Postsynthetic Transformations of Oligodeoxynucleotides Originated at 6-Methylthio-purine Site

References

• Adamiak , R. W. , Biała , E. , Grześkowiak , K. , Kierzek , R. , Kraszewski , A. , Markiewicz , W. T. , Okupniak , J. , Stawiński , J. and Wiewiórowski , M. 1978 . Nucleic Acids Res. , 5 : 1889
   PubMed Web of Science ®Google Scholar
• Świderski , P. , Antkowiak , W. Z. and Adamiak , R. W. 1991 . Nucleosides & Nucleotides , 10 : 589
   Google Scholar
• Skalski , B. , Bartoszewicz , J. , Paszyc , S. , Gdaniec , Z. and Adamiak , R. W. 1987 . Tetrahedron , 43 : 3955
   Web of Science ®Google Scholar
• Mielewczyk , S. , Dominiak , G. , Gdaniec , Z. , Krzymańska-Olejnik , E. , Adamiak , R. W. and Skalski , B. 1991 . Nucleosides & Nucleotides , 10 : 263
   Web of Science ®Google Scholar
• Adamiak , R. W. , Biała , E. and Skalski , B. 1985 . Nucleic Acids Res. , 13 : 2989
   PubMed Web of Science ®Google Scholar
• 31P NMR spectra (CDCL3, δ ppm): −151.15, 150.35. HPLC. Waters 600E instrument equipped with diode-array detector and fluorescence detector was used in this studies. For the initial purification of 5′-O-DMTr oligos: NovaPak C18 cartridge, 25–50% acetonitrile gradient in 0.1M ammonium acetate; deprotected oligos were purified to homogeneity on μBondaPak C18 column in 5–15% acetonitrile gradient in the same buffer.
   Google Scholar
• Swiderski , P. , Dembek , P. , Antkowiak , W. Z. , Biała , E. and Adamiak , R. W. 1987 . Nucleic Acid Symph. Ser. , No. 18 : 105
   Google Scholar
• TABLE. HPLC (Waters, NovaPak C18 column, flow 0.8 ml/min) retention times data [min] for oligomers studied using specified acetonitrile gradient in 0.1M ammonium acetate buffer. Deoxy-oligomer a: × = MeS b: × = MeS− > O c: × = Pyr+ 1: T(6X-Pu)T 18.2 14.7 19.5 2: C(6X-Pu)C 13.1 6.8, 7.8 14.3 3: G(6X-Pu)G 17.2 14.6 17.9 4: A(6X-Pu)A 17.5 14.7, 15.1 18.6 5: (6X-Pu)T5 17.3 6.7 7.6 6: CTCCT(6X-Pu)TCCCT 16.6 15.9 16.7 Oligomers 1–4, gradient: 7–14% for 12[min], then isocratic. Oligomers 5, gradient: 5–15% for 12[min], then isocratic. Oligomers 6, gradient: 7–20% 12[min], then isocratic.
   Google Scholar
• Skalski , B. , Paszyc , S. , Adamiak , R. W. , Steer , R. P. and Verrall , R. E. 1989 . J. Chem. Soc. Perkin Trans. II , 1691
   Google Scholar
• Burdzy , A. , Skalski , B. , Paszyc , S. , Gdaniec , Z. , Popenda , M. and Adamiak , R. W. manuscript in preparation
   Google Scholar
• Skalski , B. , Steer , R. P. and Verrall , R. E. 1991 . J. Am. Chem. Soc. , 113 : 1756
   Google Scholar