851
Views
50
CrossRef citations to date
0
Altmetric
Original

Connexin43 Expression Levels Influence Intercellular Coupling and Cell Proliferation of Native Murine Cardiac Fibroblasts

, , , , , & , PhD show all
Pages 289-303 | Received 16 Aug 2007, Published online: 11 Jul 2009

References

  • Axelsen LN, Stahlhut M, Mohammed S, Larsen BD, Nielsen MS, Holstein-Rathlou N-H, Andersen S, Jensen ON, Hennan JK, Kjølbye AL. Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123). J Mol Cell Cardiol. 2006; 40: 790–798
  • Bukauskas FF, Kreuzberg MM, Rackauskas M, Bukauskiene A, Bennett MVL, Verselis VK, Willecke K. Properties of mouse connexin 30.2 and human connexin 31.9 hemichannels: Implications for atrioventricular conduction in the heart. Proc Natl Acad Sci U S A. 2006; 103: 9726–9731
  • Camelliti P, Borg T, Kohl P. Structural and functional characterisation of cardiac fibroblasts. Cardiovasc Res. 2005; 65: 40–51
  • Camelliti P, Devlin GP, Matthews KG, Kohl P, Green CR. Spatially and temporally distinct expression of fibroblast connexins after sheep ventricular infarction. Cardiovasc Res. 2004a; 62: 415–425
  • Camelliti P, Green CR, LeGrice I, Kohl P. Fibroblast network in rabbit sinoatrial node: Structural and functional identification of homogeneous and heterogeneous cell coupling. Circ Res. 2004b; 94: 828–835
  • Cleutjens JPM, Kandala JC, Guarda E, Guntaka RV, Weber KT. Regulation of collagen degradation in the rat myocardium after infarction. J Mol Cell Cardiol. 1994; 27: 1281–1292
  • Coppen SR, Severs NJ, Gourdie RG. Connexin 45 (α6) expression delineates an extended conduction system in the embryonic and mature rodent heart. Dev Genet. 1999; 24: 82–90
  • De Maziere AMGL, Van Ginneken ACG, Wilders R, Jongsma HJ, Bouman LN. Spatial and functional relationship between myocytes and fibroblasts in the rabbit sinoatrial node. J Mol Cell Cardiol. 1992; 24: 567–578
  • Doble BW, Dang X, Ping P, Fandrich RR, Nickel BE, Jin Y, Cattini PA, Kardami E. Phosphorylation of serine 262 in the gap junction protein connexin–43 regulates DNA synthesis in cell-cell contact forming cardiomyocytes. J Cell Sci. 2004; 117: 507–514
  • Doble BW, Kardami E. Basic fibroblast growth factor stimulates connexin-43 expression and intercellular communication of cardiac fibroblasts. Mol Cell Biochem. 1995; 143: 81–87
  • Eghbali M, Blumenfeld OO, Seifter S, Buttrick PM, Leinwand LA, Robinson TF, Zern MA, Giambrone MA. Localization to types I, III and IV collagen mRNAs in rat heart cells by in situ hybridization. J Mol Cell Cardiol. 1989; 21: 103–113
  • El-Fouly MH, Trosko JE, Chang C-C. Scrape-loading and dye transfer: A rapid and simple technique to study gap junctional intercellular communication. Exp Cell Res. 1987; 168: 422–430
  • Gaudesius G, Miragoli M, Thomas SP, Rohr S. Coupling of cardiac electrical activity over extended distances by fibroblasts of cardiac origin. Circ Res. 2003; 93: 421–428
  • Goldsmith EC, Hoffman A, Morales MO, Potts JD, Price RL, McFadden A, Rice M, Borg TK. Organization of fibroblasts in the heart. Dev Dyn. 2004; 230: 787–794
  • Guo W, Xu H, London B, Nerbonne JM. Molecular basis of transient outward K+ current diversity in mouse ventricular myocytes. J Physiol. 1999; 521: 587–599
  • Hertlein B, Butterweck A, Haubrich S, Willecke K, Traub O. Phosphorylated carboxy terminal serine residues stabilize the mouse gap junction protein connexin45 against degradation. J Membr Biol. 1998; 162: 247–257
  • Hoppe UC, Marbán E, Johns DC. Adenovirus-mediated inducible gene expression in vivo by a hybrid ecdysone receptor. Mol Ther. 2000; 1: 159–164
  • Johnson CM, Green KG, Kanter EM, Bou-Abboud E, Saffitz JE, Yamada KA. Voltage-gated Na+ channel activity and connexin expression in Cx43-deficient myocytes. J Cardiovasc Electrophysiol. 1999; 10: 1390–1401
  • Kanno S, Saffitz JE. The role of myocardial gap junctions in electrical conduction and arrhythmogenesis. Cardiovasc Pathol. 2001; 10: 169–177
