Interpreting γ-hydroxybutyrate concentrations for clinical and forensic purposes

References

• Ciolino LA, Mesmer MZ, Satzger RD. The chemical interconversion of GHB and GBL: forensic issues and implications. J Forensic Sci. 2001;46:1315–1323.
   PubMed Web of Science ®Google Scholar
• Busardo FP, Jones AW. GHB pharmacology and toxicology: acute intoxication, concentrations in blood and urine in forensic cases and treatment of the withdrawal syndrome. Curr Neuropharmacol. 2015;13:47–70.
   PubMed Web of Science ®Google Scholar
• Vickers MD. Gamma hydroxybutyric acid. Clinical pharmacology and current status. Proc R Soc Med. 1968;61:821–824.
   PubMedGoogle Scholar
• Hoes MJ, Vree TB, Guelen PJ. Gamma-hydroxybutyric acid as hypnotic. Clinical and pharmacokinetic evaluation of gamma-hydroxybutyric acid as hypnotic in man. Encephale 1980;6:93–99.
   PubMedGoogle Scholar
• Helrich M, McAslan TC, Skolnik S. Correlation of blood levels of 4-hydroxybutyrate with state of consciousness. Anesthesiology 1964;25:771–775.
   PubMed Web of Science ®Google Scholar
• Takahara J, Yunoki S, Yakushiji W, et al. Stimulatory effects of gamma-hydroxybutyric acid on growth hormone and prolactin release in humans. J Clin Endocrinol Metab. 1977;44:1014–1017.
   PubMed Web of Science ®Google Scholar
• Brailsford AD, Bartlett C, Kicman AT, et al. Increases in serum growth hormone concentrations associated with GHB administration. J Anal Toxicol. 2017;41:54–59.
   PubMed Web of Science ®Google Scholar
• Wong CG, Chan KF, Gibson KM, et al. Gamma-hydroxybutyric acid: neurobiology and toxicology of a recreational drug. Toxicol Rev. 2004;23:3–20.
   PubMedGoogle Scholar
• Brunt TM, van Amsterdam JG, van den Brink W. GHB, GBL and 1,4-BD addiction. Curr Pharm Des. 2014;20:4076–4085.
   PubMed Web of Science ®Google Scholar
• Schep LJ, Knudsen K, Slaughter RJ, et al. The clinical toxicology of gamma-hydroxybutyrate, gamma-butyrolactone and 1,4-butanediol. Clin Toxicol. 2012;50:458–470.
   PubMed Web of Science ®Google Scholar
• Thai D, Dyer JE, Jacob P, et al. Clinical pharmacology of 1,4-butanediol and gamma-hydroxybutyrate after oral 1,4-butanediol administration to healthy volunteers. Clin Pharmacol Ther. 2007;81:178–184.
   PubMed Web of Science ®Google Scholar
• Palmer RB. Gamma-butyrolactone and 1,4-butanediol: abused analogues of gamma-hydroxybutyrate. Toxicol Rev. 2004;23:21–31.
   PubMedGoogle Scholar
• Lora-Tamayo C, Tena T, Rodriguez A, et al. Intoxication due to 1,4-butanediol. Forensic Sci Int. 2003;133:256–259.
   PubMed Web of Science ®Google Scholar
• Poldrugo F, Snead OC. 3rd,. 1,4 Butanediol, gamma-hydroxybutyric acid and ethanol: relationships and interactions. Neuropharmacology 1984;23:109–113.
   PubMed Web of Science ®Google Scholar
• Kapitany-Foveny M, Zacher G, Posta J, et al. GHB-involved crimes among intoxicated patients. Forensic Sci Int. 2017;275:23–29.
   PubMed Web of Science ®Google Scholar
• Ortmann LA, Jaeger MW, James LP, et al. Coma in a 20-month-old child from an ingestion of a toy containing 1,4-butanediol, a precursor of gamma-hydroxybutyrate. Pediatr Emerg Care. 2009;25:758–760.
   PubMed Web of Science ®Google Scholar
• De Paepe P, Calle PA, Buylaert WA. Coma induced by intoxication. Handb Clin Neurol. 2008;90:175–191.
   PubMedGoogle Scholar
• Ingels M, Rangan C, Bellezzo J, et al. Coma and respiratory depression following the ingestion of GHB and its precursors: three cases. J Emerg Med. 2000;19:47–50.
