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Original Research

Highly sensitive droplet digital PCR-based diagnostics for the surveillance of malaria vector populations in low transmission and incipient resistance settings

ORCID Icon, ORCID Icon & ORCID Icon
Pages 1105-1114 | Received 24 Mar 2021, Accepted 28 Jul 2021, Published online: 09 Aug 2021

References

  • WHO. World Health Organization: World malaria report 2020. [cited 2021 Jun 10]. Available from: https://www.who.int/publications/i/item/9789240015791
  • Miller LH, Ackerman HC, Su XZ, et al. Malaria biology and disease pathogenesis: insights for new treatments. Nat Med. 2013;19(2):156–167.
  • de Koning-Ward TF, Gilson PR, Crabb BS. Advances in molecular genetic systems in malaria. Nat Rev Microbiol. 2015;13(6):373–387.
  • Ghosh AK, Jacobs-Lorena M. Plasmodium sporozoite invasion of the mosquito salivary gland. Curr Opin Microbiol. 2009;12(4):394–400.
  • Kefi M, Mavridis K, Simoes ML, et al. New rapid one-step PCR diagnostic assay for Plasmodium falciparum infective mosquitoes. Sci Rep. 2018;8(1):1462.
  • Hemingway J. The role of vector control in stopping the transmission of malaria: threats and opportunities. Philos Trans R Soc Lond B Biol Sci. 2014;369(1645):20130431.
  • Tanner M, Greenwood B, Whitty CJ, et al. Malaria eradication and elimination: views on how to translate a vision into reality. BMC Med. 2015;13(1):167.
  • Nkumama IN, O’Meara WP, Osier FHA. Changes in malaria epidemiology in Africa and new challenges for elimination. Trends Parasitol. 2017;33(2):128–140.
  • Alout H, Roche B, Dabire RK, et al. Consequences of insecticide resistance on malaria transmission. PLoS Pathog. 2017;13(9):e1006499.
  • Ranson H, N’Guessan R, Lines J, et al. Pyrethroid resistance in African anopheline mosquitoes: what are the implications for malaria control? Trends Parasitol. 2011;27(2):91–98.
  • Oxborough RM, Seyoum A, Yihdego Y, et al. Susceptibility testing of Anopheles malaria vectors with the neonicotinoid insecticide clothianidin; results from 16 African countries, in preparation for indoor residual spraying with new insecticide formulations. Malar J. 2019;18(1):264.
  • Ngufor C, Agbevo A, Fagbohoun J, et al. Efficacy of royal guard, a new alpha-cypermethrin and pyriproxyfen treated mosquito net, against pyrethroid-resistant malaria vectors. Sci Rep. 2020;10(1):12227.
  • Ngufor C, Fongnikin A, Hobbs N, et al. Indoor spraying with chlorfenapyr (a pyrrole insecticide) provides residual control of pyrethroid-resistant malaria vectors in southern Benin. Malar J. 2020;19(1):249.
  • Hemingway J, Shretta R, Wells TN, et al. Tools and strategies for malaria control and elimination: what do we need to achieve a grand convergence in malaria? PLoS Biol. 2016;14(3):e1002380.
  • Killeen GF. Characterizing, controlling and eliminating residual malaria transmission. Malar J. 2014;13(1):330.
  • Fontenille D, Meunier JY, Nkondjio CA, et al. Use of circumsporozoite protein enzyme-linked immunosorbent assay compared with microscopic examination of salivary glands for calculation of malaria infectivity rates in mosquitoes (Diptera: culicidae) from Cameroon. J Med Entomol. 2001;38(3):451–454.
  • Smith DL, Battle KE, Hay SI, et al. Ross, macdonald, and a theory for the dynamics and control of mosquito-transmitted pathogens. PLoS Pathog. 2012;8(4):e1002588.
  • Snounou G, Nested SB. PCR analysis of plasmodium parasites. Methods Mol Med. 2002;72:189–203.
  • Bagi J, Grisales N, Corkill R, et al. When a discriminating dose assay is not enough: measuring the intensity of insecticide resistance in malaria vectors. Malar J. 2015;14(1):210.
  • Vontas J, Mavridis K. Vector population monitoring tools for insecticide resistance management: myth or fact? Pestic Biochem Physiol. 2019;161:54–60.
  • Grigoraki L, Puggioli A, Mavridis K, et al. Striking diflubenzuron resistance in Culex pipiens, the prime vector of West Nile Virus. Sci Rep. 2017;7(1):11699.
  • Balabanidou V, Kampouraki A, MacLean M, et al. Cytochrome P450 associated with insecticide resistance catalyzes cuticular hydrocarbon production in Anopheles gambiae. Proc Natl Acad Sci U S A. 2016;113(33):9268–9273.
  • Hanley JA, McNeil BJ. The meaning and use of the area under a receiver operating characteristic (ROC) curve. Radiology. 1982;143(1):29–36.
  • Lin LI. A concordance correlation coefficient to evaluate reproducibility. Biometrics. 1989;45(1):255–268.
  • Bland JM, Altman DG. Measuring agreement in method comparison studies. Stat Methods Med Res. 1999;8(2):135–160.
