Transfection of hard-to-transfect primary human macrophages with Bax siRNA to reverse Resveratrol-induced apoptosis

References

• Wijburg OLC, van den Dobbelsteen GPJM, Vadolas J, et al. The role of macrophages in the induction and regulation of immunity elicited by exogenous antigens. Eur J Immunol. 1998;28:479–487.
   PubMed Web of Science ®Google Scholar
• Gordon S. The role of the macrophage in immune regulation. Research in Immunology. 1998;149:685–688.
   PubMedGoogle Scholar
• Park S-Y, Jung M-Y, Lee S-J, et al. Stabilin-1 mediates phosphatidylserine-dependent clearance of cell corpses in alternatively activated macrophages. Journal of Cell Science. 2009;122:3365–3373.
   PubMed Web of Science ®Google Scholar
• Wynn TA, Vannella KM. Macrophages in tissue repair, regeneration, and fibrosis. Immunity. 2016;44:450–462.
   PubMed Web of Science ®Google Scholar
• Perelson AS, Neumann AU, Markowitz M, et al. HIV-1 dynamics in vivo: virion clearance rate, infected cell life-span, and viral generation time. Science. 1996;271:1582–1586.
   PubMed Web of Science ®Google Scholar
• Crowe S, Zhu T, Muller WA. The contribution of monocyte infection and trafficking to viral persistence, and maintenance of the viral reservoir in HIV infection. Journal of Leukocyte Biology. 2003;74:635–641.
   PubMed Web of Science ®Google Scholar
• Chen Y, Zhang X. Pivotal regulators of tissue homeostasis and cancer: macrophages. Exp Hematol Oncol. 2017;6:23.
   PubMed Web of Science ®Google Scholar
• Krysko O, Holtappels G, Zhang N, et al. Alternatively activated macrophages and impaired phagocytosis of S aureus in chronic rhinosinusitis. Allergy. 2011;66:396–403.
   PubMed Web of Science ®Google Scholar
• Firszt R, Vickery B. An interferon-inducible neutrophil-driven blood transcriptional signature in human tuberculosis. Pediatrics. 2011;128:S145–S146.
   Google Scholar
• Montoya D, Cruz D, Teles RMB, et al. Divergence of macrophage phagocytic and antimicrobial programs in leprosy. Cell Host Microbe. 2009;6:343–353.
   PubMed Web of Science ®Google Scholar
• Quiding-Järbrink M, Raghavan S, Sundquist M. Enhanced M1 macrophage polarization in human helicobacter pylori-associated atrophic gastritis and in vaccinated mice. PLoS One. 2010;5:e15018.
   PubMed Web of Science ®Google Scholar
• Mège J-L, Mehraj V, Capo C. Macrophage polarization and bacterial infections. Current Opinion in Infectious Diseases. 2011;24:230–234.
   PubMed Web of Science ®Google Scholar
• Thompson LJ, Dunstan SJ, Dolecek C, et al. Transcriptional response in the peripheral blood of patients infected with Salmonella enterica serovar Typhi. Proceedings of the National Academy of Sciences. 2009;106:22433–22438.
   PubMed Web of Science ®Google Scholar
• Parisi L, Gini E, Baci D, et al. Macrophage polarization in chronic inflammatory diseases: killers or builders? Journal of Immunology Research. 2018;2018:1–25.
   Web of Science ®Google Scholar
• Shapouri-Moghaddam A, Mohammadian S, Vazini H, et al. Macrophage plasticity, polarization, and function in health and disease. J Cell Physiol. 2018;233:6425–6440.
   PubMed Web of Science ®Google Scholar
• Ponzoni M, Pastorino F, Di Paolo D, et al. Targeting macrophages as a potential therapeutic intervention: impact on inflammatory diseases and cancer. International Journal of Molecular Sciences. 2018;19:1953.
   Web of Science ®Google Scholar
• Hadji P, Coleman R, Gnant M, et al. The impact of menopause on bone, zoledronic acid, and implications for breast cancer growth and metastasis. Annals of Oncology. 2012;23:2782–2790.
   PubMed Web of Science ®Google Scholar
• Dahl V, Josefsson L, Palmer S. HIV reservoirs, latency, and reactivation: prospects for eradication. Antiviral Research. 2010;85:286–294.
   PubMed Web of Science ®Google Scholar
• Sica A, Mantovani A. Macrophage plasticity and polarization: in vivo veritas. J Clin Invest. 2012;122:787–795.
   PubMed Web of Science ®Google Scholar
• Koren-Gluzer M, Rosenblat M, Hayek T. Paraoxonase 2 induces a phenotypic switch in macrophage polarization favoring an M2 anti-inflammatory state. International Journal of Endocrinology. 2015;2015:1–9.
   Web of Science ®Google Scholar
• Porcheray F, Viaud S, Rimaniol A-C, et al. Macrophage activation switching: an asset for the resolution of inflammation. Clin Exp Immunol. 2005;142:481–489.
   PubMed Web of Science ®Google Scholar
• Weiss WA, Taylor SS, Shokat KM. Recognizing and exploiting differences between RNAi and small-molecule inhibitors. Nat Chem Biol. 2007;3:739–744.
   PubMed Web of Science ®Google Scholar
• Ovcharenko D, Kelnar K, Johnson C, et al. Genome-scale MicroRNA and small interfering RNA screens identify Small RNA modulators of TRAIL-induced apoptosis pathway. Cancer Res. 2007;67:10782–10788.