   PubMed Web of Science ®Google Scholar
• Thomas G, Bonner S, Gascoigne A. Coma induced by abuse of gamma-hydroxybutyrate (GBH or liquid ecstasy): a case report. BMJ. 1997;314:35–36.
   PubMed Web of Science ®Google Scholar
• Mehling LM, Johansen SS, Wang X, et al. Drug facilitated sexual assault with lethal outcome: GHB intoxication in a six-year-old girl. Forensic Sci Int. 2016;259:e25–e31.
   PubMed Web of Science ®Google Scholar
• Kugelberg FC, Holmgren A, Eklund A, et al. Forensic toxicology findings in deaths involving gamma-hydroxybutyrate. Int J Legal Med. 2010;124:1–6.
   PubMed Web of Science ®Google Scholar
• Zvosec DL, Smith SW, Hall BJ. Three deaths associated with use of Xyrem. Sleep Med. 2009;10:490–493.
   PubMed Web of Science ®Google Scholar
• Abramowitz MZ. GHB and date rape. Br J Psychiatry. 2004;185:176–177.
   PubMed Web of Science ®Google Scholar
• Busardo FP. The importance of hair testing in GHB facilitated sexual assault cases. J Forensic Leg Med. 2016;39:74–75.
   PubMed Web of Science ®Google Scholar
• Winstock A. New health promotion for chemsex and γ-hydroxybutyrate (GHB) ). BMJ. 2015;351:h6281
   PubMedGoogle Scholar
• Couper FJ, Logan BK. GHB and driving impairment. J Forensic Sci. 2001;46:919–923.
   PubMed Web of Science ®Google Scholar
• Jones AW, Holmgren A, Kugelberg FC. Driving under the influence of gamma-hydroxybutyrate (GHB). Forensic Sci Med Pathol. 2008;4:205–211.
   PubMed Web of Science ®Google Scholar
• Corkery JM, Loi B, Claridge H, et al. Gamma hydroxybutyrate (GHB), gamma butyrolactone (GBL) and 1,4-butanediol (1,4-BD; BDO): A literature review with a focus on UK fatalities related to non-medical use. Neurosci Biobehav Rev. 2015;53:52–78.
   PubMed Web of Science ®Google Scholar
• Zvosec DL, Smith SW, Porrata T, et al. Case series of 226 gamma-hydroxybutyrate-associated deaths: lethal toxicity and trauma. Am J Emerg Med. 2011;29:319–332.
   PubMed Web of Science ®Google Scholar
• Dauvilliers Y, Arnulf I, Mignot E. Narcolepsy with cataplexy. Lancet. 2007;369:499–511.
   PubMed Web of Science ®Google Scholar
• Busardo FP, Kyriakou C, Napoletano S, et al. Clinical applications of sodium oxybate (GHB): from narcolepsy to alcohol withdrawal syndrome. Eur Rev Med Pharmacol Sci 2015;19:4654–4663.
   PubMed Web of Science ®Google Scholar
• Gallimberti L, Canton G, Gentile N, et al. Gamma-hydroxybutyric acid for treatment of alcohol withdrawal syndrome. Lancet. 1989;2:787–789.
   PubMed Web of Science ®Google Scholar
• Caputo F, Bernardi M. Sodium oxybate to treat alcohol dependence: 20 years of clinical experience. Addict Biol. 2013;18:901–903.
   PubMed Web of Science ®Google Scholar
• Ingels AS, Wille SM, Samyn N, et al. Screening and confirmation methods for GHB determination in biological fluids. Anal Bioanal Chem. 2014;406:3553–3577.
   PubMed Web of Science ®Google Scholar
• Busardo FP, Kyriakou C, Marchei E, et al. Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) for determination of GHB, precursors and metabolites in different specimens: application to clinical and forensic cases. J Pharm Biomed Anal. 2017;137:123–131.
   PubMed Web of Science ®Google Scholar
• Castro AL, Tarelho S, Dias M, et al. A fast and reliable method for GHB quantitation in whole blood by GC-MS/MS (TQD) for forensic purposes. J Pharm Biomed Anal. 2016;119:139–144.
