117
Views
2
CrossRef citations to date
0
Altmetric
Research Articles

Cytogenotoxic evaluations of leaves and stems extracts of Rubus rosifolius in primary metabolically noncompetent cells

, , , , ORCID Icon, & show all

References

  • Alves, A. B. C. R., R. S. Santos, S. S. Calil, R. Niero, J. S. Lopes, F. F. Perazzo, P. C. P. Rosa, S. F. Andrade, V. Cechinel-Filho, and E. L. Maistro. 2014. Genotoxic assessment of Rubus imperialis (Rosaceae) extract in vivo and its potential chemoprevention against cyclophosphamide-induced DNA damage. J. Ethnophamacol 153 (3):694–700. doi:10.1016/j.jep.2014.03.033.
  • ANVISA. 2004. Agência Nacional de Vigilância Sanitária. Resolução nº 90, de 16 de março de 2004. Guia para a realização de estudos de toxicidade pré-clínica de fitoterápicos. Acesso em: 17 jul. 2020
  • Araldi, R. P., T. C. de Melo, T. B. Mendes, P. L. de Sá Júnior, B. H. Nozima, E. T. Ito, R. F. de Carvalho, E. B. de Souza R, de Cassia-Stocco, and R. de Cassia Stocco. 2015. Using the comet and micronucleus assays for genotoxicity studies: A review. Biomed. Pharmacother 72:74–82. doi:10.1016/j.biopha.2015.04.004.
  • Bagattoli, P. C., D. D. Cipriani, L. N. Mariano, M. Correa, T. M. Wagner, V. F. Noldin, V. Cechinel-Filho, R. Niero, and T. S. Almeida. 2016. Phytochemical, antioxidant and anticancer activities of extracts of seven fruits found in the southern Brazilian flora. Indian J. Pharm. Sci 78 (1):34–40. doi:10.4103/0250-474X.180239.
  • Bansal, H., V. S. S. Pravallika, G. Srivastava, and D. Ganjewala. 2022. Bioactivity assessment of essential oils of Cymbopogon species using a network pharmacology approach. Biol. Future 73 (1):107–18. doi:10.1007/s42977-022-00111-w.
  • Berté, P. E., J. da Silva-Lopes, N. G. Comandulli, D. W. Rangel, F. D. Monache, V. C. Filho, R. Niero, and S. F. de Andrade. 2014. Evaluation of the gastroprotective activity of the extracts, fractions, and pure compounds obtained from aerial parts of Rubus imperialis in different experimental models. Diff Exp. Models Naunyn. Schmiedebergs Arch. Pharmacol 387 (4):313–19. doi:10.1007/s00210-013-0954-0.
  • Booth, E. D., P. J. Rawlinson, P. Maria Fagundes, and K. A. Leiner. 2017. Regulatory requirements for genotoxicity assessment of plant protection product active ingredients, impurities, and metabolites. Environ. Mol. Mutagen. 58 (5):325–44. doi:10.1002/em.22084.
  • Burlinson, B. 2012. The in vitro and in vivo comet assays. Meth. Mol. Biol 817:143–63.
  • CNS. 1997. Conselho Nacional de Saúde, Resolução nº 251, 07/08/1997. Acesso em: 12 nov. 2020. http://conselho.saude.gov.br/docs/Resolucoes/Reso251.doc
  • Colalto, C. 2018. What phytotherapy needs: Evidence-based guidelines for better clinical practice. Phytother. Res 32 (3):413–25. doi:10.1002/ptr.5977.
  • Collins, A. R., A. G. Ma, and S. J. Duthie. 1995. The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells. Mutat. Res. 336 (1):69–77. doi:10.1016/0921-8777(94)00043-6.
  • Collins, A. R., A. A. Oscoz, G. Brunborg, I. Gaivão, L. Giovanelli, M. Kruszewski, C. C. Smith, and R. Stetina. 2008. The comet assay: Topical issues. Mutagenesis 23 (3):143–51. doi:10.1093/mutage/gem051.
  • Cragg, G. M., and D. J. Newman. 2013. Natural products: A continuing source of novel drug leads. Biochim. Biophys. Acta 1830 (6):3670–95. doi:10.1016/j.bbagen.2013.02.008.
