Transcription elongation control by the 7SK snRNP complex: Releasing the pause

References

• Ljungman M. The transcription stress response. Cell Cycle 2007; 6:2252-7; PMID:17700065; http://dx.doi.org/10.4161/cc.6.18.4751.
   PubMed Web of Science ®Google Scholar
• Nechaev S, Adelman K. Promoter-proximal Pol II: when stalling speeds things up. Cell Cycle 2008; 7:1539-44; PMID:18469524; http://dx.doi.org/10.4161/cc.7.11.6006
   PubMed Web of Science ®Google Scholar
• Danko CG, Hah N, Luo X, Martins AL, Core L, Lis JT, Siepel A, Kraus WL. Signaling pathways differentially affect RNA polymerase II initiation, pausing, and elongation rate in cells. Mol Cell 2013; 50:212-22; PMID:23523369; http://dx.doi.org/10.1016/j.molcel.2013.02.015
   PubMed Web of Science ®Google Scholar
• Henriques T, Gilchrist DA, Nechaev S, Bern M, Muse GW, Burkholder A, Fargo DC, Adelman K. Stable pausing by RNA polymerase II provides an opportunity to target and integrate regulatory signals. Mol Cell 2013; 52:517-28; PMID:24184211; http://dx.doi.org/10.1016/j.molcel.2013.10.001
   PubMed Web of Science ®Google Scholar
• Muse GW, Gilchrist DA, Nechaev S, Shah R, Parker JS, Grissom SF, Zeitlinger J, Adelman K. RNA polymerase is poised for activation across the genome. Nat Genet 2007; 39:1507-11; PMID:17994021; http://dx.doi.org/10.1038/ng.2007.21
   PubMed Web of Science ®Google Scholar
• Zeitlinger J, Stark A, Kellis M, Hong JW, Nechaev S, Adelman K, Levine M, Young RA. RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryo. Nat Genet 2007; 39:1512-6; PMID:17994019; http://dx.doi.org/10.1038/ng.2007.26
   PubMed Web of Science ®Google Scholar
• Baugh LR, Demodena J, Sternberg PW. RNA Pol II accumulates at promoters of growth genes during developmental arrest. Science 2009; 324:92-4; PMID:19251593; http://dx.doi.org/10.1126/science.1169628
   PubMed Web of Science ®Google Scholar
• Rahl PB, Lin CY, Seila AC, Flynn RA, McCuine S, Burge CB, Sharp PA, Young RA. c-Myc regulates transcriptional pause release. Cell 2010; 141:432-45; PMID:20434984; http://dx.doi.org/10.1016/j.cell.2010.03.030
   PubMed Web of Science ®Google Scholar
• Adelman K, Lis JT. Promoter-proximal pausing of RNA polymerase II: emerging roles in metazoans. Nat Rev Genet 2012; 13:720-31; PMID:22986266; http://dx.doi.org/10.1038/nrg3293
   PubMed Web of Science ®Google Scholar
• Zhou Q, Li T, Price DH. RNA polymerase II elongation control. Annu Rev Biochem 2012; 81:119-43; PMID:22404626; http://dx.doi.org/10.1146/annurev-biochem-052610-095910
   PubMed Web of Science ®Google Scholar
• Jonkers I, Lis JT. Getting up to speed with transcription elongation by RNA polymerase II. Nat Rev Mol Cell Biol 2015; 16:167-77; PMID:25693130; http://dx.doi.org/10.1038/nrm3953
   PubMed Web of Science ®Google Scholar
• Yamaguchi Y, Takagi T, Wada T, Yano K, Furuya A, Sugimoto S, Hasegawa J, Handa H. NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation. Cell 1999; 97:41-51; PMID:10199401; http://dx.doi.org/10.1016/S0092-8674(00)80713-8
   PubMed Web of Science ®Google Scholar
• Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, et al. DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs. Genes Dev 1998; 12:343-56; PMID:9450929; http://dx.doi.org/10.1101/gad.12.3.343
   PubMed Web of Science ®Google Scholar
• Wada T, Takagi T, Yamaguchi Y, Watanabe D, Handa H. Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro. EMBO J 1998; 17:7395-403; PMID:9857195; http://dx.doi.org/10.1093/emboj/17.24.7395
   PubMed Web of Science ®Google Scholar