  • Koval M, Geist ST, Westphale EM, Kemendy EM, Civitelli R, Beyer EC, Steinberg TH. Transfected connexin45 alters gap junction permeability in cells expressing endogenous connexin43. J Cell Biol. 1995; 130: 987–995
  • Laing JG, Westphale EM, Engelmann GL, Beyer EC. Characterization of the gap junction protein, connexin45. J Membr Biol. 1994; 139: 31–40
  • Lampe PD, Cooper CD, King TJ, Burt JM. Analysis of connexin43 phosphorylated at S325, S328 and S330 in normoxic and ischemic heart. J Cell Sci. 2006; 119: 3435–3442
  • Lampe PD, Lau AF. The effects of connexin phosphorylation on gap junctional communication. Int J Biochem Cell Biol. 2004; 36: 1171–1186
  • Meyer RA, Laird DW, Revel J-P, Johnson RG. Inhibition of gap junction and adherens junction assembly by connexin and A-CAM antibodies. J Cell Biol. 1992; 119: 179–189
  • Miragoli M, Gaudesius G, Rohr S. Electrotonic modulation of cardiac impulse conduction by myofibroblasts. Circ Res. 2006; 98: 801–810
  • Musil LS, Cunningham BA, Edelman GM, Goodenough DA. Differential phosphorylation of the gap junction protein connexin43 in junctional communication-competent and -deficient cell lines. J Cell Biol. 1990; 111: 2077–2088
  • Musil LS, Le A-CN, VanSlyke JK, Roberts LM. Regulation of connexin degradation as a mechanism to increase gap junction assembly and function. J Biol Chem. 2000; 275: 25207–25215
  • Naus CCG. Gap junctions and tumour progression. Can J Physiol Pharmacol. 2002; 80: 136–141
  • Pepper MS, Meda P. Basic fibroblast growth factor increases junctional communication and connexin 43 expression in microvascular endothelial cells. J Cell Physiol. 1992; 153: 196–205
  • Pimentel RC, Yamada KA, Kléber AG, Saffitz JE. Autocrine regulation of myocyte Cx43 expression by VEGF. Circ Res. 2002; 90: 671–677
  • Shaw RM, Fay AJ, Puthenveedu MA, von Zastrow M, Jan Y-N, Jan LY. Microtubule plus-end-tracking proteins target gap junctions directly from the cell interior to adherens junctions. Cell. 2007; 128: 547–560
  • Silzle T, Randolph GJ, Kreutz M, Kunz-Schughart. The fibroblast: Sentinel cell and local immune modulator in tumor tissue. Int J Cancer. 2004; 108: 173–180
  • Smith RS, Smith TJ, Blieden TM, Phipps RP. Fibroblasts as sentinel cells: Synthesis of chemokines and regulation of inflammation. Am J Pathol. 1997; 151: 317–322
  • Solan JL, Fry MD, TenBroek EM, Lampe PD. Connexin43 phosphorylation at S368 is acute during S and G2/M and in response to protein kinase C activation. J Cell Sci. 2003; 116: 2203–2211
  • Sun Y, Kiani MF, Postlethwaite AE, Weber KT. Infarct scar as living tissue. Basic Res Cardiol. 2002; 97: 343–347
  • Sundset R, Cooper M, Mikalsen SO, Ytrehus K. Ischemic preconditioning protects against gap junctional uncoupling in cardiac myofibroblasts. Cell Commun Adhes. 2004; 11: 51–66
  • Veenstra RD. Size and selectivity of gap junction channels formed from different connexins. J Bioenerg Biomembr. 1996; 28: 327–337
  • Wang F, Trial J, Diwan A, Gao F, Birdsall H, Entman M, Hornsby P, Sivasubramaniam N, Mann DL. Regulation of cardiac fibroblast cellular function by leukemia inhibitory factor. J Mol Cell Cardiol. 2002; 34: 1309–1316
  • Xu H, Guo W, Nerbonne JM. Four kinetically distinct depolarization-activated K+ currents in adult mouse ventricular myocytes. J Gen Physiol. 1999; 113: 661–678
  • Yamada KA, Rogers JG, Sundset R, Steinberg TH, Saffitz JE. Up-regulation of connexin45 in heart failure. J Cardiovasc Electrophysiol. 2003; 14: 1205–1212
  • Zhang Y-W, Kaneda M, Morita I. The gap junction-independent tumor-suppressing effect of connexin 43. J Biol Chem. 2003a; 278: 44852–44856
  • Zhang Y-W, Nakayama K, Nakayama K-I, Morita I. A novel route for connexin 43 to inhibit cell proliferation: Negative regulation of S-phase kinase-associated protein (Skp 2). Cancer Res. 2003b; 63: 1623–1630
  • Zhuang J, Yamada KA, Saffitz JE, Kléber AG. Pulsatile stretch remodels cell-to-cell communication in cultured myocytes. Circ Res. 2000; 87: 316–322

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.