  • da Fonsêca, D. V., C. S. M. Bezerra-Filho, T. C. Lima, R. N. de Almeida, and D. P. de Sousa. 2019. Anticonvulsant essential oils and their relationship with oxidative stress in epilepsy. Biomolecules 9 (12):835. doi:https://doi.org/10.3390/biom9120835.
  • Desmiaty, Y., E. Mulatsari, F. C. Saputri, M. Hanafi, R. Prastiwi, and B. Elya. 2020. Inhibition of pancreatic elastase in silico and in vitro by Rubus rosifolius leaves extract and its constituents. J. Pharm. Bioal. Sci 12 (3):317–23. doi:10.4103/jpbs.JPBS_271_19.
  • Falzon C C, and A. Balabanova. 2017. Phytotherapy: An introduction to herbal medicine. Prim. Care 44:217–27. doi:10.1016/j.pop.2017.02.001.
  • Fenech, M. 2000. The in vitro micronucleus technique. Mutat. Res. 455 (1–2):81–95. doi:10.1016/S0027-5107(00)00065-8.
  • Fenech, M. 2020. Cytokinesis-block micronucleus cytome assay evolution into a more comprehensive method to measure chromosomal instability. Genes 11 (10):1203. doi:https://doi.org/10.3390/genes11101203.
  • Fernandes, A. S., J. Hollanda Véras, L. S. Silva, S. C. Puga, E. F. Luiz Cardoso Bailão, M. G. de Oliveira, C. G. Cardoso, C. C. Carneiro, S. D. Costa Santos, and L. Chen-Chen. 2022. Pedunculagin isolated from Plinia cauliflora seeds exhibits genotoxic, antigenotoxic and cytotoxic effects in bacteria and human lymphocytes. J. Toxicol. Environ. Health Part A 85 (9):353–63. doi:10.1080/15287394.2021.2009947.
  • Finn, C., B. M. Yorgey, B. C. Strik, and R. Martin. 2008. New USDA-ARS blackberry cultivars bring diversity to the market. Acta Horticul. 777: IX Int Rubus and Ribes Symposium (777):8. doi:10.17660/ActaHortic.2008.777.8.
  • Gavanji, S., A. Bakhtari, A. C. Famurewa, and E. M. Othman. 2023. Cytotoxic activity of herbal medicines as assessed in vitro: A review. Chem. Biodivers. 20 (2):e202201098. doi:10.1002/cbdv.202201098.
  • George, B. P., P. Thangaraj, S. Thankarajan, and S. Shanmugam. 2014. Antitumor and wound healing properties of Rubus niveus Thunb. root. J. Environ. Pathol. Toxicol. Oncol 33 (2):145–58. doi:10.1615/JEnvironPatholToxicolOncol.2014010949.
  • Guo, X., J. E. Seo, X. Li, and N. Mei. 2020. Genetic toxicity assessment using liver cell models: Past, present, and future. J. Toxicol. Environ. Health B 23 (1):27–50. doi:10.1080/10937404.2019.1692744.
  • Kanegusuku, M., D. Sbors, E. S. Bastos, M. M. D. Souza, V. Cechinel-Filho, R. A. Yunes, F. D. Monache, and R. Niero. 2007. Phytochemical and analgesic activity of extract, fractions and a 19-hydroxyursane-type triterpenoid obtained from Rubus rosaefolius (Rosaceae). Biol. Pharm. Bull. 30 (5):999–1002. doi:10.1248/bpb.30.999.
  • Knasmüller, S., V. Mersch-Sundermann, S. Kevekordes, F. Darroudi, W. W. Huber, C. Hoelzl, J. Bichler, and B. J. Majer. 2004. Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants; current state of knowledge. Toxicology 198 (1–3):315–28. doi:10.1016/j.tox.2004.02.008.
  • Kreander, K., A. Galkin, S. Vuorela, P. Tammela, L. Laitinen, M. Heinonen, and P. Vuorela. 2006. In-vitro mutagenic potential and effect on permeability of co-administered drugs across Caco-2 cell monolayers of Rubus idaeus and its fortified fractions. J. Pharm. Pharmacol. 58 (11):1545–52. doi:10.1211/jpp.58.11.0016.
