Generation of therapeutic immunoconjugates via Residue-Specific Conjugation Technology (RESPECT) utilizing a native cysteine in the light chain framework of Oryctolagus cuniculus

References

• Deonarain MP, Yahioglu G, Stamati I, Marklew J. Emerging formats for next-generation antibody drug conjugates. Expert Opin Drug Discov 2015; 10(5):463-81; PMID:25797303; https://doi.org/10.1517/17460441.2015.1025049
   PubMed Web of Science ®Google Scholar
• de Goeij BE, Lambert JM. New developments for antibody-drug conjugate-based therapeutic approaches. Curr Opin Immunol 2016 Jun; 40:14-23; PMID:26963132; https://doi.org/10.1016/j.coi.2016.02.008
   PubMed Web of Science ®Google Scholar
• Bakhtiar R. Antibody drug conjugates. Biotechnol Lett 2016; 38(10):1655-64; PMID:27334710; https://doi.org/10.1007/s10529-016-2160-x
   PubMed Web of Science ®Google Scholar
• Tsuchikama K, An Z. Antibody-drug conjugates: recent advances in conjugation and linker chemistries. Protein Cell 2016; 14; PMID:27743348; https://doi.org/10.1007/s13238-016-0323-0
   PubMedGoogle Scholar
• Berger GK, McBride A, Lawson S, Royball K, Yun S, Gee K, Bin Riaz I, Saleh AA, Puvvada S, Anwer F. Brentuximab vedotin for treatment of non-Hodgkin lymphomas: A systematic review. Crit Rev Oncol Hematol 2017; 109:42-50; PMID:28010897; https://doi.org/10.1016/j.critrevonc.2016.11.009
   PubMed Web of Science ®Google Scholar
• Kline T, Steiner AR, Penta K, Sato AK, Hallam TJ, Yin G. Methods to Make Homogenous Antibody Drug Conjugates. Pharm Res 2015; 32(11):3480-93; PMID:25511917; https://doi.org/10.1007/s11095-014-1596-8
   PubMed Web of Science ®Google Scholar
• Sochaj AM, Świderska KW, Otlewski J. Current methods for the synthesis of homogeneous antibody-drug conjugates. Biotechnol Adv 2015; 33(6 Pt 1):775-84; PMID:28178520; https://doi.org/10.1016/j.biotechadv.2015.05.001
   PubMed Web of Science ®Google Scholar
• Zimmerman ES, Heibeck TH, Gill A, Li X, Murray CJ, Madlansacay MR, Tran C, Uter NT, Yin G, Rivers PJ, et al. Production of site-specific antibody-drug conjugates using optimized non-natural amino acids in a cell-free expression system. Bioconjug Chem 2014; 25(2):351-61; PMID:24437342; https://doi.org/10.1021/bc400490z
   PubMed Web of Science ®Google Scholar
• Jeger S, Zimmermann K, Blanc A, Grünberg J, Honer M, Hunziker P, Struthers H, Schibli R. Site-specific and stoichiometric modification of antibodies by bacterial transglutaminase. Angew Chem Int Ed Engl 2010; 49(51):9995-7; PMID:21110357; https://doi.org/10.1002/anie.201004243
   PubMed Web of Science ®Google Scholar
• Strop P, Liu SH, Dorywalska M, Delaria K, Dushin RG, Tran TT, Ho WH, Farias S, Casas MG, Abdiche Y, et al. Location matters: site of conjugation modulates stability and pharmacokinetics of antibody drug conjugates. Chem Biol 2013; 20(2):161-7; PMID:23438745; https://doi.org/10.1016/j.chembiol.2013.01.010
   PubMed Web of Science ®Google Scholar
• Junutula JR, Raab H, Clark S, Bhakta S, Leipold DD, Weir S, Chen Y, Simpson M, Tsai SP, Dennis MS, et al. Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index. Nat Biotechnol 2008; 26(8):925-32; PMID:18641636; https://doi.org/10.1038/nbt.1480
