Hidden in the litter: cryptic diversity of the leaf-litter toad Rhinella castaneotica–proboscidea complex revealed through integrative taxonomy, with description of a new species from south-western Amazonia

References

• Angulo, A., & Icochea, J. (2010). Cryptic species complexes, widespread species and conservation: lessons from Amazonian frogs of the Leptodactylus marmoratus group (Anura: Leptodactylidae). Systematics and Biodiversity, 8(3), 357–370. https://doi.org/10.1080/14772000.2010.507264
   Web of Science ®Google Scholar
• Ávila, R. W., Morais, D. H., Perez, R., Pansonato, A., Carvalho, V. T., Rojas, R. R., Gordo, M., & Farias, I. P. (2020). A new species of the Rhinella margaritifera (Laurenti 1768) species group (Anura, Bufonidae) from southern Brazilian Amazonia. Zootaxa, 4868(3), zootaxa.4868.3.3–388. https://doi.org/10.11646/zootaxa.4868.3.3
   Web of Science ®Google Scholar
• Ávila, R. W., Pansonato, A., Perez, R., Carvalho, V. T., Roberto, I. J., Morais, D. H., De Almeida, A. P., Rojas, R. R., Gordo, M., & Farias, I. P. (2018). On Rhinella gildae Vaz-Silva, Maciel, Bastos & Pombal 2015 (Anura: Bufonidae): Phylogenetic relationship, morphological variation, advertisement and release calls and geographic distribution. Zootaxa, 4462(2), 274–290. https://doi.org/10.11646/zootaxa.4462.2.9
   PubMed Web of Science ®Google Scholar
• Ávila, R. W., Pansonato, A., & Strüssmann, C. (2010). A new species of the Rhinella margaritifera group (Anura: Bufonidae) from Brazilian Pantanal. Zootaxa, 2339(1), 57–68. https://doi.org/10.11646/zootaxa.2339.1.3
   Google Scholar
• Barni, P. E., Fearnside, P. M., & Graça, P. M. L. A. (2015). Simulating deforestation and carbon loss in Amazonia: Impacts in Brazil's Roraima state from reconstructing Highway BR-319 (Manaus-Porto Velho). Environmental Management, 55(2), 259–278. https://doi.org/10.1007/s00267-014-0408-6
   PubMed Web of Science ®Google Scholar
• Bioacoustics Research Program. (2014). Raven Pro: Interactive sound analysis software. Version 1.5. [Computer software]. The Cornell Lab of Ornithology, USA.
   Google Scholar
• Bouckaert, R., Heled, J., Kühnert, D., Vaughan, T., Wu, C.-H., Xie, D., Suchard, M. A., Rambaut, A., & Drummond, A. J. (2014). BEAST 2: a software platform for Bayesian evolutionary analysis. PLoS Computational Biology, 10(4), e1003537. https://doi.org/10.1371/journal.pcbi.1003537
   PubMed Web of Science ®Google Scholar
• Brando, P. M., Soares-Filho, B., Rodrigues, L., Assunção, A., Morton, D., Tuchschneider, D., Fernandes, E. C. M., Macedo, M. N., Oliveira, U., & Coe, M. T. (2020). The gathering firestorm in southern Amazonia. Science Advances, 6(2), eeay1632. https://doi.org/10.1126/sciadv.aay1632
   Web of Science ®Google Scholar
• Breiman, L. (2001). Random forests. Machine Learning, 45(1), 5–32. https://doi.org/10.1023/A:1010933404324
   Web of Science ®Google Scholar
• Caldwell, J. P. (1991). A new species of toad in the genus Bufo from Pará, Brazil, with an unusual breeding site. Papéis Avulsos de Zoologia, 37, 389–400.
   Google Scholar
• Caldwell, J. P., & Shepard, D. B. (2007). Calling site fidelity and call structure of a Neotropical toad, Rhinella ocellata (Anura: Bufonidae). Journal of Herpetology, 41(4), 611–621. https://doi.org/10.1670/07-025.1
   Web of Science ®Google Scholar
• Caminer, M. A., Milá, B., Jansen, M., Fouquet, A., Venegas, P. J., Chávez, G., Lougheed, S. C., & Ron, S. R. (2017). Systematics of the Dendropsophus leucophyllatus species complex (Anura: Hylidae): Cryptic diversity and the description of two new species. PLoS One, 12(3), e0171785. https://doi.org/10.1371/journal.pone.0171785
   PubMed Web of Science ®Google Scholar
• Caramaschi, U., & Niemeyer, H. (2003). Nova espécie do complexo de Bufo margaritifer (Laurenti, 1768) do Estado do Mato Grosso do Sul, Brasil (Amphibia, Anura, Bufonidae). Boletim do Museu Nacional, new series, Zoologia, 501, 1–16.