• Wu CH, Yamaguchi Y, Benjamin LR, Horvat-Gordon M, Washinsky J, Enerly E, Larsson J, Lambertsson A, Handa H, Gilmour D. NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila. Genes Dev 2003; 17:1402-14; PMID:12782658; http://dx.doi.org/10.1101/gad.1091403
   PubMed Web of Science ®Google Scholar
• Saunders A, Core LJ, Lis JT. Breaking barriers to transcription elongation. Nat Rev Mol Cell Biol 2006; 7:557-67; PMID:16936696; http://dx.doi.org/10.1038/nrm1981
   PubMed Web of Science ®Google Scholar
• Fuda NJ, Ardehali MB, Lis JT. Defining mechanisms that regulate RNA polymerase II transcription in vivo. Nature 2009; 461:186-92; PMID:19741698; http://dx.doi.org/10.1038/nature08449
   PubMed Web of Science ®Google Scholar
• Gilchrist DA, Dos Santos G, Fargo DC, Xie B, Gao Y, Li L, Adelman K. Pausing of RNA polymerase II disrupts DNA-specified nucleosome organization to enable precise gene regulation. Cell 2010; 143:540-51; PMID:21074046; http://dx.doi.org/10.1016/j.cell.2010.10.004
   PubMed Web of Science ®Google Scholar
• Gilchrist DA, Nechaev S, Lee C, Ghosh SK, Collins JB, Li L, Gilmour DS, Adelman K. NELF-mediated stalling of Pol II can enhance gene expression by blocking promoter-proximal nucleosome assembly. Genes Dev 2008; 22:1921-33; PMID:18628398; http://dx.doi.org/10.1101/gad.1643208
   PubMed Web of Science ®Google Scholar
• Mancebo HS, Lee G, Flygare J, Tomassini J, Luu P, Zhu Y, Peng J, Blau C, Hazuda D, Price D, et al. P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro. Genes Dev 1997; 11:2633-44; PMID:9334326; http://dx.doi.org/10.1101/gad.11.20.2633
   PubMed Web of Science ®Google Scholar
• Barboric M, Nissen RM, Kanazawa S, Jabrane-Ferrat N, Peterlin BM. NF-kappaB binds P-TEFb to stimulate transcriptional elongation by RNA polymerase II. Mol Cell 2001; 8:327-37; PMID:11545735; http://dx.doi.org/10.1016/S1097-2765(01)00314-8
   PubMed Web of Science ®Google Scholar
• Gomes NP, Bjerke G, Llorente B, Szostek SA, Emerson BM, Espinosa JM. Gene-specific requirement for P-TEFb activity and RNA polymerase II phosphorylation within the p53 transcriptional program. Genes Dev 2006; 20:601-12; PMID:16510875; http://dx.doi.org/10.1101/gad.1398206
   PubMed Web of Science ®Google Scholar
• Lis JT, Mason P, Peng J, Price DH, Werner J. P-TEFb kinase recruitment and function at heat shock loci. Genes Dev 2000; 14:792-803; PMID:10766736
   PubMed Web of Science ®Google Scholar
• McNamara RP, McCann JL, Gudipaty SA, D'Orso I. Transcription factors mediate the enzymatic disassembly of promoter-bound 7SK snRNP to locally recruit P-TEFb for transcription elongation. Cell Rep 2013; 5:1256-68; PMID:24316072; http://dx.doi.org/10.1016/j.celrep.2013.11.003
   PubMed Web of Science ®Google Scholar
• Zobeck KL, Buckley MS, Zipfel WR, Lis JT. Recruitment timing and dynamics of transcription factors at the Hsp70 loci in living cells. Mol Cell 2010; 40:965-75; PMID:21172661; http://dx.doi.org/10.1016/j.molcel.2010.11.022
   PubMed Web of Science ®Google Scholar
• Peterlin BM, Price DH. Controlling the elongation phase of transcription with P-TEFb. Mol Cell 2006; 23:297-305; PMID:16885020; http://dx.doi.org/10.1016/j.molcel.2006.06.014
   PubMed Web of Science ®Google Scholar
• Chao SH, Price DH. Flavopiridol inactivates P-TEFb and blocks most RNA polymerase II transcription in vivo. J Biol Chem 2001; 276:31793-9; PMID:11431468; http://dx.doi.org/10.1074/jbc.M102306200
   PubMed Web of Science ®Google Scholar
