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Review Article

A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications

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Pages 620-635 | Received 13 Jun 2016, Accepted 15 Nov 2016, Published online: 12 Dec 2016

References

  • Ashour ML, El-Readi M, Youns M, Mulyaningsih S, Sporer F, Efferth T, Wink M. 2009. Chemical composition and biological activity of the essential oil obtained from Bupleurum marginatum (Apiaceae). J Pharm Pharmacol. 61:1079–1087.
  • Ashour ML, El-Readi MZ, Hamoud R, Eid SY, El AS, Nibret E, Herrmann F, Youns M, Tahrani A, Kaufmann D, et al. 2014. Anti-infective and cytotoxic properties of Bupleurum marginatum. Chin Med. 9:4
  • Ashour ML, Wink M. 2011. Genus Bupleurum: a review of its phytochemistry, pharmacology and modes of action. J Pharm Pharmacol. 63:305–321.
  • Bannon A, Zhang SD, Schock BC, Ennis M. 2015. Cystic fibrosis from laboratory to bedside: the role of A20 in NF-κB-mediated inflammation. Med Princ Pract. 24:301–310.
  • Cao SL, Chen E, Zhang QZ, Jiang XG. 2007. A novel nasal delivery system of a Chinese traditional medicine, Radix Bupleuri, based on the concept of ion-activated in situ gel. Arch Pharm Res. 30:1014–1019.
  • Chen E, Chen J, Cao SL, Zhang QZ, Jiang XG. 2010. Preparation of nasal temperature-sensitive in situ gel of Radix Bupleuri and evaluation of the febrile response mechanism. Drug Dev Ind Pharm. 36:490–496.
  • Chen J, Duan M, Zhao Y, Ling F, Xiao K, Li Q, Li B, Lu C, Qi W, Zeng Z, et al. 2015. Saikosaponin A inhibits influenza A virus replication and lung immunopathology. Oncotarget. 6:42541–42556.
  • Chen L, Zhang F, Kong D, Zhu X, Chen W, Wang A, Zheng S. 2013a. Saikosaponin D disrupts platelet-derived growth factor-β receptor/p38 pathway leading to mitochondrial apoptosis in human LO2 hepatocyte cells: a potential mechanism of hepatotoxicity. Chem Biol Interact. 206:76–82.
  • Chen MF, Huang CC, Liu PS, Chen CH, Shiu LY. 2013b. Saikosaponin A and saikosaponin D inhibit proliferation and migratory activity of rat HSC-T6 cells. J Med Food. 16:793–800.
  • Chen SM, Sato N, Yoshida M, Satoh N, Ueda S. 2008. Effects of Bupleurum scorzoneraefolium, Bupleurum falcatum, and saponins on nephrotoxic serum nephritis in mice. J Ethnopharmacol. 116:397–402.
  • Chen X, Liu X, Li Z, Sun M, Chu D, Sun Y. 1981. The preliminary research on the toxicity of Bupleurum longiradiatum. Chin Pharm J. 1:62.
  • Chen X, Yu T, Chen Z, Zhao R, Mao S. 2014. Effect of saikosaponins and extracts of vinegar-baked Bupleuri Radix on the activity of β-glucuronidase. Xenobiotica. 44:785–791.
  • Chen Y, Wang J, Yuan L, Zhou L, Jia X, Tan X. 2011. Interaction of the main components from the traditional Chinese drug pair Chaihu-Shaoyao based on rat intestinal absorption. Molecules. 16:9600–9610.
  • Chen YL, Lin SZ, Chang WL, Cheng YL, Harn HJ. 2005. Requirement for ERK activation in acetone extract identified from Bupleurum scorzonerifolium induced A549 tumor cell apoptosis and keratin 8 phosphorylation. Life Sci. 76:2409–2420.
  • Cheng PW, Ng LT, Chiang LC, Lin CC. 2006. Antiviral effects of saikosaponins on human coronavirus 229E in vitro. Clin Exp Pharmacol Physiol. 33:612–616.