   Google Scholar
• Caramaschi, U., & Pombal, J. P. (2006). A new species of Rhinella Fitzinger, 1826 from the Atlantic rain forest, eastern Brazil (Amphibia, Anura, Bufonidae). Papéis Avulsos de Zoologia, 46(23), 251–259. https://doi.org/10.1590/S0031-10492006002300001
   Google Scholar
• Carvalho, T. R., Angulo, A., Kokubum, M. N. C., Barrera, D. A., Souza, M. B., Haddad, C. F. B.,…Giaretta, A. A. (2019). A new cryptic species of the Adenomera andreae clade from southwestern Amazonia (Anura, Leptodactylidae). Herpetologica, 75, 233–246. https://doi.org/10.1655/D-18-00049
   Web of Science ®Google Scholar
• Carvalho, T. R., Moraes, L. J. C. L., Lima, A. P., Fouquet, A., Peloso, P. L. V., Pavan, D., … Haddad, C. F. B. (2021). Systematics and historical biogeography of Neotropical foam-nesting frogs of the Adenomera heyeri clade (Leptodactylidae), with the description of six new Amazonian species. Zoological Journal of the Linnean Society, 191, 395–433.
   Web of Science ®Google Scholar
• Darriba, D., Taboada, G. L., Doallo, R., & Posada, D. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9(8), 772.
   PubMed Web of Science ®Google Scholar
• De la Riva, I., Köhler, J., Lötters, S., & Reichle, S. (2000). Ten years of research on Bolivian amphibians: updated checklist, distribution, taxonomic problems, literature, and iconography. Revista Española de Herpetología, 14, 19–164.
   Google Scholar
• Dittmann, D. (2016). LSUMZ Herp Tissues. Version 3.1. Louisiana State University Museum of Natural Science. Occurrence dataset. https://doi.org/10.15468/kuzcfv
   Google Scholar
• Duellman, W. E. (1970). The hylid frogs of Middle America 1. Monograph of the Museum of Natural History, the University of Kansas, 1, 1–428.
   Google Scholar
• Engel, M. S., Ceríaco, L. M. P., Daniel, G. M., Dellapé, P. M., Löbl, I., Marinov, M., Reis, R. E., Young, M. T., Dubois, A., Agarwal, I., Lehmann A, P., Alvarado, M., Alvarez, N., Andreone, F., Araujo-Vieira, K., Ascher, J. S., Baêta, D., Baldo, D., Bandeira, S. A., … Zacharie, C. K. (2021). The taxonomic impediment: a shortage of taxonomists, not the lack of technical approaches. Zoological Journal of the Linnean Society, 193(2), 381–387. https://doi.org/10.1093/zoolinnean/zlab072
   Web of Science ®Google Scholar
• Ferrão, M., Colatreli, O., Fraga, R., Kaefer, I. L., Moravec, J., & Lima, A. P. (2016). High species richness of Scinax treefrogs (Hylidae) in a threatened Amazonian landscape revealed by an integrative approach. PLoS One, 11(11), e0165679. https://doi.org/10.1371/journal.pone.0165679
   PubMed Web of Science ®Google Scholar
• Ferrão, M., Lima, A. P., Ron, S., Santos, S. P., & Hanken, J. (2020a). New species of leaf-litter toad of the Rhinella margaritifera species group (Anura: Bufonidae) from Amazonia. Copeia, 108(4), 967–986. https://doi.org/10.1643/CH2020043
   Web of Science ®Google Scholar
• Ferrão, M., Moravec, J., Fraga, R., Almeida, A. P., Kaefer, I. L., & Lima, A. P. (2017). A new species of Scinax from the Purus-Madeira interfluve, Brazilian Amazonia (Anura, Hylidae). ZooKeys, 706, 137–162. https://doi.org/10.3897/zookeys.706.14691
   Google Scholar
• Ferrão, M., Moravec, J., Hanken, J., & Lima, A. P. (2020b). A new species of Dendropsophus (Anura, Hylidae) from southwestern Amazonia with a green bilobate vocal sac. ZooKeys, 942, 77–104. https://doi.org/10.3897/zookeys.942.51864
   PubMedGoogle Scholar
• Fouquet, A., Gaucher, P., Blanc, M., & Vélez-Rodriguez, C. M. (2007b). Description of two new species of Rhinella (Anura: Bufonidae) from the lowlands of the Guiana Shield. Zootaxa, 1663, 17–32.
