Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytogenotoxicity activities of Combretum leprosum Mart. (Combretaceae)

References

• Ahmed, A. S., L. J. McGaw, E. E. Elgorashi, V. Naidoo, and J. N. Eloff. 2014. Polarity of extracts and fractions of four Combretum (Combretaceae) species used to treat infections and gastrointestinal disorders in southern African traditional medicine has a major effect on different relevant in vitro activities. J Ethnopharmacol 154:339–50. doi:10.1016/j.jep.2014.03.030.
   PubMed Web of Science ®Google Scholar
• Akeem, A. A., U. C. Ejikeme, and E. U. Okarafor. 2012. Antibacterial potentials of the ethanolic extract of the stem bark of Combretum micranthum G. Don and its fractions. J. Plant Stud. 1:75. doi:10.5539/jps.v1n2p75.
   Google Scholar
• Alves Filho, F. D. C., P. M. D. S. Cavalcanti, R. D. C. A. T. Passaglia, and G. Ballejo. 2015. Long-lasting endothelium-dependent relaxation of isolated arteries caused by an extract from the bark of Combretum leprosum. Einstein (Sao Paulo) 13:395–403. doi:10.1590/s1679-45082015ao3242.
   PubMed Web of Science ®Google Scholar
• Araujo-Espino, D. I., A. L. Zamora-Perez, G. M. Zúñiga-González, R. Gutiérrez-Hernández, G. Morales-Velazquez, and B. P. Lazalde-Ramos. 2017. Genotoxic and cytotoxic evaluation of Jatropha dioica Sessé ex Cerv. by the micronucleus test in mouse peripheral blood. Regul. Toxicol. Pharmacol. 86:260–64. doi:10.1016/j.yrtph.2017.03.017.
   PubMed Web of Science ®Google Scholar
• Ayres, M. C. C., M. H. Chaves, D. Rinaldo, W. Vilegas, and G. M. Vieira Júnior. 2008. Chemical constituents and antioxidant activity from leaves extracts of Terminalia fagifolia Mart. et Zucc. Quim. Nova 32:1509–12. doi:10.1590/S0100-40422009000600028.
   Web of Science ®Google Scholar
• Bannister, L. A., R. R. Mantha, Y. Devantier, E. S. Petoukhov, C. L. Brideau, M. L. Serran, and D. Y. Klokov. 2016. Dose and radioadaptive response analysis of micronucleus induction in mouse bone marrow. Int. J. Mol. Sci. 17 (9):1548. doi:10.3390/ijms17091548.
   Web of Science ®Google Scholar
• Barros, B. R. S., B. R. Barboza, B. A. Ramos, M. C. Moura, L. C. B. B. Coelho, T. H. Napoleão, M. T. S. Correia, P. M. G. Paiva, I. J. D. Cruz Filho, T. D. D. Silva, et al. 2019. Saline extract from Malpighia emarginata DC leaves showed higher polyphenol presence, antioxidant and antifungal activity and promoted cell proliferation in mice splenocytes. Ann. Acad. Bras. Cienc. 91:e20190916. doi:10.1590/0001-3765201920180358.
   PubMed Web of Science ®Google Scholar
• Bianchi, J., M. S. Mantovani, and M. A. Marin-Morales. 2015. Analysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture. J. Environ. Sci. (China) 36:102–11. doi:10.1016/j.jes.2015.03.034.
   PubMed Web of Science ®Google Scholar
• Bianchi, J., T. C. Fernandes, and M. A. Marin-Morales. 2016. Induction of mitotic and chromosomal abnormalities on Allium cepa cells by pesticides imidacloprid and sulfentrazone and the mixture of them. Chemosphere 144:475–83. doi:10.1016/j.chemosphere.2015.09.021.
   PubMed Web of Science ®Google Scholar
• Boroski, M., J. V. Visentainer, S. M. Cottica, and D. R. Morais. 2015. Antioxidants: Principles and Analytical Methods. 1nd ed. Curitiba, Brasil: Appris.
   Google Scholar
• Bueno, A. M. S., C. A. B. Pereira, and M. N. Rabello-Gay. 2000. Environmental genotoxicity evaluation using cytogenetic end points in wild rodents. Environ. Health. Perspect. 108:1165–69. doi:10.1289/ehp.108-1240198.
   PubMed Web of Science ®Google Scholar
• Burman, S., K. Bhattacharya, D. Mukherjee, and G. Chandra. 2018. Antibacterial efficacy of leaf extracts of Combretum album Pers. against some pathogenic bacteria. BMC Complement Altern. Med. 18 (1):213. doi:10.1186/s12906-018-2271-0.
