Chlamydia trachomatis: quest for an eye-opening vaccine breakthrough

References

• Witkin SS, Minis E, Athanasiou A, et al. Chlamydia trachomatis: the persistent pathogen. Clin Vaccine Immunol Internet]. 2017;24:e00203–17. Available from. https://pubmed.ncbi.nlm.nih.gov/2883536010.1128/CVI.00203-17
   PubMedGoogle Scholar
• Paavonen J, Eggert-Kruse W. Chlamydia trachomatis: impact on human reproduction. Hum Reprod Update. 1999;5:433–447.
   PubMed Web of Science ®Google Scholar
• Graversen VK, El HS, Gold A, et al. History through the eyes of a pandemic. Curr Opin Ophthalmol. 2020;31:538–548.
   PubMed Web of Science ®Google Scholar
• Sixt BS. Host cell death during infection with Chlamydia: a double-edged sword. FEMS Microbiol Rev. 2021 45 1 ;fuaa043.
   PubMed Web of Science ®Google Scholar
• WHO. Trachoma [Internet]. [cited 2021 Jul 22]. p. Updated on 2021 May 9. Available from: https://www.who.int/news-room/fact-sheets/detail/trachoma.
   Google Scholar
• Moloo A. Neglected tropical diseases: treating more than one billion people for the fifth consecutive year [Internet]. Dep. news WHO. [cited 2021 Jul 22]. p. Updated on: 16 July 2020. Available from: https://www.who.int/news/item/16-07-2020-neglected-tropical-diseases-treating-more-than-one-billion-people-for-the-fifth-consecutive-year#:~:text=Buruli ulcer%3B Chagas disease (American,disease)%3B lymphatic filariasis%3B mycetoma%2C.
   Google Scholar
• Ferede AT, Dadi AF, Tariku A, et al. Prevalence and determinants of active trachoma among preschool-aged children in Dembia District, Northwest Ethiopia. Infect Dis Poverty. 2017;6:128.
   PubMed Web of Science ®Google Scholar
• Burton MJ, Mabey DCW. The global burden of trachoma: a review. PLoS Negl Trop Dis. 2009;3:e460.
   PubMed Web of Science ®Google Scholar
• Brunham RC, Rey-Ladino J. Immunology of Chlamydia infection: implications for a Chlamydia trachomatis vaccine. Nat Rev Immunol. 2005;5:149–161.
   PubMed Web of Science ®Google Scholar
• Elwell C, Mirrashidi K, Engel J Chlamydia cell biology and pathogenesis.Nat Rev Microbiol.Internet]. 2016 April /25. 2016;14:385–400. Available from.https://pubmed.ncbi.nlm.nih.gov/27108705 •• Explains molecular aspects of chlamydial disease
   PubMed Web of Science ®Google Scholar
• Nash AA, Dalziel RG, Fitzgerald JR Mechanisms of Cell and Tissue Damage. Mims’ Pathog Infect Dis [Internet]. 2015 February /06.2015;171–231. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158287/.
   Google Scholar
• Brothwell JA, Brockett M, Banerjee A, et al. Genome copy number regulates inclusion expansion, septation, and infectious developmental form conversion in Chlamydia trachomatis. J Bacteriol. 2021 203 6 ;e00630–20.
   Web of Science ®Google Scholar
• Diab MM, Allen RC, Gawdat TI, et al. Trachoma elimination, approaching 2020. Curr Opin Ophthalmol. 2018;29:451–457.
   PubMed Web of Science ®Google Scholar
• O’Brien KS, Emerson P, Hooper PJ, et al. Antimicrobial resistance following mass azithromycin distribution for trachoma: a systematic review. Lancet Infect Dis. 2019;19:e14–e25.
   PubMed Web of Science ®Google Scholar
• de la Maza Lm, Zhong G, Brunham RC. Update on Chlamydia trachomatis Vaccinology. Clin Vaccine Immunol. 2017 24 4 ;e00543–16.
