Google Scholar
• Buchheidt D, Hummel M, Schleiermacher D, et al. Prospective clinical evaluation of a LightCycler-mediated polymerase chain reaction assay, a nested-PCR assay and a galactomannan enzyme-linked immunosorbent assay for detection of invasive aspergillosis in neutropenic cancer patients and haematological stem cell transplant recipients. Br J Haematol 2004; 125: 196–202
   PubMed Web of Science ®Google Scholar
• Kawazu M, Kanda Y, Nannya Y, et al. Prospective comparison of the diagnostic potential of real-time PCR, double-sandwich enzyme-linked immunosorbent assay for galactomannan, and a (1– > 3)-beta-D-glucan test in weekly screening for invasive aspergillosis in patients with hematological disorders. J Clin Microbiol 2004; 42: 2733–2741
   PubMed Web of Science ®Google Scholar
• Hebart H, Löffler J, Meisner C, et al. Early detection of Aspergillus infection after allogeneic stem cell transplantation by polymerase chain reaction screening. J Infect Dis 2000; 181: 1713–1719
   PubMed Web of Science ®Google Scholar
• Jensen HE, Salonen J, Ekfors TO. The use of immunohistochemistry to improve sensitivity and specificity in the diagnosis of systemic mycoses in patients with haematological malignancies. J Pathol 1997; 181: 100–105
   PubMed Web of Science ®Google Scholar
• Walsh TJ, Finberg RW, Arndt C, et al. Liposomal amphotericin B for empirical therapy in patients with persistent fever and neutropenia. N Eng J Med 1999; 340: 764–771
   PubMed Web of Science ®Google Scholar
• Buchheidt D, Baust C, Skladny H, et al. Clinical evaluation of a polymerase chain reaction assay to detect Aspergillus species in bronchoalveolar lavage samples of neutropenic patients. Br J Haematol 2002; 116: 803–811
   PubMed Web of Science ®Google Scholar
• Francesconi A, Kasai M, Petraitiene R, et al. Characterization and comparison of galactomannan enzyme immunoassay and quantitative real-time PCR assay for detection of Aspergillus, fumigatus in bronchoalveolar lavage fluid from experimental invasive pulmonary aspergillosis. J Clin Microbiol 2006; 44: 2475–2480
   PubMed Web of Science ®Google Scholar
• Raad I, Hanna H, Huaringa A, et al. Diagnosis of invasive pulmonary aspergillosis using polymerase chain reaction-based detection of Aspergillus in BAL. Chest 2002; 121: 1171–1176
   PubMed Web of Science ®Google Scholar
• Hummel M, Spiess B, Kentouche K, et al. Detection of Aspergillus DNA in cerebrospinal fluid from patients with cerebral aspergillosis by a nested PCR assay. J Clin Microbiol 2006; 44: 3989–3993
   PubMed Web of Science ®Google Scholar
• Khan ZU, Ahmad S, Mokaddas E, et al. Cerebral aspergillosis diagnosed by detection of Aspergillus, flavus-specific DNA, galactomannan and (1– > 3)-beta-D-glucan in clinical specimens. J Med Microbiol 2007; 56: 129–132
   PubMed Web of Science ®Google Scholar