   PubMed Web of Science ®Google Scholar
• Casassa, L. F., R. C. Larsen, and J. F. Harbertson. 2016. Effects of vineyard and winemaking practices impacting berry size on evolution of phenolics during winemaking. Am. J. Enol. Vitic. 67 (3):257–68. doi:10.5344/ajev.2016.15105.
   Web of Science ®Google Scholar
• Chaves, M. H. 1997. Analysis of extracts of plants by TLC: A methodology applied in the “organic chemistry” discipline. Quim. Nova 20:560–62. doi:10.1590/S0100-40421997000500019.
   Web of Science ®Google Scholar
• Chigayo, K., P. E. L. Mojapelo, S. Mnyakeni-Moleele, and J. M. Misihairabgwi. 2016. Phytochemical and antioxidant properties of different solvent extracts of Kirkia wilmsii tubers. Asian Pac. J. Trop. Biomed. 6:1037–43. doi:10.1016/j.apjtb.2016.10.004.
   Web of Science ®Google Scholar
• Chukwujekwu, J. C., and J. Van Staden. 2014. Cytotoxic and genotoxic effects of water extract of Distephanus angulifolius on Allium cepa Linn. S. Afr. J. Bot. 92:147–50. doi:10.1016/j.sajb.2014.03.001.
   Web of Science ®Google Scholar
• Cosmulescu, S., I. Trandafir, and V. Nour. 2017. Phenolic acids and flavonoids profiles of extracts from edible wild fruits and their antioxidant properties. Int. J. Food. Prop. 20:3124–34. doi:10.1080/10942912.2016.1274906.
   Web of Science ®Google Scholar
• Costa, D. A., M. H. Chaves, W. C. S. Silva, and C. L. S. Costa. 2010. Chemical constituents, total phenolics and antioxidant activity of Sterculia striata St. Hil. et Naudin. Acta. Amaz. 40:207–12. doi:10.1590/S0044-59672010000100026.
   Google Scholar
• Coulibaly, A. Y., R. Hashim, S. F. Sulaiman, O. Sulaiman, L. Z. Ang, and K. L. Ooi. 2014. Bioprospecting medicinal plants for antioxidant components. Asian Pac. J. Trop. Med. 9:S553–S559. doi:10.1016/S1995-7645(14)60289-3.
   Google Scholar
• Damiani, A. P., M. L. Garcez, L. Letieli de Abreu, T. H. Tavares, C. R. Boeck, and V. Moraes de Andrade. 2017. A reduction in DNA damage in neural tissue and peripheral blood of old mice treated with caffeine. J Toxicol Environ Health A 80:621–29. doi:10.1080/15287394.2017.1286901.
   Web of Science ®Google Scholar
• Dar, M. S., G. N. Joo, and S. A. Ganaie. 2018. Medicinal plants used by tribal and rural community of Sata Pokhran area of tehsil Pampore of district Pulwama, Jammu and Kashmir-India. J. Phytopharm. 7:66–71.
   Google Scholar
• Della-Pace, I. D., L. M. Rambo, L. R. Ribeiro, A. L. Saraiva, S. M. de Oliveira, C. R. Silva, J. G. Villarinho, M. F. Rossato, J. Ferreira, L. M. de Carvalho, et al. 2013. Triterpene 3β, 6β, 16β trihidroxilup-20(29)-ene protects against excitability and oxidative damage induced by pentylenetetrazol: The role of Na(+),K(+)-ATPase activity. Neuropharmacology 67:455–64. doi:10.1016/j.neuropharm.2012.10.022.
   PubMed Web of Science ®Google Scholar
• Dey, T. B., S. Chakraborty, K. K. Jain, A. Sharma, and R. C. Kuhad. 2016. Antioxidant phenolics and their microbial production by submerged and solid state fermentation process: A review. Trends Food Sci. Technol. 53:60–74. doi:10.1016/j.tifs.2016.04.007.
   Web of Science ®Google Scholar
• Dias, W. L. F., E. P. Junior, M. Oliveira, Y. L. P. Barbosa, J. Silva, J. S. Júnior, P. M. Almeida, and F. A. Martins. 2019. Cytogenotoxic effect, phytochemical screening and antioxidant potential of Jatropha mollissima (Pohl) Baill leaves. S. Afr. J. Bot. 123:30–35. doi:10.1016/j.sajb.2019.02.007.