   PubMed Web of Science ®Google Scholar
• Strosberg AD, Margolies MN, Haber E. The interdomain disulfide bond of a homogeneous rabbit pneumococcal antibody light chain. J Immunol 1975; 115(5):1422-4; PMID:240892
   PubMed Web of Science ®Google Scholar
• Stammers TA, Coulombe R, Rancourt J, Thavonekham B, Fazal G, Goulet S, Jakalian A, Wernic D, Tsantrizos Y, Poupart MA, et al. Discovery of a novel series of non-nucleoside thumb pocket 2 HCV NS5B polymerase inhibitors. Bioorg Med Chem Lett 2013; 23(9):2585-9; PMID:23545108; https://doi.org/10.1016/j.bmcl.2013.02.110
   PubMed Web of Science ®Google Scholar
• Ma J, Tang WK, Esser L, Pastan I, Xia D. Recognition of mesothelin by the therapeutic antibody MORAb-009: structural and mechanistic insights. J Biol Chem 2012; 287(40):33123-31; PMID:22787150; https://doi.org/10.1074/jbc.M112.381756
   PubMed Web of Science ®Google Scholar
• Lammens A, Baehner M, Kohnert U, Niewoehner J, von Proff L, Schraeml M, Lammens K, Hopfner KP. Crystal Structure of Human TWEAK in Complex with the Fab Fragment of a Neutralizing Antibody Reveals Insights into Receptor Binding. PLoS One 2013; 8(5):e62697; PMID:23667509; https://doi.org/10.1371/journal.pone.0062697
   PubMed Web of Science ®Google Scholar
• Lee P, Wu X. Review: modifications of human serum albumin and their binding effect. Curr Pharm Des 2015; 21(14):1862-5; PMID:25732553; https://doi.org/10.2174/1381612821666150302115025
   PubMed Web of Science ®Google Scholar
• Buchanan A, Clementel V, Woods R, Harn N, Bowen MA, Mo W, Popovic B, Bishop SM, Dall'Acqua W, Minter R, et al. Engineering a therapeutic IgG molecule to address cysteinylation, aggregation and enhance thermal stability and expression. MAbs 2013; 5(2):255-62; PMID:23412563; https://doi.org/10.4161/mabs.23392
   PubMed Web of Science ®Google Scholar
• Junutula JR, Bhakta S, Raab H, Ervin KE, Eigenbrot C, Vandlen R, Scheller RH, Lowman HB. Rapid identification of reactive cysteine residues for site-specific labeling of antibody-Fabs. J Immunol Methods 2008; 332(1–2):41-52; PMID:18230399; https://doi.org/10.1016/j.jim.2007.12.011
   PubMed Web of Science ®Google Scholar
• Zheng M, Zheng L, Zhang P, Li J, Zhang Y. Development of bioorthogonal reactions and their applications in bioconjugation. Molecules 2015; 20(2):3190-205; PMID:25690284; https://doi.org/10.3390/molecules20023190
   PubMed Web of Science ®Google Scholar
• Zhou Q, Gui J, Pan CM, Albone E, Cheng X, Suh EM, Grasso L, Ishihara Y. Baran PS Bioconjugation by native chemical tagging of C-H bonds. J Am Chem Soc 2013; 135(35):12994-7; PMID:23957305; https://doi.org/10.1021/ja407739y
   PubMed Web of Science ®Google Scholar
• Ivanov S, Liao SY, Ivanova A, Danilkovitch-Miagkova A, Tarasova N, Weirich G, Merrill MJ, Proescholdt MA, Oldfield EH, Lee J, et al. Expression of hypoxia-inducible cell-surface transmembrane carbonic anhydrases in human cancer. Am J Pathol 2001; 158(3):905-19; PMID:11238039; https://doi.org/10.1016/S0002-9440(10)64038-2
   PubMed Web of Science ®Google Scholar
• Carlin S, Khan N, Ku T, Longo VA, Larson SM, Smith-Jones PM. Molecular targeting of carbonic anhydrase IX in mice with hypoxic HT29 colorectal tumor xenografts. PLoS One 2010; 5(5):e10857; PMID:20523727; https://doi.org/10.1371/journal.pone.0010857