  • Cheng YL, Chang WL, Lee SC, Liu YG, Lin HC, Chen CJ, Yen CY, Yu DS, Lin SZ, Harn HJ. 2003. Acetone extract of Bupleurum scorzonerifolium inhibits proliferation of A549 human lung cancer cells via inducing apoptosis and suppressing telomerase activity. Life Sci. 73:2383–2394.
  • Cheng YL, Lee SC, Lin SZ, Chang WL, Chen YL, Tsai NM, Liu YC, Tzao C, Yu DS, Harn HJ. 2005. Anti-proliferative activity of Bupleurum scrozonerifolium in A549 human lung cancer cells in vitro and in vivo. Cancer Lett. 222:183–193.
  • Chiang LC, Ng LT, Liu LT, Shieh DE, Lin CC. 2003. Cytotoxicity and anti-hepatitis B virus activities of saikosaponins from Bupleurum species. Planta Med. 69:705–709.
  • Chou CC, Pan SL, Teng CM, Guh JH. 2003. Pharmacological evaluation of several major ingredients of Chinese herbal medicines in human hepatoma Hep3B cells. Eur J Pharm Sci. 19:403–412.
  • Dang SS, Wang BF, Cheng YA, Song P, Liu ZG, Li ZF. 2007. Inhibitory effects of saikosaponin-D on CCl4-induced hepatic fibrogenesis in rats. World J Gastroenterol. 13:557–563.
  • DI HY, Zhang YY, Chen DF. 2013. Isolation of an anti-complementary polysaccharide from the root of Bupleurum chinense and identification of its targets in complement activation cascade. Chin J Nat Med. 11:177–184.
  • Ebata N, Nakajima K, Hayashi K, Okada M, Maruno M. 1996. Saponins from the root of Bupleurum falcatum. Phytochemistry. 41:895–901.
  • Food and Drug Administration of Gansu Province. 2008. Chinese medicinal materials standard of Gansu province. Lanzhou, China: Gansu Culture Press.
  • Fu Y, Hu X, Cao Y, Zhang Z, Zhang N. 2015. Saikosaponin A inhibits lipopolysaccharide-oxidative stress and inflammation in Human umbilical vein endothelial cells via preventing TLR4 translocation into lipid rafts. Free Radic Biol Med. 89:777–785.
  • Han NR, Kim HM, Jeong HJ. 2011. Inactivation of cystein-aspartic acid protease (caspase)-1 by saikosaponin A. Biol Pharm Bull. 34:817–823.
  • Hao Y, Piao X, Piao X. 2012. Saikosaponin-d inhibits β-conglycinin induced activation of rat basophilic leukemia-2H3 cells. Int Immunopharmacol. 13:257–263.
  • He D, Wang H, Xu L, Wang X, Peng K, Wang L, Liu P, Qu P. 2016. Saikosaponin A attenuates oxidized LDL uptake and prompts cholesterol efflux in THP-1 cells. J Cardiovasc Pharmacol. 67:510–518.
  • He S, Lu G, Hou H, Zhao Z, Zhu Z, Lu X, Chen J, Wang Z. 2014. Saikosaponin-d suppresses the expression of cyclooxygenase-2 through the phospho-signal transducer and activator of transcription 3/hypoxia-inducible factor-1α pathway in hepatocellular carcinoma cells. Mol Med Rep. 10:2556–2562.
  • Hsu YL, Kuo PL, Lin CC. 2004a. The proliferative inhibition and apoptotic mechanism of saikosaponin D in human non-small cell lung cancer A549 cells. Life Sci. 75:1231–1242.
  • Hsu YL, Kuo PL, Weng TC, Yen MH, Chiang LC, Lin CC. 2004b. The antiproliferative activity of saponin-enriched fraction from Bupleurum kaoi is through Fas-dependent apoptotic pathway in human non-small cell lung cancer A549 cells. Biol Pharm Bull. 27:1112–1115.