   Google Scholar
• Fouquet, A., Ledoux, J. B., Dubut, V., Noonan, B. P., & Scotti, I. (2012c). The interplay of dispersal limitation, rivers, and historical events shapes the genetic structure of an Amazonian frog. Biological Journal of the Linnean Society, 106(2), 356–373. https://doi.org/10.1111/j.1095-8312.2012.01871.x
   Web of Science ®Google Scholar
• Fouquet, A., Marinho, P., Réjaud, A., Carvalho, T. R., Caminer, M. A., Jansen, M., Rainha, R. N., Rodrigues, M. T., Werneck, F. P., Lima, A. P., Hrbek, T., Giaretta, A. A., Venegas, P. J., Chávez, G., & Ron, S. (2021). Systematics and biogeography of the Boana albopunctata species group (Anura, Hylidae), with the description of two new species from Amazonia. Systematics and Biodiversity, 19(4), 375–399. https://doi.org/10.1080/14772000.2021.1873869
   Web of Science ®Google Scholar
• Fouquet, A., Martinez, Q., Zeidler, L., Courtois, E. A., Gaucher, P., Blanc, M., Lima, J. D., Souza, S. M., Rodrigues, M. T., Souza, S. M., Rodrigues, M. T., & Kok, P. J. R. (2016). Cryptic diversity in the Hypsiboas semilineatus species group (Amphibia, Anura) with the description of a new species from the eastern Guiana Shield. Zootaxa, 4084(1), 79–104. https://doi.org/10.11646/zootaxa.4084.1.3
   PubMed Web of Science ®Google Scholar
• Fouquet, A., Noonan, B. P., Rodrigues, M. T., Pech, N., Gilles, A., & Gemmell, N. J. (2012b). Multiple Quaternary refugia in the eastern Guiana Shield revealed by comparative phylogeography of 12 frog species. Systematic Biology, 61(3), 461–489. https://doi.org/10.1093/sysbio/syr130
   PubMed Web of Science ®Google Scholar
• Fouquet, A., Recoder, R., Teixeira, M., Cassimiro, J., Amaro, R. C., Camacho, A., Damasceno, R., Carnaval, A. C., Moritz, C., & Rodrigues, M. T. (2012a). Molecular phylogeny and morphometric analyses reveal deep divergence between Amazonia and Atlantic forest species of Dendrophryniscus. Molecular Phylogenetics and Evolution, 62(3), 826–838. https://doi.org/10.1016/j.ympev.2011.11.023
   PubMed Web of Science ®Google Scholar
• Fouquet, A., Vences, M., Salducci, M. D., Meyer, A., Marty, C., Blanc, M., & Gilles, A. (2007a). Revealing cryptic diversity using molecular phylogenetics and phylogeography in frogs of the Scinax ruber and Rhinella margaritifera species groups. Molecular Phylogenetics and Evolution, 43(2), 567–582. https://doi.org/10.1016/j.ympev.2006.12.006
   PubMed Web of Science ®Google Scholar
• Fox, J., & Weisberg, S. (2019). An R Companion to Applied Regression. Thousand Oaks CA.
   Google Scholar
• Frost, D. R. (2021). Amphibian Species of the World: an Online Reference. Version 6.0 (Accessed 3 September 2021). Electronic Database accessible at http://research.amnh.org/herpetology/amphibia/index.html. American Museum of Natural History, New York, USA.
