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Toxicology Mechanisms and Methods Volume 30, 2020 - Issue 2
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Original Articles

Hep G2 cell culture confluence measurement in phase-contrast micrographs – a user-friendly, open-source software-based approach

Yordan I. YordanovDepartment of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, Sofia, BulgariaCorrespondence[email protected]
ORCID Iconhttp://orcid.org/0000-0003-1029-9870
ORCID Icon
Pages 146-152 | Received 08 Oct 2019, Accepted 13 Nov 2019, Published online: 28 Nov 2019

References

  • Aftab O, Nazir M, Fryknäs M, Hammerling U, Larsson R, Gustafsson MG. 2014. Label free high throughput screening for apoptosis inducing chemicals using time-lapse microscopy signal processing. Apoptosis. 19(9):1411–1418.
  • Bensch R, Ronneberger O. 2015. Cell segmentation and tracking in phase contrast images using graph cut with asymmetric boundary costs. In: 2015 IEEE 12th International Symposium on Biomedical Imaging (ISBI); Apr 16–19; Brooklyn, NY, USA: IEEE. p. 1220–1223. [accessed 2019 Aug 13]. http://ieeexplore.ieee.org/document/7164093/.
  • Bruggisser R, Daeniken K, von Jundt G, Schaffner W, Tullberg-Reinert H. 2002. Interference of plant extracts, phytoestrogens and antioxidants with the MTT tetrazolium assay. Planta Med. 68(5):445–448.
  • Busschots S, O’Toole S, O’Leary JJ, Stordal B. 2015. Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines. MethodsX. 2:8–13.
  • Čepa M. 2018. Segmentation of total cell area in brightfield microscopy images. Methods Protoc. 1(4):43.
  • Chalfoun J, Majurski M, Peskin A, Breen C, Bajcsy P, Brady M. 2015. Empirical gradient threshold technique for automated segmentation across image modalities and cell lines. J Microsc. 260(1):86–99.
  • Cook B, Proctor D, Bromberg R, LaPointe NE, Feinstein SC, Wilson L. 2017. Digital quantification of neurite outgrowth and retraction by phase-contrast microscopy: a tau perspective. Methods Cell Biol. 141:217–228. [accessed 2019 Aug 13]. https://linkinghub.elsevier.com/retrieve/pii/S0091679X17300742.
  • Cordelières FP, Petit V, Kumasaka M, Debeir O, Letort V, Gallagher SJ, Larue L. 2013. Automated cell tracking and analysis in phase-contrast videos (iTrack4U): development of java software based on combined mean-shift processes. PLoS One. 8(11):e81266.
  • Jones TR, Kang IH, Wheeler DB, Lindquist RA, Papallo A, Sabatini DM, Golland P, Carpenter AE. 2008. CellProfiler analyst: data exploration and analysis software for complex image-based screens. BMC Bioinformatics. 9(1):482.
  • Karakaş D, Ari F, Ulukaya E. 2017. The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. Turk J Biol. 41(6):919–925.
  • Kleijn A, Kloezeman JJ, Balvers RK, van der Kaaij M, Dirven CMF, Leenstra S, Lamfers M. 2016. A systematic comparison identifies an ATP-based viability assay as most suitable read-out for drug screening in glioma stem-like cells. Stem Cells Int. 2016:1. [accessed 2019 Oct 2]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4871979/.
  • Langdon SP. 2004. Cancer cell culture methods and protocols. Totowa (NJ): Humana Press. [accessed 2016 Feb 5]. http://site.ebrary.com/id/10181489.
  • Logan DJ, Shan J, Bhatia SN, Carpenter AE. 2016. Quantifying co-cultured cell phenotypes in high-throughput using pixel-based classification. Methods. 96:6–11.
  • Lojk J, Čibej U, Karlaš D, Šajn L, Pavlin M. 2015. Comparison of two automatic cell-counting solutions for fluorescent microscopic images. J Microsc. 260(1):107–116.
  • McClatchey AI, Yap AS. 2012. Contact inhibition (of proliferation) redux. Curr Opin Cell Biol. 24(5):685–694.
  • Riss TL, Moravec RA, Niles AL, Benink HA, Worzella TJ, Minor L. 2004. Cell viability assays. In: Sittampalam GS, Coussens NP, Nelson H, Arkin M, Auld D, Austin C, Bejcek B, Glicksman M, Inglese J, Iversen PW, editors. Assay guidance manual. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences. [accessed 2016 May 24]. http://www.ncbi.nlm.nih.gov/books/NBK144065/.
  • Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, et al. 2012. Fiji: an open-source platform for biological-image analysis. Nat Methods. 9(7):676–682.
  • Schneider CA, Rasband WS, Eliceiri KW. 2012. NIH image to image J: 25 years of image analysis. Nat Methods. 9(7):671–675.
  • Sommer C, Straehle C, Kothe U, Hamprecht FA. 2011. Ilastik: interactive learning and segmentation toolkit. In: 2011 IEEE International Symposium on Biomedical Imaging: From Nano to Macro; 30 Mar–2 Apr; Chicago, IL, USA: IEEE. p. 230–233. [accessed 2019 Sep 4]. http://ieeexplore.ieee.org/document/5872394/.
  • Vicar T, Balvan J, Jaros J, Jug F, Kolar R, Masarik M, Gumulec J. 2019. Cell segmentation methods for label-free contrast microscopy: review and comprehensive comparison. BMC Bioinformatics. 20(1):360. [accessed 2019 Oct 2]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599268/.

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