   Web of Science ®Google Scholar
• Do Nascimento, K. F., F. M. F. Moreira, J. Alencar Santos, C. A. L. Kassuya, J. H. R. Croda, C. A. L. Cardoso, M. D. C. Vieira, A. L. T. Góis Ruiz, M. Ann Foglio, J. E. de Carvalho, et al. 2018. Antioxidant, anti-inflammatory, antiproliferative and antimycobacterial activities of the essential oil of Psidium guineense Sw. and spathulenol. J. Ethnopharmacol. 210:351–58. doi:10.1016/j.jep.2017.08.030.
   PubMed Web of Science ®Google Scholar
• Do Nascimento-Neto, L. G., F. F. Evaristo, M. F. Alves, M. R. Albuquerque, H. S. Dos Santos, P. N. Bandeira, F. V. Arruda, and E. H. Teixeira. 2015. Effect of the triterpene 3β, 6β, 16β-trihydroxylup-20(29)-ene isolated from the leaves of Combretum leprosum Mart. on cutaneous wounds in mice. J. Ethnopharmacol 171:116–20. doi:10.1016/j.jep.2015.05.037.
   PubMed Web of Science ®Google Scholar
• Do Santos, R. C., A. V. de Souza, M. Andrade-Silva, C. A. L. Kassuya, C. A. L. Cardoso, M. D. C. Vieira, and A. S. N. Formagio. 2018. Antioxidant, anti-rheumatic and anti-inflammatory investigation of extract and dicentrinone from Duguetia furfuracea (A. St.-Hil.) Benth. & Hook. f. J. Ethnopharmacol 211:9–16. doi:10.1016/j.jep.2017.09.019.
   PubMed Web of Science ®Google Scholar
• Dobrzyńska, M. M., A. Gajowik, and J. Radzikowska. 2016. The effect of in vivo resveratrol supplementation in irradiated mice on the induction of micronuclei in peripheral blood and bone marrow reticulocytes. Mutagenesis 31 (4):393–99. doi:10.1093/mutage/gev084.
   PubMed Web of Science ®Google Scholar
• Dutra, R. C., M. M. Campos, A. R. Santos, and J. B. Calixto. 2016. Medicinal plants in Brazil: Pharmacological studies, drug discovery, challenges and perspectives. Pharmacol. Res. 112:4–29. doi:10.1016/j.phrs.2016.01.021.
   PubMed Web of Science ®Google Scholar
• Facundo, V. A., C. H. S. Andrade, E. R. Silveira, R. Braz-Filho, and C. D. Hufford. 1993. Triterpenes and flavonoids from Combretum leprosum. Phytochemistry 32: 411–415. doi:10.1016/S0031-9422(00)95005-2
   Google Scholar
• Facundo, V. A., C. H. S. Andrade, E. R. Silveira, R. Braz-Filho, and C. D. Hufford. 1993. Triterpenes and flavonoids from Combretum leprosum.. Phytochemistry 32:411–15. doi:10.1016/S0031-9422(00)95005-2.
   Web of Science ®Google Scholar
• Facundo, V. A., K. A. Rios, C. M. Medeiros, J. S. L. T. Militão, A. L. P. Miranda, R. A. Epifanio, M. P. Carvalho, A. T. Andrade, A. C. PintoI, and C. M. Rezende. 2005. Arjunolic acid in the ethanolic extract of Combretum leprosum root and its use as a potential multi-functional phytomedicine and drug for neurodegenerative disorders: Anti-inflammatory and anticholinesterasic activities. J. Brazil Chem. Soc. 16:1309–12. doi:10.1590/S0103-50532005000800002.
   Web of Science ®Google Scholar
• Fawole, O. A., and U. L. Opara. 2016. Stability of total phenolic concentration and antioxidant capacity of extracts from pomegranate co-products subjected to in vitro digestion. BMC Complement Altern. Med. 16 (1):358. doi:10.1186/s12906-016-1343-2.
   PubMedGoogle Scholar
• Freire, J. S., B. C. S. Fernandes, J. A. C. Silva, J. R. S. Araujo, P. M. Almeida, J. S. Costa Jr, J. N. Silva, S. D. L. Freitas, and F. A. Martins. 2020. Phytochemical and antioxidant characterization, cytogenotoxicity, and antigenotoxicity of the fractions of the ethanolic extract of Poincianella bracteosa (Tul.) L.P. Quieroz. J. Toxicol. Environ. Health A 83:730–47. doi:10.1080/15287394.2020.1824136.
   PubMed Web of Science ®Google Scholar
• Freitas, K. S., L. A. Squarisi, N. O. Aceso, H. D. Nicolella, D. Ozelin, M. R. S. melo, A. P. P. Guissone, G. Fernandes, L. M. Silva, A. A. S. Filho, et al. 2020. Licochalcone A, a licorice flavonoid: Antioxidant, cytotoxic, genotoxic and chemopreventive potential. J. Toxicol. Environ. Health A 83:673–86. doi:10.1080/15287394.2020.1813228.