• Laitem C, Zaborowska J, Isa NF, Kufs J, Dienstbier M, Murphy S. CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes. Nat Struct Mol Biol 2015; 22:396-403; PMID:25849141
   PubMed Web of Science ®Google Scholar
• Hargreaves DC, Horng T, Medzhitov R. Control of inducible gene expression by signal-dependent transcriptional elongation. Cell 2009; 138:129-45; PMID:19596240; http://dx.doi.org/10.1016/j.cell.2009.05.047
   PubMed Web of Science ®Google Scholar
• McNamara RP, Reeder JE, McMillan EA, Bacon CW, McCann JL, D'Orso I. KAP1 Recruitment of the 7SK snRNP Complex to Promoters Enables Transcription Elongation by RNA Polymerase II. Mol Cell 2016; 61:39-53; PMID:26725010; http://dx.doi.org/10.1016/j.molcel.2015.11.004
   PubMed Web of Science ®Google Scholar
• D'Orso I, Frankel AD. RNA-mediated displacement of an inhibitory snRNP complex activates transcription elongation. Nat Struct Mol Biol 2010; 17:815-21; PMID:20562857; http://dx.doi.org/10.1038/nsmb.1827
   PubMed Web of Science ®Google Scholar
• Ji X, Zhou Y, Pandit S, Huang J, Li H, Lin CY, Xiao R, Burge CB, Fu XD. SR proteins collaborate with 7SK and promoter-associated nascent RNA to release paused polymerase. Cell 2013; 153:855-68; PMID:23663783; http://dx.doi.org/10.1016/j.cell.2013.04.028
   PubMed Web of Science ®Google Scholar
• Calo E, Flynn RA, Martin L, Spitale RC, Chang HY, Wysocka J. RNA helicase DDX21 coordinates transcription and ribosomal RNA processing. Nature 2015; 518:249-53; PMID:25470060; http://dx.doi.org/10.1038/nature13923
   PubMed Web of Science ®Google Scholar
• Liu W, Ma Q, Wong K, Li W, Ohgi K, Zhang J, Aggarwal AK, Rosenfeld MG. Brd4 and JMJD6-associated anti-pause enhancers in regulation of transcriptional pause release. Cell 2013; 155:1581-95; PMID:24360279; http://dx.doi.org/10.1016/j.cell.2013.10.056
   PubMed Web of Science ®Google Scholar
• Flynn RA, Do BT, Rubin AJ, Calo E, Lee B, Kuchelmeister H, Rale M, Chu C, Kool ET, Wysocka J, et al. 7SK-BAF axis controls pervasive transcription at enhancers. Nat Struct Mol Biol 2016; 23:231-8; PMID:26878240; http://dx.doi.org/10.1038/nsmb.3176
   PubMed Web of Science ®Google Scholar
• Cherrier T, Le Douce V, Eilebrecht S, Riclet R, Marban C, Dequiedt F, Goumon Y, Paillart JC, Mericskay M, Parlakian A, et al. CTIP2 is a negative regulator of P-TEFb. Proc Natl Acad Sci U S A 2013; 110:12655-60; PMID:23852730; http://dx.doi.org/10.1073/pnas.1220136110
   PubMed Web of Science ®Google Scholar
• Gudipaty SA, McNamara RP, Morton EL, D'Orso I. PPM1G Binds 7SK RNA and Hexim1 To Block P-TEFb Assembly into the 7SK snRNP and Sustain Transcription Elongation. Mol Cell Biol 2015; 35:3810-28; PMID:26324325; http://dx.doi.org/10.1128/MCB.00226-15
   PubMed Web of Science ®Google Scholar
• Peng J, Zhu Y, Milton JT, Price DH. Identification of multiple cyclin subunits of human P-TEFb. Genes Dev 1998; 12:755-62; PMID:9499409; http://dx.doi.org/10.1101/gad.12.5.755
   PubMed Web of Science ®Google Scholar
• Cho S, Schroeder S, Ott M. CYCLINg through transcription: posttranslational modifications of P-TEFb regulate transcription elongation. Cell Cycle 2010; 9:1697-705; PMID:20436276; http://dx.doi.org/10.4161/cc.9.9.11346
   PubMed Web of Science ®Google Scholar
• Wei P, Garber ME, Fang SM, Fischer WH, Jones KA. A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA. Cell 1998; 92:451-62; PMID:9491887; http://dx.doi.org/10.1016/S0092-8674(00)80939-3
   PubMed Web of Science ®Google Scholar