   PubMedGoogle Scholar
• Pickering H, Chernet A, Sata E, et al. Genomics of Ocular Chlamydia trachomatis after 5 years of SAFE interventions for trachoma in Amhara, Ethiopia. J Infect Dis. 2020 225 6 994–1004 .
   Web of Science ®Google Scholar
• Harding-Esch EM, Holland MJ, Schémann J-F, et al. Facial cleanliness indicators by time of day: results of a cross-sectional trachoma prevalence survey in Senegal. Parasit Vectors. 2020;13:556.
   PubMed Web of Science ®Google Scholar
• Tian L, Wang N-L. Trachoma control: the SAFE strategy. Int J Ophthalmol. 2018;11:1887–1888.
   PubMed Web of Science ®Google Scholar
• Derrick T, h RC, Last AR, et al. Trachoma and ocular chlamydial infection in the era of genomics. Mediators Inflamm. 2015;2015:791847.
   PubMed Web of Science ®Google Scholar
• Hu VH, Holland MJ, Burton MJ. Trachoma: protective and pathogenic ocular immune responses to Chlamydia trachomatis. PLoS Negl Trop Dis. 2013;7:e2020.
   PubMed Web of Science ®Google Scholar
• Ghosh K. Evolution and selection of human leukocyte antigen alleles by Plasmodium falciparum infection. Hum Immunol. 2008;69:856–860.
   PubMed Web of Science ®Google Scholar
• Savy M, Hennig BJ, Doherty CP, et al. Haptoglobin and Sickle Cell Polymorphisms and Risk of Active Trachoma in Gambian Children.PLoS One.Internet]. 2010;5:e11075. Available from;• This article describes genetic polymorphic traits associated with Trachoma
   PubMed Web of Science ®Google Scholar
• Stothard DR, Toth GA, Batteiger BE. Polymorphic membrane protein H has evolved in parallel with the three disease-causing groups of Chlamydia trachomatis. Infect Immun. 2003;71:1200–1208.
   PubMed Web of Science ®Google Scholar
• Carlson JH, Hughes S, Hogan D, et al. Polymorphisms in the chlamydia trachomatis cytotoxin locus associated with ocular and genital isolates. Infect Immun. 2004;72:7063–7072.
   PubMed Web of Science ®Google Scholar
• Tietzel I, El-Haibi C, Carabeo RA. Human guanylate binding proteins potentiate the anti-chlamydia effects of interferon-gamma. PLoS One. 2009;4:e6499.
   PubMed Web of Science ®Google Scholar
• Rockey DD, Heinzen RA, Hackstadt T. Cloning and characterization of a Chlamydia psittaci gene coding for a protein localized in the inclusion membrane of infected cells. Mol Microbiol. 1995;15:617–626.
   PubMed Web of Science ®Google Scholar
• Pennini ME, Perrinet S, Dautry-Varsat A, et al. Histone methylation by NUE, a novel nuclear effector of the intracellular pathogen chlamydia trachomatis. PLoS Pathog. 2010;6:e1000995.
   PubMed Web of Science ®Google Scholar
• Abraham S, Juel HB, Bang P, et al. Safety and immunogenicity of the chlamydia vaccine candidate CTH522 adjuvanted with CAF01 liposomes or aluminium hydroxide: a first-in-human, randomised, double-blind, placebo-controlled, phase 1 trial, Lancet Infect Dis [Internet]. 2019;19:1091–1100. Available from].;:. https://doi.org/10.1016/S1473-3099(19)30279-8. •• A Detailed manuscript providing clinical evaluation study results for a chalmydial vaccine candidate.
   PubMed Web of Science ®Google Scholar
• Staedel C, Darfeuille F. MicroRNAs and bacterial infection. Cell Microbiol. 2013;15:1496–1507.
   PubMed Web of Science ®Google Scholar
• Dzakah EE, Huang L, Xue Y, et al. Host cell response and distinct gene expression profiles at different stages of Chlamydia trachomatis infection reveals stage-specific biomarkers of infection. BMC Microbiol. Internet]. 2021;21:3. Available from https://doi.org/10.1186/s12866-020-02061-6
   PubMed Web of Science ®Google Scholar
• Swenson DL, Wang D, Luo M, et al. Vaccine to confer to nonhuman primates complete protection against multistrain Ebola and Marburg virus infections. Clin Vaccine Immunol. 2008;15:460–467.