  • Hu SC, Lee IT, Yen MH, Lin CC, Lee CW, Yen FL. 2016. Anti-melanoma activity of Bupleurum chinense, Bupleurum kaoi and nanoparticle formulation of their major bioactive compound saikosaponin-d. J Ethnopharmacol. 179:432–442.
  • Hu Y, Pu J, Liang W, Zheng J, Wei K. 2011. The assaying of saikosaponins and saikosaponin D in the extracts of Radix Bupleuri. Chin J Trad Med Sci Technol. 18:46–47.
  • Huang W, Lv Z, Sun R. 2013. Research development on chemincal compositions in Bupleurum chinense related with efficacy and toxicity. Chin J Pharmacovigil. 10:545–548.
  • Huang W, Sun R, Zhang Z. 2010. ‘Dose-time-toxicity’ relationship study on hepatotoxicity caused by multiple dose of total Bupleurum saponin crude extracts to rats. Zhongguo Zhong Yao Za Zhi. 35:3344–3347.
  • Ikegami F, Sumino M, Fujii Y, Akiba T, Satoh T. 2006. Pharmacology and toxicology of Bupleurum root-containing Kampo medicines in clinical use. Hum Exp Toxicol. 25:481–494.
  • Jang MJ, Kim YS, Bae EY, Oh TS, Choi HJ, Lee JH, Oh HM, Lee SW. 2014. Saikosaponin D isolated from Bupleurum falcatum inhibits selectin-mediated cell adhesion. Molecules. 19:20340–20349.
  • Japanese Pharmacopoeia Editorial Board. 2011. The Japanese pharmacopoeia. Tokyo, Japan: Japanese Ministry of Health.
  • Jin X, Zhang Y, Li Q, Zhao J. 2013. Mechanisms underlying the beneficial effects of Kaiyu Granule for depression. Neural Regen Res. 8:3241–3248.
  • Judd W. 2008. Plant systematics: a phylogenetic approach, 3rd ed. Sunderland, MA: Sinauer Associates.
  • Kang SJ, Lee YJ, Kim BM, Kim YJ, Woo HD, Jeon HK, Chung HW. 2008. Effect of Bupleuri Radix extracts on the toxicity of 5-fluorouracil in HepG2 hepatoma cells and normal human lymphocytes. Basic Clin Pharmacol Toxicol. 103:305–313.
  • Kim KH, Gam CO, Choi SH, Ku SK. 2012a. Mouse single oral dose toxicity test of bupleuri radix aqueous extracts. Toxicol Res. 28:11–18.
  • Kim SM, Kim SC, Chung IK, Cheon WH, Ku SK. 2012b. Antioxidant and protective effects of Bupleurum falcatum on the l-thyroxine-induced hyperthyroidism in rats. Evid Based Complement Alternat Med. 2012:578497.
  • Kim SO, Park JY, Jeon SY, Yang CH, Kim MR. 2015. Saikosaponin A, an active compound of Radix Bupleuri, attenuates inflammation in hypertrophied 3T3-L1 adipocytes via ERK/NF-κB signaling pathways. Int J Mol Med. 35:1126–1132.
  • Kodama Y, Xiaochuan L, Tsuchiya C, Ohizumi Y, Yoshida M, Nakahata N. 2003. Dual effect of saikogenin d: in vitro inhibition of prostaglandin E2 production and elevation of intracellular free Ca2+ concentration in C6 rat glioma cells. Planta Med. 69:765–767.
  • Kwon S, Lee B, Kim M, Lee H, Park HJ, Hahm DH. 2010. Antidepressant-like effect of the methanolic extract from Bupleurum falcatum in the tail suspension test. Prog Neuropsychopharmacol Biol Psychiatry. 34:265–270.
  • Lee B, Shim I, Lee H, Hahm DH. 2009. Effect of Bupleurum falcatum on the stress-induced impairment of spatial working memory in rats. Biol Pharm Bull. 32:1392–1398.
  • Lee B, Yun HY, Shim I, Lee H, Hahm DH. 2012a. Bupleurum falcatum prevents depression and anxiety-like behaviors in rats exposed to repeated restraint stress. J Microbiol Biotechnol. 22:422–430.