   PubMed Web of Science ®Google Scholar
• Jones AW, Holmgren A, Kugelberg FC. Gamma-hydroxybutyrate concentrations in the blood of impaired drivers, users of illicit drugs, and medical examiner cases. J Anal Toxicol. 2007;31:566–572.
   PubMed Web of Science ®Google Scholar
• Liechti ME, Quednow BB, Liakoni E, et al. Pharmacokinetics and pharmacodynamics of γ-hydroxybutyrate in healthy subjects. Br J Clin Pharmacol. 2016;81:980–988.
   PubMed Web of Science ®Google Scholar
• Brailsford AD, Cowan DA, Kicman AT. Pharmacokinetic properties of γ-hydroxybutyrate (GHB) in whole blood, serum, and urine. J Anal Toxicol. 2012;36:88–95.
   PubMed Web of Science ®Google Scholar
• Brenneisen R, Elsohly MA, Murphy TP, et al. Pharmacokinetics and excretion of gamma-hydroxybutyrate (GHB) in healthy subjects. J Anal Toxicol. 2004;28:625–630.
   PubMed Web of Science ®Google Scholar
• Ferrara SD, Zotti S, Tedeschi L, et al. Pharmacokinetics of gamma-hydroxybutyric acid in alcohol dependent patients after single and repeated oral doses. Br J Clin Pharmacol. 1992;34:231–235.
   PubMed Web of Science ®Google Scholar
• Scharf MB, Lai AA, Branigan B, et al. Pharmacokinetics of gammahydroxybutyrate (GHB) in narcoleptic patients. Sleep 1998;21:507–514.
   PubMed Web of Science ®Google Scholar
• Abanades S, Farre M, Segura M, et al. Gamma-hydroxybutyrate (GHB) in humans: pharmacodynamics and pharmacokinetics. Ann N Y Acad Sci. 2006;1074:559–576.
   PubMed Web of Science ®Google Scholar
• Jones AW, Eklund A, Kronstrand R. Concentration-time profiles of gamma-hydroxybutyrate in blood after recreational doses are best described by zero-order rather than first-order kinetics. J Anal Toxicol. 2009;33:332–335.
   PubMed Web of Science ®Google Scholar
• Borgen LA, Okerholm R, Morrison D, et al. The influence of gender and food on the pharmacokinetics of sodium oxybate oral solution in healthy subjects. J Clin Pharmacol. 2003;43:59–65.
   PubMed Web of Science ®Google Scholar
• De Paoli G, Walker KM, Pounder DJ. Endogenous γ-hydroxybutyric acid concentrations in saliva determined by gas chromatography-mass spectrometry . J Anal Toxicol. 2011;35:148–152.
   PubMed Web of Science ®Google Scholar
• Raknes G, Aronsen L, Fuskevag OM. Urinary concentrations of gamma-hydroxybutyric acid and related compounds in pregnancy. J Anal Toxicol. 2010;34:394–399.
   PubMed Web of Science ®Google Scholar
• Andresen H, Sprys N, Schmoldt A, et al. Gamma-hydroxybutyrate in urine and serum: additional data supporting current cut-off recommendations. Forensic Sci Int. 2010;200:93–99.
   PubMed Web of Science ®Google Scholar
• Mari F, Politi L, Trignano C, et al. What constitutes a normal ante-mortem urine GHB concentration? J Forensic Leg Med. 2009;16:148–151.
   PubMed Web of Science ®Google Scholar
• Moriya F, Nishimura H, Furumiya J, et al. Effects of drinking and smoking on endogenous levels of urinary gamma-hydroxybutyric acid, a preliminary study. Leg Med. 2006;8:231–234.
   PubMedGoogle Scholar
• Elliott SP, Fais P. Further evidence for GHB naturally occurring in common non-alcoholic beverages. Forensic Sci Int. 2017;277:e36–e38.
   PubMed Web of Science ®Google Scholar
• Brown AJ. Low-carb diets, fasting and euphoria: Is there a link between ketosis and gamma-hydroxybutyrate (GHB)? Med Hypotheses. 2007;68:268–271.
   PubMed Web of Science ®Google Scholar
• Elliott S, Burgess V. The presence of gamma-hydroxybutyric acid (GHB) and gamma-butyrolactone (GBL) in alcoholic and non-alcoholic beverages. Forensic Sci Int. 2005;151:289–292.