   PubMed Web of Science ®Google Scholar
• Schautteet K, De Clercq E, Vanrompay D Chlamydia trachomatis vaccine research through the years. Infect Dis Obstet Gynecol [Internet]. 2011 June /26. 2011;963513. Available from: https://pubmed.ncbi.nlm.nih.gov/21747646.
   Google Scholar
• Hafner L, Beagley K, Timms P. Chlamydia trachomatis infection: host immune responses and potential vaccines. Mucosal Immunol Internet]. 2008;1:116–130. Available from.;:. https://doi.org/10.1038/mi.2007.19
   PubMed Web of Science ®Google Scholar
• Mogensen TH. Pathogen recognition and inflammatory signaling in innate immune defenses. Clin Microbiol Rev. [Internet] 2009;22:240–273. Available from.;:. https://pubmed.ncbi.nlm.nih.gov/19366914
   PubMed Web of Science ®Google Scholar
• Redgrove KA, McLaughlin EA. The role of the immune response in chlamydia trachomatis infection of the male genital tract: a double-edged sword. Front Immunol Internet]. 2014;5:534. Available from.;:. https://pubmed.ncbi.nlm.nih.gov/25386180
   PubMed Web of Science ®Google Scholar
• Lietman TM, Oldenburg CE, Keenan JD. Trachoma: time to Talk Eradication. Ophthalmology Internet]. 2020;127:11–13. Available from.;:. https://doi.org/10.1016/j.ophtha.2019.11.001.
   PubMed Web of Science ®Google Scholar
• CDC. Chlamydia - CDC Fact Sheet (Detailed) [Internet]. Centers Dis. Control Prev. 2021 [cited 2021 Jun 16]. p. Available from 2021 Jan 19: https://www.cdc.gov/std/chlamydia/stdfact-chlamydia-detailed.htm.
   Google Scholar
• Phillips S, Quigley BL, Timms P. Seventy Years of chlamydia vaccine research - limitations of the past and directions for the future Front Microbiol. 2019;10:70.
   PubMed Web of Science ®Google Scholar
• Cheong HC, Lee CYQ, Cheok YY, et al. Chlamydiaceae: diseases in primary hosts and zoonosis. Microorganisms. Internet]. 2019;7:146. Available from.https://pubmed.ncbi.nlm.nih.gov/31137741
   Web of Science ®Google Scholar
• Vasilevsky S, Stojanov M, Greub G, et al. Chlamydial polymorphic membrane proteins: regulation, function and potential vaccine candidates. Virulence. 2016;7:11–22.
   PubMed Web of Science ®Google Scholar
• Poston TB, Gottlieb SL, Darville T. Status of vaccine research and development of vaccines for chlamydia trachomatis infection. Vaccine. 2019;37:7289–7294.
   PubMed Web of Science ®Google Scholar
• De Clercq E, Kalmar I, Vanrompay D. Animal models for studying female genital tract infection with chlamydia trachomatis. Infect Immun. Internet].2013 July /08 2013;81:3060–3067. Available from;:.:https://pubmed.ncbi.nlm.nih.gov/23836817
   PubMed Web of Science ®Google Scholar
• Rajeeve K, Sivadasan R. Transcervical mouse infections with chlamydia trachomatis and determination of bacterial burden. Bio-protocol. 2020;10:e3506.
   PubMed Web of Science ®Google Scholar
• Käser T, Pasternak JA, Delgado-Ortega M, et al. Chlamydia suis and chlamydia trachomatis induce multifunctional CD4 T cells in pigs. Vaccine. Internet]. 2017;35:91–100. Available from https://www.sciencedirect.com/science/article/pii/S0264410X16311124
   PubMed Web of Science ®Google Scholar
• Lorenzen E, Follmann F, Secher JO, et al. Intrauterine inoculation of minipigs with chlamydia trachomatis during diestrus establishes a longer lasting infection compared to vaginal inoculation during estrus. Microbes Infect. Internet]. 2017;19:334–342. Available from.https://www.sciencedirect.com/science/article/pii/S1286457917300254
   PubMed Web of Science ®Google Scholar
• Randall A, Teng A, Liang X, et al. A primary chlamydia trachomatis genital infection of rhesus macaques identifies new immunodominant B-cell antigens. PLoS One. 2021;16:e0250317.
