Sensitivity of nPCR for four types of membrane protein DNA and of two pairs of primers for Tpp47 DNA of Treponema pallidum in whole blood of congenital syphilis newborns

References

• World Health Organization. Global incidence and prevalence of selected curable sexually transmitted infections. Geneva, Switzerland: WHO; 2008. p. 2012.
   Google Scholar
• Newman L, Kamb M, Hawkes S, et al. Global estimates of syphilis in pregnancy and associated adverse outcomes: analysis of multinational antenatal surveillance data. PLoS Med. 2013;10(2):e1001396.
   PubMed Web of Science ®Google Scholar
• Gomez GB, Kamb ML, Newman LM, et al. Untreated maternal syphilis and adverse outcomes of pregnancy: a systematic review and meta-analysis. Bull World Health Organ. 2013;91(3):217–226.
   PubMed Web of Science ®Google Scholar
• Centers for Disease Control. Sexually transmitted diseases treatment guidelines 2010. MMWR Recomm Rep. 2010;59:26–38.
   Google Scholar
• Jim B, Delicia C, Darlene D, et al. STD Surveillance 2014. Division of STD Prevention, November 2015; 53.
   Google Scholar
• Lewis LL. Congenital syphilis. Serologic diagnosis in the young infant. Infect Dis Clin North Am. 1992;6(1):31–39.
   PubMed Web of Science ®Google Scholar
• Rawstron SA, Mehta S, Marcellino L, et al. Congenital syphilis and fluorescent treponemal antibody test reactivity after the age of 1 year. Sex Transm Dis. 2001;28(7):412–416.
   PubMed Web of Science ®Google Scholar
• Hollier LM, Harstad TW, Sanchez PJ, et al. Fetal syphilis: clinical and laboratory characteristics. Obstet Gynecol. 2001;97(6):947–953.
   PubMed Web of Science ®Google Scholar
• Kaufman RE, Olansky DC, Wiesner PJ. The FTA-ABS (IgM) test for neonatal congenital syphilis: a critical review. J Am Vener Dis Assoc. 1974;1(2):79–84.
   PubMedGoogle Scholar
• Centers for Disease Control and Prevention. Sexually transmitted diseases treatment guidelines, 2006. MMWR Recomm Rep. 2006;55(RR-1):22–36.
   Google Scholar
• Larsen SA, Steiner BM, Rudolph AH. Laboratory diagnosis and interpretation of tests for syphilis. Clin Microbiol Rev. 1995;8(1):1–21.
   PubMed Web of Science ®Google Scholar
• Lafond RE, Lukehart SA. Biological basis for syphilis. Clin Microbiol Rev. 2006;19(1):29–49.
   PubMed Web of Science ®Google Scholar
• Sánchez PJ, Wendel GD Jr, Grimprel E, et al. Evaluation of molecular methodologies and rabbit infectivity testing for the diagnosis of congenital syphilis and neonatal central nervous system invasion by Treponema pallidum. J Infect Dis. 1993;167(1):148–157.
   PubMed Web of Science ®Google Scholar
• Grimprel E, Sánchez PJ, Wendel GD, et al. Use of polymerase chain reaction and rabbit infectivity testing to detect Treponema pallidum in amniotic fluid, fetal and neonatal sera, and cerebrospinal fluid. J Clin Microbiol. 1991;29(8):1711–1718.
   PubMed Web of Science ®Google Scholar
• Hong FC, Yang YZ, Liu XL, et al. Reduction in mother-to-child transmission of syphilis for 10 years in Shenzhen, China. Sex Transm Dis. 2014;41(3):188–193.
   PubMed Web of Science ®Google Scholar
• Catalin A, Brusin S, Joanna GD, et al. European Centre for Disease Prevention and Control. Annual epidemiological report 2014 – sexually transmitted infections, including HIV and blood-borne viruses, vol. 2. Stockholm: ECDC; 2015. p. 32–34.
   Google Scholar
• Genç M, Ledger WJ. Syphilis in pregnancy. Sex Transm Infect. 2000;76(2):73–79.
   PubMed Web of Science ®Google Scholar
• Woods CR. Congenital syphilis – persisting pestilence. Pediatr Infect Dis J. 2009;28(6):536–537
   PubMed Web of Science ®Google Scholar
• Brion LP, Manuli M, Rai B, et al. Long-bone radiographic abnormalities as a sign of active congenital syphilis in asymptomatic newborns. Pediatrics. 1991;88(5):1037–1040.