   Web of Science ®Google Scholar
• Garzón, G. A., C. E. Narváez, K. M. Riedl, and S. J. Schwartz. 2010. Chemical composition, anthocyanins, non-anthocyanin phenolics and antioxidant activity of wild bilberry (Vaccinium meridionale Swartz) from Colombia. Food Chem. 122:980–86. doi:10.1016/j.foodchem.2010.03.017.
   Web of Science ®Google Scholar
• Herrero, O., J. P. Martín, P. F. Freire, L. C. López, A. Peropadre, and M. J. Hazen. 2012. Toxicological evaluation of three contaminants of emerging concern by use of the Allium cepa test. Mutat. Res. 743:20–24. doi:10.1016/j.mrgentox.2011.12.028.
   PubMed Web of Science ®Google Scholar
• Hovhannisyan, G., T. Harutyunyan, and R. Aroutiounian. 2018. Micronuclei and what they can tell us in cytogenetic diagnostics. Curr. Genet. Med. Rep. 6:144–54. doi:10.1007/s40142-018-0149-6.
   Google Scholar
• Karou, D., M. H. Dicko, J. Simpore, and A. S. Traore. 2005. Antioxidant and antibacterial activities of polyphenols from ethnomedicinal plants of Burkina Faso. Afr. J. Biotechnol. 4:823–28.
   Web of Science ®Google Scholar
• Khalil, A. M., W. K. Salman, and K. M. Al-Qaoud. 2017. Evaluation of genotoxic potential of 2-(bromoacetamido) phenylboronic acid on balb/c mice peripheral blood cells using in vivo micronucleus assay. Jordan J. Pharm. Sci. 403:1–10.
   Google Scholar
• Koolen, H. H., F. M. da Silva, F. C. Gozzo, A. Q. de Souza, and A. D. de Souza. 2013. Antioxidant, antimicrobial activities and characterization of phenolic compounds from buriti (Mauritia flexuosa L. f.) by UPLC-ESI-MS/MS. Food Res. Int. 51:467–73. doi:10.1016/j.foodres.2013.01.039.
   Web of Science ®Google Scholar
• Lacouth-Silva, F., C. V. Xavier, S. Setúbal S. da, A. S. Pontes, N. M. Nery, O. B. de Castro, C. F. Fernandes, E. R. Honda, F. B. Zanchi, L. A. Calderon, et al. 2015. The effect of 3β, 6β, 16β-trihydroxylup-20(29)-ene lupane compound isolated from Combretum leprosum Mart. on peripheral blood mononuclear cells. BMC Complement Altern. Med 15:420. doi:10.1186/s12906-015-0948-1.
   PubMed Web of Science ®Google Scholar
• Leme, D. M., and M. A. Marin-Morales. 2009. Allium cepa test in environmental monitoring: A review on its application. Mutat. Res. 682:71–81. doi:10.1016/j.mrrev.2009.06.002.
   PubMed Web of Science ®Google Scholar
• Lira, S. R. D., R. N. Almeida, F. R. D. Almeida, F. D. Oliveira, and J. C. Duarte. 2002. Preliminary studies on the analgesic properties of the ethanol extract of Combretum leprosum.. Pharm. Biol. 682 (3):71–81. doi:10.1016/j.mrrev.2009.06.002.
   Google Scholar
• Liu, J., D. Guo, Y. Fan, J. Sun, J. Cheng, and Y. Shi. 2019. Experimental study on the antioxidant activity of Malus hupehensis (Pamp.) Rehd extracts in vitro and in vivo. J. Cell. Biochem. 120 (7):11878–89. doi:10.1002/jcb.28469.
   Web of Science ®Google Scholar
• Longhi-Balbinot, D. T., D. Lanznaster, C. H. Baggio, M. D. Silva, C. H. Cabrera, V. A. Facundo, and A. R. Santos. 2012. Anti-inflammatory effect of triterpene 3β, 6β, 16β-trihydroxylup-20(29)-ene obtained from Combretum leprosum Mart & Eich in mice. J. Ethnopharmacol. 142:59–64. doi:10.1016/j.jep.2012.04.013.
   PubMed Web of Science ®Google Scholar
• Lopes, L. S., R. B. Marques, S. S. Pereira, M. C. Ayres, M. H. Chaves, A. J. Cavalheiro, G. M. Vieira Júnior, and F. R. Almeida. 2010. Antinociceptive effect on mice of the hydroalcoholic fraction and (-) epicatechin obtained from Combretum leprosum Mart & Eich. Braz. J. Med. Biol. Res. 43:1184–92. doi:10.1590/s0100-879x2010007500121.