• McCracken S, Fong N, Yankulov K, Ballantyne S, Pan G, Greenblatt J, Patterson SD, Wickens M, Bentley DL. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription. Nature 1997; 385:357-61; PMID:9002523; http://dx.doi.org/10.1038/385357a0
   PubMed Web of Science ®Google Scholar
• Eick D, Geyer M. The RNA polymerase II carboxy-terminal domain (CTD) code. Chem Rev 2013; 113:8456-90; PMID:23952966; http://dx.doi.org/10.1021/cr400071f
   PubMed Web of Science ®Google Scholar
• Narita T, Yamaguchi Y, Yano K, Sugimoto S, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, et al. Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex. Mol Cell Biol 2003; 23:1863-73; PMID:12612062; http://dx.doi.org/10.1128/MCB.23.6.1863-1873.2003
   PubMed Web of Science ®Google Scholar
• Yamada T, Yamaguchi Y, Inukai N, Okamoto S, Mura T, Handa H. P-TEFb-mediated phosphorylation of hSpt5 C-terminal repeats is critical for processive transcription elongation. Mol Cell 2006; 21:227-37; PMID:16427012; http://dx.doi.org/10.1016/j.molcel.2005.11.024
   PubMed Web of Science ®Google Scholar
• Pirngruber J, Shchebet A, Johnsen SA. Insights into the function of the human P-TEFb component CDK9 in the regulation of chromatin modifications and co-transcriptional mRNA processing. Cell Cycle 2009; 8:3636-42; PMID:19844166; http://dx.doi.org/10.4161/cc.8.22.9890
   PubMed Web of Science ®Google Scholar
• Patel MC, Debrosse M, Smith M, Dey A, Huynh W, Sarai N, Heightman TD, Tamura T, Ozato K. BRD4 coordinates recruitment of pause release factor P-TEFb and the pausing complex NELF/DSIF to regulate transcription elongation of interferon-stimulated genes. Mol Cell Biol 2013; 33:2497-507; PMID:23589332; http://dx.doi.org/10.1128/MCB.01180-12
   PubMed Web of Science ®Google Scholar
• Fujinaga K, Irwin D, Huang Y, Taube R, Kurosu T, Peterlin BM. Dynamics of human immunodeficiency virus transcription: P-TEFb phosphorylates RD and dissociates negative effectors from the transactivation response element. Mol Cell Biol 2004; 24:787-95; PMID:14701750; http://dx.doi.org/10.1128/MCB.24.2.787-795.2004
   PubMed Web of Science ®Google Scholar
• Missra A, Gilmour DS. Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Proc Natl Acad Sci U S A 2010; 107:11301-6; PMID:20534440; http://dx.doi.org/10.1073/pnas.1000681107
   PubMed Web of Science ®Google Scholar
• He N, Liu M, Hsu J, Xue Y, Chou S, Burlingame A, Krogan NJ, Alber T, Zhou Q. HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription. Mol Cell 2010; 38:428-38; PMID:20471948; http://dx.doi.org/10.1016/j.molcel.2010.04.013
   PubMed Web of Science ®Google Scholar
• Lin C, Smith ER, Takahashi H, Lai KC, Martin-Brown S, Florens L, Washburn MP, Conaway JW, Conaway RC, Shilatifard A. AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia. Mol Cell 2010; 37:429-37; PMID:20159561; http://dx.doi.org/10.1016/j.molcel.2010.01.026
   PubMed Web of Science ®Google Scholar
• Luo Z, Lin C, Shilatifard A. The super elongation complex (SEC) family in transcriptional control. Nat Rev Mol Cell Biol 2012; 13:543-7; PMID:22895430; http://dx.doi.org/10.1038/nrm3417
   PubMed Web of Science ®Google Scholar
• Hsin JP, Li W, Hoque M, Tian B, Manley JL. RNAP II CTD tyrosine 1 performs diverse functions in vertebrate cells. Elife 2014; 3:e02112; PMID:24842995; http://dx.doi.org/10.7554/eLife.02112
   PubMed Web of Science ®Google Scholar
• Descostes N, Heidemann M, Spinelli L, Schuller R, Maqbool MA, Fenouil R, Koch F, Innocenti C, Gut M, Gut I, et al. Tyrosine phosphorylation of RNA polymerase II CTD is associated with antisense promoter transcription and active enhancers in mammalian cells. Elife 2014; 3:e02105; PMID:24842994; http://dx.doi.org/10.7554/eLife.02105