   Google Scholar
• Gehara, M., Crawford, A. J., Orrico, V. G. D., Rodríguez, A., Lötters, S., Fouquet, A., Barrientos, L. S., Brusquetti, F., De la Riva, I., Ernst, R., Urrutia, G. G., Glaw, F., Guayasamin, J. M., Hölting, M., Jansen, M., Kok, P. J. R., Kwet, A., Lingnau, R., Lyra, M., … Köhler, J. (2014). High levels of diversity uncovered in a widespread nominal taxon: Continental phylogeography of the Neotropical tree frog Dendropsophus minutus. PLoS One, 9(9), e103958. https://doi.org/10.1371/journal.pone.0103958
   PubMed Web of Science ®Google Scholar
• Gingras, B., & Fitch, W. T. (2013). A three-parameter model for classifying anurans into four genera based on advertisement calls. The Journal of the Acoustical Society of America, 133(1), 547–559.
   PubMed Web of Science ®Google Scholar
• Günther, A. C. L. G. (1858). Neue Batrachier in der Sammlung des Britischen Museums. Archiv Für Naturgeschichte. 24, 319–328. https://doi.org/10.5962/bhl.part.5288
   Google Scholar
• Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
   Google Scholar
• Hoang, D. T., Chernomor, O., von Haeseler, A., Minh, B. Q., & Vinh, L. S. (2018). UFBoot2: Improving the Ultrafast Bootstrap Approximation. Molecular Biology and Evolution, 35(2), 518–522.
   PubMed Web of Science ®Google Scholar
• Hoogmoed, M. S. (1986). Biosystematic studies of the Bufo “typhonius” group. A preliminary progress report. In Z. Rocek (Ed.), Studies in Herpetology. (pp. 147–150). Charles University.
   Google Scholar
• Hoogmoed, M. S. (1990). Biosystematics of South American Bufonidae with special reference to the Bufo “typhonius” group. In G. Peters & R. Hutterer (Eds.), Vertebrates in the Tropics. (pp. 113–123). Museum Alexander Koenig.
   Google Scholar
• Izzo, T. J., Rodrigues, D. J., Menin, M., Lima, A. P., & Magnusson, W. E. (2012). Functional necrophilia: a profitable anuran reproductive strategy? Journal of Natural History, 46(47-48), 2961–2967. https://doi.org/10.1080/00222933.2012.724720
   Web of Science ®Google Scholar
• Jansen, M., Bloch, R., Schulze, A., & Pfenninger, M. (2011). Integrative inventory of Bolivia’s lowland anurans reveals hidden diversity. Zoologica Scripta, 40(6), 567–583. https://doi.org/10.1111/j.1463-6409.2011.00498.x
   Web of Science ®Google Scholar
• Jiménez de la Espada, M. (1875). Vertebrados del viaje al Pacífico verificado de 1862 a 1865 por una comisión de naturalistas enviada por el Gobierno Español. Batracios. A. Miguel Ginesta.
   Google Scholar
• Jombart, T., & Ahmed, I. (2011). Adegenet 1.3-1: New tools for the analysis of genome-wide SNP data. Bioinformatics (Oxford, England), 27(21), 3070–3071.
   PubMed Web of Science ®Google Scholar
• Jombart, T., Devillard, S., & Balloux, F. (2010). Discriminant analysis of principal components: a new method for the analysis of genetically structured populations. BMC Genetics, 11, 94. https://doi.org/10.1186/1471-2156-11-94
   PubMed Web of Science ®Google Scholar
• Jorge, R. F., Ferrão, M., & Lima, A. P. (2020). Out of bound: A new threatened harlequin toad (Bufonidae, Atelopus) from the outer borders of the Guiana Shield in Central Amazonia described through integrative taxonomy. Diversity, 12(8), 310. https://doi.org/10.3390/d12080310
   Google Scholar
• Jungfer, K.-H., Faivovich, J., Padial, J. M., Castroviejo-Fisher, S., Lyra, M. M., V. M. Berneck, B., Iglesias, P. P., Kok, P. J. R., MacCulloch, R. D., Rodrigues, M. T., Verdade, V. K., Torres Gastello, C. P., Chaparro, J. C., Valdujo, P. H., Reichle, S., Moravec, J., Gvoždík, V., Gagliardi-Urrutia, G., Ernst, R., … F. B. Haddad, C. (2013). Systematics of spiny-backed treefrogs (Hylidae: Osteocephalus): an Amazonian puzzle. Zoologica Scripta, 42(4), 351–380. https://doi.org/10.1111/zsc.12015
   Web of Science ®Google Scholar
• Kaefer, I. L., Tsuji-Nishikido, B. M., & Lima, A. P. (2012). Beyond the river: Underlying determinants of population acoustic signal variability in Amazonian direct-developing Allobates (Anura: Dendrobatoidea). Acta Ethologica, 15(2), 187–194. https://doi.org/10.1007/s10211-012-0126-0
   Web of Science ®Google Scholar
• Kalamandeen, M., Gloor, E., Mitchard, E., Quincey, D., Ziv, G., Spracklen, D., … Galbraith, D. (2018). Pervasive rise of small-scale deforestation in Amazonia. Science Report, 8, 1600.