   PubMed Web of Science ®Google Scholar
• Lopes Lda, S., R. B. Marques, H. B. Fernandes, S. Pereira Sda, M. C. Ayres, M. H. Chaves, and F. R. Almeida. 2012. Mechanisms of the antinociceptive action of (-) epicatechin obtained from the hydroalcoholic fraction of Combretum leprosum Mart & Eic in rodents. J. Biomed. Sci. 19:68. doi:10.1186/1423-0127-19-68.
   PubMed Web of Science ®Google Scholar
• MacGregor, J. T., J. A. Heddle, M. Hite, B. H. Margolin, C. Ramel, M. F. Salamone, R. R. Tice, and D. Wild. 1987. Guidelines for the conduct of micronucleus assays in mammalian bone marrow erythrocytes. Mutat. Res. 189:103–12. doi:10.1016/0165-1218(87)90016-4.
   PubMed Web of Science ®Google Scholar
• Majolo, F., S. Bitencourt, B. Wissmann Monteiro, G. Viegas Haute, C. Alves, J. Silva, S. Pinteus, R. C. V. Santos, H. F. V. Torquato, E. J. Paredes-Gamero, et al. 2020. Antimicrobial and antileukemic effects: In vitro activity of Calyptranthes grandifolia aqueous leaf extract. J. Toxicol. Environ. Health A 83:289–301. doi:10.1080/15287394.2020.1753606.
   PubMed Web of Science ®Google Scholar
• Mareček, V., A. Mikyška, D. Hampel, P. Čejka, J. Neuwirthová, A. Malachová, and R. Cerkal. 2016. ABTS and DPPH methods as a tool for studying antioxidant capacity of spring barley and malt. J. Cereal Sci. 73:40–45. doi:10.1016/j.jcs.2016.11.004.
   Web of Science ®Google Scholar
• Martinez-Morales, F., A. J. Alonso-Castro, J. R. Zapata-Morales, C. Carranza-Álvarez, and O. H. Aragon-Martinez. 2020. Use of standardized units for a correct interpretation of IC50 values obtained from the inhibition of the DPPH radical by natural antioxidants. Chem. Pap 74:3325–34. doi:10.1007/s11696-020-01161-x.
   Web of Science ®Google Scholar
• Matos, F. D. A. 2009. Introduction to Experimental Phytochemistry. 3nd ed. Edições UFC.
   Google Scholar
• Md Zin, S. R., Z. Mohamed, M. A. Alshawsh, W. F. Wong, and N. M. Kassim. 2018. Mutagenicity evaluation of Anastatica hierochuntica L. aqueous extract in vitro and in vivo. Exp. Biol. Med 243: Maywood: 375–85. doi:10.1177/1535370217748574.
   Web of Science ®Google Scholar
• Meda, A., C. E. Lamien, M. Romito, J. Millogo, and O. G. Nacoulma. 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem. 91:571–77. doi:10.1016/j.foodchem.2004.10.006.
   Web of Science ®Google Scholar
• Mendonça, E. D., J. da Silva, M. S. Dos Santos, P. Carvalho, D. K. Papke, C. F. Ortmann, J. N. Picada, F. H. Reginatto, and A. de Barros Falcão Ferraz. 2016. Genotoxic, mutagenic and antigenotoxic effects of Cecropia pachystachya Trécul aqueous extract using in vivo and in vitro assays. J. Ethnopharmacol. 193:214–20. doi:10.1016/j.jep.2016.07.046.
   PubMed Web of Science ®Google Scholar
• Menezes, A. P., J. da Silva, C. Fisher, F. R. da Silva, J. M. Reyes, J. N. Picada, A. G. Ferraz, D. S. Corrêa, S. M. Premoli, J. F. Dias, et al. 2016. Chemical and toxicological effects of medicinal Baccharis trimera extract from coal burning area. Chemosphere 146:396–404. doi:10.1016/j.chemosphere.2015.12.028.
   PubMed Web of Science ®Google Scholar
• Merino, F. J. Z., V. B. Oliveira, C. S. Paula, F. C. Cansian, A. M. Souza, M. Zuchetto, B. C. K. Hirota, A. F. S. Duarte, J. D. Kulik, M. D. Miguel, et al. 2015. Phytochemical analysis, antioxidant potential and toxicity of crude ethanol extract and fractions of the species Senecio westermanii Dusén against Artemia salina. Rev. Bras. Plantas Med 17:1031–40. doi:10.1590/1983-084X/14_137.
