CD69 expression as an index of T-cell function: assay standardization, validation and use in monitoring immune recovery

References

• Small TN, Papadopoulos EB, Boulad F, et al. Comparison of immune reconstitution after unrelated and related T-cell-depleted bone marrow transplantation: effect of patient age and donor leukocyte infusions. Blood 1999; 93: 467–80
   PubMed Web of Science ®Google Scholar
• Ault KA, Antin JH, Ginsburg D, et al. Phenotype of recovering lymphoid cell populations after marrow transplantation. J Exp Med 1985; 161: 1483–502
   PubMed Web of Science ®Google Scholar
• Mackall CL, Granger L, Sheard MA, et al. T-cell regeneration after bone marrow transplantation: differential CD45 isoform expression on thymic-derived versus thymic-independent progeny. Blood 1993; 82: 2585–94
   PubMed Web of Science ®Google Scholar
• Heitger A, Neu N, Kern H, et al. Essential role of the thymus to reconstitute naive (CD45RA+) T-helper cells after human allogeneic bone marrow transplantation. Blood 1997; 90: 850–7
   PubMed Web of Science ®Google Scholar
• Kook H, Goldman F, Padley D, et al. Reconstruction of the immune system after unrelated or partially matched T-cell-depleted bone marrow transplantation in children: immunophenotypic analysis and factors affecting the speed of recovery. Blood 1996; 88: 1089–97
   PubMed Web of Science ®Google Scholar
• Mackall CL, Fleisher TA, Brown MR, et al. Age, thymopoiesis, and CD4+ T-lymphocyte regeneration after intensive chemotherapy. N Engl J Med 1995; 332: 143–9
   PubMed Web of Science ®Google Scholar
• Janossy G, Greaves MF, Doenhoff MJ, Snajdr J. Lymphocyte activation. V. Quantitation of the proliferative responses to mitogens using defined T and B cell populations. Clin Exp Immunol 1973; 14: 581–96
   PubMedGoogle Scholar
• Arnaiz-Villena A, Timon M, Rodriguez-Gallego C, et al. Human T-cell activation deficiencies. Immunol Today 1992; 13: 259–65
   Google Scholar
• Testi R, D'Ambrosio D, De Maria R, Santoni A. The CD69 receptor: a multipurpose cell-surface trigger for hematopoietic cells. Immunol Today 1994; 15: 479–83
   PubMedGoogle Scholar
• Caruso A, Licenziati S, Corulli M, et al. Flow cytometric analysis of activation markers on stimulated T cells and their correlation with cell proliferation. Cytometry 1997; 27: 71–6
   PubMedGoogle Scholar
• Craston R, Koh M, McDermott A, et al. Temporal dynamics of CD69 expression on lymphoid cells. J Immunol Methods 1997; 209: 37–45
   PubMed Web of Science ®Google Scholar
• Lowdell MW, Ray N, Craston R, et al. The in vitro detection of anti-leukaemia-specific cytotoxicity after autologous bone marrow transplantation for acute leukaemia. Bone Marrow Transplant 1997; 19: 891–7
   PubMed Web of Science ®Google Scholar
• Mardiney M, Brown MR, Fleisher TA. Measurement of T-cell CD69 expression: a rapid and efficient means to assess mitogen- or antigen-induced proliferative capacity in normals. Cytometry 1996; 26: 305–10
   PubMedGoogle Scholar
• Simms PE, Ellis TM. Utility of flow cytometric detection of CD69 expression as a rapid method for determining poly- and oligoclonal lymphocyte activation. Clin Diagn Lab Immunol 1996; 3: 301–4
   PubMedGoogle Scholar
• Maino VC, Suni MA, Ruitenberg JJ. Rapid flow cytometric method for measuring lymphocyte subset activation. Cytometry 1995; 20: 127–33
   PubMedGoogle Scholar
• Perfetto SP, Hickey TE, Blair PJ, et al. Measurement of CD69 induction in the assessment of immune function in asymptomatic HIV-infected individuals. Cytometry 1997; 30: 1–9
   Google Scholar
• Davis KA, Abrams B, Iyer SB, et al. Determination of CD4 antigen density on cells: role of antibody valency, avidity, clones, and conjugation. Cytometry 1998; 33: 197–205
   PubMedGoogle Scholar
• Lamb LS, Jr, Gee AP, Henslee-Downey PJ, et al. Phenotypic and functional reconstitution of peripheral blood lymphocytes following T cell-depleted bone marrow transplantation from partially mismatched related donors. Bone Marrow Transplant 1998; 21: 461–71
   PubMedGoogle Scholar
• Corradin G, Etlinger HM, Chiller JM. Lymphocyte specificity to protein antigens. I. Characterization of the antigen-induced in vitro T cell-dependent proliferative response with lymph node cells from primed mice. J Immunol 1977; 119: 1048–53
   PubMed Web of Science ®Google Scholar
• Pannu KK, Joe ET, Iyer SB. Performance evaluation of QuantiBRITE phycoerythrin beads. Cytometry 2001; 45: 250–8
   PubMedGoogle Scholar
• Hutchinson P, Divola LA, Holdsworth SR. Mitogen-induced T-cell CD69 expression is a less sensitive measure of T-cell function than [(3)H]-thymidine uptake. Cytometry 1999; 38: 244–9
   PubMedGoogle Scholar
• Lim LC, Fiordalisi MN, Mantell JL, et al. A whole-blood assay for qualitative and semiquantitative measurements of CD69 surface expression on CD4 and CD8 T lymphocytes using flow cytometry. Clin Diagn Lab Immunol 1998; 5: 392–8
   PubMedGoogle Scholar
• Rutella S, Rumi C, Lucia MB, et al. Induction of CD69 antigen on normal CD4+ and CD8+ lymphocyte subsets and its relationship with the phenotype of responding T-cells. Cytometry 1999; 38: 95–101
   Google Scholar
• Mehta BA, Maino VC. Simultaneous detection of DNA synthesis and cytokine production in staphylococcal enterotoxin B activated CD4+ T lymphocytes by flow cytometry. J Immunol Methods 1997; 208: 49–59
   PubMed Web of Science ®Google Scholar
• Balboa MA, Izquierdo M, Sanchez-Madrid F, et al. Analysis of different protein kinase C-dependent events in T cells from allogeneic bone marrow transplantation recipients. Clin Exp Immunol 1992; 87: 478–84
   Google Scholar
• Gibbons DC, Evans TG. CD69 expression after antigenic stimulation. Cytometry 1996; 23: 260–1
   Google Scholar
• Krowka JF, Cuevas B, Maron DC, et al. Expression of CD69 after in vitro stimulation: a rapid method for quantitating impaired lymphocyte responses in HIV-infected individuals. J Acquir Immune Defic Syndr Hum Retrovirol 1996; 11: 95–104
   PubMedGoogle Scholar