   PubMed Web of Science ®Google Scholar
• Mayer A, Heidemann M, Lidschreiber M, Schreieck A, Sun M, Hintermair C, Kremmer E, Eick D, Cramer P. CTD tyrosine phosphorylation impairs termination factor recruitment to RNA polymerase II. Science 2012; 336:1723-5; PMID:22745433; http://dx.doi.org/10.1126/science.1219651
   PubMed Web of Science ®Google Scholar
• Buratowski S. Progression through the RNA polymerase II CTD cycle. Mol Cell 2009; 36:541-6; PMID:19941815; http://dx.doi.org/10.1016/j.molcel.2009.10.019
   PubMed Web of Science ®Google Scholar
• Czudnochowski N, Bosken CA, Geyer M. Serine-7 but not serine-5 phosphorylation primes RNA polymerase II CTD for P-TEFb recognition. Nat Commun 2012; 3:842; PMID:22588304; http://dx.doi.org/10.1038/ncomms1846
   PubMed Web of Science ®Google Scholar
• Suh H, Ficarro SB, Kang UB, Chun Y, Marto JA, Buratowski S. Direct Analysis of Phosphorylation Sites on the Rpb1 C-Terminal Domain of RNA Polymerase II. Mol Cell 2016; 61:297-304; PMID:26799764; http://dx.doi.org/10.1016/j.molcel.2015.12.021
   PubMed Web of Science ®Google Scholar
• Schuller R, Forne I, Straub T, Schreieck A, Texier Y, Shah N, Decker TM, Cramer P, Imhof A, Eick D. Heptad-Specific Phosphorylation of RNA Polymerase II CTD. Mol Cell 2016; 61:305-14; PMID:26799765; http://dx.doi.org/10.1016/j.molcel.2015.12.003
   PubMed Web of Science ®Google Scholar
• Eberhardy SR, Farnham PJ. Myc recruits P-TEFb to mediate the final step in the transcriptional activation of the cad promoter. J Biol Chem 2002; 277:40156-62; PMID:12177005; http://dx.doi.org/10.1074/jbc.M207441200
   PubMed Web of Science ®Google Scholar
• Tahirov TH, Babayeva ND, Varzavand K, Cooper JJ, Sedore SC, Price DH. Crystal structure of HIV-1 Tat complexed with human P-TEFb. Nature 2010; 465:747-51; PMID:20535204; http://dx.doi.org/10.1038/nature09131
   PubMed Web of Science ®Google Scholar
• Zhou M, Halanski MA, Radonovich MF, Kashanchi F, Peng J, Price DH, Brady JN. Tat modifies the activity of CDK9 to phosphorylate serine 5 of the RNA polymerase II carboxyl-terminal domain during human immunodeficiency virus type 1 transcription. Mol Cell Biol 2000; 20:5077-86; PMID:10866664; http://dx.doi.org/10.1128/MCB.20.14.5077-5086.2000
   PubMed Web of Science ®Google Scholar
• Nguyen VT, Kiss T, Michels AA, Bensaude O. 7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes. Nature 2001; 414:322-5; PMID:11713533; http://dx.doi.org/10.1038/35104581
   PubMed Web of Science ®Google Scholar
• Yang Z, Zhu Q, Luo K, Zhou Q. The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription. Nature 2001; 414:317-22; PMID:11713532; http://dx.doi.org/10.1038/35104575
   PubMed Web of Science ®Google Scholar
• Barboric M, Lenasi T, Chen H, Johansen EB, Guo S, Peterlin BM. 7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development. Proc Natl Acad Sci U S A 2009; 106:7798-803; PMID:19416841; http://dx.doi.org/10.1073/pnas.0903188106
   PubMed Web of Science ®Google Scholar
• Wassarman DA, Steitz JA. Structural analyses of the 7SK ribonucleoprotein (RNP), the most abundant human small RNP of unknown function. Mol Cell Biol 1991; 11:3432-45; PMID:1646389; http://dx.doi.org/10.1128/MCB.11.7.3432
   PubMed Web of Science ®Google Scholar
• Muniz L, Egloff S, Kiss T. RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP. Nucleic Acids Res 2013; 41:4686-98; PMID:23471002; http://dx.doi.org/10.1093/nar/gkt159
