Acute effects of various doses of nitrate-rich beetroot juice on high-intensity interval exercise responses in women: a randomized, double-blinded, placebo-controlled, crossover trial

References

• Moncada, S, Higgs, A. The L-Arginine-nitric oxide pathway. N Engl J Med. 1993;329(27):2002–240. doi: 10.1056/NEJM199312303292706
   PubMed Web of Science ®Google Scholar
• Brown, GC, Cooper, CE. Nanomolar concentrations of nitric oxide reversibly inhibit synaptosomal respiration by competing with oxygen at cytochrome oxidase. FEBS Lett. 1994;356(2–3):295–298. doi: 10.1016/0014-5793(94)01290-3
   PubMed Web of Science ®Google Scholar
• Stamler, JS, Meissner, G. Physiology of nitric oxide in skeletal muscle. Physiol Rev. 2001;81(1):209–237. doi: 10.1152/physrev.2001.81.1.209
   PubMed Web of Science ®Google Scholar
• Percival, JM, Anderson, KNE, Huang, P, et al. Golgi and sarcolemmal neuronal NOS differentially regulate contraction-induced fatigue and vasoconstriction in exercising mouse skeletal muscle. J Clin Invest. 2010;120(3):816–826. doi: 10.1172/JCI40736
   PubMed Web of Science ®Google Scholar
• Benjamin, N, O’Driscoll, F, Dougall, H, et al. Stomach NO synthesis. Nature. 1994;368(6471):502–502. doi: 10.1038/368502a0
   PubMed Web of Science ®Google Scholar
• Bredt, DS. Endogenous nitric oxide synthesis: biological functions and pathophysiology. Free Radic Res. 1999;31(6):577–596. doi: 10.1080/10715769900301161
   PubMed Web of Science ®Google Scholar
• Lundberg, JO, Weitzberg, E, Lundberg, JM, et al. Intragastric nitric oxide production in humans: measurements in expelled air. Gut. 1994;35(11):1543. doi: 10.1136/gut.35.11.1543
   PubMed Web of Science ®Google Scholar
• Hord, NG, Tang, Y, Bryan, NS. Food sources of nitrates and nitrites: the physiologic context for potential health benefits2. Am J Clin Nutr. 2009;90(1):1–10. doi: 10.3945/ajcn.2008.27131
   PubMed Web of Science ®Google Scholar
• Ysart, G, Miller, P, Barrett, G, et al. Dietary exposures to nitrate in the UK. Food Addit Contam. 1999;16(12):521–532. doi: 10.1080/026520399283669
   PubMed Web of Science ®Google Scholar
• Bailey, SJ, Fulford, J, Vanhatalo, A, et al. Dietary nitrate supplementation enhances muscle contractile efficiency during knee-extensor exercise in humans. J Appl Physiol. 2010;109(1):135–148. doi: 10.1152/japplphysiol.00046.2010
   PubMed Web of Science ®Google Scholar
• Whitfield, J, Gamu, D, Heigenhauser, GJF, et al. Beetroot juice increases human muscle force without changing Ca2+-Handling proteins. Med Sci Sports Exerc. 2017;49(10):2016–2024. doi: 10.1249/MSS.0000000000001321
   PubMed Web of Science ®Google Scholar
• Hernández, A, Schiffer, TA, Ivarsson, N, et al. Dietary nitrate increases tetanic [Ca2+]i and contractile force in mouse fast-twitch muscle. J Physiol. 2012;590(15):3575–3583. doi: 10.1113/jphysiol.2012.232777
   PubMed Web of Science ®Google Scholar
• Evangelista, AM, Rao, VS, Filo, AR, et al. Direct regulation of striated muscle myosins by nitric oxide and endogenous nitrosothiols. PLoS One. 2010;5(6):e11209. doi: 10.1371/journal.pone.0011209
   PubMed Web of Science ®Google Scholar
• Nogueira, L, Figueiredo-Freitas, C, Casimiro-Lopes, G, et al. Myosin is reversibly inhibited by S-nitrosylation. Biochem J. 2009;424(2):221–231. doi: 10.1042/BJ20091144
   PubMedGoogle Scholar
• Maughan, RJ, Burke, LM, Dvorak, J, et al. IOC consensus statement: dietary supplements and the high-performance athlete. Int J Sport Nutr Exercise Metab. 2018;28(2):104–125. doi: 10.1123/ijsnem.2018-0020