   PubMed Web of Science ®Google Scholar
• Patton DL, Teng A, Randall A, et al. Whole genome identification of C. trachomatis immunodominant antigens after genital tract infections and effect of antibiotic treatment of pigtailed macaques. J Proteomics. 2014;108:99–109.
   PubMed Web of Science ®Google Scholar
• Sukumar P, TD F, Guangming Z, et al. Transcervical inoculation with chlamydia trachomatis induces infertility in HLA-DR4 transgenic and wild-Type mice. Infect Immun. Internet]. 2021;86:e00722–17. Available from https://doi.org/10.1128/IAI.00722-17.
   Google Scholar
• Olsen AW, Lorenzen EK, Rosenkrands I, et al. Protective effect of vaccine promoted neutralizing antibodies against the intracellular pathogen chlamydia trachomatis [Internet]. Front. Immunol. 2017. p. 1652. Available from: https://www.frontiersin.org/article/10.3389/fimmu.2017.01652.• Interesting read for understanding the immunological mechanism after vaccination
   Google Scholar
• Nguyen NDNT, Olsen AW, Lorenzen E, et al. Parenteral vaccination protects against transcervical infection with Chlamydia trachomatis and generate tissue-resident T cells post-challenge. Npj Vaccines. Internet]. 2020;5:7.Available from https://doi.org/10.1038/s41541-020-0157-x.
   PubMed Web of Science ®Google Scholar
• Li M, Shi W, Yang J, et al. Generation of a novel affibody molecule targeting chlamydia trachomatis MOMP. Appl Microbiol Biotechnol. Internet]. 2021;105:1477–1487. Available from https://doi.org/10.1007/s00253-021-11128-x
   PubMed Web of Science ®Google Scholar
• McIntosh EDG. Development of vaccines against the sexually transmitted infections gonorrhoea, syphilis, chlamydia, herpes simplex virus, human immunodeficiency virus and zika virus. Ther Adv vaccines Immunother. Internet]. 2020;8:25151355209238872515135520923887. Available from: 25151355209238872515135520923887. Available from: https://pubmed.ncbi.nlm.nih.gov/32647779
   PubMedGoogle Scholar
• Olivares-Zavaleta N, Whitmire W, Gardner D, et al. Immunization with the attenuated plasmidless Chlamydia trachomatis L2(25667R) strain provides partial protection in a murine model of female genitourinary tract infection. Vaccine [Internet]. 2009 December /08. 2010;28:1454–1462. Available from. https://pubmed.ncbi.nlm.nih.gov/2000426510.1016/j.vaccine.2009.11.073
   PubMed Web of Science ®Google Scholar
• In BYC, Baron S.editor.Medical Microbiology. 4th edition. Galveston (TX): university of texas medical branch at Galveston;.Chapter.1996;39: Available from.https://www.ncbi.nlm.nih.gov/books/NBK8091
   Google Scholar
• Faris R, Andersen SE, McCullough A, et al. Chlamydia trachomatis serovars drive differential production of proinflammatory cytokines and chemokines depending on the type of cell infected [Internet]. Front. Cell. Infect. Microbiol 2019. p. 399. Available from: https://www.frontiersin.org/article/10.3389/fcimb.2019.00399.
   Google Scholar
• Mabey DCW, Hu V, Bailey RL, et al. Towards a safe and effective chlamydial vaccine: lessons from the eye, Vaccine [Internet]. 2014;32:1572–1578. Available from].;:. https://pubmed.ncbi.nlm.nih.gov/24606636. • Interesting read for vaccine development against trachoma.