   PubMed Web of Science ®Google Scholar
• Köhler, J., Jansen, M., Rodríguez, A., Kok, P. J. R., Toledo, L. F., Emmrich, M., Glaw, F., Haddad, C. F. B., Rödel, M.-O., & Vences, M. (2017). The use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice. Zootaxa, 4251(1), 1–124. https://doi.org/10.11646/zootaxa.4251.1.1
   PubMed Web of Science ®Google Scholar
• Köhler, J., & Lötters, S. (1999). Annotated list of amphibian records from the Departamento Pando, Bolivia, with description of some advertisement calls. Bonner Zoologische Beiträge, 48, 259–273.
   Google Scholar
• Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547–1549.
   PubMed Web of Science ®Google Scholar
• Laurenti, J. N. (1768). Specimen Medicum, Exhibens Synopsin Reptilium Emendatum cum Experimentis Circa Venena et Antidota Reptilium Austriacorum. Joan. Thom. nob. de Trattnern.
   Google Scholar
• Liaw, A., & Wiener, M. (2002). Classification and regression by RandomForest. R News, 2, 18–22.
   Google Scholar
• Ligges, U., Krey, S., Mersmann, O., Schnackenberg, S. (2017). tuneR: analysis of music and speech. https://CRAN.R-project.org/package=tuneR.
   Google Scholar
• Lima, L. R., Bruschi, D. P., Do Nascimento, F. A. C., Scherrer De Araújo, P. V., Costa, L. P., Thomé, M. T. C., Garda, A. A., Zattera, M. L., & Mott, T. (2020). Below the waterline: cryptic diversity of aquatic pipid frogs (Pipa carvalhoi) unveiled through an integrative taxonomy approach. Systematics and Biodiversity, 18(8), 771–783. https://doi.org/10.1080/14772000.2020.1795742
   Web of Science ®Google Scholar
• Lima, A. P., Ferrão, M., & Silva, D. L. (2020). Not as widespread as thought: Integrative taxonomy reveals cryptic diversity in the Amazonian nurse frog Allobates tinae Melo‐Sampaio, Oliveira and Prates, 2018 and description of a new species. Journal of Zoological Systematics and Evolutionary Research, 58(4), 1173–1194. https://doi.org/10.1111/jzs.12406
   Web of Science ®Google Scholar
• Lima, A. P., Magnusson, W. E., Menin, M., Erdtmann, L. K., Rodrigues, D. J., Keller, C., & Hödl, W. (2012). Guide to the Frogs of Reserva Ducke - Central Amazonia (2nd ed.). Editora INPA.
   Google Scholar
• Lima, A. P., Menin, M., & Araújo, M. C. (2007). A new species of Rhinella (Anura: Bufonidae) from Brazilian Amazon. Zootaxa, 1663, 1–15.
   Google Scholar
• Lötters, S., & Köhler, J. (2000). A new toad of the Bufo typhonius complex from humid montane forests of Bolivia. Spixiana, 23, 293–303.
   Google Scholar
• Magnusson, W. E., Braga-Neto, R., Pezzini, F., Baccaro, F., Bergallo, H., Penha, J., … Pontes, A. R. M. (2013). Biodiversity and Integrated Environmental Monitoring. Attema.
   Google Scholar
• Melin, D. (1941). Contributions to the knowledge of the Amphibia of South America. Göteborgs Kungl. Vetenskaps-Och Vitterhets-Samhälles Handllingar, 6, 1–71.