  • Lopes, K. S., H. G. Sousa, F. A. S. Filho, E. R. S. Neta, S. G. deLima, M. D. dos, S. Rocha, R. B. Marques, C. L. S. Costa, A. N. deOliveira, et al. 2022. Identification of bioactive compounds and cytogenotoxicity of the essential oil from the leaves of Croton heliotropiifolius Kunth. J. Toxicol. Environ Health 85 (24):1002–18. doi:10.1080/15287394.2022.2146618.
  • Mauro, C., C. M. Z. Cardoso, P. S. Lopes, E. M. C. Marcondes, J. P. Miranda, M. Frota, A. L. Pacheco, D. A. O. Arruda, M. Frota, and A. L. Pacheco. 2002. Estudo botânico, fitoquímico e avaliação da atividade antimicrobiana de R. rosifolius Sm., Rosaceae. Braz. J. Pharmacog 12:23–25. doi:10.1590/S0102-695X2002000300012.
  • Milošević-Djordjević, O., M. Radović-Jakovljević, A. Marković, M. Stanković, A. Ćirić, D. Marinković, and D. Grujičić. 2018. Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes. Turk J. Biol 42 (2):152–62. doi:10.3906/biy-1707-36.
  • Nesello, L. A. N., M. L. M. L. Beleza, M. Mariot, L. N. B. Mariano, P. de Souza, A. Campos, V. Cechinel-Filho, S. F. Andrade, and L. M. da Silva. 2017. Gastroprotective value of berries: Evidences from methanolic extracts of Morus nigra and Rubus niveus fruits. Gastroenterol Res. Pract 2017:7089697. doi:10.1155/2017/7089697.
  • OECD. 2016. Test No. 489: In Vivo Mammalian Alkaline Comet Assay, OECD Guidelines for the Testing of Chemicals, Section 4. Paris: OECD Publishing.
  • OECD, T. G. 487. 2016. OECD guideline for the testing of chemicals. In vitro mammalian cell micronucleus test. Avaiable at: https://www.oecd.org/chemicalsafety/test-no-487-in-vitro-mammalian-cell-micronucleus-test-9789264264861-en.htm
  • Ostrosky, E. A., E. M. Marcondes, S. O. Nishikawa, P. S. Lopes, G. H. C. Varca, T. D. J. A. Pinto, T. V. O. Consiglieri, A. R. Baby, M. V. R. Velasco, and T. M. Kaneko. 2011. Rubus rosifolius extract as a natural preservative candidate in topical formulations. Pharm. Sci. Technol 12 (2):732–37. doi:10.1208/s12249-011-9635-9.
  • Patel, A. V., J. Rojas, and C. G. Dacke. 2004. Therapeutic constituents and actions of Rubus species. Cur. Med. Chem 11 (11):1501–12. doi:10.2174/0929867043365143.
  • Pereira, E. D. M., J. Silva, P. S. Carvalho, I. Grivicich, J. N. Picada, I. B. Salgado-Júnior, G. J. Vasques, Pereira, F. H. Reginatto, and A. B. F. Ferraz. 2020. In vivo and in vitro toxicological evaluations of aqueous extract from Cecropia pachystachya leaves. J. Toxicol. Environ. Health Part A, 83 (19–20):659–71. doi:10.1080/15287394.2020.1811817.
  • Petreanu, M. 2017. Estudo fitoquímico eavaliação da ação biológica de três espécies da flora catarinense: Solanum capsicoides, Rubus rosifolius eMyroparpus frondosus. Tese de Doutorado. Programa de pós-Graduação em Ciências Farmacêuticas. Universidade do Vale do Itajai.
  • Petreanu, M., E. K. Ferreira, A. P. M. Sagaz, D. B. Vendramini-Costa, A. L. T. G. Ruiz, J. E. Carvalho, A. Campos, V. Cechinel-Filho, F. D. Monache, and R. Niero. 2015. Uncommon trimethoxylated flavonol obtained from Rubus rosifolius leaves and its antiproliferative activity. Evid. Based Complement Alternat. Med 2015:341216. doi:10.1155/2015/341216.
