THE USE OF CONFOCAL LASER SCANNING MICROSCOPY TO ASSESS THE POTENTIAL SUITABILITY OF 3D SCAFFOLDS FOR TISSUE REGENERATION, BY MONITORING EXTRA‐CELLULAR MATRIX DEPOSITION AND BY QUANTIFYING CELLULAR INFILTRATION AND PROLIFERATION

REFERENCES

• Lamme , E. N. , Van Leeuwen , R. T. , Brandsma , K. , Van Marle , J. and Middelkoop , E. 2000 . Higher numbers of autologous fibroblasts in an artificial dermal substitute improve tissue regeneration and modulate scar tissue formation. . J. Pathol. , 190 : 595 – 603 .
   PubMed Web of Science ®Google Scholar
• Wang , H. J. , Pieper , J. , Schotel , R. , van Blitterswijk , C. A. and Lamme , E. N. 2004 . Stimulation of skin repair is dependent on fibroblast source and presence of extracellular matrix. . Tissue Eng. , 10 : 1054 – 1064 .
   Google Scholar
• Eckes , B. , Zigrino , P. , Kessler , D. , Holtkotter , O. , Shephard , P. and Mauch , C. 2000 . Fibroblast‐matrix interactions in wound healing and fibrosis. . Matrix Biol , 19 : 325 – 332 .
   PubMed Web of Science ®Google Scholar
• Ng , K. W. , Khor , H. L. , Hutmacher and D. W. 2004 . In vitro characterization of natural and synthetic dermal matrices cultured with human dermal fibroblasts. . Biomaterials , 25 : 2807 – 2818 .
   PubMed Web of Science ®Google Scholar
• Mosmann , T. 1983 . Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. . J. Immunol. Methods , 65 : 55 – 63 .
   PubMed Web of Science ®Google Scholar
• Ahmed , S. A. , Gogal , R. M. Jr. and Walsh , J. E. 1994 . A new rapid and simple non‐radioactive assay to monitor and determine the proliferation of lymphocytes: An alternative to [3H]thymidine incorporation assay. . J. Immunol. Methods , 170 : 211 – 224 .
   PubMed Web of Science ®Google Scholar
• Kangas , L. , Gronroos , M. and Nieminen , A. L. 1984 . Bioluminescence of cellular ATP: A new method for evaluating cytotoxic agents in vitro. . Med. Biol. , 62 : 338 – 343 .
   PubMedGoogle Scholar
• Crouch , S. P. , Kozlowski , R. , Slater , K. J. and Fletcher , J. 1993 . The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. . J. Immunol. Methods , 160 : 81 – 88 .
   PubMed Web of Science ®Google Scholar
• Mandal , B. B. and Kundu , S. C. 2008 . Non‐bioengineered silk fibroin protein 3‐D scaffolds for potential biotechnological and tissue engineering applications. . Macromol. Biosci. , 8 : 807 – 818 .
   PubMed Web of Science ®Google Scholar
• Rowe , S. L. and Stegemann , J. P. 2006 . Interpenetrating collagen‐fibrin composite matrices with varying protein contents and ratios. . Biomacromolecules , 7 : 2942 – 2948 .
   PubMed Web of Science ®Google Scholar
• Mahoney , M. J. and Anseth , K. S. 2006 . Three‐dimensional growth and function of neural tissue in degradable polyethylene glycol hydrogels. . Biomaterials , 27 : 2265 – 2274 .
   PubMed Web of Science ®Google Scholar
• Gilbert , T. W. , Freund , J. M. and Badylak , S. F. 2008 . Quantification of DNA in biologic scaffold materials. . J. Surg. Res. , 152 : 135 – 139 .
   Google Scholar
• Bopp , S. K. and Lettieri , T. 2008 . Comparison of four different colorimetric and fluorometric cytotoxicity assays in a zebrafish liver cell line. . B.M.C. Pharmacol. , 8 : 8
   Google Scholar
• Funk , D. , Schrenk , H. H. and Frei , E. 2007 . Serum albumin leads to false‐positive results in the XTT and the MTT assay. . Biotechniques , 43 : 178 – 186 .