   PubMed Web of Science ®Google Scholar
• Woolridge RL, Grayston JT, Chang IH, et al. Field trial of a monovalent and of a bivalent mineral oil adjuvant trachoma vaccine in Taiwan school children. Am J Ophthalmol. 1967;63:1645–1650.
   Web of Science ®Google Scholar
• Sowa S, Sowa J, Collier LH, et al. Trachoma vaccine field trials in the gambia. J Hyg (Lond). 1969;67:699–717.
   PubMedGoogle Scholar
• Grayston JT, Wang SP, Yang YF, et al. The effect of trachoma virus vaccine on the course of experimental trachoma infection in blind human volunteers. J Exp Med. 1962;115:1009–1022.
   PubMed Web of Science ®Google Scholar
• Dhir SP, Agarwal LP, Detels R, et al. Field trial of two bivalent trachoma vaccines in children of Punjab Indian villages. Am J Ophthalmol. 1967;63:1639–1644.
   Web of Science ®Google Scholar
• Kari L, Whitmire WM, Crane DD, et al. Chlamydia trachomatis native major outer membrane protein induces partial protection in nonhuman primates: implication for a trachoma transmission-blocking vaccine. J Immunol. 2009;182:8063–8070.
   PubMed Web of Science ®Google Scholar
• Richmond P, Hatchuel L, Dong M Safety and immunogenicity of S-Trimer (SCB-2019), a protein subunit vaccine candidate for COVID-19 in healthy adults: a phase 1, randomised, double-blind, placebo-controlled trial, et al. Lancet. Vol. 397, ; 2021. 682–694.
   PubMed Web of Science ®Google Scholar
• Goodnow CC, Vinuesa CG, Randall KL, et al. Control systems and decision making for antibody production. Nat Immunol. 2010;11:681–688. Internet].;:. Available from.
   PubMed Web of Science ®Google Scholar
• Wang S, Thomas Grayston J, Russell Alexander E. Trachoma vaccine studies in monkeys. Am J Ophthalmol. Internet]. 1967;63:1615–1630. Available from https://www.sciencedirect.com/science/article/pii/0002939467941554
   PubMed Web of Science ®Google Scholar
• Gondek DC, Roan NR, Starnbach MN. T cell responses in the absence of ifn-γ exacerbate uterine infection with <em>chlamydia, trachomatis. J Immunol Internet]. 2009;183:1313 LP 1319. Available from 1319. Available from http://www.jimmunol.org/content/183/2/1313.abstract
   PubMed Web of Science ®Google Scholar
• Badamchi-Zadeh A, McKay PF, Holland MJ, et al. Intramuscular immunisation with chlamydial proteins induces chlamydia trachomatis specific ocular antibodies. PLoS One. 2015;10:e0141209.
   PubMed Web of Science ®Google Scholar
• Nita S, HS E, Besmir H, et al. Chlamydia-Specific iga secretion in the female reproductive tract induced via per-oral immunization confers protection against primary chlamydia challenge, Infect Immun [Internet]. 2021;89:e00413–20. Available from] https://doi.org/10.1128/IAI.00413-20. •• A very good manuscript providing details about mucosal vaccine delivery.
   Web of Science ®Google Scholar
• Zhu C, Lin H, Tang L, et al. Oral Chlamydia vaccination induces transmucosal protection in the airway. Vaccine. Internet]. 2018;36:2061–2068. Available from https://www.sciencedirect.com/science/article/pii/S0264410X18303517
   PubMed Web of Science ®Google Scholar
• Pal S, Tifrea DF, Follmann F, et al. The cationic liposomal adjuvants CAF01 and CAF09 formulated with the major outer membrane protein elicit robust protection in mice against a Chlamydia muridarum respiratory challenge. Vaccine. 2017;35:1705–1711.
   PubMed Web of Science ®Google Scholar
• Pal S, Favaroni A, Tifrea DF, et al. Comparison of the nine polymorphic membrane proteins of Chlamydia trachomatis for their ability to induce protective immune responses in mice against a C. muridarum challenge. Vaccine. 2017;35:2543–2549.
