Advanced search
Publication Cover
Nucleosides, Nucleotides & Nucleic Acids Volume 43, 2024 - Issue 7
Submit an article Journal homepage
131
Views
0
CrossRef citations to date
0
Altmetric
Research Article

“Difficult” deoxyribonucleotide sequences in the solid-phase synthesis by the phosphoramidite chemistry

Quanjian LiDepartment of Chemistry, Brock University, St. Catharines, ON, CanadaView further author information
&
Hongbin YanDepartment of Chemistry, Brock University, St. Catharines, ON, CanadaCorrespondence[email protected]
View further author information
Pages 655-663 | Received 29 Jun 2023, Accepted 11 Dec 2023, Published online: 20 Dec 2023

References

  • Kosuri, S.; Church, G. Large-Scale de Novo DNA Synthesis: Technologies and Applications. Nat. Methods. 2014, 11, 499–507. DOI: 10.1038/nmeth.2918.
  • Lönnberg, H. Synthesis of Oligonucleotides on a Soluble Support. Beilstein J. Org. Chem. 2017, 13, 1368–1387. DOI: 10.3762/bjoc.13.134.
  • Bayer, E.; Bleicher, K.; Maier, M. Improved Conditions for Solid Phase Synthesis of Oligonucleotides on PS-PEG Copolymers. Z. Naturforsch. B. 1995, 50, 1096–1100. DOI: 10.1515/znb-1995-0720.
  • Hachmann, J. P.; Lebl, M. Synthesis of Poly d(G-C) Oligonucleotides. Nucleosides. Nucleotides Nucl Acids. 2006, 25, 705–717. DOI: 10.1080/15257770600725903.
  • Hoose, A.; Vellacott, R.; Storch, M.; Freemont, P. S.; Ryadnov, M. G. DNA Synthesis Technologies to Close the Gene Writing Gap. Nat. Rev. Chem. 2023, 7, 144–161. DOI: 10.1038/s41570-022-00456-9.
  • Sun, J.; Joshi, D.; Betancourt, F.; Solodinin, A.; Woodland, B.; Yan, H. Anion Exchange Chromatography of Oligonucleotides under Denaturing Conditions. Nucleosides. Nucleotides Nucleic Acids. 2020, 39, 818–828. DOI: 10.1080/15257770.2019.1706096.
  • McAdorey, A.; Bennett, H.; Vanloon, J.; Yan, H. Use of Anion-Exchange (AEX) HPLC to Study Nucleic Acid Polymorphism. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2021, 1180, 122890. DOI: 10.1016/j.jchromb.2021.122890.
  • Septak, M. Kinetic Studies on Depurination and Detritylation of CPG-Bound Intermediates during Oligonucleotide Synthesis. Nucleic Acids Res. 1996, 24, 3053–3058. DOI: 10.1093/nar/24.15.3053.
  • LeProust, E. M.; Peck, B. J.; Spirin, K.; McCuen, H. B.; Moore, B.; Namsaraev, E.; Caruthers, M. H. Synthesis of High-Quality Libraries of Long (150mer) Oligonucleotides by a Novel Depurination Controlled Process. Nucleic Acids Res. 2010, 38, 2522–2540. DOI: 10.1093/nar/gkq163.
  • Thayer, J. R.; Murugaiah, V.; Wu, Y. Purity Analysis and Impurities Determination by AEX-HPLC. In Handbook of Analysis of Oligonucleotides and Related Products. Bonilla J, Srivatsa GS (Eds); Boca Raton, CRC Press, 2011.
  • Thayer, J. R.; Barreto, V.; Rao, S.; Pohl, C. Control of Oligonucleotide Retention on a pH-Stabilized Strong Anion Exchange Column. Anal. Biochem. 2005, 338, 39–47. DOI: 10.1016/j.ab.2004.11.013.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.