  • Lee JY, Kim HS, Oh TH, Yune TY. 2010. Ethanol extract of Bupleurum falcatum improves functional recovery by inhibiting matrix metalloproteinases-2 and -9 activation and inflammation after spinal cord injury. Exp Neurobiol. 19:146–154.
  • Lee TF, Lin YL, Huang YT. 2011. Kaerophyllin inhibits hepatic stellate cell activation by apoptotic bodies from hepatocytes. Liver Int. 31:618–629.
  • Lee TF, Lin YL, Huang YT. 2012b. Protective effects of kaerophyllin against liver fibrogenesis in rats. Eur J Clin Invest. 42:607–616.
  • Lee TH, Chang J, Kim BM. 2014. Saikosaponin c inhibits lipopolysaccharide-induced apoptosis by suppressing caspase-3 activation and subsequent degradation of focal adhesion kinase in human umbilical vein endothelial cells. Biochem Biophys Res Commun. 445:615–621.
  • Li C, Liu Y, Liu Y, Zhang S, Li P, Shi X, Xu D, Liu T. 2014a. Advances in research of chemical constituents and active constituents of Bupleurum chinense DC. Chinese Arch Trad Chinese Med. 32:2674–2677.
  • Li ZY, Guo Z, Liu YM, Liu XM, Chang Q, Liao YH, Pan RL. 2013. Neuroprotective effects of total saikosaponins of Bupleurum yinchowense on corticosterone-induced apoptosis in PC12 cells. J Ethnopharmacol. 148:794–803.
  • Li ZY, Jiang YM, Liu YM, Guo Z, Shen SN, Liu XM, Pan RL. 2014b. Saikosaponin D acts against corticosterone-induced apoptosis via regulation of mitochondrial GR translocation and a GR-dependent pathway. Prog Neuropsychopharmacol Biol Psychiatry. 53:80–89.
  • Liang Z, Oh K, Wang Y, Yi T, Chen H, Zhao Z. 2014. Cell type-specific qualitative and quantitative analysis of saikosaponins in three Bupleurum species using laser microdissection and liquid chromatography-quadrupole/time of flight-mass spectrometry. J Pharm Biomed Anal. 97:157–165.
  • Lin LT, Chung CY, Hsu WC, Chang SP, Hung TC, Shields J, Russell RS, Lin CC, Li CF, Yen MH, et al. 2014. Saikosaponin B2 is a naturally occurring terpenoid that efficiently inhibits hepatitis C virus entry. J Hepatol. 62:541–548.
  • Lin TY, Chiou CY, Chiou SJ. 2013. Putative genes involved in saikosaponin biosynthesis in Bupleurum species. Int J Mol Sci. 14:12806–12826.
  • Lin X, Wu S, Wang Q, Shi Y, Liu G, Zhi J, Wang F. 2016. Saikosaponin D reduces HO-induced PC12 cell apoptosis by removing ROS and blocking MAPK-dependent oxidative damage. Cell Mol Neurobiol. 36:1365–1375.
  • Liu A, Tanaka N, Sun L, Guo B, Kim JH, Krausz KW, Fang Z, Jiang C, Yang J, Gonzalez FJ. 2014a. Saikosaponin D protects against acetaminophen-induced hepatotoxicity by inhibiting NF-κB and STAT3 signaling. Chem Biol Interact. 223:80–86.
  • Liu K, Lota ML, Casanova J, Tomi F. 2009. The essential oil of Bupleurum fruticosum L. from Corsica: a comprehensive study. Chem Biodivers. 6:2244–2254.
  • Liu Q, Tan L, Bai Y, Liang H, Zhao Y. 2002. The research of genus Bupleurum saponins nearly 10 years. China J Chinese Materia Med. 27:7–11.
  • Liu RY, Li JP. 2014. Saikosaponin-d inhibits proliferation of human undifferentiated thyroid carcinoma cells through induction of apoptosis and cell cycle arrest. Eur Rev Med Pharmacol Sci. 18:2435–2443.