   PubMed Web of Science ®Google Scholar
• Markert A, Grimm M, Martinez J, Wiesner J, Meyerhans A, Meyuhas O, Sickmann A, Fischer U. The La-related protein LARP7 is a component of the 7SK ribonucleoprotein and affects transcription of cellular and viral polymerase II genes. EMBO Rep 2008; 9:569-75; PMID:18483487; http://dx.doi.org/10.1038/embor.2008.72
   PubMed Web of Science ®Google Scholar
• Li Q, Price JP, Byers SA, Cheng D, Peng J, Price DH. Analysis of the large inactive P-TEFb complex indicates that it contains one 7SK molecule, a dimer of HEXIM1 or HEXIM2, and two P-TEFb molecules containing Cdk9 phosphorylated at threonine 186. J Biol Chem 2005; 280:28819-26; PMID:15965233; http://dx.doi.org/10.1074/jbc.M502712200
   PubMed Web of Science ®Google Scholar
• Yik JH, Chen R, Nishimura R, Jennings JL, Link AJ, Zhou Q. Inhibition of P-TEFb (CDK9/Cyclin T) kinase and RNA polymerase II transcription by the coordinated actions of HEXIM1 and 7SK snRNA. Mol Cell 2003; 12:971-82; PMID:14580347; http://dx.doi.org/10.1016/S1097-2765(03)00388-5
   PubMed Web of Science ®Google Scholar
• Yik JH, Chen R, Pezda AC, Zhou Q. Compensatory contributions of HEXIM1 and HEXIM2 in maintaining the balance of active and inactive positive transcription elongation factor b complexes for control of transcription. J Biol Chem 2005; 280:16368-76; PMID:15713661; http://dx.doi.org/10.1074/jbc.M500912200
   PubMed Web of Science ®Google Scholar
• He N, Jahchan NS, Hong E, Li Q, Bayfield MA, Maraia RJ, Luo K, Zhou Q. A La-related protein modulates 7SK snRNP integrity to suppress P-TEFb-dependent transcriptional elongation and tumorigenesis. Mol Cell 2008; 29:588-99; PMID:18249148; http://dx.doi.org/10.1016/j.molcel.2008.01.003
   PubMed Web of Science ®Google Scholar
• Krueger BJ, Jeronimo C, Roy BB, Bouchard A, Barrandon C, Byers SA, Searcey CE, Cooper JJ, Bensaude O, Cohen EA, et al. LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated. Nucleic Acids Res 2008; 36:2219-29; PMID:18281698; http://dx.doi.org/10.1093/nar/gkn061
   PubMed Web of Science ®Google Scholar
• Jeronimo C, Forget D, Bouchard A, Li Q, Chua G, Poitras C, Therien C, Bergeron D, Bourassa S, Greenblatt J, et al. Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Mol Cell 2007; 27:262-74; PMID:17643375; http://dx.doi.org/10.1016/j.molcel.2007.06.027
   PubMed Web of Science ®Google Scholar
• Xue Y, Yang Z, Chen R, Zhou Q. A capping-independent function of MePCE in stabilizing 7SK snRNA and facilitating the assembly of 7SK snRNP. Nucleic Acids Res 2010; 38:360-9; PMID:19906723; http://dx.doi.org/10.1093/nar/gkp977
   PubMed Web of Science ®Google Scholar
• Muniz L, Egloff S, Ughy B, Jady BE, Kiss T. Controlling cellular P-TEFb activity by the HIV-1 transcriptional transactivator Tat. PLoS Pathog 2010; 6:e1001152; PMID:20976203; http://dx.doi.org/10.1371/journal.ppat.1001152
   PubMedGoogle Scholar
• Egloff S, Van Herreweghe E, Kiss T. Regulation of polymerase II transcription by 7SK snRNA: two distinct RNA elements direct P-TEFb and HEXIM1 binding. Mol Cell Biol 2006; 26:630-42; PMID:16382153; http://dx.doi.org/10.1128/MCB.26.2.630-642.2006
   PubMed Web of Science ®Google Scholar
• Michels AA, Fraldi A, Li Q, Adamson TE, Bonnet F, Nguyen VT, Sedore SC, Price JP, Price DH, Lania L, et al. Binding of the 7SK snRNA turns the HEXIM1 protein into a P-TEFb (CDK9/cyclin T) inhibitor. EMBO J 2004; 23:2608-19; PMID:15201869; http://dx.doi.org/10.1038/sj.emboj.7600275
   PubMed Web of Science ®Google Scholar
