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Research Article

Direct immunofluorescence on formalin-fixed, paraffin-embedded versus fresh frozen human renal biopsies: a comparative study

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References

  • Mohammadzadeh, S.; Aghakhaninejad, F.; Azad, F.; Derakhshan, D.; Soleimani, N. Diagnostic Accuracy of Direct Immunofluorescence Test on Paraffin-Embedded Blocks in Comparison with Frozen Section Blocks in Renal Biopsies. Int. J. Nephrol. 2022, 2022, 4974031. DOI: 10.1155/2022/4974031.
  • Wagrowska-Danilewicz, M.; Danilewicz, M. Immunofluorescence on Paraffin-Embedded Sections in Evaluation of Immune Complex Deposits in Renal Biopsy Specimens. Pol. J .Pathol. 2009, 60(1), 3–9.
  • Alwahaibi, N. Y.; Alsidiri, R. M.; Alsinawi, T. A.; Almalki, W. H.; Alsinawi, S. S.; Alriyami, M. A.; Immunoperoxidase; On Formalin-Fixed, I. Paraffin-Embedded Tissue Sections versus Immunofluorescence on Frozen Sections in the Assessment of Renal Biopsies. Indian J. Nephrol. 2020, 30(1), 8–13. DOI: 10.4103/ijn.IJN_356_18.
  • Tang, X.; He, J.; Partin, J.; Vafai, A. Comparative analysis of direct fluorescence, zenon labeling, and quantum dot nanocrystal technology in immunofluorescence staining. J. Immunoassay Immunochem. 2010, 31(3), 250–257. DOI: 10.1080/10739149.2010.488620.
  • AL-Roubaey, D. The Correlation Between Anti-Neutrophil Cytoplasmic Antibody and Two Cytokines (IL-18, TNF-α) in Rapidly Progressive Glomerulonephritis Disease. AL-Taqani. 2013, 26(3), 73–82.
  • AL-Roubaey, D. Clinical Measures for the Cytokine Levels After and Before Hirudotherapy in Rheumatoid Arthritis Patients. J .Global. Pharma .Tech. 2018, 10(3), 578–586.
  • Das, N.; Lakadong, R. O.; Dey, B.; Raphael, V. A Comparative Study of Immunofluorescence on Formalin-Fixed, Paraffin-Embedded versus Fresh Frozen Kidney Biopsy. Cureus. 2023, 15(6), e40978. DOI: 10.7759/cureus.40978.
  • Nada, R.; Kumar, A.; Kumar, V. G.; Gupta, K. L.; Joshi, K. Unmasking of Complements Using Proteinase-K in Formalin Fixed Paraffin Embedded Renal Biopsies. Indian J. Nephrol. 2016, 26(3), 182–187. DOI: 10.4103/0971-4065.159558.
  • Malipatel, R.; Vellaisamy, G.; Rout, P. Immunofluorescence on Formalin Fixed Paraffin Embedded Renal Tissue Sections: A Retrospective Study. J. Clin. Diagnostic. Res. 2022, 16(11), EC05–08. DOI: 10.7860/JCDR/2022/58418.17003.
  • Curran, R. C.; Gregory, J. Demonstration of Immunoglobulin in Cryostat and Paraffin Sections of Human Tonsil by Immunofluorescence and Immunoperoxidase Techniques. Effects of Processing on Immunohistochemical Performance of Tissues and on the Use of Proteolytic Enzymes to Unmask Antigens in Sections. J. Clin. Pathol. 1978, 31(10), 974–983. DOI: 10.1136/jcp.31.10.974.
  • Dorsett, B. H.; Ioachim, H. L. A Method for the Use of Immunofluorescence on Paraffin-Embedded Tissues. Am. J. Clin. Pathol. 1978, 69(1), 66–72. DOI: 10.1093/ajcp/69.1.66.
  • Qualman, S. J.; Keren, D. F. Immunofluorescence of Deparaffinized, Trypsin-Treated Renal Tissues. Preservation of Antigens as an Adjunct to Diagnosis of Disease. Lab. Invest. 1979, 41(6), 483–489.
  • Choi, Y. J.; Reiner, L. Immunofluorescence of Renal Lesions in Paraffin-Embedded and Fresh-Frozen Sections. Am. J. Clin. Pathol. 1980, 73(1), 116–119. DOI: 10.1093/ajcp/73.1.116.
  • Enestrom, S.; Hed, J.; Hultman, P. Detection of Immune Deposits in Glomeruli: A Comparative Study of Paraffin-Embedded, Enzyme-Treated Sections and Cryostat Sections as Substrates in Immunofluorescence. J. Immunol. Methods. 1980, 37(3–4), 343–351. DOI: 10.1016/0022-1759(80)90320-8.
  • Sinclair, R. A.; Burns, J.; Dunnill, M. S. Immunoperoxidase Staining of Formalin-Fixed, Paraffin-Embedded, Human Renal Biopsies with a Comparison of the Peroxidase-Antiperoxidase (PAP) and Indirect Methods. J. Clin. Pathol. 1981, 34(8), 859–865. DOI: 10.1136/jcp.34.8.859.
  • Bolton, W. K.; Mesnard, R. M. New technique of kidney tissue processing for immunofluorescence microscopy: formol sucrose/gum sucrose/paraffin. Lab. Invest. 1982, 47(2), 206–213.
  • Casanova, S.; Donini, U.; Zini, N.; Morelli, R.; Zucchelli, P. Immunohistochemical Staining on Hydroxyethyl-Methacrylate-Embedded Tissues. J. Histochem. Cytochem. 1983, 31(8), 1000–1004. DOI: 10.1177/31.8.6345656.