   PubMed Web of Science ®Google Scholar
• Velasquez C, Guerra-Giraldez C, Giacani L, et al. Congenital syphilis. In: Gupta S, Kumar B, editors. Sexually Transmitted Infections. New Delhi: Elsevier; 2011. p. 828–834.
   Google Scholar
• Grange PA, Gressier L, Dion PL, et al. Evaluation of a PCR test for detection of Treponema pallidum in swabs and blood. J Clin Microbiol. 2012;50(3):546–552.
   PubMed Web of Science ®Google Scholar
• Kouznetsov AV, Weisenseel P, Trommler P, et al. Detection of the 47-kilodalton membrane immunogen gene of Treponema pallidum in various tissue sources of patients with syphilis. Diagn Microbiol Infect Dis. 2005;51(2):143–145.
   PubMed Web of Science ®Google Scholar
• Pietravalle M, Pimpinelli F, Maini A, et al. Diagnostic relevance of polymerase chain reaction technology for T. pallidum in subjects with syphilis in different phases of infection. New Microbiol. 1999;22(2):99–104.
   PubMed Web of Science ®Google Scholar
• Salazar JC, Rathi A, Michael NL, et al. Assessment of the kinetics of Treponema pallidum dissemination into blood and tissues in experimental syphilis by real-time quantitative PCR. Infect Immun. 2007;75(6):2954–2958.
   PubMed Web of Science ®Google Scholar
• United States Public Health Service. Syphilis, a synopsis. Publication no. 1660. Washington, DC: U.S. Government Printing Office; 1968.
   Google Scholar
• Rodionova EN, Gushchin AE, Shipulin GA, et al. Detection of Treponema pallidum DNA and RNA in clinical material from patients with syphilis at different stages. Zh Mikrobiol Epidemiol Immunobiol. 2003;3(3):43–50.
   Google Scholar
• Cruz AR, Pillay A, Zuluaga AV, et al. Secondary syphilis in cali, Colombia: new concepts in disease pathogenesis. PLoS Negl Trop Dis. 2010;4(5):e690.
   PubMed Web of Science ®Google Scholar
• Tipple C, Hanna MO, Hill S, et al. Getting the measure of syphilis: qPCR to better understand early infection. Sex Transm Infect. 2011;87(6):479–485
   PubMed Web of Science ®Google Scholar
• Gayet-Ageron A, Lautenschlager S, Ninet B, et al. Sensitivity, specificity and likelihood ratios of PCR in the diagnosis of syphilis: a systematic review and meta-analysis. Sex Transm Infect. 2013;89(3):251–256.
   PubMed Web of Science ®Google Scholar
• Zoechling N, Schluepen EM, Soyer HP, et al. Molecular detection of Treponema pallidum in secondary and tertiary syphilis. Br J Dermatol. 1997;136(5):683–686.
   PubMed Web of Science ®Google Scholar
• Martin IE, Tsang RS, Sutherland K, et al. Molecular characterization of syphilis in patients in Canada: azithromycin resistance and detection of Treponema pallidum DNA in whole-blood samples versus ulcerative swabs. J Clin Microbiol. 2009;47(6):1668–1673.
   PubMed Web of Science ®Google Scholar
• Wicher K, Horowitz HW, Wicher V. Laboratory methods of diagnosis of syphilis for the beginning of the third millennium. Microbes Infect. 1999;1(12):1035–1049.
   PubMed Web of Science ®Google Scholar
• Marfin AA, Liu H, Sutton MY, et al. Amplification of the DNA polymerase I gene of Treponema pallidum from whole blood of persons with syphilis. Diagn Microbiol Infect Dis. 2001;40(4):163–166.
   PubMed Web of Science ®Google Scholar
• Magnuson HJ, Thomas EW, Olansky S, et al. Inoculation syphilis in human volunteers. Medicine (Baltimore). 1956;35(1):33–82.
   PubMed Web of Science ®Google Scholar
• Cumberland MC, Turner TB. The rate of multiplication of Treponema pallidum in normal and immune rabbits. Am J Syph Gonorrhea Vener Dis. 1949;33(3):201–212.
   PubMedGoogle Scholar
• Magnuson HJ, Eagle H, Fleischman R. The minimal infectious inoculum of Spirochaeta pallida (Nichols strain), and a consideration of its rate of multiplication in vivo. Am J Syph Gonorrhea Vener Dis. 1948;32(1):1–18.
   PubMedGoogle Scholar
• Kwok S, Higuchi R. Avoiding false positives with PCR. Nature. 1989;339(6221):237–238.
   PubMed Web of Science ®Google Scholar