   Google Scholar
• Melo‐Sampaio, P. R., Oliveira, R. M., & Prates, I. (2018). A new nurse frog from Brazil (Aromobatidae: Allobates). with data on the distribution and phenotypic variation of western Amazonian species. South American Journal of Herpetology, 13(2), 131–149. https://doi.org/10.2994/SAJH-D-17-00098.1
   Web of Science ®Google Scholar
• Melo-Sampaio, P. R., Ferrão, M., & Moraes, L. J. C. L. (2021). A new species of Osteocephalus Steindachner, 1862 (Anura, Hylidae), from Brazilian Amazonia. Breviora, 572, 1–21. https://doi.org/10.3099/0006-9698-572.1.1
   Google Scholar
• Mijares-Urrutia, A., & Arends, A. (2001). A new toad of the Bufo margaritifer complex (Amphibia: Bufonidae) from northwestern Venezuela. Herpetologica, 57, 523–531.
   Web of Science ®Google Scholar
• Moravec, J., Aparicio, J., Guerrero-Reinhard, M., & Calderon, G. (2011). Anuran species richness in the Departamento Pando, Bolivia. Bonner Zoologische Monographienp, 57, 47–57.
   Google Scholar
• Moravec, J., Lehr, E., Cusi, J. C., Córdova, J. H., & Gvoždík, V. (2014). A new species of the Rhinella margaritifera species group (Anura, Bufonidae) from the montane forest of the Selva Central, Peru. ZooKeys, 371, 35–56. https://doi.org/10.3897/zookeys.371.6580
   Google Scholar
• Mota, E. P., Kaefer, I. L., Nunes, M. d S., Lima, A. P., & Farias, I. P. (2020). Hidden diversity within the broadly distributed Amazonian giant monkey frog (Phyllomedusa bicolor: Phyllomedusidae). Amphibia-Reptilia, 41(3), 349–359. https://doi.org/10.1163/15685381-bja10003
   Web of Science ®Google Scholar
• Motta, J., Menin, M., Almeida, A. P., Hrbek, T., & Farias, I. P. (2018). When the unknown lives next door: a study of central Amazonian anurofauna. Zootaxa, 4438(1), 79–104.
   PubMed Web of Science ®Google Scholar
• Myers, G. S., & Carvalho, A. L. (1945). Notes on some new or little-known Brazilian amphibians, with an examination of the history of the plata salamander, Ensatina platensis. Boletim Do Museu Nacional, New Series, Zoologia, 35, 1–24.
   Google Scholar
• Myers, C. W., & Duellman, W. E. (1982). A new species of Hyla from Cerro Colorado, and other treefrog records and geographical notes from western Panama. American Museum Novitates, 2752, 1–32.
   Google Scholar
• Nguyen, L. T., Schmidt, H. A., von Haeseler, A., & Minh, B. Q. (2015). IQ-TREE: A fast and effective stochastic algorithm for estimating Maximum-Likelihood phylogenies. Molecular Biology and Evolution, 32(1), 268–274.
   PubMed Web of Science ®Google Scholar
• Palumbi, S. R. (1991). Nucleic acids II: the polymerase chain reaction. In D. M. Hillis, C. Moritz, & B. K. Mable (Eds.), Molecular Systematics (pp. 205–247). Sinauer & Associates Inc.
   Google Scholar
• Peloso, P. L. V., Faivovich, J., Grant, T., Gasparini, J. L., & Haddad, C. F. B. (2012). An extraordinary new species of Melanophryniscus (Anura, Bufonidae) from southeastern Brazil. American Museum Novitates, 3762(3762), 1–32. https://doi.org/10.1206/3762.2
   Google Scholar
• Pentinsaari, M., Vos, R., & Mutanen, M. (2017). Algorithmic single-locus species delimitation: effects of sampling effort, variation and nonmonophyly in four methods and 1870 species of beetles. Molecular Ecology Resources, 17(3), 393–404. https://doi.org/10.1111/1755-0998.12557
   PubMed Web of Science ®Google Scholar
• Pereyra, M. O., Blotto, B. L., Baldo, D., Chaparro, J. C., Ron, S. R., Elias-Costa, A. J., Iglesias, P. P., Venegas, P. J., C. Thomé, M. T., Ospina-Sarria, J. J., Maciel, N. M., Rada, M., Kolenc, F., Borteiro, C., Rivera-Correa, M., Rojas-Runjaic, F. J. M., Moravec, J., De La Riva, I., Wheeler, W. C., … Faivovich, J. (2021). Evolution in the genus Rhinella: a total evidence phylogenetic analysis of neotropical true toads (Anura: Bufonidae). Bulletin of the American Museum of Natural History, 447(1), 156. https://doi.org/10.1206/0003-0090.447.1.1
   Google Scholar
• Piontekowski, V. J., Ribeiro, F. P., Matricardi, E. A. T., Lustosa Junior, I. M., Bussinguer, A. P., & Gatto, A. (2019). Modeling deforestation in the state of Rondônia. Floresta e Ambiente, 26(3), e20180441. https://doi.org/10.1590/2179-8087.044118
   Web of Science ®Google Scholar
• Pramuk, J. B. (2006). Phylogeny of South American Bufo (Anura: Bufonidae) inferred from combined evidence. Zoological Journal of the Linnean Society, 146(3), 407–452. https://doi.org/10.1111/j.1096-3642.2006.00212.x
   Web of Science ®Google Scholar
• Puillandre, N., Lambert, A., Brouillet, S., & Achaz, G. (2012). ABGD, automatic barcode gap discovery for primary species delimitation. Molecular Ecology, 21(8), 1864–1877. https://doi.org/10.1111/j.1365-294X.2011.05239.x
   PubMed Web of Science ®Google Scholar
• R Core Team. (2018). R: A language and environment for statistical computing. R Foundation for Statistical Computing.