   Google Scholar
• Miaffo, D., S. L. Wansi, F. Ntchapda, and A. Kamanyi. 2020. Chronic oral safety study of the aqueous extract of Combretum molle twigs on biochemical, haematological and antioxidant parameters of Wistar rats. BMC Complement Med. Ther. 20:1–9. doi:10.1186/s12906-020-02896-6.
   PubMedGoogle Scholar
• Migliore, L., F. Coppedè, M. Fenech, and P. Thomas. 2011. Association of micronucleus frequency with neurodegenerative diseases. Mutagenesis 26 (1):85–92. doi:10.1093/mutage/geq067.
   PubMed Web of Science ®Google Scholar
• Moraes, L. S., B. Z. Rohor, L. B. Areal, E. V. Pereira, A. M. Santos, V. A. Facundo, A. R. Santos, R. G. Pires, and C. Martins-Silva. 2016. Medicinal plant Combretum leprosum Mart ameliorates motor, biochemical and molecular alterations in a Parkinson’s disease model induced by MPTP. J. Ethnopharmacol. 185:68–76. doi:10.1016/j.jep.2016.03.041.
   PubMed Web of Science ®Google Scholar
• Mtunzi, F. M., I. P. Ejidike, I. Ledwaba, A. Ahmed, V. E. Pakade, M. J. Klink, and S. J. Modise. 2017. Solvent-solvent fractionations of Combretum erythrophyllum (Burch.) leave extract: Studies of their antibacterial, antifungal, antioxidant and cytotoxicity potentials. Asian Pac. J. Trop. Med. 10:670–79. doi:10.1016/j.apjtm.2017.07.007.
   PubMed Web of Science ®Google Scholar
• Muddathir, A. M., K. Yamauchi, I. Batubara, E. A. M. Mohieldin, and T. Mitsunaga. 2017. Anti-tyrosinase, total phenolic content and antioxidant activity of selected Sudanese medicinal plants. S. Afr. J. Bot. 109:9–15. doi:10.1016/j.sajb.2016.12.013.
   Web of Science ®Google Scholar
• Mushi, N. F., Z. H. Mbwambo, E. Innocent, and S. Tewtrakul. 2012. Antibacterial, anti-HIV-1 protease and cytotoxic activities of aqueous ethanolic extracts from Combretum adenogonium Steud. Ex A. Rich (Combretaceae). BMC Complement Altern. Med. 12 (1):163. doi:10.1186/1472-6882-12-163.
   PubMedGoogle Scholar
• Nunes, P. H., M. D. C. C. Martins, R. D. C. M. Oliveira, M. H. Chaves, E. A. Sousa, J. R. Leite, L. M. Véras, and F. R. Almeida. 2014. Gastric antiulcerogenic and hypokinetic activities of Terminalia fagifolia Mart. & Zucc. (Combretaceae). Biomed Res. Int. 2014:261745. doi:10.1155/2014/261745.
   PubMed Web of Science ®Google Scholar
• Odeh, I., F. Al-Rimawi, J. Abbadi, L. Obeyat, M. Qabbajeh, and A. Hroub. 2014. Effect of harvesting date and variety of date palm on antioxidant capacity, phenolic and flavonoid content of date palm (Phoenix Dactylifera). J. Food Nutr. Res. 2:499–505. doi:10.12691/jfnr-2-8-11.
   Google Scholar
• OECD. 2016. Test Nº 474: Mammalian Erythrocyte Micronucleus Test, OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris: OECD Publishing. 10.1787/9789264264762-en.
   Google Scholar
• Olaoluwa, O. O., and F. Ogunbor. 2015. Phytochemical screening, antimicrobial properties and essential oil constituents of Combretum sordidum exell. Int. J. Pharm. Sci. Res. 6:1176. doi:10.13040/IJPSR.0975-8232.6(3).1176-80.
   Web of Science ®Google Scholar
• Onofre, S. B., D. Abatti, A. A. Tessaro, and A. B. Tessaro. 2016. Total phenolic, flavonoid content and antioxidant activity of Vitex megapotamica (Spreng.) Moldenke. CeN 383:1197–204. doi:10.5902/2179460X21363.
   Google Scholar
• Pietrovski, E. F., K. A. Rosa, V. A. Facundo, K. Rios, M. C. Marques, and A. R. Santos. 2006. Antinociceptive properties of the ethanolic extract and of the triterpene 3beta,6beta,16beta-trihidroxilup-20(29)-ene obtained from the flowers of Combretum leprosum in mice. Pharmacol. Biochem. Behav. 83:90–99. doi:10.1016/j.pbb.2005.12.010.