  • Pfuhler, S., M. Fellows, J. van-Benthem, R. Corvi, R. Curren, K. Dearfield, P. Fowler, R. Frötschl, A. Elhajouji, L. Le-Hégarat, et al. 2011. In vitro genotoxicity test approaches with better predictivity: summary of an IWGT workshop. Mutat. Res. 723 (2):101–07. doi:10.1016/j.mrgentox.2011.03.013.
  • Popović, Z., R. Matić, S. Bojović M, M. Stefanović, and V. Vidaković. 2016. Ethnobotany and herbal medicine in modern complementary and alternative medicine: An overview of publications in the field of I&C medicine 2001-2013. J. Ethnopharmacol 181:182–92. doi:10.1016/j.jep.2016.01.034.
  • Quadros, A. P. O., L. M. Almeida, M. Petreanu, R. Niero, P. C. P. Rosa, A. C. H. F. Sawaya, M. S. Mantovani, I. O. M. Gaivão, and E. L. Maistro. 2020. Risk assessment via genotoxicity, metabolism, apoptosis, and cell growth effects in a HepG2/C3A cell line upon treatment with Rubus rosifolius (Rosaceae) leaves extract. J. Toxicol Environ Health 83 (13–14):495–508. doi:10.1080/15287394.2020.1779888.
  • Quadros, A. P. O., B. Oshiiwa, M. Petreanu, R. Niero, P. C. P. Rosa, A. C. H. F. Sawaya, M. S. Mantovani, I. O. M. Gaivão, and E. L. Maistro. 2023. Rubus rosifolius (Rosaceae) stem extract induces cell injury and apoptosis in human hepatoma cell line. Toxicol. In Vitro 86:105485. doi:10.1016/j.tiv.2022.105485.
  • Rambaran, T. F., N. Nembhard, C. S. Bowen-Forbes, and R. L. Alexander-Lindo. 2020. Hypoglycemic effect of the fruit extracts of two varieties of Rubus rosifolius. J. Food Biochem. 44 (9):e13365. doi:10.1111/jfbc.13365.
  • Rocha, J. D., F. M. Carneiro, A. S. Fernandes, J. M. Morais, L. L. Borges, L. Chen-Chen, L. M. de Almeida, and E. F. L. C. Bailão. 2022. Toxic potential of cerrado plants on different organisms. Int. J Mol Sci 23 (7):3413. doi:10.3390/ijms23073413.
  • Saab, A. M., R. Gambari, G. Sacchetti, A. Guerrini, I. Lampronti, M. Tacchini, A. El-Samrani, S. Medawar, H. Makhlouf, M. Tannoury, et al. 2018. Phytochemical and pharmacological properties of essential oils from Cedrus species. Nat. Prod. Res. 32 (12):1415–27. doi:10.1080/14786419.2017.1346648.
  • Sem, T., and S. K. Samanta. 2015. Medicinal plants, human health and biodiversity: A broad review. Adv. Biochem. Eng. Biotechnol. 147:59–110. doi:10.1007/10_2014_273.
  • Shanaida, M., I. Jasicka-Misiak, E. Makowicz, N. Stanek, V. Shanaida, and P. P. Wieczorek. 2020. Development of high-performance thin layer chromatography method for identification of phenolic compounds and quantification of rosmarinic acid content in some species of the Lamiaceae family. J. Pharm. Bioallied Sci 12 (2):139–45. doi:10.4103/jpbs.JPBS_322_19.
  • Silva, J. D. N., N. B. N. Monção, R. R. S. de Farias, A. M. D. G. L. Citó, M. H. Chaves, M. R. S. Araújo, D. J. B. Lima, C. Pessoa, A. Lima, E. C. D. C. Araújo, et al. 2020. Toxicological, chemopreventive, and cytotoxic potentialities of rare vegetal species and supporting findings for the Brazilian Unified Health System (SUS). J. Toxicol. Environ. Health Part A 83 (13–14):525–45. doi:10.1080/15287394.2020.1780658.
