Impact of Different Cell Isolation Techniques on Lymphocyte Viability and Function

References

• Curry , M. P. , Norris , S. , Golden‐Mason , L. , Doherty , D. G. , Deignan , T. , Collins , C. , Traynor , O. , McEntee , G. P. , Hegarty , J. E. and O'Farrelly , C. 2000 . Isolation of lymphocytes from normal adult human liver suitable for phenotypic and functional characterization . J. Immunol. Meth. , 242 ( 1‐2 ) : 21 – 31 .
   PubMed Web of Science ®Google Scholar
• Monfalcone , A. P. , Szakal , A. K. and Tew , J. G. 1986 . Increased leukocyte diversity and responsiveness to B‐cell and T‐cell mitogens in cell suspensions prepared by enzymatically dissociating murine lymph nodes . J. Leukoc. Biol. , 39 ( 9 ) : 617 – 628 .
   PubMed Web of Science ®Google Scholar
• Kravchenko , L. , Petrenko , A. , Shanina , I. and Fuller , B. 2002 . A simple non‐enzymatic method for the isolation of high yields of functional rat hepatocytes . Cell Biol. Int. , 26 ( 11 ) : 1003 – 1006 .
   PubMed Web of Science ®Google Scholar
• Klaunig , J. E. , Goldblatt , P. J. , Hinton , D. E. , Lipsky , M. M. , Chacko , J. and Trump , B. F. 1981 . Mouse liver cell culture. I. Hepatocyte Isolation . In. Vitro. , 17 ( 10 ) : 913 – 925 .
   PubMedGoogle Scholar
• Spalding , D. M. , Koopman , W. J. , Eldridge , J. H. , McGhee , J. R. and Steinman , R. M. 1983 . Accessory cells in murine Peyer's patch. I. Identification and enrichment of a functional dendritic cell . J. Exp. Med. , 157 ( 5 ) : 1646 – 1659 .
   PubMed Web of Science ®Google Scholar
• Ford , A. L. , Foulcher , E. , Goodsall , A. L. and Sedgwick , J. D. 1996 . Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell‐surface antigen expression . J. Immunol. Meth. , 194 ( 1 ) : 71 – 75 .
   PubMed Web of Science ®Google Scholar
• Fliegert , F. G. , Tschernig , T. and Pabst , R. 1996 . Comparison of lymphocyte subsets, monocytes, and NK cells in three different lung compartments and peripheral blood in the rats . Exp. Lung Res. , 22 ( 6 ) : 677 – 690 .
   PubMed Web of Science ®Google Scholar
• Lenardo , M. J. 1997 . Introduction: The molecular regulation of lymphocyte apoptosis . Seminars Immunol. , 9 : 1 – 5 .
   PubMedGoogle Scholar
• Gogal , R. M. Jr. , Prater , M. R. , Smith , B. J. , Johnson , M. S. and Holladay , S. D. 2001 . Bilateral dissected spleens and thymuses in rodents exhibit homogeneity in leukocyte markers . Toxicology , 157 ( 3 ) : 217 – 223 .
   PubMed Web of Science ®Google Scholar
• Pai , K. and Sundar , S. 2001 . A simple and inexpensive “cell dissociation sieve‐tissue grinder” apparatus . Indian J. Exp. Biol. , 39 ( 5 ) : 483 – 484 .
   PubMedGoogle Scholar
• Molano , I. D. , Redmond , S. , Sekine , H. , Zhang , X. K. , Reilly , C. , Florence , H. , Ruiz , P. and Gilkeson , G. S. 2003 . Effect of genetic deficiency of terminal deoxynucleotidyl transferase on autoantibody production and renal disease in MRL/lpr mice . Clin. Immunol. , 107 ( 3 ) : 186 – 197 .
   PubMed Web of Science ®Google Scholar
• Dhar , N. , Rao , V. and Tyagi , A. K. 2003 . Skewing the Th1/Th2 responses in mice due to variation in the level of expression of an antigen in a recombinant BCG system . Immunol. Lett. , 88 ( 3 ) : 175 – 184 .
   PubMed Web of Science ®Google Scholar
• Do , Y. , Ryu , S. , Nagarkatti , M. and Nagarkatti , P. S. 2002 . Role of death receptor pathway in estradiol‐induced T‐cell apoptosis in vivo . Toxicol. Sci. , 70 ( 1 ) : 63 – 72 .
   PubMed Web of Science ®Google Scholar
• Walsh , T. J. , McEntee , C. and Dixon , D. M. 1987 . Tissue homogenization with sterile reinforced polyethylene bags for quantitative culture of Candida albicans . J. Clin. Microbiol. , 25 ( 5 ) : 931 – 932 .