   Google Scholar
• Vellonen , K. S. , Honkakoski , P. and Urtti , A. 2004 . Substrates and inhibitors of efflux proteins interfere with the MTT assay in cells and may lead to underestimation of drug toxicity. . Eur. J. Pharm. Sci. , 23 : 181 – 188 .
   PubMed Web of Science ®Google Scholar
• Cuckierman , E. , Pankov , R. and Yamada , K. M. 2002 . Cell interactions with three‐ dimensional matrices. . Curr. Opin. Cell Biol. , 14 : 633 – 639 .
   Google Scholar
• Lutolf , M. P. , Lauer‐Fields , J. L. , Schmoekel , H. G. , Metters , A. T. , Weber , F. E. and Fields , G. B. 2003 . Synthetic matrix metalloproteinase‐sensitive hydrogels for the conduction of tissue regeneration: Engineering cell‐invasion characteristics. . Proc. Natl. Acad. Sci. USA , 100 : 5413 – 5418 .
   PubMed Web of Science ®Google Scholar
• Jones , I. , James , S. E. , Rubin , P. and Martin , R. 2003 . Upward migration of cultured autologous keratinocytes in IntegraTM Artificial Skin: A preliminary report. . Wound Repair Regen. , 11 : 132 – 138 .
   Google Scholar
• Shevchenko , R. V. , Sibbons , P. D. , Sharpe , J. R. and James , S. E. 2008 . Use of a novel porcine collagen paste as a dermal substitute in full‐thickness wounds. . Wound Repair Regen. , 16 : 198 – 207 .
   Google Scholar
• Mao , Y. and Schwarzbauer , J. E. 2005 . Fibronectin fibrillogenesis, a cell‐mediated matrix assembly process. . Matrix Biol. , 24 : 389 – 399 .
   PubMed Web of Science ®Google Scholar
• Stamenkovic , I. 2003 . Extracellular matrix remodelling: The role of matrix metalloproteinases. . J. Pathol. , 200 : 448 – 464 .
   PubMed Web of Science ®Google Scholar
• Greiling , D. and Clark , R. A. F. 1997 . Fibronectin provides a conduit for fibroblast transmigration from collagenous stroma into fibrin clot provisional matrix. . J. Cell Sci. , 110 : 861 – 870 .
   PubMedGoogle Scholar
• Wierzbicka‐Patynowski , I. and Schwarzbauer , J. E. 2003 . The ins and outs of fibronectin assembly. . J. Cell Sci. , 116 : 3269 – 3276 .
   PubMed Web of Science ®Google Scholar
• Hinz , B. , Celetta , G. , Tomasek , J. J. , Gabbiani , G. and Chaponnier , C. 2001 . Alpha‐smooth muscle actin expression upregulates fibroblast contractile activity. . Mol. Biol. Cell , 12 : 2730 – 2741 .
   PubMed Web of Science ®Google Scholar
• Harrison , C. A. and MacNeil , S. 2008 . The mechanism of skin graft contraction: An update on current research and potential future therapies. . Burns , 34 : 153 – 163 .
   PubMed Web of Science ®Google Scholar
• Desai , M. H. , Mlakar , J. M. , McCauley , R. L. , Abdullah , K. M. , Rutan , R. L. and Waymack , J. P. 1991 . Lack of long term durability of cultured keratinocyte burn wound coverage: A case report. . J. Burn Care Rehabil. , 12 : 540 – 545 .
   Google Scholar
• Hansbrough , J. F. , Dore , C. and Hansbrough , W. B. 1992 . Clinical trials of a living dermal tissue replacement placed beneath meshed, split‐thickness skin grafts on excised wounds. . J. Burn Care Rehabil. , 13 : 519 – 529 .
   PubMedGoogle Scholar
• Parenteau , N. L. , Bilbo , P. , Nolte , C. J. , Mason , V. S. and Rosenberg , M. 1992 . The organotypic culture of human skin keratinocytes and fibroblasts to achieve form and function. . Cytotechnology , 9 : 163 – 171 .
   PubMed Web of Science ®Google Scholar
• Bannasch , H. , Unterberg , T. , Föhn , M. , Weyand , B. , Horch , R. E. and Stark , G. B. 2008 . Cultured keratinocytes in fibrin with decellularised dermis close porcine full‐thickness wounds in a single step. . Burns , 34 : 1015 – 1021 .
   PubMedGoogle Scholar