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Research Paper

An improved staining method of cell cycle analysis with Sybr Green I for fungi: Cryptococcus neoformans and Saccharomyces cerevisiae

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Pages 271-282 | Received 20 Nov 2019, Accepted 28 Dec 2020, Published online: 19 Jan 2021

References

  • Park BJ, Wannemuehler KA, Marston BJ, et al. Estimation of the current global burden of cryptococcal meningitis among persons living with HIV/AIDS. AIDS. 2009;23(4):525–530.
  • Srikanta D, Santiago-Tirado FH, Doering TL. Cryptococcus neoformans: historical curiosity to modern pathogen. Yeast. 2014;31:47–60.
  • Saijo T, Chen J, Chen SC, Rosen LB, Yi J, Sorrell TC, Bennett JE, Holland SM, Browne SK, Kwon-Chung KJ. Anti-granulocyte-macrophage colony-stimulating factor autoantibodies are a risk factor for central nervous system infection by Cryptococcus gattii in otherwise immunocompetent patients. mBio. 2014;5:e00912–14.
  • Gullo FP, Rossi SA, Sardi Jde C, et al. Cryptococcosis: epidemiology, fungal resistance, and new alternatives for treatment. Eur J Clin Microbiol Infect Dis. 2013;32:1377–1391.
  • Litvintseva AP, Carbone I, Rossouw J, et al. Evidence that the human pathogenic fungus Cryptococcus neoformans var. grubii may have evolved in Africa. PloS One. 2011;6:e19688.
  • Sloan DJ, Parris V. Cryptococcal meningitis: epidemiology and therapeutic options. Clin Epidemiol. 2014;6:169–182.
  • Mitchell TG, Perfect JR. Cryptococcosis in the era of AIDS–100 years after the discovery of Cryptococcus neoformans. Clin Microbiol Rev. 1995;8:515–548.
  • Chang CC, Sorrell TC, Chen SC. Pulmonary Cryptococcosis. Semin Respir Crit Care Med. 2015;36:681–691.
  • Barbosa FM, Fonseca FL, Figueiredo RT, et al. Binding of glucuronoxylomannan to the CD14 receptor in human A549 alveolar cells induces interleukin-8 production. Clin Vaccine Immunol. 2007;14:94–98.
  • Olszewski MA, Zhang Y, Huffnagle GB. Mechanisms of cryptococcal virulence and persistence. Future Microbiol. 2010;5:1269–1288.
  • Kozel TR, Gotschlich EC. The capsule of cryptococcus neoformans passively inhibits phagocytosis of the yeast by macrophages. J Immunol. 1982;129:1675–1680.
  • Zhu X, Williamson PR. Role of laccase in the biology and virulence of Cryptococcus neoformans. FEMS Yeast Res. 2004;5:1–10.
  • Perfect JR. Cryptococcus neoformans: the yeast that likes it hot. FEMS Yeast Res. 2006;6:463–468.
  • Cox GM, Mukherjee J, Cole GT, et al. Urease as a virulence factor in experimental cryptococcosis. Infect Immun. 2000;68:443–448.
  • Hull CM, Heitman J. Genetics of Cryptococcus neoformans. Annu Rev Genet. 2002;36:557–615.
  • Fang W, Price MS, Toffaletti DL, et al. Pleiotropic effects of deubiquitinating enzyme Ubp5 on growth and pathogenesis of Cryptococcus neoformans. PloS One. 2012;7:e38326.
  • Finley D, Sadis S, Monia BP, et al. Inhibition of proteolysis and cell cycle progression in a multiubiquitination-deficient yeast mutant. Mol Cell Biol. 1994;14:5501–5509.
  • Spitzer ED, Spitzer SG. Structure of the ubiquitin-encoding genes of Cryptococcus neoformans. Gene. 1995;161:113–117.
  • Fortuna M, Sousa MJ, Corte-Real M, et al. Cell cycle analysis of yeasts. Curr Protoc Cytom. 2001. Chapter 11: Unit11.3:11.13.1–11.13.9.
  • Buchanan KL, Murphy JW. What makes Cryptococcus neoformans a pathogen? Emerg Infect Dis. 1998;4:71–83.
  • Haase SB, Lew DJ. Flow cytometric analysis of DNA content in budding yeast. Methods Enzymol. 1997;283:322–332.
  • Haase SB, Reed SI. Improved flow cytometric analysis of the budding yeast cell cycle. Cell Cycle (Georgetown, Tex). 2002;1:132–136.
  • Li L, Miles S, Breeden LL. A Genetic Screen for Saccharomyces cerevisiae Mutants That Fail to Enter Quiescence. G3 (Bethesda). 2015;5:1783–1795.
  • Benson NA, Braylan RC. Evaluation of sensitivity in DNA aneuploidy detection using a mathematical model. Cytometry. 1994;15:53–58.
  • Townsend GF, Lindegren CC. Characteristic growth patterns of the different members of a polyploid series of Saccharomyces. J Bacteriol. 1954;67:480–483.

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