  • Bass C, Nikou D, Blagborough AM, et al. PCR-based detection of Plasmodium in Anopheles mosquitoes: a comparison of a new high-throughput assay with existing methods. Malar J. 2008;7(1):177.
  • Lynd A, Oruni A, Van’t Hof AE, et al. Insecticide resistance in Anopheles gambiae from the northern Democratic Republic of Congo, with extreme knockdown resistance (kdr) mutation frequencies revealed by a new diagnostic assay. Malar J. 2018;17(1):412.
  • Lavebratt C, Sengul S, Jansson M, et al. Pyrosequencing-based SNP allele frequency estimation in DNA pools. Hum Mutat. 2004;23(1):92–97.
  • McBride GB. A proposal for strength-of-agreement criteria for Lin’s concordance correlation coefficient. NIWA Client Report, HAM2005-062 (2005).
  • Abraham M, Massebo F, Lindtjorn B. High entomological inoculation rate of malaria vectors in area of high coverage of interventions in southwest Ethiopia: implication for residual malaria transmission. Parasite Epidemiol Control. 2017;2(2):61–69.
  • Burke A, Dahan-Moss Y, Duncan F, et al. Anopheles parensis contributes to residual malaria transmission in South Africa. Malar J. 2019;18(1):257.
  • Kweka EJ, Nkya WM, Mahande AM, et al. Mosquito abundance, bed net coverage and other factors associated with variations in sporozoite infectivity rates in four villages of rural Tanzania. Malar J. 2008;7(1):59.
  • Kgoroebutswe TK, Ramatlho P, Reeder S, et al. Distribution of Anopheles mosquito species, their vectorial role and profiling of knock-down resistance mutations in Botswana. Parasitol Res. 2020;119(4):1201–1208.
  • Grossenbacher B, Holzschuh A, Hofmann NE, et al. Molecular methods for tracking residual Plasmodium falciparum transmission in a close-to-elimination setting in Zanzibar. Malar J. 2020;19(1):50.
  • Rondon S, Leon C, Link A, et al. Prevalence of Plasmodium parasites in non-human primates and mosquitoes in areas with different degrees of fragmentation in Colombia. Malar J. 2019;18(1):276.
  • Aranda C, Sanchez-Seco MP, Caceres F, et al. Detection and monitoring of mosquito flaviviruses in Spain between 2001 and 2005. Vector Borne Zoonotic Dis. 2009;9(2):171–178.
  • Gu W, Lampman R, Novak RJ. Assessment of arbovirus vector infection rates using variable size pooling. Med Vet Entomol. 2004;18(2):200–204.
  • Gu W, Unnasch TR, Katholi CR, et al. Fundamental issues in mosquito surveillance for arboviral transmission. Trans R Soc Trop Med Hyg. 2008;102(8):817–822.
  • Mavridis K, Fotakis EA, Kioulos I, et al. Detection of West Nile Virus - Lineage 2 in Culex pipiens mosquitoes, associated with disease outbreak in Greece, 2017. Acta Trop. 2018;182:64–68.
  • Vincent GP, Davis JK, Wittry MJ, et al. Epidemic West Nile Virus infection rates and endemic population dynamics among south dakota mosquitoes: a 15-yr study from the United States Northern Great Plains. J Med Entomol. 2020;57(3):862–871.
  • Mavridis K, Wipf N, Muller P, et al. Detection and monitoring of insecticide resistance mutations in Anopheles gambiae: individual vs pooled specimens. Genes (Basel). 2018;9(10):479.
  • Djogbenou LS, Assogba B, Essandoh J, et al. Estimation of allele-specific ace-1 duplication in insecticide-resistant Anopheles mosquitoes from West Africa. Malar J. 2015;14(1):507.
  • Bass C, Nikou D, Vontas J, et al. The Vector Population Monitoring Tool (VPMT): high-throughput DNA-Based diagnostics for the monitoring of mosquito vector populations. Malar Res Treat. 2010;2010:190434.
  • Van Leeuwen T, Dermauw W, Mavridis K, et al. Significance and interpretation of molecular diagnostics for insecticide resistance management of agricultural pests. Curr Opin Insect Sci. 2020;39:69–76.
  • Michaelidou K, Koutoulaki C, Mavridis K, et al. Detection of KRAS G12/G13 mutations in cell free-DNA by droplet digital PCR, offers prognostic information for patients with advanced non-small cell lung cancer. Cells. 2020;9(11):2514.
  • Gerdes L, Iwobi A, Busch U, et al. Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms. Biomol Detect Quantif. 2016;7:9–20.
  • Taylor SC, Laperriere G, Germain H. Droplet digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data. Sci Rep. 2017;7(1):2409.
  • Mitsakakis K, Hin S, Muller P, et al. Converging human and malaria vector diagnostics with data management towards an integrated holistic one health approach. Int J Environ Res Public Health. 2018;15(2):259.
  • Rabinowitz P, Conti L. One Health and emerging infectious diseases: clinical perspectives. Curr Top Microbiol Immunol. 2013;365:17–29.

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