   PubMed Web of Science ®Google Scholar
• Wern JE, Sorensen MR, Olsen AW, et al. Simultaneous subcutaneous and intranasal administration of a caf01-adjuvanted chlamydia vaccine elicits elevated iga and protective th1/th17 responses in the genital tract [Internet]. Front. Immunol. 2017. p. 569. Available from https://www.frontiersin.org/article/10.3389/fimmu.2017.00569.
   Google Scholar
• Jiang P, Cai Y, Chen J, et al. Evaluation of tandem Chlamydia trachomatis MOMP multi-epitopes vaccine in BALB/c mice model. Vaccine Internet]. 2017;35:3096–3103. Available from.;.:https://www.sciencedirect.com/science/article/pii/S0264410X17305091
   PubMed Web of Science ®Google Scholar
• Olsen AW, Rosenkrands I, Holland MJ, et al. A Chlamydia trachomatis VD1-MOMP vaccine elicits cross-neutralizing and protective antibodies against C/C-related complex serovars. Npj Vaccines. 2021;6:58. Internet].Available from.
   PubMed Web of Science ®Google Scholar
• Yu H, Karunakaran KP, Jiang X, et al. Comparison of Chlamydia outer membrane complex to recombinant outer membrane proteins as vaccine. Vaccine Internet]. 2020;38:3280–3291. Available from.;.:https://www.sciencedirect.com/science/article/pii/S0264410X20302826
   PubMed Web of Science ®Google Scholar
• Rose F, Wern JE, Gavins F, et al. A strong adjuvant based on glycol-chitosan-coated lipid-polymer hybrid nanoparticles potentiates mucosal immune responses against the recombinant chlamydia trachomatis fusion antigen CTH522. J Control Release Internet]. 2018;271:88–97. Available from.;.:https://www.sciencedirect.com/science/article/pii/S016836591731060X
   PubMed Web of Science ®Google Scholar
• Kuczkowska K, Myrbråten I, Øverland L, et al. Lactobacillus plantarum producing a chlamydia trachomatis antigen induces a specific IgA response after mucosal booster immunization. PLoS One. Internet]. 2017;12:e0176401. Available from. https://doi.org/10.1371/journal.pone.0176401.
   PubMed Web of Science ®Google Scholar
• Lanfermann C, Wintgens S, Ebensen T, et al. Prophylactic Multi-Subunit vaccine against chlamydia trachomatis: in vivo evaluation in mice. Vaccines. 2021 9 6 609 .
   Web of Science ®Google Scholar
• Ganda IS, Zhong Q, Hali M, et al. Dendrimer-conjugated peptide vaccine enhances clearance of Chlamydia trachomatis genital infection. Int J Pharm. Internet]. 2017;527:79–91. Available from https://www.sciencedirect.com/science/article/pii/S037851731730457X
   PubMed Web of Science ®Google Scholar
• Steven L, Mahony James B. Intranasal vaccination with a chimeric chlamydial antigen bd584 confers protection against chlamydia trachomatis genital tract infection J Vaccines Immunol . . 2020 6 1 : 10–17
   Google Scholar
• Wali S, Gupta R, J-j Y, et al. Chlamydial protease-like activity factor mediated protection against C. trachomatis in guinea pigs. Immunol Cell Biol. 2017;95:454–460. Internet].;:. Available from
   PubMed Web of Science ®Google Scholar
• Pal S, Ausar SF, Tifrea DF, et al. Protection of outbred mice against a vaginal challenge by a chlamydia trachomatis serovar E recombinant major outer membrane protein vaccine is dependent on phosphate substitution in the adjuvant. Hum Vaccin Immunother. Internet]. 2020;16:2537–2547. Available from https://doi.org/10.1080/21645515.2020.1717183
   PubMed Web of Science ®Google Scholar
• Koroleva EA, V KN, Shcherbinin DN, et al. Chlamydial type iii secretion system needle protein induces protective immunity against chlamydia muridarum intravaginal infection. Lopalco L, editor. Biomed Res Int. Internet]. 2017;2017:3865802. Available from https://doi.org/10.1155/2017/3865802
   PubMed Web of Science ®Google Scholar
• Pal S, Mirzakhanyan Y, Gershon P, et al. Induction of protection in mice against a respiratory challenge by a vaccine formulated with exosomes isolated from Chlamydia muridarum infected cells. Npj Vaccines. 2020;5:87. [Internet] Available from
   PubMed Web of Science ®Google Scholar
• Kaufhold RM, Boddicker MA, Field JA, et al. Evaluating potential vaccine antigens in both the chlamydia trachomatis and chlamydia muridarum intravaginal mouse challenge models. World J Vaccines. 2019;9:2160–5815. Print.
