95
Views
1
CrossRef citations to date
0
Altmetric
Original Articles

A modified sandwich ELISA for accurate measurement of HbF in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1

, , , &

References

  • Weatherall, D. J.; Clegg, J. B. The Thalassaemia Syndromes, 4th ed.; Blackwell Scientific: Oxford, 2001.
  • Boyer, S. H.; Belding, T. K.; Margolet, L.; Noyes, A. N. Fetal Hemoglobin Restriction to a Few Erythrocytes (F Cells) in Normal Human Adults. Science 1975, 188, 4186, 361–363.
  • Wood, W. G.; Increased HbF in Adult Life. In Bailliere’s Clinical Haematology: International Practice and Research; Higgs, D. R., Weatherall, D. J., Eds.; Bailliere Tindall, W.B. Saunders: London, 1993. pp 177–213.
  • Charache, S.;. Fetal Hemoglobin, Sickling, and Sickle Cell Disease. Adv. Pediatr. 1990, 37, 1–31.
  • Winichagoon, P.; Thonglairoam, V.; Fucharoen, S.; Wilairat, P.; Fukumaki, Y.; Wasi, P. Severity Differences in β-thalassaemia/haemoglobin E Syndromes: Implication of Genetic Factors. Br. J. Haematol. 1993, 83, 4, 633–639.
  • Chang, Y. P.; Littera, R.; Garau, R.; Smith, K. D.; Dover, G. J.; Iannelli, S.; Cacace, E.; Contu, L. The Role of Heterocellular Hereditary Persistence of Fetal Haemoglobin in βo-thalassaemia Intermedia. Br. J. Haematol. 2001, 114, 4, 899–906.
  • Betke, K.; Marti, H. R.; Schlicht, I. Estimation of Small Percentages of Foetal Haemoglobin. Nature 1959, 184, 1877–1878.
  • Wood, WG. Hemoglobin Analysis. In The Thalassemia : Methods in Hematology; Weatherall DJ, ed.; Churchill Livingstone: New York, 1983, pp 31–53.
  • Armstrong, D. H.; Schroeder, W. A.; Fenninger, W. D. A Comparison of the Percentage of Fetal Hemoglobin in Human Umbilical Cord Blood as Determined by Chromatography and by Alkali Denaturation. Blood 1963, 22, 554–565.
  • Ou, C. N.; Rognerud, C. L. Rapid Analysis of Hemoglobin Variants by Cation-Exchange HPLC. Clin. Chem. 1993, 39, 5, 820–824.
  • Fucharoen, S.; Winichagoon, P.; Wisedpanichkit, R.; Sae-Ngow, B.; Sriphanich, R.; Oncoung, W.; Muangsapaya, W.; Chowthaworn, J.; Kanokpongsakdi, S.; Bunyaratvej, A.; et al. Prenatal and Postnatal Diagnoses of Thalassemias and Hemoglobinopathies by HPLC. Clin. Chem. 1998, 44(4), 740–748.
  • Ou, C. N.; Rognerud, C. L. Diagnosis of Hemoglobinopathies: Electrophoresis vs. HPLC. Clin. Chim. Acta 2001, 313, 1–2, 187–194.
  • Wang, J.; Zhou, S.; Huang, W.; Liu, Y.; Cheng, C.; Lu, X.; Cheng, J. CE-based Analysis of Hemoglobin and Its Applications in Clinical Analysis. Electrophoresis 2006, 27(15), 3108–3124. DOI: 10.1002/elps.200500833.
  • Winichagoon, P.; Svasti, S.; Munkongdee, T.; Chaiya, W.; Boonmongkol, P.; Chantrakul, N.; Fucharoen, S. Rapid Diagnosis of Thalassemias and Other Hemoglobinopathies by Capillary Electrophoresis System. Transl. Res. 2008, 152(4), 178–184. DOI: 10.1016/j.trsl.2008.08.004.
  • Sangkitporn, S.; Sangkitporn, S. K.; Tanjatham, S.; Suwannakan, B.; Rithapirom, S.; Yodtup, C.; Yowang, A.; Duangruang, S. Multicenter Validation of Fully Automated Capillary Electrophoresis Method for Diagnosis of Thalassemias and Hemoglobinopathies in Thailand. Southeast Asian J. Trop. Med. Public Health 2011, 42, 5, 1224–1232.
  • Moscoso, H.; Shyamala, M.; Kiefer, C. R.; Garver, F. A. Monoclonal Antibody to the Gamma Chain of Human Fetal Hemoglobin Used to Develop an Enzyme Immunoassay. Clin. Chem. 1989, 35, 10, 2066–2069.
  • Makler, M. T.; Pesce, A. J. ELISA Assay for Measurement of Human Hemoglobin A and Hemoglobin F. Am. J. Clin. Pathol. 1980, 74, 5, 673–676.