• Barboric M, Kohoutek J, Price JP, Blazek D, Price DH, Peterlin BM. Interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct the inhibition of P-TEFb. EMBO J 2005; 24:4291-303; PMID:16362050; http://dx.doi.org/10.1038/sj.emboj.7600883
   PubMed Web of Science ®Google Scholar
• Blazek D, Barboric M, Kohoutek J, Oven I, Peterlin BM. Oligomerization of HEXIM1 via 7SK snRNA and coiled-coil region directs the inhibition of P-TEFb. Nucleic Acids Res 2005; 33:7000-10; PMID:16377779; http://dx.doi.org/10.1093/nar/gki997
   PubMed Web of Science ®Google Scholar
• Schulte A, Czudnochowski N, Barboric M, Schonichen A, Blazek D, Peterlin BM, Geyer M. Identification of a cyclin T-binding domain in Hexim1 and biochemical analysis of its binding competition with HIV-1 Tat. J Biol Chem 2005; 280:24968-77; PMID:15855166; http://dx.doi.org/10.1074/jbc.M501431200
   PubMed Web of Science ®Google Scholar
• Dames SA, Schonichen A, Schulte A, Barboric M, Peterlin BM, Grzesiek S, Geyer M. Structure of the Cyclin T binding domain of Hexim1 and molecular basis for its recognition of P-TEFb. Proc Natl Acad Sci U S A 2007; 104:14312-7; PMID:17724342; http://dx.doi.org/10.1073/pnas.0701848104
   PubMed Web of Science ®Google Scholar
• Chen R, Yang Z, Zhou Q. Phosphorylated positive transcription elongation factor b (P-TEFb) is tagged for inhibition through association with 7SK snRNA. J Biol Chem 2004; 279:4153-60; PMID:14627702; http://dx.doi.org/10.1074/jbc.M310044200
   PubMed Web of Science ®Google Scholar
• Fisher RP. The CDK Network: Linking Cycles of Cell Division and Gene Expression. Genes Cancer 2012; 3:731-8; PMID:23634260; http://dx.doi.org/10.1177/1947601912473308
   PubMedGoogle Scholar
• Garber ME, Mayall TP, Suess EM, Meisenhelder J, Thompson NE, Jones KA. CDK9 autophosphorylation regulates high-affinity binding of the human immunodeficiency virus type 1 tat-P-TEFb complex to TAR RNA. Mol Cell Biol 2000; 20:6958-69; PMID:10958691; http://dx.doi.org/10.1128/MCB.20.18.6958-6969.2000
   PubMed Web of Science ®Google Scholar
• Larochelle S, Amat R, Glover-Cutter K, Sanso M, Zhang C, Allen JJ, Shokat KM, Bentley DL, Fisher RP. Cyclin-dependent kinase control of the initiation-to-elongation switch of RNA polymerase II. Nat Struct Mol Biol 2012; 19:1108-15; PMID:23064645; http://dx.doi.org/10.1038/nsmb.2399
   PubMed Web of Science ®Google Scholar
• Chen R, Liu M, Li H, Xue Y, Ramey WN, He N, Ai N, Luo H, Zhu Y, Zhou N, et al. PP2B and PP1alpha cooperatively disrupt 7SK snRNP to release P-TEFb for transcription in response to Ca2+ signaling. Genes Dev 2008; 22:1356-68; PMID:18483222; http://dx.doi.org/10.1101/gad.1636008
   PubMed Web of Science ®Google Scholar
• Wang Y, Dow EC, Liang YY, Ramakrishnan R, Liu H, Sung TL, Lin X, Rice AP. Phosphatase PPM1A regulates phosphorylation of Thr-186 in the Cdk9 T-loop. J Biol Chem 2008; 283:33578-84; PMID:18829461; http://dx.doi.org/10.1074/jbc.M807495200
   PubMed Web of Science ®Google Scholar
• D'Orso I, Jang GM, Pastuszak AW, Faust TB, Quezada E, Booth DS, Frankel AD. Transition step during assembly of HIV Tat:P-TEFb transcription complexes and transfer to TAR RNA. Mol Cell Biol 2012; 32:4780-93; PMID:23007159; http://dx.doi.org/10.1128/MCB.00206-12
   PubMed Web of Science ®Google Scholar
• Iyengar S, Farnham PJ. KAP1 protein: an enigmatic master regulator of the genome. J Biol Chem 2011; 286:26267-76; PMID:21652716; http://dx.doi.org/10.1074/jbc.R111.252569
   PubMed Web of Science ®Google Scholar