  • Liu Y, Cao C, Ding H. 2014b. Pharmacological experimental study of the anti-depressant effect of total saikosaponins. Afr J Tradit Complement Altern Med. 11:280–284.
  • Liu Y, Liu X, Pan R. 2012. The research of Radix bupleuri toxic effect. Chinese Tradit Patent Med. 34:1148–1151.
  • Lu CN, Yuan ZG, Zhang XL, Yan R, Zhao YQ, Liao M, Chen JX. 2012a. Saikosaponin A and its epimer saikosaponin D exhibit anti-inflammatory activity by suppressing activation of NF-κB signaling pathway. Int Immunopharmacol. 14:121–126.
  • Lu XL, He SX, Ren MD, Wang YL, Zhang YX, Liu EQ. 2012b. Chemopreventive effect of saikosaponin-d on diethylinitrosamine-induced hepatocarcinogenesis: involvement of CCAAT/enhancer binding protein β and cyclooxygenase-2. Mol Med Rep. 5:637–644.
  • Lv L, Huang W, Yu X, Ren H, Sun R. 2009. Comparative research of different Bupleurum chinense composition to influence of hepatotoxicity of rats and oxidative damage mechanism. Zhongguo Zhong Yao Za Zhi. 34:2364–2368.
  • Ma X, Dang C, Kang H, Dai Z, Lin S, Guan H, Liu X, Wang X, Hui W. 2015. Saikosaponin-D reduces cisplatin-induced nephrotoxicity by repressing ROS-mediated activation of MAPK and NF-κB signalling pathways. Int Immunopharmacol. 28:399–408.
  • Mabberley D. 2008. Mabberley’s plant-book: a portable dictionary of plants, their classification and uses. New York, USA: Cambridge University Press.
  • Maccioni P, Lorrai I, Carai MA, Riva A, Morazzoni P, Mugnaini C, Corelli F, Gessa GL, Colombo G. 2016. Reducing effect of saikosaponin a, an active ingredient of Bupleurum falcatum, on alcohol self-administration in rats: possible involvement of the GABA receptor. Neurosci Lett. 621:62–67.
  • Mao X, Miao G, Tao X, Hao S, Zhang H, Li H, Hou Z, Tian R, Lu T, Ma J, et al. 2016. Saikosaponin a protects TBI rats after controlled cortical impact and the underlying mechanism. Am J Transl Res. 8:133–141.
  • Mohammadi A, Nazari H, Imani S, Amrollahi H. 2014. Antifungal activities and chemical composition of some medicinal plants. J Mycol Med. 24:e1–e8.
  • Nakahara Y, Okawa M, Kinjo J, Nohara T. 2011. Oleanene glycosides of the aerial parts and seeds of Bupleurum falcatum and the aerial parts of Bupleurum rotundifolium, and their evaluation as anti-hepatitis agents. Chem Pharm Bull (Tokyo). 59:1329–1339.
  • National Pharmacopoeia Committee. 2010. Pharmacopoeia of People’s Republic of China. Part 1. Beijing, China: Chemical Industry Press.
  • Ou JP, Lin HY, Su KY, Yu SL, Tseng IH, Chen CJ, Hsu HC, Chan DC, Sophia CY. 2012. Potential therapeutic role of Z-isochaihulactone in lung cancer through induction of apoptosis via notch signaling. Evid Based Complement Alternat Med. 2012:809204
  • Pan S. 2006. Bupleurum species: scientific evaluation and clinical applications. Boca Raton, UK: CRC/Taylor & Francis.
  • Pistelli L, Bilia AR, Marsili A, De Tommasi N, Manunta A. 1993. Triterpenoid saponins from Bupleurum fruticosum. J Nat Prod. 56:240–244.
  • Sen L. 1959. Shennong's herbal. Shanghai, China: Shanghai Technology and Health Press.
  • Shin JE, Kim HJ, Kim KR, Lee SK, Park J, Kim H, Park KK, Chung WY. 2015. Type I saikosaponins A and D inhibit osteoclastogenesis in bone marrow-derived macrophages and osteolytic activity of metastatic breast cancer cells. Evid Based Complement Alternat Med. 2015:582437.