  • Fogazzi, G. B.; Bajetta, M.; Banfi, G.; Mihatsch, M. Comparison of Immunofluorescent Findings in Kidney After Snap-Freezing and Formalin Fixation. Pathol. Res. Pract. 1989, 185(2), 225–230. DOI: 10.1016/S0344-0338(89)80256-0.
  • Bisceglia, M.; De Luca, N.; Scaramuzzi, F.; Vairo, M.; Zaffarano, L.; D’Errico, M. Techniques for the Diagnosis of Kidney Diseases. Is There a Role for the Immunoperoxidase Method in the Evaluation of Immunologic Glomerulopathy on Biopsy Specimens, Including Those in Paraffin? Our Experience: Preliminary Results. Pathologica. 1995, 87(6), 617–623.
  • Chowdhury, A. R.; Ehara, T.; Higuchi, M.; Hora, K.; Shigematsu, H. Immunohistochemical Detection of Immunoglobulins and Complements in Formaldehyde-Fixed and Paraffin-Embedded Renal Biopsy Tissues; an Adjunct for Diagnosis of Glomerulonephritis. Nephrology (Carlton). 2005, 10(3), 298–304. DOI: 10.1111/j.1440-1797.2005.00396.x.
  • Molne, J.; Breimer, M. E.; Svalander, C. T. Immunoperoxidase versus Immunofluorescence in the Assessment of Human Renal Biopsies. Am. J. Kidney Dis. 2005, 45(4), 674–683. DOI: 10.1053/j.ajkd.2004.12.019.
  • Nasr, S. H.; Galgano, S. J.; Markowitz, G. S.; Stokes, M. B.; D’Agati, V. D. Immunofluorescence on pronase-digested paraffin sections: a valuable salvage technique for renal biopsies. Kidney Int. 2006, 70(12), 2148–2151. DOI: 10.1038/sj.ki.5001990.
  • Karasalihovic, Z.; Iljazovic, E.; Ferluga, D.; Cickusic, E.; Mustedanagic Mujanovic, J.; Stahov, J.; Skaljic, I. Direct immunofluorescence and immunohistochemistry in diagnostics of glomerulonephritis. Bosn. J. Basic Med. Sci. 2008, 8(1), 12–19. DOI: 10.17305/bjbms.2008.2989.
  • Shubham, S.; Bhardwaj, M.; Mahapatra, H. S. Comparative evaluation of immunoperoxidase versus immunofluorescent techniques in interpretation of kidney biopsies. Indian J. Pathol. Microbiol. 2016, 59(3), 305–309. DOI: 10.4103/0377-4929.188105.
  • Singh, G.; Singh, L.; Ghosh, R.; Nath, D.; Dinda, A. K. Immunofluorescence on Paraffin Embedded Renal Biopsies: Experience of a Tertiary Care Center with Review of Literature. World. J .Nephrol. 2016, 5(5), 461–470. DOI: 10.5527/wjn.v5.i5.461.
  • Nasr, S. H.; Fidler, M. E.; Said, S. M. Paraffin Immunofluorescence: A Valuable Ancillary Technique in Renal Pathology. Kidney. Int. Rep. 2018, 3(6), 1260–1266. DOI: 10.1016/j.ekir.2018.07.008.
  • Solanki, R.; Solanki, M. K.; Hemrajani, D.; Saha, J. A Study of Detection and Comparison of Immunofluorescence on Formalin-Fixed Paraffin-Embedded Tissue with Fresh Frozen Renal Biopsy Specimen. Saudi. J. Kidney. Dis. Transpl. 2019, 30(2), 350–358. DOI: 10.4103/1319-2442.256842.
  • Churg, J.; Bernstein, J.; Glassock, R. Renal Disease: Classification and Atlas of Glomerular Diseases, 2nd ed.; Igaku-Shoin: New York, Tokyo, 1995.
  • Nambirajan, A.; Bhowmik, D.; Singh, G.; Agarwal, S. K.; Dinda, A. K. Monoclonal Gammopathy of Renal Significance with Light-Chain Deposition Disease Diagnosed Postrenal Transplant: A Diagnostic and Therapeutic Challenge. Transpl. Int. 2015, 28(3), 375–379. DOI: 10.1111/tri.12497.
  • Mubarak, M.; Kazi Javed, I.; Kulsoom, U.; Ishaque, M. Detection of Immunoglobulins and Complement Components in Formalin Fixed and Paraffin Embedded Renal Biopsy Material by Immunoflourescence Technique. J. Nephropathol. 2012, 1(2), 91–100. DOI: 10.5812/nephropathol.7518.
  • Wagrowska-Danilewicz, M.; Zeromski, J. Immunofluorescent evaluation of renal biopsy: current point of view. Pol. J .Pathol. 2010, 61(2), 83–88.
  • Sethi, S.; Haas, M.; Markowitz, G. S.; D’Agati, V. D.; Rennke, H. G.; Jennette, J. C.; Bajema, I. M.; Alpers, C. E.; Chang, A.; Cornell, L. D., et al. Mayo Clinic/Renal Pathology Society Consensus Report on Pathologic Classification, Diagnosis, and Reporting of GN. J. Am. Soc. Nephrol. 2016, 27(5), 1278–1287.
  • Becheva, Z. R.; Gabrovska, K. I.; Godjevargova, T. I. Comparison Between Direct and Indirect Immunofluorescence Method for Determination of Somatic Cell Count. Chem. Pap. 2018, 72(8), 1861–1867. DOI: 10.1007/s11696-018-0445-3.
  • Shi, S.; Cheng, Q.; Zhang, P.; Wang, N.; Zheng, Y.; Bai, X. Y.; Chen, X. Immunofluorescence with Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens. Am. J. Clin. Pathol. 2013, 139(1), 71–78. DOI: 10.1309/AJCPRZG8EXN7BAID.

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