• Iyengar S, Ivanov AV, Jin VX, Rauscher FJ, 3rd, Farnham PJ. Functional analysis of KAP1 genomic recruitment. Mol Cell Biol 2011; 31:1833-47; PMID:21343339; http://dx.doi.org/10.1128/MCB.01331-10
   PubMed Web of Science ®Google Scholar
• Kim TK, Shiekhattar R. Architectural and Functional Commonalities between Enhancers and Promoters. Cell 2015; 162:948-59; PMID:26317464; http://dx.doi.org/10.1016/j.cell.2015.08.008
   PubMed Web of Science ®Google Scholar
• Van Herreweghe E, Egloff S, Goiffon I, Jady BE, Froment C, Monsarrat B, Kiss T. Dynamic remodelling of human 7SK snRNP controls the nuclear level of active P-TEFb. EMBO J 2007; 26:3570-80; PMID:17611602; http://dx.doi.org/10.1038/sj.emboj.7601783
   PubMed Web of Science ®Google Scholar
• Barrandon C, Bonnet F, Nguyen VT, Labas V, Bensaude O. The transcription-dependent dissociation of P-TEFb-HEXIM1-7SK RNA relies upon formation of hnRNP-7SK RNA complexes. Mol Cell Biol 2007; 27:6996-7006; PMID:17709395; http://dx.doi.org/10.1128/MCB.00975-07
   PubMed Web of Science ®Google Scholar
• Hogg JR, Collins K. RNA-based affinity purification reveals 7SK RNPs with distinct composition and regulation. RNA 2007; 13:868-80; PMID:17456562; http://dx.doi.org/10.1261/rna.565207
   PubMed Web of Science ®Google Scholar
• Mizutani T, Ishizaka A, Suzuki Y, Iba H. 7SK small nuclear ribonucleoprotein complex is recruited to the HIV-1 promoter via short viral transcripts. FEBS Lett 2014; 588:1630-6; PMID:24607481; http://dx.doi.org/10.1016/j.febslet.2014.01.067
   PubMed Web of Science ®Google Scholar
• Prasanth KV, Camiolo M, Chan G, Tripathi V, Denis L, Nakamura T, Hubner MR, Spector DL. Nuclear organization and dynamics of 7SK RNA in regulating gene expression. Mol Biol Cell 2010; 21:4184-96; PMID:20881057; http://dx.doi.org/10.1091/mbc.E10-02-0105
   PubMed Web of Science ®Google Scholar
• Bunch H, Zheng X, Burkholder A, Dillon ST, Motola S, Birrane G, Ebmeier CC, Levine S, Fargo D, Hu G, et al. TRIM28 regulates RNA polymerase II promoter-proximal pausing and pause release. Nat Struct Mol Biol 2014; 21:876-83; PMID:25173174; http://dx.doi.org/10.1038/nsmb.2878
   PubMed Web of Science ®Google Scholar
• Yang Z, Yik JH, Chen R, He N, Jang MK, Ozato K, Zhou Q. Recruitment of P-TEFb for stimulation of transcriptional elongation by the bromodomain protein Brd4. Mol Cell 2005; 19:535-45; PMID:16109377; http://dx.doi.org/10.1016/j.molcel.2005.06.029
   PubMed Web of Science ®Google Scholar
• Yang Z, He N, Zhou Q. Brd4 recruits P-TEFb to chromosomes at late mitosis to promote G1 gene expression and cell cycle progression. Mol Cell Biol 2008; 28:967-76; PMID:18039861; http://dx.doi.org/10.1128/MCB.01020-07
   PubMed Web of Science ®Google Scholar
• Jang MK, Mochizuki K, Zhou M, Jeong HS, Brady JN, Ozato K. The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription. Mol Cell 2005; 19:523-34; PMID:16109376; http://dx.doi.org/10.1016/j.molcel.2005.06.027
   PubMed Web of Science ®Google Scholar
• Itzen F, Greifenberg AK, Bosken CA, Geyer M. Brd4 activates P-TEFb for RNA polymerase II CTD phosphorylation. Nucleic Acids Res 2014; 42:7577-90; PMID:24860166; http://dx.doi.org/10.1093/nar/gku449
   PubMed Web of Science ®Google Scholar
• Bai X, Kim J, Yang Z, Jurynec MJ, Akie TE, Lee J, LeBlanc J, Sessa A, Jiang H, DiBiase A, et al. TIF1gamma controls erythroid cell fate by regulating transcription elongation. Cell 2010; 142:133-43; PMID:20603019; http://dx.doi.org/10.1016/j.cell.2010.05.028
   PubMed Web of Science ®Google Scholar