   Google Scholar
• Rambaut, A., Drummond, A. J., Xie, D., Baele, G., & Suchard, M. A. (2018). Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Systematic Biology, 67(5), 901–904. https://doi.org/10.1093/sysbio/syy032
   PubMed Web of Science ®Google Scholar
• Rivadeneira, C. D., Venegas, P. J., & Ron, S. R. (2018). Species limits within the widespread Amazonian treefrog Dendropsophus parviceps with descriptions of two new species (Anura, Hylidae). ZooKeys, 726, 25–77. https://doi.org/10.3897/zookeys.726.13864
   Google Scholar
• Roberto, I. J., Brito, L., & Cascon, P. (2011). Temporal and spatial patterns of reproductive activity in Rhinella hoogmoedi (Anura: Bufonidae) from a tropical rainforest in northeastern Brazil, with the description of its advertisement call. South American Journal of Herpetology, 6(2), 87–97. https://doi.org/10.2994/057.006.0207
   Google Scholar
• Rojas, R. R., Carvalho, V. T., Gordo, M., Avila, R. W., Farias, I. P., & Hrbek, T. (2014). A new species of Amazophrynella (Anura: Bufonidae) from the southwestern part of the Brazilian Guiana Shield. Zootaxa, 3753, 79–95.
   PubMed Web of Science ®Google Scholar
• Rojas, R. R., Fouquet, A., Ron, S. R., Hernández-Ruz, E. J., Melo-Sampaio, P. R., Chaparro, J. C., Vogt, R. C., Carvalho, V. T., Pinheiro, L. C., Avila, R. W., Farias, I. P., Gordo, M., & Hrbek, T. (2018). A Pan-Amazonian species delimitation: high species diversity within the genus Amazophrynella (Anura: Bufonidae). PeerJ., 6, e4941.
   PubMed Web of Science ®Google Scholar
• Santos, S. P., IBánez, R., & Ron, S. R. (2015). Systematics of the Rhinella margaritifera complex (Anura, Bufonidae) from western Ecuador and Panama with insights in the biogeography of Rhinella alata. ZooKeys, 501, 109–145. https://doi.org/10.3897/zookeys.501.8604
   Google Scholar
• Savage, J. M., & Heyer, W. R. (1967). Variation and distribution of the treefrog genus Phyllomedusa in Costa Rica, Central America. Beitrage Zur Neotropischen Fauna, 5(2), 111–131. https://doi.org/10.1080/01650526709360400
   Google Scholar
• Simões, P. I., Lima, A. P., & Farias, I. P. (2010). The description of a cryptic species related to the pan-Amazonian frog Allobates femoralis (Boulenger 1883) (Anura: Aromobatidae). Zootaxa, 2406(1), 1–28. https://doi.org/10.11646/zootaxa.2406.1.1
   Google Scholar
• Simões, P. I., Stow, A., Hödl, W., Amézquita, A., Farias, I. P., & Lima, A. P. (2014). The value of including intraspecific measures of biodiversity in environmental impact surveys is highlighted by the Amazonian brilliant-thighed frog (Allobates femoralis). Tropical Conservation Science, 7(4), 811–828. https://doi.org/10.1177/194008291400700416
   Web of Science ®Google Scholar
• Siqueira-Gay, J., & Sánchez, L. E. (2021). The outbreak of illegal gold mining in the Brazilian Amazon boosts deforestation. Regional Environmental Change, 21(2), 28. https://doi.org/10.1007/s10113-021-01761-7
   Web of Science ®Google Scholar
• Spix, J. B. v. (1824). Animalia nova sive Species novae Testudinum et Ranarum quas in itinere per Brasiliam annis MDCCCXVII–MDCCCXX jussu et auspiciis Maximiliani Josephi I. Bavariae Regis. F. S. Hübschmann.