   PubMed Web of Science ®Google Scholar
• Jones AW, Hahn RG, Stalberg HP. Distribution of ethanol and water between plasma and whole blood; inter- and intra-individual variations after administration of ethanol by intravenous infusion. Scand J Clin Lab Invest. 1990;50:775–780.
   PubMed Web of Science ®Google Scholar
• Nishimura H, Moriya F, Hashimoto Y. Mechansim of gamma-hydroxybutyic acid production during the early postmortem period. Forensic Toxicol. 2009;27:55–60.
   Web of Science ®Google Scholar
• Kintz P, Villain M, Cirimele V, et al. GHB in postmortem toxicology. Discrimination between endogenous production from exposure using multiple specimens. Forensic Sci Int. 2004;143:177–181.
   PubMed Web of Science ®Google Scholar
• Korb AS, Cooper G. Endogenous concentrations of GHB in postmortem blood from deaths unrelated to GHB use. J Anal Toxicol. 2014;38:582–588.
   PubMed Web of Science ®Google Scholar
• Beránková K, Mutňanská K, Balíková M. Gamma-hydroxybutyric acid stability and formation in blood and urine. Forensic Sci Int. 2006;161:158–162.
   PubMed Web of Science ®Google Scholar
• Moriya F, Hashimoto Y. Site-dependent production of gamma-hydroxybutyric acid in the early postmortem period. Forensic Sci Int. 2005;148:139–142.
   PubMed Web of Science ®Google Scholar
• Biedermann A, Taroni F, Bozza S, et al. Critical analysis of forensic cut-offs and legal thresholds: A coherent approach to inference and decision. Forensic Sci Int. 2018;288:72–80.
   PubMed Web of Science ®Google Scholar
• Zorntlein SW, Kopp A, Becker J, et al. In vitro production of GHB in blood and serum samples under various storage conditions. Forensic Sci Int. 2012;214:113–117.
   PubMed Web of Science ®Google Scholar
• Andresen-Streichert H, Jensen P, Kietzerow J, et al. Endogenous gamma-hydroxybutyric acid (GHB) concentrations in post-mortem specimens and further recommendation for interpretative cut-offs. Int J Legal Med. 2015;129:57–68.
   PubMed Web of Science ®Google Scholar
• Elliott SP. Further evidence for the presence of GHB in postmortem biological fluid: implications for the interpretation of findings. J Anal Toxicol. 2004;28:20–26.
   PubMed Web of Science ®Google Scholar
• Marinetti L, Montgomery MA. The use of GHB to facilitate sexual assault. Forensic Sci Rev. 2010;22:41–59.
   PubMedGoogle Scholar
• Dinis-Oliveira RJ, Magalhaes T. Forensic toxicology in drug-facilitated sexual assault. Toxicol Mech Methods. 2013;23:471–478.
   PubMed Web of Science ®Google Scholar
• EMCDDA. Sexual assaults facilitated by drugs and alcohol. Lisbon: European Monitoring Centre for Drugs and Drug Addiction; 2008.
   Google Scholar
• EMCDDA. GHB and its precursor GBL: an emerging trend case study. Lisbon: European Monitoring Centre for Drugs and Drug Addiction; 2008.
   Google Scholar
• Pichini S, Marchei E, Pacifici R, et al. Chemsex intoxication involving sildenafil as an adulterant of GHB. Drug Test Anal. 2017;9:956–959.
   PubMed Web of Science ®Google Scholar
• Jenkins AJ, editor. Drug testing in alternative biological specimens. New York: Humana Press; 2008, 1–186.
   Google Scholar
• Di Terlizzi R, Platt S. The function, composition and analysis of cerebrospinal fluid in companion animals: part I – function and composition. Vet J. 2006;172:422–431.
   PubMed Web of Science ®Google Scholar
• Sakka L, Coll G, Chazal J. Anatomy and physiology of cerebrospinal fluid. Eur Ann Otorhinolaryngol Head Neck Dis. 2011;128:309–316.
   PubMed Web of Science ®Google Scholar
• Spector R, Robert Snodgrass S, Johanson CE. A balanced view of the cerebrospinal fluid composition and functions: focus on adult humans. Exp Neurol. 2015;273:57–68.