   PubMed Web of Science ®Google Scholar
• Gubbels JA, Belisle J, Onda M, Rancourt C, Migneault M, Ho M, Bera TK, Connor J, Sathyanarayana BK, Lee B, et al. Mesothelin-MUC16 binding is a high affinity, N-glycan dependent interaction that facilitates peritoneal metastasis of ovarian tumors. Mol Cancer 2006; 5(1):50; PMID:17067392; https://doi.org/10.1186/1476-4598-5-50
   PubMed Web of Science ®Google Scholar
• Ordóñez NG. Application of mesothelin immunostaining in tumor diagnosis. Am J Surg Pathol 2003; 27(11):1418-28; PMID:12883236; https://doi.org/10.1097/00000478-200311000-00003
   PubMed Web of Science ®Google Scholar
• Hassan R, Kindler HL, Jahan T, Bazhenova L, Reck M, Thomas A, Pastan I, Parno J, O'Shannessy DJ, Fatato P, et al. Phase II clinical trial of amatuximab, a chimeric antimesothelin antibody with pemetrexed and cisplatin in advanced unresectable pleural mesothelioma. Clin Cancer Res 2014; 20(23):5927-36; PMID:25231400; https://doi.org/10.1158/1078-0432.CCR-14-0804
   PubMed Web of Science ®Google Scholar
• Lindenberg L, Thomas A, Adler S, Mena E, Kurdziel K, Maltzman J, Wallin B, Hoffman K, Pastan I, Paik CH, et al. Safety and biodistribution of 111In-amatuximab in patients with mesothelin expressing cancers using Single Photon Emission Computed Tomography-Computed Tomography (SPECT-CT) imaging. Oncotarget 2015; 6(6):4496-504; PMID:25756664; https://doi.org/10.18632/oncotarget.2883
   PubMed Web of Science ®Google Scholar
• Hassan R, Thomas A, Alewine C, Le DT, Jaffee EM, Pastan I. Mesothelin Immunotherapy for Cancer: Ready for Prime Time? J Clin Oncol 2016; 34(34):4171-4179; PMID:27863199; https://doi.org/10.1200/JCO.2016.68.3672
   PubMed Web of Science ®Google Scholar
• Gajewiak J, Azam L, Imperial J, Walewska A, Green BR, Bandyopadhyay PK, Raghuraman S, Ueberheide B, Bern M, Zhou HM, et al. A disulfide tether stabilizes the block of sodium channels by the conotoxin μO§-GVIIJ. Proc Natl Acad Sci U S A 2014; 111(7):2758-63; PMID:24497506; https://doi.org/10.1073/pnas.1324189111
   PubMed Web of Science ®Google Scholar
• McSherry T, McSherry J, Ozaeta P, Longenecker K, Ramsay C, Fishpaugh J, Allen S. Cysteinylation of a monoclonal antibody leads to its inactivation. MAbs 2016; 8(4):718-25; PMID:27050640; https://doi.org/10.1080/19420862.2016.1160179
   PubMed Web of Science ®Google Scholar
• Shopes B. A genetically engineered human IgG mutant with enhanced cytolytic activity. J Immunol 1992; 148(9):2918-22; PMID:1573276
   PubMed Web of Science ®Google Scholar
• Shen BQ, Xu K, Liu L, Raab H, Bhakta S, Kenrick M, Parsons-Reponte KL, Tien J, Yu SF, Mai E, et al. Conjugation site modulates the in vivo stability and therapeutic activity of antibody-drug conjugates. Nat Biotechnol 2012; 30(2):184-9; PMID:22267010; https://doi.org/10.1038/nbt.2108
   PubMed Web of Science ®Google Scholar
• Arai H, Glabe C, Luecke H. Crystal structure of a conformation-dependent rabbit IgG Fab specific for amyloid prefibrillar oligomers. Biochim Biophys Acta 2012; 1820(12):1908-14; PMID:22940003; https://doi.org/10.1016/j.bbagen.2012.08.016