  • Sun R, Yang Q. 2011. Experimental study on the ‘dosage-time-toxicity’ relationship of chronic hepatotoxicity inducedby volatile oil from Bupleurum chinense in rats. Pharmacol Clin Chinese Materia Med. 7:49–51.
  • Sun Y, Cai TT, Zhou XB, Xu Q. 2009. Saikosaponin A inhibits the proliferation and activation of T cells through cell cycle arrest and induction of apoptosis. Int Immunopharmacol. 9:978–983.
  • The Inner Mongolia Autonomous Region Health Department. 1988. Chinese medicinal materials standard of Inner Mongolia Autonomous Region. Hohhot, China: Inner Mongolia Medical Press.
  • Tong H, Tian D, He Z, Liu Y, Chu X, Sun X. 2013. Polysaccharides from Bupleurum chinense impact the recruitment and migration of neutrophils by blocking fMLP chemoattractant receptor-mediated functions. Carbohydr Polym. 92:1071–1077.
  • Tsai YJ, Chen IL, Horng LY, Wu RT. 2002. Induction of differentiation in rat C6 glioma cells with saikosaponins. Phytother Res. 16:117–121.
  • Van WB, Wink M. 2004. Medicinal plants of the world: an illustrated scientific guide to important medicinal plants and their uses. Portland, USA: Timber Press.
  • Wang BF, Lin S, Bai MH, Song LQ, Min WL, Wang M, Yang P, Ma HB, Wang XJ. 2014a. Effects of SSd combined with radiation on inhibiting SMMC-7721 hepatoma cell growth. Med Sci Monit. 20:1340–1344.
  • Wang BF, Wang XJ, Kang HF, Bai MH, Guan HT, Wang ZW, Zan Y, Song LQ, Min WL, Lin S, et al. 2014b. Saikosaponin D enhances radiosensitivity of hepatoma cells under hypoxic conditions by inhibiting hypoxia-inducible factor-1α. Cell Physiol Biochem. 33:37–51.
  • Wang C, Zhang T, Cui X, Li S, Zhao X, Zhong X. 2013a. Hepatoprotective effects of a Chinese herbal formula, longyin decoction, on carbon-tetrachloride-induced liver injury in chickens. Evid Based Complement Alternat Med. 2013:392743
  • Wang HW, Liu M, Zhong TD, Fang XM. 2015. Saikosaponin-d attenuates ventilator-induced lung injury in rats. Int J Clin Exp Med. 8:15137–15145.
  • Wang L, Chen Y. 2003. Study on preparation procedure of suppositoria Radix Bupleuri for kids. Zhong Yao Cai. 26:512–514.
  • Wang P, Ren J, Tang J, Zhang D, Li B, Li Y. 2010a. Estrogen-like activities of saikosaponin-d in vitro: a pilot study. Eur J Pharmacol. 626:159–165.
  • Wang Q, Zheng XL, Yang L, Shi F, Gao LB, Zhong YJ, Sun H, He F, Lin Y, Wang X. 2010b. Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells. J Exp Clin Cancer Res. 29:159.
  • Wang X, Wang Q, Burczynski FJ, Kong W, Gong Y. 2013b. Saikosaponin A of Bupleurum chinense (Chaihu) elevates bone morphogenetic protein 4 (BMP-4) during hepatic stellate cell activation. Phytomedicine. 20:1330–1335.
  • Wen S, Huifu X, Hao H. 2011. In vitro anti-influenza A H1N1 effect of extract of Bupleuri Radix. Immunopharmacol Immunotoxicol. 33:433–437.