   PubMed Web of Science ®Google Scholar
• Tittarelli R, Pichini S, Pedersen DS, et al. Ultra-high-performance liquid chromatography tandem mass spectrometry determination of GHB, GHB-glucuronide in plasma and cerebrospinal fluid of narcoleptic patients under sodium oxybate treatment. Forensic Sci Int. 2017;274:70–74.
   PubMed Web of Science ®Google Scholar
• Drummer OH. Drug testing in oral fluid. Clin Biochem Rev. 2006;27:147–159.
   PubMedGoogle Scholar
• Schramm W, Smith RH, Craig PA, et al. Drugs of abuse in saliva: a review. J Anal Toxicol. 1992;16:1–9.
   PubMed Web of Science ®Google Scholar
• Proctor GB. The physiology of salivary secretion. Periodontol 2000. 2016;70:11–25.
   PubMed Web of Science ®Google Scholar
• Henson BS, Wong DT. Collection, storage, and processing of saliva samples for downstream molecular applications. Methods Mol Biol 2010;666:21–30.
   PubMedGoogle Scholar
• Langel K, Engblom C, Pehrsson A, et al. Drug testing in oral fluid-evaluation of sample collection devices. J Anal Toxicol. 2008;32:393–401.
   PubMed Web of Science ®Google Scholar
• Langel K, Gjerde H, Favretto D, et al. Comparison of drug concentrations between whole blood and oral fluid. Drug Test Anal. 2014;6:461–471.
   PubMed Web of Science ®Google Scholar
• Mucklow JC, Bending MR, Kahn GC, et al. Drug concentration in saliva. Clin Pharmacol Ther. 1978;24:563–570.
   PubMed Web of Science ®Google Scholar
• Kintz P, Goulle JP, Cirimele V, et al. Window of detection of gamma-hydroxybutyrate in blood and saliva. Clin Chem. 2001;47:2033–2034.
   PubMed Web of Science ®Google Scholar
• Abanades S, Farre M, Segura M, et al. Disposition of gamma-hydroxybutyric acid in conventional and nonconventional biologic fluids after single drug administration: issues in methodology and drug monitoring. Ther Drug Monit. 2007;29:64–70.
   PubMed Web of Science ®Google Scholar
• Krotulski AJ, Mohr ALA, Friscia M, et al. Field detection of drugs of abuse in oral fluid using the Alere™ DDS®2 Mobile Test System with Confirmation by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). J Anal Toxicol. 2018;42:170–176.
   PubMed Web of Science ®Google Scholar
• Logan BK, D’Orazio AL, Mohr ALA, et al. Recommendations for toxicological investigation of drug-impaired driving and motor vehicle fatalities-2017 update. J Anal Toxicol. 2018;42:63–68.
   PubMed Web of Science ®Google Scholar
• Bevalot F, Cartiser N, Bottinelli C, et al. Vitreous humor analysis for the detection of xenobiotics in forensic toxicology: a review. Forensic Toxicol. 2016;34:12–40.
   PubMed Web of Science ®Google Scholar
• Metushi IG, Fitzgerald RL, McIntyre IM. Assessment and comparison of vitreous humor as an alternative matrix for forensic toxicology screening by GC-MS. J Anal Toxicol. 2016;40:243–247.
   PubMed Web of Science ®Google Scholar
• Skopp G. Postmortem toxicology. Forensic Sci Med Pathol. 2010;6:314–325.
   PubMed Web of Science ®Google Scholar
• Dinis-Oliveira RJ, Carvalho F, Duarte JA, et al. Collection of biological samples in forensic toxicology. Toxicol Mech Methods. 2010;20:363–414.
   PubMed Web of Science ®Google Scholar
• Flanagan RJ, Connally G, Evans JM. Analytical toxicology: guidelines for sample collection postmortem. Toxicol Rev. 2005;24:63–71.
   PubMedGoogle Scholar
• Marinetti LJ, Isenschmid DS, Hepler BR, et al. Analysis of GHB and 4-methyl-GHB in postmortem matrices after long-term storage. J Anal Toxicol. 2005;29:41–47.
   PubMed Web of Science ®Google Scholar
• Castro AL, Dias M, Reis F, et al. Gamma-hydroxybutyric acid endogenous production and post-mortem behaviour - The importance of different biological matrices, cut-off reference values, sample collection and storage conditions. J Forensic Leg Med. 2014;27:17–24.