   PubMed Web of Science ®Google Scholar
• Pan R, Sampson JM, Chen Y, Vaine M, Wang S, Lu S, Kong XP. Rabbit anti-HIV-1 monoclonal antibodies raised by immunization can mimic the antigen-binding modes of antibodies derived from HIV-1-infected humans. J Virol 2013; 87(18):10221-31; PMID:23864637; https://doi.org/10.1128/JVI.00843-13
   PubMed Web of Science ®Google Scholar
• Pan R, Chen Y, Vaine M, Hu G, Wang S, Lu S, Kong XP. Structural analysis of a novel rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region critical for receptor and co-receptor binding. Emerg Microbes Infect 2015; 4(7):e44; PMID:26251831; https://doi.org/10.1038/emi.2015.44
   PubMed Web of Science ®Google Scholar
• Zhai Q, He M, Song A, Deshayes K, Dixit VM, Carter PJ. Structural Analysis and Optimization of Context-Independent Anti-Hypusine Antibodies. J Mol Biol 2016; 428(3):603-17; PMID:26778617; https://doi.org/10.1016/j.jmb.2016.01.006
   PubMed Web of Science ®Google Scholar
• Malia TJ, Teplyakov A, Brezski RJ, Luo J, Kinder M, Sweet RW, Almagro JC, Jordan RE, Gilliland GL. Structure and specificity of an antibody targeting a proteolytically cleaved IgG hinge. Proteins 2014; 82(8):1656-67; PMID:24638881; https://doi.org/10.1002/prot.24545
   PubMed Web of Science ®Google Scholar
• Bujotzek A, Lipsmeier F, Harris SF, Benz J, Kuglstatter A, Georges G. VH-VL orientation prediction for antibody humanization candidate selection: A case study. MAbs 2016; 8(2):288-305; PMID:26637054; https://doi.org/10.1080/19420862.2015.1117720
   PubMed Web of Science ®Google Scholar
• Shinmi D, Taguchi E, Iwano J, Yamaguchi T, Masuda K, Enokizono J, Shiraishi Y. One-Step Conjugation Method for Site-Specific Antibody-Drug Conjugates through Reactive Cysteine-Engineered Antibodies. Bioconjug Chem 2016; 27(5):1324-31; PMID:27074832; https://doi.org/10.1021/acs.bioconjchem.6b00133
   PubMed Web of Science ®Google Scholar
• Ramil CP, Lin Q. Bioorthogonal chemistry: strategies and recent developments. Chem Commun (Camb) 2013; 49(94):11007-22; PMID:24145483; https://doi.org/10.1039/c3cc44272a
   PubMed Web of Science ®Google Scholar
• Zhang YF, Ho M. Humanization of rabbit monoclonal antibodies via grafting combined Kabat/IMGT/Paratome complementarity-determining regions: Rationale and examples. MAbs 2017; 9:1-11; PMID:28165915; https://doi.org/10.1080/19420862.2017.1289302
   PubMed Web of Science ®Google Scholar
• Spidel JL, Albone E, Cheng X, Park YC, Vaessen B, Jacob S, Milinichik A, Verdi A, Kline JB, and Grasso L. Engineering humanized antibody framework sequences for optimal site-specific conjugation of cytotoxins. MAbs 2017; ( in press).
   PubMedGoogle Scholar
• Niedbala WG, Stott DI. A comparison of three methods for production of human hybridomas secreting autoantibodies. Hybridoma 1998; 17(3):299-304; PMID:9708833; https://doi.org/10.1089/hyb.1998.17.299
   PubMedGoogle Scholar
• Spidel JL, Vaessen B, Chan YY, Grasso L, Kline JB. Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells. J Immunol Methods 2016; 439:50-8; PMID:27677581; https://doi.org/10.1016/j.jim.2016.09.007
   PubMed Web of Science ®Google Scholar