  • Wong VK, Li T, Law BY, Ma ED, Yip NC, Michelangeli F, Law CK, Zhang MM, Lam KY, Chan PL, et al. 2013a. Saikosaponin-d, a novel SERCA inhibitor, induces autophagic cell death in apoptosis-defective cells. Cell Death Dis. 4:e720
  • Wong VK, Zhang MM, Zhou H, Lam KY, Chan PL, Law CK, Yue PY, Liu L. 2013b. Saikosaponin-d enhances the anticancer potency of TNF-α via overcoming its undesirable response of activating NF-Kappa B signalling in cancer cells. Evid Based Complement Alternat Med. 2013:745295
  • Wong VK, Zhou H, Cheung SS, Li T, Liu L. 2009. Mechanistic study of saikosaponin-d (Ssd) on suppression of murine T lymphocyte activation. J Cell Biochem. 107:303–315.
  • World Health Organization. 1997. Medicinal plants in China: a selection of 150 commonly used species. Manila, Philippines: World Health Organization: Regional Office for the Western Pacific.
  • World Health Organization. 1998. Medicinal plants in the Republic of Korea: information on 150 commonly used medicinal plants. Manila, Philippines: World Health Organization: Regional Office for the Western Pacific.
  • World Health Organization. 1999. WHO Monographs on selected medicinal plants. Geneva, Switzerland: World Health Organization
  • Wu J, Zhang YY, Guo L, Li H, Chen DF. 2013. Bupleurum polysaccharides attenuates lipopolysaccharide-induced inflammation via modulating toll-like receptor 4 signaling. PLoS One. 8:e78051.
  • Wu SJ, Lin YH, Chu CC, Tsai YH, Chao JC. 2008. Curcumin or saikosaponin A improves hepatic antioxidant capacity and protects against CCl4-induced liver injury in rats. J Med Food. 11:224–229.
  • Wu SJ, Tam KW, Tsai YH, Chang CC, Chao JC. 2010. Curcumin and saikosaponin A inhibit chemical-induced liver inflammation and fibrosis in rats. Am J Chin Med. 38:99–111.
  • Wu SX, Sun HF, Yang XH, Long HZ, Ye ZG, Ji SL, Zhang L. 2014. ‘Re-evaluation upon suspected event’ is an approach for post-marketing clinical study: lessons from adverse drug events related to Bupleuri Radix preparations. Zhongguo Zhong Yao Za Zhi. 39:2983–2988.
  • Xie H, Huo KK, Chao Z, Pan SL. 2009. Identification of crude drugs from Chinese medicinal plants of the genus Bupleurum using ribosomal DNA ITS sequences. Planta Med. 75:89–93.
  • Xie JY, Di HY, Li H, Cheng XQ, Zhang YY, Chen DF. 2012. Bupleurum chinense DC polysaccharides attenuates lipopolysaccharide-induced acute lung injury in mice. Phytomedicine 19:130–137.
  • Xie W, Bao Y, Yu LJ, Chen YY. 2006. Effect of saikosaponins on glial fibrillary acidic protein expression in hippocampal astrocytes of pentetrazole-induced chronic kindling rats. Nan Fang Yi Ke Da Xue Xue Bao. 26:452–455.
  • Xie W, Yu YH, Du YP, Zhao YY, Li CZ, Yu L, Duan JH, Xing JL. 2013. Saikosaponin A enhances transient inactivating potassium current in rat hippocampal CA1 neurons. Evid Based Complement Alternat Med. 2013:413092
  • Xue B, Zuguang Y, Baoqiang D, Qing Y, Yongqing X, Xiaohong L, Zelin L. 1996. Antimalarial activities of xiaochaihu tang and its combination with artemisinin in mice infected with Plasmodium berghei. Chinese J Exper Trad Med Formulae. 2:7–10.
  • Yan J, Wei YF, Gu R. 2014. Study on composition of essential oil in above-ground and root of Bupleurum malconense and root of B. chinense by AMDIS and retention index. Zhongguo Zhong Yao Za Zhi. 39:1048–1053.
  • Yao M, Yang J, Cao L, Zhang L, Qu S, Gao H. 2014. Saikosaponin-d inhibits proliferation of DU145 human prostate cancer cells by inducing apoptosis and arresting the cell cycle at G0/G1 phase. Mol Med Rep. 10:365–372.