   PubMed Web of Science ®Google Scholar
• McHugh , S. , Yamamoto , Y. , Klein , T. W. and Friedman , H. 2000 . Differential expression of IL‐1 and TNF receptors in murine macrophages infected with virulent vs. avirulent Legionella pneumophila . Can. J. Microbiol. , 46 ( 10 ) : 885 – 891 .
   PubMed Web of Science ®Google Scholar
• Witonsky , S. G. , Gogal , R. M. Jr. , Duncan , R. B. and Lindsay , D. S. 2003 . Protective immune response to experimental infection with Sarcocystis neurona in C57BL/6 mice . J. Parasitol , 89 ( 5 ) : 924 – 931 .
   PubMed Web of Science ®Google Scholar
• Zahorsky‐Reeves , J. L. and Wilkinson , J. E. 2001 . The murine mutation scurfy (sf) results in an antigen‐dependent lymphoproliferative disease with altered T cell sensitivity . Eur. J. Immunol. , 31 ( 1 ) : 196 – 204 .
   PubMed Web of Science ®Google Scholar
• Zahorsky‐Reeves , J. L. and Wilkinson , J. E. 2002 . A transgenic mouse strain with antigen‐specific T cells (RAG1K0/sf/OVA) demonstrates that the scurfy (sf) mutation causes a defect in t‐cell tolerization . Comp. Med. , 52 ( 1 ) : 58 – 62 .
   PubMed Web of Science ®Google Scholar
• Donner , K. J. , Becker , K. M. , Hissong , B. D. and Ahmed , S. A. 1999 . Comparison of multiple assays for kinetic detection of apoptosis in thymocytes exposed to dexamethasone or diethylstilbesterol . Cytometry , 35 ( 1 ) : 80 – 90 .
   PubMedGoogle Scholar
• Bidinger , B. , Roxabella , T. , Rossetti , R. G. , Brown , L. , Beltre , R. , Burstein , S. , Lian , J. , Stein , G. S. and Zurier , R. B. 2003 . Ajulemic acid, a nonpsychoactive cannabinoid acid, induces apoptosis in human T lymphocytes . Clin. Immunol. , 108 ( 2 ) : 95 – 102 .
   PubMed Web of Science ®Google Scholar
• Calemine , J. B. , Gogal , R. M. Jr. , Lengi , A. , Sponenberg , S. and Ahmed , A. 2002 . Immunomodulation by diethylstilbestrol is dose and gender related: effects on thymocyte apoptosis and mitogen‐induced proliferation . Toxicology , 178 ( 2 ) : 101 – 118 .
   PubMed Web of Science ®Google Scholar
• Schmid , I. , Uittenbogaart , C. H. , Keld , B. and Giorgi , J. V. 1994 . A rapid method for measuring apoptosis and dual‐color immunofluorescence by single laser flow cytometry . J. Immunol. Meth. , 170 ( 2 ) : 145 – 157 .
   PubMed Web of Science ®Google Scholar
• Christoffers , A. B. , Kreisler , M. and Willershausen , B. 2003 . Effects of estradiol and progesterone on the proliferation of human gingival fibroblasts . Eur. J. Med. Res. , 8 ( 12 ) : 535 – 542 .
   PubMed Web of Science ®Google Scholar
• Ahmed , S. A. , Gogal , R. M. Jr. and Walsh , J. E. 1994 . A new rapid and simple non‐radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to 3H‐thymidine incorporation assay . J. Immunol. Meth. , 170 ( 2 ) : 211 – 224 .
   PubMed Web of Science ®Google Scholar
• Gogal , R. M. Jr. , Ahmed , S. A. , Holladay , S. D. , Walsh , J. E. , Galaburda , A. M. and Rosen , G. D. 2000 . Induced minor malformations in the neocortex of normal mice do not alter immunological functions . Immunol. Invest. , 29 ( 3 ) : 299 – 318 .
   PubMed Web of Science ®Google Scholar
• Altman , S. A. , Randers , L. and Rao , G. 1993 . Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations . Biotechnol. Prog. , 9 ( 6 ) : 671 – 674 .
   PubMed Web of Science ®Google Scholar
• Herbert , W. J. , Wilkinson , P. C. and Stott , D. I. 1995 . The Dictionary of Immunology , 4th Edition 46 New York : Harcourt Brace and Company .
   Google Scholar
• Sekharudu , Y. C. and Rao , V. S. 1984 . The modes of binding methyl‐alpha (and beta)‐D‐glucopyranosides and some of their derivatives to concanavalin A‐‐a theoretical approach . J. Biomol. Struct. Dyn. , 2 ( 1 ) : 41 – 54 .
   PubMed Web of Science ®Google Scholar