   PubMed Web of Science ®Google Scholar
• Bailey, SJ, Varnham, RL, DiMenna, FJ, et al. Inorganic nitrate supplementation improves muscle oxygenation, O₂ uptake kinetics, and exercise tolerance at high but not low pedal rates. J Appl Physiol. 2015;118(11):1396–1405. doi: 10.1152/japplphysiol.01141.2014
   PubMed Web of Science ®Google Scholar
• McMahon, NF, Leveritt, MD, Pavey, TG. The effect of dietary nitrate supplementation on endurance exercise performance in healthy adults: a systematic review and meta-analysis. Sports Med. 2017;47(4):735–756. doi: 10.1007/s40279-016-0617-7
   PubMed Web of Science ®Google Scholar
• Murphy, M, Eliot, K, Heuertz, RM, et al. Whole beetroot consumption acutely improves running performance. J Acad Nutr Diet. 2012;112(4):548–552. doi: 10.1016/j.jand.2011.12.002
   PubMed Web of Science ®Google Scholar
• Boorsma, RK, Whitfield, J, Spriet, LL. Beetroot juice supplementation does not improve performance of elite 1500-m runners. Med Sci Sports Exerc. 2014;46(12):2326–2334. doi: 10.1249/MSS.0000000000000364
   PubMed Web of Science ®Google Scholar
• Fernandes de Castro, T, de Assis Manoel, F, Machado, FA. Beetroot juice supplementation does not modify the 3-km running performance in untrained women. Sci Sports. 2018;33(4):e167–e170. doi: 10.1016/j.scispo.2018.01.010
   Google Scholar
• Bailey, SJ, Winyard, P, Vanhatalo, A, et al. Dietary nitrate supplementation reduces the O2 cost of low-intensity exercise and enhances tolerance to high-intensity exercise in humans. J Appl Physiol. 2009;107(4):1144–1155. doi: 10.1152/japplphysiol.00722.2009
   PubMed Web of Science ®Google Scholar
• Cermak, NM, Gibala, MJ, van Loon, LJ. Nitrate supplementation’s improvement of 10-km time-trial performance in trained cyclists. Int J Sport Nutr Exerc Metab. 2012;22(1):64–71. doi: 10.1123/ijsnem.22.1.64
   PubMed Web of Science ®Google Scholar
• Breese, BC, McNarry, MA, Marwood, S, et al. Beetroot juice supplementation speeds O2 uptake kinetics and improves exercise tolerance during severe-intensity exercise initiated from an elevated metabolic rate. Am J Physiol Regul Integr Comp Physiol. 2013;305(12):R1441–R1450. doi: 10.1152/ajpregu.00295.2013
   PubMed Web of Science ®Google Scholar
• Larsen, FJ, Schiffer, TA, Borniquel, S, et al. Dietary inorganic nitrate improves mitochondrial efficiency in humans. Cell Metab. 2011;13(2):149–159. doi: 10.1016/j.cmet.2011.01.004
   PubMed Web of Science ®Google Scholar
• Muggeridge, DJ, Howe, CC, Spendiff, O, et al. A single dose of beetroot juice enhances cycling performance in simulated altitude. Med Sci Sports Exerc. 2014;46(1):143–150. doi: 10.1249/MSS.0b013e3182a1dc51
   PubMed Web of Science ®Google Scholar
• Wylie, LJ, Park, JW, Vanhatalo, A, et al. Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise. J Physiol. 2019;597(23):5565–5576. doi: 10.1113/JP278076
   PubMed Web of Science ®Google Scholar
• Larsen, FJ, Weitzberg, E, Lundberg, JO, et al. Dietary nitrate reduces maximal oxygen consumption while maintaining work performance in maximal exercise. Free Radic Biol Med. 2010;48(2):342–347. doi: 10.1016/j.freeradbiomed.2009.11.006
   PubMed Web of Science ®Google Scholar
• Muggeridge, DJ, Howe, CC, Spendiff, O, et al. The effects of a single dose of concentrated beetroot juice on performance in trained flatwater kayakers. Int J Sport Nutr Exerc Metab. 2013;23(5):498–506. doi: 10.1123/ijsnem.23.5.498