  • Ying ZL, Li XJ, Dang H, Wang F, Xu XY. 2014. Saikosaponin-d affects the differentiation, maturation and function of monocyte-derived dendritic cells. Exp Ther Med. 7:1354–1358.
  • Yoon SS, Kim HS, Cho HY, Yun J, Chung EY, Jang CG, Kim KJ, Yang CH. 2012. Effect of saikosaponin A on maintenance of intravenous morphine self-administration. Neurosci Lett. 529:97–101.
  • Yoon SS, Seo JW, Ann SH, Kim HY, Kim HS, Cho HY, Yun J, Chung EY, Koo JS, Yang CH. 2013. Effects of saikosaponin A on cocaine self-administration in rats. Neurosci Lett. 555:198–202.
  • You YJ, Lee IS, Kim Y, Bae KH, Ahn BZ. 2002. Antiangiogenic activity of Bupleurum longiradiatum on human umbilical venous endothelial cells. Arch Pharm Res. 25:640–642.
  • Yu YH, Xie W, Bao Y, Li HM, Hu SJ, Xing JL. 2012. Saikosaponin A mediates the anticonvulsant properties in the HNC models of AE and SE by inhibiting NMDA receptor current and persistent sodium current. PLoS One. 7:e50694
  • Zhang BZ, Guo XT, Chen JW, Zhao Y, Cong X, Jiang ZL, Cao RF, Cui K, Gao SS, Tian WR. 2014. Saikosaponin-D attenuates heat stress-induced oxidative damage in LLC-PK1 cells by increasing the expression of anti-oxidant enzymes and HSP72. Am J Chin Med. 42:1261–1277.
  • Zhang F, Chen L, Jin H, Shao J, Wu L, Lu Y, Zheng S. 2015. Activation of Fas death receptor pathway and Bid in hepatocytes is involved in saikosaponin D induction of hepatotoxicity. Environ Toxicol Pharmacol. 41:8–13.
  • Zhao H, Li S, Zhang H, Wang G, Xu G, Zhang H. 2015a. Saikosaponin a protects against experimental sepsis via inhibition of NOD2-mediated NF-κB activation. Exp Ther Med. 10:823–827.
  • Zhao J, Guo Y, Meng X. 1987. The toxic principles of Bupleurum longiradiatum. Acta Pharm Sin. 22:507–511.
  • Zhao L, Zhang H, Bao J, Liu J, Ji Z. 2015b. Saikosaponin-d protects renal tubular epithelial cell against high glucose induced injury through modulation of SIRT3. Int J Clin Exp Med. 8:6472–6481.
  • Zhao W, Li JJ, Yue SQ, Zhang LY, Dou KF. 2012. Antioxidant activity and hepatoprotective effect of a polysaccharide from Bei Chaihu (Bupleurum chinense DC). Carbohydr Polym. 89:448–452.
  • Zhou C, Liu W, He W, Wang H, Chen Q, Song H. 2015. Saikosaponin a inhibits RANKL-induced osteoclastogenesis by suppressing NF-κB and MAPK pathways. Int Immunopharmacol. 25:49–54.
  • Zhou H. 2003. A brief history of Bupleuri Radix research. Harbin, China: Heilongjiang University of Chinese Medicine Press.
  • Zhou X, Cheng H, Xu D, Yin Q, Cheng L, Wang L, Song S, Zhang M. 2014. Attenuation of neuropathic pain by saikosaponin a in a rat model of chronic constriction injury. Neurochem Res. 39:2136–2142.
  • Zhu J, Luo C, Wang P, He Q, Zhou J, Peng H. 2013. Saikosaponin a mediates the inflammatory response by inhibiting the MAPK and NF-κB pathways in LPS-stimulated RAW 264.7 cells. Exp Ther Med. 5:1345–1350.
  • Zuo ZP, Wang ZB, Gao Y, Guo YD, Wang BS, Su B, Song CC. 2013. Bioactivity assay of Bupleurum injection for inhibiting PGE2 release in vitro. Zhongguo Zhong Yao Za Zhi. 38:3957–3960.