  • Souza, P., T. Boeing, L. B. Somensi, C. C. Cechinel-Zanchett, J. K. Bastos, M. Petreanu, R. Niero, V. Cechinel-Filho, L. M. Silva, and S. F. Andrade. 2017. Diuretic affect of extracts, fraction and two compounds 2α,3β,19α-trihydroxy-urs-12-en-28-oic acid and 5-hydroxy-3,6,7,8,4ʹ-pentamethoxyflavone from Rubus rosifolius Sm. (Rosaceae) leaves in rats. Naunyn-Schmiedeberg’s Arch. Pharmacol (390):351–60. doi:10.1007/s00210-016-1333-4.
  • Strober, W. 2019. Trypan blue exclusion test of cell viability. Curr. Prot. Immunol 111 (1):A3.1–3.3. doi:10.1002/0471142735.ima03bs111.
  • Thomford, N. E., D. A. Senthebane, A. Rowe, D. Munro, P. Seele, A. Maroyi, and K. Dzobo. 2018. Natural products for drug discovery in the 21st Century: Innovations for novel drug discovery. Int. J. Mol Sci 19 (6):1578. doi:10.3390/ijms19061578.
  • Tice, R. R., E. Agurel, D. Anderson, B. Burlinson, A. Hartmann, H. Kobayashi, Y. Miyamae, E. Rojas, J. C. Ryu, and Y. F. Sasaki. 2000. Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35:206–21. doi:10.1002/(SICI)1098-2280(2000)35:3<206:AID-EM8>3.0.CO;2-J.
  • Tolentino, F., P. A. Araujo, E. S. Marques, M. Petreanu, S. F. Andrade, R. Niero, F. F. Perazzo, P. C. P. Rosa, and E. L. Maistro. 2015. In vivo evaluation of the genetic toxicity of Rubus niveus Thunb. (Rosaceae) extract and initial screening of its potential chemoprevention against doxorubicin-induced DNA damage. J. Etnopharmacol 164:89–95. doi:10.1016/j.jep.2015.02.013.
  • Tonin, T. D., L. C. Thiesen, M. L. de Oliveira Nunes, M. F. Broering, M. P. Donato, M. J. Goss, M. Petreanu, R. Niero, I. D. Machado, and J. R. Santin. 2016. Rubus imperialis (Rosaceae) extract and pure compound niga-ichigoside F1: Wound healing and anti-inflammatory effects. Naunyn-Schmiedeberg’s Arch. Pharmacol 389 (11):1235–44. doi:10.1007/s00210-016-1285-8.
  • Valentin-Severin, I., L. L. Hegarat, J. C. Lhuguenot, A. M. Le-Bon, and M. C. Chagnon. 2003. Use of HepG2 cell line for direct or indirect mutagens screening: Comparative investigation between comet and micronucleus assays. Mutat. Res 536 (1–2):79–90. doi:10.1016/S1383-5718(03)00031-7.
  • Viana, A. R., B. G. Noro, D. Santos, K. Wolf, Y. S. Neves, R. N. Moresco, A. F. Ourique, E. M. M. Flores, C. R. B. Rhoden, L. M. F. Krause, et al. 2023. Detection of new phytochemical compounds from Vassobia breviflora (Sendtn.) Hunz: antioxidant, cytotoxic, and antibacterial activity of the hexane extract. J. Toxicol. Environ. Health Part A 86 (2–3):51–68. doi:10.1080/15287394.2022.2156956.
  • Yin, Y., Y. Huang, W. Yang, J. Yuan, M. Xie, Y. Miao, J. Yu, J. Wang, X. Zhang, and B. Wang. 2023. A novel flavonoid and other constituents from Rubus rosifolius S.Vidal (Rosaceae). Nat. Prod. Res. 2023:1–9. doi:10.1080/14786419.2023.2173189.
  • Zor, M., and E. L. Aslan. 2020. Assessment of In Vitro Antigenotoxic Effect of Nigella sativa Oil. Turk J. Pharmaceut. Sci 17 (1):115–18. doi:10.4274/tjps.galenos.2020.09471.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.