   PubMed Web of Science ®Google Scholar
• Hoon, MW, Jones, AM, Johnson, NA, et al. The effect of variable doses of inorganic nitrate-rich beetroot juice on simulated 2,000-m rowing performance in trained athletes. Int J Sports Physiol Perform. 2014;9(4):615–620. doi: 10.1123/ijspp.2013-0207
   PubMed Web of Science ®Google Scholar
• Wylie, LJ, Bailey, SJ, Kelly, J, et al. Influence of beetroot juice supplementation on intermittent exercise performance. Eur J Appl Physiol. 2016;116(2):415–425. doi: 10.1007/s00421-015-3296-4
   PubMed Web of Science ®Google Scholar
• Thompson, C, Wylie, LJ, Fulford, J, et al. Dietary nitrate improves sprint performance and cognitive function during prolonged intermittent exercise. Eur J Appl Physiol. 2015;115(9):1825–1834. doi: 10.1007/s00421-015-3166-0
   PubMed Web of Science ®Google Scholar
• Pawlak-Chaouch, M, Boissière, J, Munyaneza, D, et al. Beetroot juice does not enhance supramaximal intermittent exercise performance in elite endurance athletes. J Am Coll Nutr. 2019;38(8):729–738. doi: 10.1080/07315724.2019.1601601
   PubMed Web of Science ®Google Scholar
• Broeder, CE, Flores, V, Julian, B, et al. Nitric oxide enhancement supplement containing beet nitrite and nitrate benefits high intensity cycle interval training. Curr Res Physiol. 2021;4:183–191. doi: 10.1016/j.crphys.2021.07.002
   PubMedGoogle Scholar
• Bender, D, Townsend, JR, Vantrease, WC, et al. Acute beetroot juice administration improves peak isometric force production in adolescent males. Appl Physiol Nutr Metab. 2018;43(8):816–821. doi: 10.1139/apnm-2018-0050
   PubMed Web of Science ®Google Scholar
• Aucouturier, J, Boissière, J, Pawlak-Chaouch, M, et al. Effect of dietary nitrate supplementation on tolerance to supramaximal intensity intermittent exercise. Nitric Oxide. 2015;49:16–25. doi: 10.1016/j.niox.2015.05.004
   PubMed Web of Science ®Google Scholar
• Coates, AM, Joyner, MJ, Little, JP, et al. A perspective on high-intensity interval training for performance and health. Sports Med. 2023;53(Suppl 1):85–96. doi: 10.1007/s40279-023-01938-6
   PubMed Web of Science ®Google Scholar
• Thompson, WR. Worldwide survey of fitness trends for 2023. ACSMs Health Fit J. 2023;27(1):9–18. doi: 10.1249/FIT.0000000000000834
   Web of Science ®Google Scholar
• Esen, O, Domínguez, R, Karayigit, R. Acute beetroot juice supplementation enhances intermittent running performance but does not reduce oxygen cost of exercise among recreational adults. Nutrients. 2022;14(14):2839. doi: 10.3390/nu14142839
   PubMed Web of Science ®Google Scholar
• Nyakayiru, J, Jonvik, KL, Trommelen, J, et al. Beetroot juice supplementation improves high-intensity intermittent type exercise performance in trained soccer players. Nutrients. 2017;9(3):314. doi: 10.3390/nu9030314
   PubMed Web of Science ®Google Scholar
• Thompson, C, Vanhatalo, A, Jell, H, et al. Dietary nitrate supplementation improves sprint and high-intensity intermittent running performance. Nitric Oxide. 2016;61:55–61. doi: 10.1016/j.niox.2016.10.006
   PubMed Web of Science ®Google Scholar
• Silva, KVC, Costa, BD, Gomes, AC, et al. Factors that moderate the effect of nitrate ingestion on exercise performance in adults: a systematic review with meta-analyses and meta-regressions. Adv Nutr. 2022;13(5):1866–1881. doi: 10.1093/advances/nmac054
   PubMed Web of Science ®Google Scholar
