Flow‐Cytometric Identification, Enumeration, Purification, and Expansion of CD133⁺ and VEGF‐R2⁺ Endothelial Progenitor Cells from Peripheral Blood

References

• Hristov , M. , Erl , W. and Weber , P. C. 2003 . Endothelial progenitor cells: mobilization, differentiation, and homing . Arterioscler. Thromb. Vasc. Biol. , 23 : 1185 – 1189 .
   PubMed Web of Science ®Google Scholar
• Gratama , J. W. , Menéndez , P. , Kraan , J. and Orfao , A. 2000 . Loss of CD34(+) hematopoietic progenitor cells due to washing can be reduced by the use of fixative‐free erythrocyte lysing reagents . J. Immunol. Meth. , 239 ( 1–2 ) : 13 – 23 .
   Google Scholar
• Cassens , U. , Gutensohn , K. , Garritsen , H. , Kelsch , R. , Kuehnl , P. and Sibrowski , W. 1998 . The influence of different erythrocyte lysing procedures on flow cytometric determination of CD34+ cells in umbilical cord blood transplants . Transfus. Med. , 8 ( 2 ) : 111 – 118 .
   Google Scholar
• Greve , B. , Beller , C. , Cassens , U. , Sibrowski , W. and Göhde , W. 2005 . The impact of erythrocyte lysing procedures on the recovery of hematopoietic progenitor cells in flow cytometric analysis . Stem Cells , (published on line)
   Google Scholar
• Alvarez‐Larran , A. , Jover , L. , Marin , P. and Petriz , J. 2002 . A multicolor, no‐lyse no‐wash assay for the absolute counting of CD34+ cells by flow cytometry . Cytometry , 50 ( 5 ) : 249 – 253 .
   Google Scholar
• Lin , Y. , Weisdorf , D. J. , Solovey , A. and Hebbel , R. P. 2000 . Origins of circulating endothelial cells and endothelial outgrowth from blood . J. Clin. Invest. , 105 : 71 – 77 .
   PubMed Web of Science ®Google Scholar
• Peichev , M. , Naiyer , A. J. , Pereira , D. , Zhu , Z. , Lane , W. J. , Williams , M. , Oz , M. C. , Hicklin , D. J. , Witte , L. , Moore , M. A. and Rafii , S. 2000 . Expression of VEGFR‐2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors . Blood , 95 : 952 – 958 .
   PubMed Web of Science ®Google Scholar
• Quirici , N. , Soligo , D. , Caneva , L. , Servida , F. , Bossolasco , P. and Deliliers , G. L. 2001 . Differentiation and expansion of endothelial cells from human bone marrow CD133+ cells . Brit. J. Haematol. , 115 : 186 – 194 .
   PubMed Web of Science ®Google Scholar
• Kaushal , S. , Amiel , G. E. , Guleserian , K. J. , Shapira , O. M. , Perry , T. , Sutherland , F. W. , Rabkin , E. , Moran , A. M. , Schoen , F. J. , Atala , A. , Soker , S. , Bischoff , J. and Mayer , J. E. Jr. 2001 . Functional small‐diameter neovessels created using endothelial progenitor cells expanded ex vivo . Natl. Med. , 7 : 1035 – 1040 .
   PubMed Web of Science ®Google Scholar
• Asahara , T. , Murohara , T. , Sullivan , A. , Silver , M. , van der Zee , R. , Li , T. , Witzenbichler , B. , Schatteman , G. and Isner , J. M. 1997 . Isolation of putative progenitor endothelial cells for angiogenesis . Science , 275 : 964 – 967 .
   PubMed Web of Science ®Google Scholar
• Cassens , U. , Greve , B. , Tapernon , K. , Nave , B. , Severin , E. , Sibrowski , W. and Göhde , W. 2002 . Anovel true volumetric method for the determination of residual leucocytes in blood components . Vox Sanguinis , 82 : 198 – 206 .
   PubMed Web of Science ®Google Scholar
• Gehling , U. M. , Ergun , S. , Schumacher , U. , Wagener , C. , Pantel , K. , Otte , M. , Schuch , G. , Schafhausen , P. , Mende , T. , Kilic , N. , Kluge , K. , Schafer , B. , Hossfeld , D. K. and Fiedler , W. 2000 . In vitro differentiation of endothelial cells from AC133‐positive progenitor cells . Blood , 95 : 3106 – 3112 .
   PubMed Web of Science ®Google Scholar
• Handgretinger , R. , Gordon , P. R. , Leimig , T. , Chen , X. , Buhring , H. J. , Niethammer , D. and Kuci , S. 2003 . Biology and plasticity of CD133+ hematopoietic stem cells . Ann. N.Y. Acad. Sci. , 996 : 141 – 151 .
   PubMed Web of Science ®Google Scholar
• Greve , B. , Beller , C. , Cassens , U. , Sibrowski , W. , Severin , E. and Göhde , W. 2003 . High‐Grade Loss of Leukocytes and Hematopoietic Progenitor Cells Caused by Erythrocyte‐Lysing Procedures for Flow Cytometric Analyses . J. Hematother. Stem Cell Res. , : 321 – 330 .
   Google Scholar
• Vuorte , J. , Jansson , S. E. and Repo , H. 2001 . Evaluation of red blood cell lysing solutions in the study of neutrophil oxidative burst by the DCFH assay . Cytometry , 43 ( 4 ) : 290 – 296 .
   Google Scholar
• Bossuyt , X. , Marti , G. E. and Fleisher , T. A. 1997 . Comparative analysis of whole blood lysis methods for flow cytometry . Cytometry , 30 ( 3 ) : 124 – 133 .
   PubMedGoogle Scholar
• Rustemeyer , P. , Wittkowski , W. , Jurk , K. and Koller , A. 2006 . Optimized flow cytometric analysis of endothelial progenitor cells in peripheral blood . J. Immunoassay Immunochem. , 27 ( 1 ) : 77 – 88 .
   PubMed Web of Science ®Google Scholar