   Google Scholar
• Pais R, Omosun Y, He Q, et al. Rectal administration of a chlamydial subunit vaccine protects against genital infection and upper reproductive tract pathology in mice. PLoS One. 2017;12:e0178537. [Internet] Available from
   PubMed Web of Science ®Google Scholar
• Tifrea DF, Pal S, Fairman J, et al. Protection against a chlamydial respiratory challenge by a chimeric vaccine formulated with the Chlamydia muridarum major outer membrane protein variable domains using the Neisseria lactamica porin B as a scaffold. Npj Vaccines. Internet]. 2020;5:37. Available from. https://doi.org/10.1038/s41541-020-0182-9
   PubMed Web of Science ®Google Scholar
• Luan X, Peng B, Li Z, et al. Vaccination with MIP or Pgp3 induces cross-serovar protection against chlamydial genital tract infection in mice. Immunobiology. [Internet]. 2019;224:223–230. Available from https://www.sciencedirect.com/science/article/pii/S0171298518301153
   PubMed Web of Science ®Google Scholar
• Verma R, Sahu R, Dixit S, et al. The Chlamydia M278 major outer membrane peptide encapsulated in the poly(lactic acid)-poly(ethylene glycol) nanoparticulate self-Adjuvanting delivery system protects mice against a chlamydia muridarum genital tract challenge by stimulating robust systemic [Internet]. Front Immunol. 2018;2369. Available from: https://www.frontiersin.org/article/10.3389/fimmu.2018.02369
   PubMed Web of Science ®Google Scholar
• Sahu R, Dixit S, Verma R, et al. A nanovaccine formulation of Chlamydia recombinant MOMP encapsulated in PLGA 85:15 nanoparticles augments CD4+ effector (CD44high CD62Llow) and memory (CD44high CD62Lhigh) T-cells in immunized mice. Nanomedicine Nanotechnology, Biol Med. [Internet] 2020;29:102257. Available from. https://www.sciencedirect.com/science/article/pii/S1549963420301118
   PubMed Web of Science ®Google Scholar
• Rhee JH Chapter 19 - Current and New Approaches for Mucosal Vaccine Delivery .Kiyono H, Pascual DW Mucosal Vaccine 1 (Elsevier Academic Press) editors Mucosal Vaccines [Internet]. 2019 October /25. 2020;325–356. Available from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149853/
   Google Scholar
• Edwards T, Smith J, Sturrock HJW, et al. Prevalence of trachoma in unity state, South Sudan: results from a large-scale population-based survey and potential implications for further surveys. PLoS Negl Trop Dis Internet]. 2012 April /10. 2012;6:e1585–e1585. Available from https://pubmed.ncbi.nlm.nih.gov/22506082
   PubMed Web of Science ®Google Scholar
• Taha MA, Singh SR, Dennis VA. Biodegradable PLGA85/15 nanoparticles as a delivery vehicle for chlamydia trachomatis recombinant MOMP-187 peptide. Nanotechnology. 2012;23:325101.
   PubMed Web of Science ®Google Scholar
• Dixit S, Singh SR, Yilma AN, et al. Poly(lactic acid)-poly(ethylene glycol) nanoparticles provide sustained delivery of a Chlamydia trachomatis recombinant MOMP peptide and potentiate systemic adaptive immune responses in mice. Nanomedicine. 2014;10:1311–1321.
   PubMed Web of Science ®Google Scholar