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RNA Biology Volume 18, 2021 - Issue 11
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Brief Communication

Ribosome profiling analysis of human skeletal muscle identifies reduced translation of mitochondrial proteins with age

Ravi Tharakana Translational Gerontology Branch, National Institutes of Health, Baltimore, MD, USA;b Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, MD, USAView further author information
,
Ceereena Ubaida-Mohiena Translational Gerontology Branch, National Institutes of Health, Baltimore, MD, USAView further author information
,
Yulan Piaob Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, MD, USAView further author information
,
Myriam Gorospeb Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, MD, USAView further author information
&
Luigi Ferruccia Translational Gerontology Branch, National Institutes of Health, Baltimore, MD, USACorrespondence[email protected]
View further author information
Pages 1555-1559 | Received 15 Aug 2020, Accepted 09 Jan 2021, Published online: 21 Jan 2021

References

  • Kaeberlein M, Kennedy BK. Ageing: A midlife longevity drug? Nature. 2009;460(7253):331–332.
  • Johnson SC, Rabinovitch PS, Kaeberlein M. mTOR is a key modulator of ageing and age-related disease. Nature. 2013;493(7432):338–345.
  • Hansen M, Taubert S, Crawford D, et al. Lifespan extension by conditions that inhibit translation in Caenorhabditis elegans. Aging Cell. 2007;6(1):95–110.
  • Hamilton B, Dong Y, Shindo M, et al. A systematic RNAi screen for longevity genes in C. elegans. Genes Dev. 2005;19(13):1544–1555.
  • Syntichaki P, Troulinaki K, Tavernarakis N. eIF4E function in somatic cells modulates ageing in Caenorhabditis elegans. Nature. 2007;445(7130):922–926.
  • Solis GM, Kardakaris R, Valentine ER, et al. Translation attenuation by minocycline enhances longevity and proteostasis in old post-stress-responsive organisms. Elife. 2018;7. DOI:10.7554/eLife.40314.
  • Demontis F, Perrimon N. FOXO/4E-BP signaling in Drosophila muscles regulates organism-wide proteostasis during aging. Cell. 2010;143(5):813–825.
  • Welle S, Thornton C, Jozefowicz R, et al. Myofibrillar protein synthesis in young and old men. Am J Physiol. 1993;264(5 Pt 1):E693–698.
  • Taanman JW. The mitochondrial genome: structure, transcription, translation and replication. Biochim Biophys Acta. 1999;1410(2):103–123.
  • Weingarten-Gabbay S, Elias-Kirma S, Nir R, et al. Comparative genetics. Systematic discovery of cap-independent translation sequences in human and viral genomes. Science. 2016;351(6270):6270.
  • Asnani M, Pestova TV, Hellen CU. PCBP2 enables the cadicivirus IRES to exploit the function of a conserved GRNA tetraloop to enhance ribosomal initiation complex formation. Nucleic Acids Res. 2016;44(20):9902–9917.
  • Ubaida-Mohien C, Lyashkov A, Gonzalez-Freire M, et al. Discovery proteomics in aging human skeletal muscle finds change in spliceosome, immunity, proteostasis and mitochondria. Elife. 2019;8. DOI:10.7554/eLife.49874.
  • Peterson CM, Johannsen DL, Ravussin E. Skeletal muscle mitochondria and aging: a review. J Aging Res. 2012;2012:194821.
  • Marcus DL, Ibrahim NG, Freedman ML. Age-related decline in the biosynthesis of mitochondrial inner membrane proteins. Exp Gerontol. 1982;17(5):333–341.
  • Rooyackers OE, Adey DB, Ades PA, et al. Effect of age on in vivo rates of mitochondrial protein synthesis in human skeletal muscle. Proc Natl Acad Sci U S A. 1996;93(26):15364–15369.
  • Miller BF, Robinson MM, Bruss MD, et al. A comprehensive assessment of mitochondrial protein synthesis and cellular proliferation with age and caloric restriction. Aging Cell. 2012;11(1):150–161.
  • Temperley RJ, Wydro M, Lightowlers RN, et al. Human mitochondrial mRNAs–like members of all families, similar but different. Biochim Biophys Acta. 2010;1797(6–7):1081–1085.
  • Weraarpachai W, Antonicka H, Sasarman F, et al. Mutation in TACO1, encoding a translational activator of COX I, results in cytochrome c oxidase deficiency and late-onset Leigh syndrome. Nat Genet. 2009;41(7):833–837.
  • Gonzalez-Freire M, Scalzo P, D’Agostino J, et al. Skeletal muscle ex vivo mitochondrial respiration parallels decline in vivo oxidative capacity, cardiorespiratory fitness, and muscle strength: the Baltimore Longitudinal Study of Aging. Aging Cell. 2018;17(2):e12725.
  • Ingolia NT, Brar GA, Rouskin S, et al. The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. Nat Protoc. 2010;1797(6–7):1534–1550.
  • McGlincy NJ, Ingolia NT. Transcriptome-wide measurement of translation by ribosome profiling. Methods. 2017;126:112–129.
  • Patro R, Duggal G, Love MI, et al. Salmon provides fast and bias-aware quantification of transcript expression. Nat Methods. 2017;14(4):417–419.
  • Li W, Wang W, Uren PJ, et al. Riborex: fast and flexible identification of differential translation from Ribo-seq data. Bioinformatics. 2017;33(11):1735–1737.
  • Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014;15(12):550.
  • Ravi Tharakan CU-M, Piao Y, Gorospe M, et al. Ribosome profiling analysis of aging human skeletal muscle. NCBI Gene Expression Omnibus. 2020.

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