   PubMed Web of Science ®Google Scholar
• Prajitha, V., and J. E. Thoppil. 2016. Genotoxic and antigenotoxic potential of the aqueous leaf extracts of Amaranthus spinosus Linn. using Allium cepa assay. S. Afr. J. Bot. 102:18–25. doi:10.1016/j.sajb.2015.06.018.
   Web of Science ®Google Scholar
• Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans. 1998. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 72:1231–37. doi:10.1016/S0891-5849(98)00315-3.
   Google Scholar
• Ribeiro, J. C., S. F. Andrade, J. K. Bastos, and E. L. Maistro. 2009. Evaluation of the genotoxic potential of Austroplenckia populnea (Reiss) Lundell chloroform fraction from barkwood extract in rodent cells in vivo. Braz. J. Biol 69:1141–47. doi:10.1590/s1519-69842009000500019.
   PubMed Web of Science ®Google Scholar
• Roberto, M. M., C. M. Jamal, O. Malaspina, and M. A. Marin-Morales. 2016. Antigenotoxicity and antimutagenicity of ethanolic extracts of Brazilian green propolis and its main botanical source determined by the Allium cepa test system. Genet. Mol. Biol. 39:257–69. doi:10.1590/1678-4685-gmb-2015-0130.
   PubMed Web of Science ®Google Scholar
• Rody, H. V. S., D. D. C. Gontijo, V. P. M. Coelho, M. C. Ventrella, R. M. Pádua, L. G. Fietto, and J. P. V. Leite. 2018. Mutagenic activity and chemical composition of phenolic-rich extracts of leaves from two species of Ficus medicinal plants. J. Toxicol. Environ. Health A 81:861–72. doi:10.1080/15287394.2018.1498420.
   PubMed Web of Science ®Google Scholar
• Samuelsson, G., and L. Bohlin. 2017. Drugs of Natural Origin: A Treatise of Pharmacognosy. 7 ed. CRC Press Inc.
   Google Scholar
• Santos, C. C. S., C. C. Guilhon, D. S. A. Moreno, C. S. Alviano, C. D. S. Estevam, A. F. Blank, and P. D. Fernandes. 2018. Anti-inflammatory, antinociceptive and antioxidant properties of Schinopsis brasiliensis bark. J. Ethnopharmacol. 213:176–82. doi:10.1016/j.jep.2017.11.012.
   PubMed Web of Science ®Google Scholar
• Sbhatu, D. B., and H. B. Abraha. 2020. Preliminary antimicrobial profile of Solanum incanum L.: A common medicinal plant. Evid. Based Complement Altern. Med. 2020:3647065. doi:10.1155/2020/3647065.
   PubMed Web of Science ®Google Scholar
• Silva, D. S. B. S., B. Barboza, A. C. F. S. Garcia, B. de Oliveira, C. S. Estevam, V. A. Neto, A. L. L. M. Santos, A. S. Dias, R. ScherI, and S. M. Pantaleao. 2013. Investigation of protective effects of Erythrina velutina extract against MMS induced damages in the root meristem cells of Allium cepa. Rev. Bras. Farmacogn. 23:273–78. doi:10.1590/S0102-695X2013005000006.
   Google Scholar
• Silva, J. D. N., N. B. N. Monção, R. R. S. de Farias, M. H. Amdgl Citó, M. R. S. Chaves, D. J. B. Araújo, L. C. Pessoa, A. Lima, G. C. G. Ecdc Araújo, M. P. D. Militão, et al. 2020. Toxicological, chemopreventive, and cytotoxic potentialities of rare vegetal species and supporting findings for the Brazilian Unified Health System (SUS). J. Toxicol. Environ. Health A 83:525–45. doi:10.1080/15287394.2020.1780658.
   PubMed Web of Science ®Google Scholar
• Silva, M. T. O., M. A. C. Maia, M. D. C. D. Silva, T. M. Torres, J. C. D. S. Pereira, J. R. C. Araújo, R. E. D. Silva, A. L. D. C. Souza, T. N. Barbosa, and A. C. D. S. Bezerra. 2018. Anthelmintic effect of Cassia fistula and Combretum leprosum protein fractions against goat gastrointestinal nematodes. Rev. Bras. Parasitol. Vet. 27:237–41. doi:10.1590/s1984-296120180041.
   PubMed Web of Science ®Google Scholar
• Silva, R. M., L. D. Pereira, J. H. Véras, C. R. Vale, L. Chen-Chen, and S. D. Santos. 2016. Protective effect and induction of DNA repair by Myrciaria cauliflora seed extract and pedunculagin on cyclophosphamide-induced genotoxicity. Mutat. Res. 810:40–47. doi:10.1016/j.mrgentox.2016.10.001.