  • Pata, S.; Pongpaiboon, M.; Surinkaew, S.; Laopajon, W.; Kasinrerk, W. Large-Scale Preparation of Purified Monoclonal Antibody from Cell Culture Supernatant: A Case Study with a Monoclonal Antibody to α-globin Chain. J. Assoc. Med. Sci. 2018, 51, 2, 85–92.
  • Kuntaruk, S.; Tatu, T.; Keowkarnkah, T.; Kasinrerk, W. Sandwich ELISA for Hemoglobin A2 Quantification and Identification of β-thalassemia Carriers. Int. J. Hematol. 2010, 91(2), 219–228. DOI: 10.1007/s12185-009-0490-3.
  • Pakdeepak, K.; Kerdpoo, S.; Tatu, T. Fast Preparative Separation of Human Hemoglobins A, A2, E, F, Bart’s and Portland I. Bull. Chiang Mai. Assoc. Med. Sci. 2015, 48, 2, 100–106.
  • Tayapiwatana, C.; Kuntaruk, S.; Tatu, T.; Chiampanichayakul, S.; Munkongdee, T.; Winichagoon, P.; Fuchareon, S.; Kasinrerk, W. Simple Method for Screening of α-thalassaemia 1 Carriers. Int. J. Hematol. 2009, 89(5), 559–567. DOI: 10.1007/s12185-009-0331-4.
  • Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals, 8th ed.; The National Academies Press: Washington DC, 2011.
  • Feteanu, A.; Labeled Antibodies in Biology and Medicine; McGraw-Hill International Book Company: New York, 1978
  • Srisuwan, W.; Tatu, T. Diagnosis of Thalassemia Carriers Commonly Found in Northern Thailand via a Combination of MCV or MCH and PCR-based Method. Bull. Chiang Mai. Assoc. Med. Sci. 2013, 46, 1, 22–32.
  • Tatu, T.; Kiewkarnkha, T.; Khuntarak, S.; Khamrin, S.; Suwannasin, S.; Kasinrerk, W. Screening for Co-Existence of Alpha-Thalassemia in Beta-Thalassemia and in HbE Heterozygotes via an Enzyme-Linked Immunosorbent Assay for Hb Bart’s and Embryonic Zeta-Globin Chain. Int. J. Hematol. 2012, 95(4), 386–393. DOI: 10.1007/s12185-012-1039-4.
  • Thein, S. L.; Genetic Modifiers of β-thalassemia. Haematologica 2005, 90, 5, 649–660.
  • Harmer, I. J.; Samuel, D. The FITC-anti-FITC System Is a Sensitive Alternative to Biotin-Streptavidin in ELISA. J. Immunol. Methods 1989, 122, 1, 115–121.
  • Higgs, D. R.; Weatherall, D. J. The Alpha Thalassaemias. Cell. Mol. Life Sci. 2009.66, 7, 1154–1162.
  • Chung, S. W.; Wong, S. C.; Clarke, B. J.; Patterson, M.; Walker, W. H.; Chui, D. H. Human Embryonic ζ-globin Chains in Adult Patients with α-thalassemias. Proc. Natl. Acad. Sci. USA 1984, 81, 19, 6188–6191.
  • Fucharoen, S.; Winichagoon, P.; Siritanaratkul, N.; Chowthaworn, J.; Pootrakul, P. Alpha- and Beta-Thalassemia in Thailand. Ann. N. Y. Acad. Sci. 1998, 850, 412–414.
  • Lewis, S.; Bain, B.; Bates, L. Dacie and Lewis Practical Haematology, 10th ed.; Churchill Livingstone Elsevier: Hong Kong, 2009.
  • Rochette, J.; Craig, J. E.; Thein, S. L. Fetal Hemoglobin Levels in Adults. Blood Rev. 1994, 8, 4, 213–224.
  • Tan, G. B.; Aw, T. C.; Dunstan, R. A.; Lee, S. H. Evaluation of High Performance Liquid Chromatography for Routine Estimation of Haemoglobins A2 and F. J. Clin. Pathol. 1993, 46, 9, 852–856.
  • Jean, R.; Christian, G.; Didier, H.; Mireille, M.; Catherine, B.; Josiane, B.-M.; Claude, P.; Henri, W.; Frederic, G. Cation-Exchange HPLC Evaluated for Presumptive Identification of Hemoglobin Variants. Clin. Chem. 1997, 43, 1, 34–39.
  • The Working Party of the General Haematology Task Force of the British Committee for Standards in Haematology. The Laboratory Diagnosis of Haemoglobinopathies. Br. J. Haematol. 1998, 101(4), 783–792.
  • Clarke, G. M.; Higgins, T. N. Laboratory Investigation of Hemoglobinopathies and Thalassemias: Review and Update. Clin. Chem. 2000, 46, 8 Pt 2, 1284–1290.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.