• Wickham, KA, Spriet, LL. No longer beeting around the bush: a review of potential sex differences with dietary nitrate supplementation. Appl Physiol Nutr Metab. 2019;44(9):915–924. doi: 10.1139/apnm-2019-0063
   PubMed Web of Science ®Google Scholar
• Brown, M. Skeletal muscle and bone: effect of sex steroids and aging. Adv Physiol Educ. 2008;32(2):120–126. doi: 10.1152/advan.90111.2008
   PubMed Web of Science ®Google Scholar
• Nyakayiru, J, Kouw, IWK, Cermak, NM, et al. Sodium nitrate ingestion increases skeletal muscle nitrate content in humans. J Appl Physiol. 2017;123(3):637–644. doi: 10.1152/japplphysiol.01036.2016
   PubMed Web of Science ®Google Scholar
• Reckelhoff, JF. Gender differences in the regulation of blood pressure. Hypertension. 2001;37(5):1199–1208. doi: 10.1161/01.HYP.37.5.1199
   PubMed Web of Science ®Google Scholar
• World Health Organization. Regional Office for the Western P. The Asia-Pacific perspective : redefining obesity and its treatment. Sydney: Health Communications Australia; 2000.
   Google Scholar
• Craig, CL, Marshall, AL, Sjöström, M, et al. International physical activity questionnaire: 12-country reliability and validity. Med Sci Sports Exerc. 2003;35(8):1381–1395. doi: 10.1249/01.MSS.0000078924.61453.FB
   PubMed Web of Science ®Google Scholar
• Baranauskas, MN, Altherr, CA, Gruber, AH, et al. Beetroot supplementation in women enjoying exercise together (BEE SWEET): rationale, design and methods. Contemp Clin Trials Commun. 2021;21:100693. doi: 10.1016/j.conctc.2020.100693
   PubMed Web of Science ®Google Scholar
• Poon, E-C, Little, JP, Sit, C-P, et al. The effect of low-volume high-intensity interval training on cardiometabolic health and psychological responses in overweight/obese middle-aged men. J Sports Sci. 2020;38(17):1997–2004. doi: 10.1080/02640414.2020.1766178
   PubMed Web of Science ®Google Scholar
• Wylie, LJ, Kelly, J, Bailey, SJ, et al. Beetroot juice and exercise: pharmacodynamic and dose-response relationships. J Appl Physiol. 2013;115(3):325–336. doi: 10.1152/japplphysiol.00372.2013
   PubMed Web of Science ®Google Scholar
• Lansley, KE, Winyard, PG, Bailey, SJ, et al. Acute dietary nitrate supplementation improves cycling time trial performance. Med Sci Sports Exerc. 2011;43(6):1125–1131. doi: 10.1249/MSS.0b013e31821597b4
   PubMed Web of Science ®Google Scholar
• Govoni, M, Jansson, EÅ, Weitzberg, E, et al. The increase in plasma nitrite after a dietary nitrate load is markedly attenuated by an antibacterial mouthwash. Nitric Oxide. 2008;19(4):333–337. doi: 10.1016/j.niox.2008.08.003
   PubMed Web of Science ®Google Scholar
• Gallardo, EJ, Coggan, AR. What is in your beet juice? Nitrate and nitrite content of beet juice products marketed to athletes. Int J Sport Nutr Exercise Metab. 2019;29(4):345–349. doi: 10.1123/ijsnem.2018-0223
   PubMed Web of Science ®Google Scholar
• Webb, AJ, Patel, N, Loukogeorgakis, S, et al. Acute blood pressure lowering, Vasoprotective, and Antiplatelet Properties of Dietary Nitrate via bioconversion to nitrite. Hypertension. 2008;51(3):784–790. doi: 10.1161/hypertensionaha.107.103523
   PubMed Web of Science ®Google Scholar
• Kilpatrick, MW, Jung, ME, Little, JP. High-Intensity Interval Training: a review of physiological and psychological responses. ACSMs Health Fit J. 2014;18(5):11–16. doi: 10.1249/FIT.0000000000000067
   Web of Science ®Google Scholar
• Borg, G. Interindividual scaling and perception of muscular force. Department of Psychiatry, Medical School; 1961.