   PubMed Web of Science ®Google Scholar
• Tedesco, M., A. W. Kuhn, V. D. Frescura, A. A. Boligon, M. L. Athayde, S. B. Tedesco, and A. Silva. 2017. Assessment of the antiproliferative and antigenotoxic activity and phytochemical screening of aqueous extracts of Sambucus australis Cham. & Schltdl. (ADOXACEAE). Ann. Acad. Bras. Cienc. 89:2141–54. doi:10.1590/0001-3765201720150138.
   PubMed Web of Science ®Google Scholar
• Thomford, N. E., K. Dzobo, D. Chopera, A. Wonkam, M. Skelton, D. Blackhurst, S. Chirikure, and C. Dandara. 2015. Pharmacogenomics implications of using herbal medicinal plants on African populations in health transition. Pharmaceuticals (Basel) 8 (3):637–63. doi:10.3390/ph8030637.
   PubMedGoogle Scholar
• Timothy, O., M. Idu, D. I. Olorunfemi, and O. Ovuakporie-Uvo. 2014. Cytotoxic and genotoxic properties of leaf extract of Iacina trichantha Oliv. S. Afr. J. Bot. 91:71–74. doi:10.1016/j.sajb.2013.11.008.
   Web of Science ®Google Scholar
• Uchôa, V. T., C. M. M. Sousa, A. A. Carvalho, A. E. G. Sant’Ana, and M. H. Chaves. 2016. Free radical scavenging ability of Ximenia americana L. stem bark and leaf extracts. J. Appl. Pharm. Sci 6:091–096. doi:10.7324/JAPS.2016.60213.
   Google Scholar
• Viau, C. M., D. J. Moura, P. Pflüger, V. A. Facundo, and J. Saffi. 2016. Structural aspects of antioxidant and genotoxic activities of two flavonoids obtained from ethanolic extract of Combretum leprosum. Evid. Based Complement Alternat.. Med 2016:9849134. doi:10.1155/2016/9849134.
   Web of Science ®Google Scholar
• Viau, C. M., D. J. Moura, V. A. Facundo, and J. Saffi. 2014. The natural triterpene 3β,6β,16β-trihydroxy-lup-20(29)-ene obtained from the flowers of Combretum leprosum induces apoptosis in MCF-7 breast cancer cells. BMC Complement Altern. Med. 14:280. doi:10.1186/1472-6882-14-280.
   PubMed Web of Science ®Google Scholar
• Wakata, A., Y. Miyamae, S. Sato, T. Suzuki, T. Morita, N. Asano, T. Awogi, K. Kondo, and M. Hayashi. 1998. Evaluation of the rat micronucleus test with bone marrow and peripheral blood: Summary of the 9th collaborative study by CSGMT/JEMS. MMS. Collaborative study group for the micronucleus test. Environmental mutagen society of Japan. Mammalian mutagenicity study group. Environ. Mol. Mutagen. 32:84–100. doi:10.1002/(SICI)1098-2280(1998)32:1<84::AID-EM10>3.0.CO;2-K.
   PubMed Web of Science ®Google Scholar
• You, X., T. Ando, J. Xi, Y. Cao, W. Liu, X. Zhang, M. Honma, K. Masumura, and Y. Luan. 2018. Gene mutation and micronucleus assays in gpt delta mice treated with 2,2ʹ,4,4ʹ-tetrabromodiphenyl ether. Mutagenesis 33:153–60. doi:10.1093/mutage/gey002.
   PubMed Web of Science ®Google Scholar
• Yuan, H., Q. Ma, L. Ye, and G. Piao. 2016. The traditional medicine and modern medicine from natural products. Molecules 21 (5):559. doi:10.3390/molecules21050559.
   PubMed Web of Science ®Google Scholar
• Yun, J. W., J. R. You, Y. S. Kim, S. H. Kim, E. Y. Cho, J. H. Yoon, E. Kwon, J. J. Jang, J. S. Park, H. C. Kim, et al. 2018. In vitro and in vivo safety studies of cinnamon extract (Cinnamomum cassia) on general and genetic toxicology. Regul. Toxicol. Pharmacol. 95:115–23. doi:10.1016/j.yrtph.2018.02.017.
   PubMed Web of Science ®Google Scholar
• Zakaria, N. N. A., E. J. Okello, M. J. Howes, M. A. Birch-Machin, and A. Bowman. 2018. In vitro protective effects of an aqueous extract of Clitoria ternatea L. flower against hydrogen peroxide-induced cytotoxicity and UV-induced mtDNA damage in human keratinocytes. Phytother. Res. 32:1064–72. doi:10.1002/ptr.6045.
   PubMed Web of Science ®Google Scholar