   Google Scholar
• Stynoski, J. L., Trama, F. A., Rizo-Patrón, F. L., Tapia, E., & Hoke, K. L. (2020). Reproductive ecology of the Peruvian earless toad Rhinella yunga (Amphibia, Bufonidae) with descriptions of calls, tadpole, and female competition. South American Journal of Herpetology, 2020(15), 85–96. https://doi.org/10.2994/SAJH-D-18-00030.1
   Google Scholar
• Sueur, J., Aubin, T., & Simonis, C. (2008). Seewave, a free modular tool for sound analysis and synthesis. Bioacoustics, 18(2), 213–226. https://doi.org/10.1080/09524622.2008.9753600
   Web of Science ®Google Scholar
• Thominot, A. (1884). Note sur un batracien d’espèce nouvelle provenant de Panama. Bulletin de la Société Philomathique de Paris. Series, 7(8), 151–152.
   Google Scholar
• Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and wight matrix choice. Nucleic Acids Research, 22(22), 4673–4680. https://doi.org/10.1093/nar/22.22.4673
   PubMed Web of Science ®Google Scholar
• Trifinopoulos, J., Nguyen, L. T., von Haeseler, A., & Minh, B. Q. (2016). W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research, 44(W1), W232–235. https://doi.org/10.1093/nar/gkw256
   PubMed Web of Science ®Google Scholar
• Vacher, J. ‐P., Chave, J., Ficetola, F. G., Sommeria‐Klein, G., Tao, S., Thébaud, C., Blanc, M., Camacho, A., Cassimiro, J., Colston, T. J., Dewynter, M., Ernst, R., Gaucher, P., Gomes, J. O., Jairam, R., Kok, P. J. R., Lima, J. D., Martinez, Q., Marty, C., … Fouquet, A. (2020). Large‐scale DNA‐based survey of frogs in Amazonia suggests a vast underestimation of species richness and endemism. Journal of Biogeography, 47(8), 1781–1791. https://doi.org/10.1111/jbi.13847
   Web of Science ®Google Scholar
• Valencia-Zuleta, A., Guerra, V., Carvalho, M. A. G., Costa, N. Q., Faria, D. C. C., Bastos, R. P., & Maciel, N. M. (2020). Vocalizations of Rhinella sebbeni Vaz-Silva, Maciel, Bastos, and Pombal, 2015 (Anura: Bufonidae). Bioacoustics, 29(2), 197–209. https://doi.org/10.1080/09524622.2018.1564069
   Google Scholar
• Vaz-Silva, W., Maciel, N. M., Bastos, R. P., & Pombal, J. P. (2015). Revealing two new species of the Rhinella margaritifera species group (Anura, Bufonidae): An enigmatic taxonomic group of Neotropical toads. Herpetologica, 71(3), 212–222. https://doi.org/10.1655/HERPETOLOGICA-D-14-00039
   Web of Science ®Google Scholar
• Vélez-Rodriguez, C. M., & Ruiz-Carranza, P. M. (2002). A new species of Bufo (Anura: Bufonidae) from Colombia. Herpetologica, 58, 453–462.
   Web of Science ®Google Scholar
• Walesiak, M., & Dudek, A. (2020). The choice of variable normalization method in cluster analysis. In K. S. Soliman (Ed.), Education excellence and innovation management: A 2025 vision to sustain economic development during global challenges (pp. 325–340). IBIMA.
   Google Scholar
• Yu, G., Rao, D., Matsui, M., & Yang, J. (2017). Coalescent-based delimitation outperforms distance-based methods for delineating less divergent species: the case of Kurixalus odontotarsus species group. Scientific Reports, 7(1), 16124. https://doi.org/10.1038/s41598-017-16309-1
   PubMedGoogle Scholar