   Google Scholar
• Bradley, MM, Lang, PJ. Measuring emotion: the self-assessment manikin and the semantic differential. J Behav Ther Exp Psychiatry. 1994;25(1):49–59. doi: 10.1016/0005-7916(94)90063-9
   PubMed Web of Science ®Google Scholar
• Cohen, J. Statistical power analysis for the behavioral sciences. L. Erlbaum Associates; 1988.
   Google Scholar
• Borlaug, BA, Koepp, KE, Melenovsky, V. Sodium nitrite improves exercise hemodynamics and ventricular performance in heart failure with preserved ejection fraction. J Am Coll Cardiol. 2015;66(15):1672–1682. doi: 10.1016/j.jacc.2015.07.067
   PubMed Web of Science ®Google Scholar
• Pironti, G, Ivarsson, N, Yang, J, et al. Dietary nitrate improves cardiac contractility via enhanced cellular Ca2+ signaling. Basic Res Cardiol. 2016;111(3):34. doi: 10.1007/s00395-016-0551-8
   PubMed Web of Science ®Google Scholar
• Thompson, C, Wylie, LJ, Blackwell, JR, et al. Influence of dietary nitrate supplementation on physiological and muscle metabolic adaptations to sprint interval training. J Appl Physiol. 2017;122(3):642–652. doi: 10.1152/japplphysiol.00909.2016
   PubMed Web of Science ®Google Scholar
• Castello, PR, David, PS, McClure, T, et al. Mitochondrial cytochrome oxidase produces nitric oxide under hypoxic conditions: implications for oxygen sensing and hypoxic signaling in eukaryotes. Cell Metab. 2006;3(4):277–287. doi: 10.1016/j.cmet.2006.02.011
   PubMed Web of Science ®Google Scholar
• Modin, A, Björne, H, Herulf, M, et al. Nitrite-derived nitric oxide: a possible mediator of ‘acidic–metabolic’ vasodilation. Acta Physiol Scand. 2001;171(1):9–16. doi: 10.1046/j.1365-201X.2001.00771.x
   PubMedGoogle Scholar
• Coggan, AR, Leibowitz, JL, Kadkhodayan, A, et al. Effect of acute dietary nitrate intake on maximal knee extensor speed and power in healthy men and women. Nitric Oxide. 2015;48:16–21. doi: 10.1016/j.niox.2014.08.014
   PubMed Web of Science ®Google Scholar
• Ekkekakis, P, Parfitt, G, Petruzzello, SJ. The pleasure and displeasure people feel when they exercise at different intensities: decennial update and progress towards a tripartite rationale for exercise intensity prescription. Sports Med. 2011;41(8):641–671. doi: 10.2165/11590680-000000000-00000
   PubMed Web of Science ®Google Scholar