   PubMed Web of Science ®Google Scholar
• Busardo FP, Portelli F, Montana A, et al. When a death apparently associated to sexual assault is instead a natural death due to idiopathic hypereosinophilic syndrome: the importance of gamma-hydroxybutyric acid analysis in vitreous humor. Leg Med. 2017;26:92–97.
   PubMed Web of Science ®Google Scholar
• Busardo FP, Mannocchi G, Giorgetti R, et al. Stability of endogenous GHB in vitreous humor vs peripheral blood in dead bodies. Forensic Sci Int. 2017;274:64–69.
   PubMed Web of Science ®Google Scholar
• Hotham N, Hotham E. Drugs in breastfeeding. Aust Prescr. 2015;38:156–159.
   PubMed Web of Science ®Google Scholar
• Haastrup MB, Pottegard A, Damkier P. Alcohol and breastfeeding. Basic Clin Pharmacol Toxicol. 2014;114:168–173.
   PubMed Web of Science ®Google Scholar
• Ho E, Collantes A, Kapur BM, et al. Alcohol and breast feeding: calculation of time to zero level in milk. Biol Neonate. 2001;80:219–222.
   PubMedGoogle Scholar
• Busardo FP, Bertol E, Mannocchi G, et al. Determination of GHB levels in breast milk and correlation with blood concentrations. Forensic Sci Int. 2016;265:172–181.
   PubMed Web of Science ®Google Scholar
• Busardo FP, Pichini S. GHB concentration in breast milk of narcoleptic women treated with sodium oxybate. How important it is to be careful when interpreting values. Sleep Med. 2017;40:129.
   PubMed Web of Science ®Google Scholar
• Barker EC, Puchowicz M, Letterio J, et al. GHB levels in breast milk of women with narcolepsy with cataplexy treated with sodium oxybate. Sleep Med. 2017;36:172–177.
   PubMed Web of Science ®Google Scholar
• Carter LP, Koek W, France CP. Behavioral analyses of GHB: receptor mechanisms. Pharmacol Ther. 2009;121:100–114.
   PubMed Web of Science ®Google Scholar
• Mardal M, Johansen SS, Thomsen R, et al. Advantages of analyzing postmortem brain samples in routine forensic drug screening-case series of three non-natural deaths tested positive for lysergic acid diethylamide (LSD). Forensic Sci Int. 2017;278:e14–e18.
   PubMed Web of Science ®Google Scholar
• Thomsen R, Rasmussen BS, Johansen SS, et al. Postmortem concentrations of gamma-hydroxybutyrate (GHB) in peripheral blood and brain tissue – differentiating between postmortem formation and antemortem intake. Forensic Sci Int. 2017;272:154–158.
   PubMed Web of Science ®Google Scholar
• Nedahl M, Johansen SS, Linnet K. Reference brain/blood concentrations of citalopram, duloxetine, mirtazapine and sertraline. J Anal Toxicol. 2018;42:149–156.
   PubMed Web of Science ®Google Scholar
• Doherty JD, Hattox SE, Snead OC, et al. Identification of endogenous gamma-hydroxybutyrate in human and bovine brain and its regional distribution in human, guinea pig and rhesus monkey brain. J Pharmacol Exp Ther. 1978;207:130–139.
   PubMed Web of Science ®Google Scholar
• Ferrara SD, Tedeschi L, Frison G, et al. Fatality due to gamma-hydroxybutyric acid (GHB) and heroin intoxication. J Forensic Sci. 1995;40:501–504.
   PubMed Web of Science ®Google Scholar
• Kalasinsky KS, Dixon MM, Schmunk GA, et al. Blood, brain, and hair GHB concentrations following fatal ingestion. J Forensic Sci. 2001;46:728–730.
   PubMed Web of Science ®Google Scholar
• Mazarr-Proo S, Kerrigan S. Distribution of GHB in tissues and fluids following a fatal overdose. J Anal Toxicol. 2005;29:398–400.
   PubMed Web of Science ®Google Scholar
• Nielsen MKK, Nedahl M, Johansen SS, et al. Validation of a fully automated solid-phase extraction and ultra-high-performance liquid chromatography-tandem mass spectrometry method for quantification of 30 pharmaceuticals and metabolites in post-mortem blood and brain samples. Drug Test Anal. 2018;10:1147–1157.