   PubMed Web of Science ®Google Scholar
• Xia Y, Gates B, Yin Y. Building complex structures from monodisperse spherical colloids. Aust J Chem. 2001;54:287–290.
   Web of Science ®Google Scholar
• Stroh T, Erben U, Kühl AA, et al. Combined pulse electroporation – a novel strategy for highly efficient transfection of human and mouse cells. PLoS One. 2010;5:e9488.
   PubMed Web of Science ®Google Scholar
• Dokka S, Toledo D, Shi X, et al. High-efficiency gene transfection of macrophages by lipoplexes. International Journal of Pharmaceutics. 2000;206:97–104.
   PubMed Web of Science ®Google Scholar
• Keller -A-A, Maeß MB, Schnoor M, et al. Transfecting macrophages. Methods in Mol Biol. 2018;1784:187–195.
   Google Scholar
• Koppensteiner H, Brack-Werner R, Schindler M. Macrophages and their relevance in human immunodeficiency virus type I infection. Retrovirology. 2012;9:82.
   PubMed Web of Science ®Google Scholar
• DiNapoli SR, Hirsch VM, Brenchley JM. Macrophages in progressive human immunodeficiency virus/simian immunodeficiency virus infections. J Virol. 2016;90:7596–7606.
   PubMed Web of Science ®Google Scholar
• Clayton KL, Garcia V, Clements JE, et al. HIV infection of macrophages: implications for pathogenesis and cure. Pathogens and Immunity. 2017;2:179.
   PubMedGoogle Scholar
• Aquaro S, Caliò R, Balzarini J, et al. Macrophages and HIV infection: therapeutical approaches toward this strategic virus reservoir. Antiviral Research. 2002;55:209–225.
   PubMed Web of Science ®Google Scholar
• Spagnou S, Miller AD, Keller M. Lipidic carriers of siRNA: differences in the formulation, cellular uptake, and delivery with plasmid DNA. Biochemistry. 2004;43:13348–13356.
   PubMed Web of Science ®Google Scholar
• Breunig M, Lungwitz U, Liebl R, et al. Breaking up the correlation between efficacy and toxicity for nonviral gene delivery. Proceedings of the National Academy of Sciences. 2007;104:14454–14459.
   PubMed Web of Science ®Google Scholar
• Reynolds A. Induction of the interferon response by siRNA is cell type- and duplex length-dependent. RNA. 2006;12:988–993.
   PubMed Web of Science ®Google Scholar
• Guha P, Dey A, Sen R, et al. Intracellular GSH depletion triggered mitochondrial bax translocation to accomplish Resveratrol-induced apoptosis in the U937 cell line. J Pharmacol Exp Ther. 2011;336:206–214.
   PubMed Web of Science ®Google Scholar
• Single A, Beetham H, Telford BJ, et al. A comparison of real-time and endpoint cell viability assays for improved synthetic lethal drug validation. J Biomol Screen. 2015;20:1286–1293.
   PubMedGoogle Scholar
• Li Y, Yuan J. Systematic metrics depicting cell death kinetics. Cell Chem Biol. 2017;24:785–786.
   PubMed Web of Science ®Google Scholar
• Busca A, Saxena M, Kumar A. Critical role for antiapoptotic Bcl-xL and Mcl-1 in human macrophage survival and cellular IAP1/2 (cIAP1/2) in resistance to HIV-Vpr-induced apoptosis. J Biol Chem. 2012;287:15118–15133.
   PubMed Web of Science ®Google Scholar
• Liu L, Oliveira NMM, Cheney KM, et al. A whole genome screen for HIV restriction factors. Retrovirology. 2011;8:94.
   PubMed Web of Science ®Google Scholar
• Chemudupati M, Kenney AD, Bonifati S, et al. From APOBEC to ZAP: diverse mechanisms used by cellular restriction factors to inhibit virus infections. Biochimica Et Biophysica Acta (BBA) - Molecular Cell Research. 2019;1866:382–394.
   PubMed Web of Science ®Google Scholar
• Ryoo J, Choi J, Oh C, et al. The ribonuclease activity of SAMHD1 is required for HIV-1 restriction. Nat Med. 2014;20:936–941.
   PubMed Web of Science ®Google Scholar
• Tietz SM, Berghoff M. Gene silencing of MK2 in hard-to-transfect human U937 cells. J Biomol Tech. 2012;23:47–50.
   PubMedGoogle Scholar
• Mehrotra S, Lee I, Chan C. Multilayer mediated forward and patterned siRNA transfection using linear-PEI at extended N/P ratios. Acta Biomater. 2009;5:1474–1488.
   PubMed Web of Science ®Google Scholar
• Iorns E, Lord CJ, Ashworth A. Parallel RNAi and compound screens identify the PDK1 pathway as a target for tamoxifen sensitization. Biochemical Journal. 2009;417:361–371.
   PubMed Web of Science ®Google Scholar
• Pink RC, Samuel P, Massa D, et al. The passenger strand, miR-21-3p, plays a role in mediating cisplatin resistance in ovarian cancer cells. Gynecologic Oncology. 2015;137:143–151.
   PubMed Web of Science ®Google Scholar
• Tsofack SP, Garand C, Sereduk C, et al. NONO and RALY proteins are required for YB-1 oxaliplatin induced resistance in colon adenocarcinoma cell lines. Mol Cancer. 2011;10:145.
   PubMed Web of Science ®Google Scholar