   Web of Science ®Google Scholar
• Stimpfl T, Drugs-of-abuse testing in brain. In: Jenkins AJ, editor. Drug testing in alternative biological specimens. New York: Humana Press;2008. p. 157–180.
   Google Scholar
• White CM. Pharmacologic, Pharmacokinetic, and Clinical Assessment of Illicitly Used gamma-Hydroxybutyrate. J Clin Pharmacol. 2017;57:33–39.
   PubMed Web of Science ®Google Scholar
• Kintz P, Salomone A, Vincenti M, editors. Hair analysis in clinical and forensic toxicology. London: Academic Press;2015.
   Google Scholar
• Villain M, Cirimele V, Kintz P. Hair analysis in toxicology. Clin Chem Lab Med. 2004;42:1265–1272.
   PubMed Web of Science ®Google Scholar
• Cuypers E, Flanagan RJ. The interpretation of hair analysis for drugs and drug metabolites. Clin Toxicol. 2018;56:90–100.
   PubMed Web of Science ®Google Scholar
• Pragst F, Balikova MA. State of the art in hair analysis for detection of drug and alcohol abuse. Clin Chim Acta. 2006;370:17–49.
   PubMed Web of Science ®Google Scholar
• Wang X, Drummer OH. Review: interpretation of drug presence in the hair of children. Forensic Sci Int. 2015;257:458–472.
   PubMed Web of Science ®Google Scholar
• Petzel-Witt S, Pogoda W, Wunder C, et al. Influence of bleaching and coloring on ethyl glucuronide content in human hair. Drug Test Anal. 2018;10:177–183.
   PubMed Web of Science ®Google Scholar
• Wang X, Linnet K, Johansen SS. Development of a UPLC–MS/MS method for determining γ-hydroxybutyric acid (GHB) and GHB glucuronide concentrations in hair and application to forensic cases. Forensic Toxicol. 2016;34:51–60.
   Web of Science ®Google Scholar
• Bertol E, Mari F, Vaiano F, et al. Determination of GHB in human hair by HPLC-MS/MS: Development and validation of a method and application to a study group and three possible single exposure cases. Drug Test Analysis. 2015;7:376–384.
   PubMed Web of Science ®Google Scholar
• Busardo FP, Vaiano F, Mannocchi G, et al. Twelve months monitoring of hair GHB decay following a single dose administration in a case of facilitated sexual assault. Drug Test Anal. 2017;9:953–956.
   PubMed Web of Science ®Google Scholar
• Kintz P, Cirimele V, Jamey C, et al. Testing for GHB in hair by GC/MS/MS after a single exposure. Application to document sexual assault. J Forensic Sci. 2003;48:195–200.
   PubMed Web of Science ®Google Scholar
• Busardo FP, Pichini S, Zaami S, et al. Hair testing of GHB: an everlasting issue in forensic toxicology. Clin Chem Lab Med. 2018;56:198–208.
   PubMed Web of Science ®Google Scholar
• Kintz P. A novel approach to document single exposure to GHB: hair analysis after sweat contamination. J Anal Toxicol. 2016;40:563–564.
   PubMed Web of Science ®Google Scholar
• Kintz P. Value of hair analysis in postmortem toxicology. Forensic Sci Int. 2004;142:127–134.
   PubMed Web of Science ®Google Scholar
• Castro AL, Tarelho S, Dias M, et al. Comparison of endogenous GHB concentrations in blood and hair in death cases with emphasis on the post mortem interval. Int J Legal Med. 2016;130:959–965.
   PubMed Web of Science ®Google Scholar
• Petersen IN, Tortzen C, Kristensen JL, et al. Identification of a new metabolite of GHB: gamma-hydroxybutyric acid glucuronide. J Anal Toxicol. 2013;37:291–297.
   PubMed Web of Science ®Google Scholar
• Mehling LM, Wang X, Johansen SS, et al. Determination of GHB and GHB-beta-O-glucuronide in hair of three narcoleptic patients-comparison between single and chronic GHB exposure. Forensic Sci Int. 2017;278:e8–e13.
   PubMed Web of Science ®Google Scholar
• Piper T, Mehling LM, Spottke A, et al. Potential of GHB phase-II-metabolites to complement current approaches in GHB post administration detection. Forensic Sci Int. 2017;279:157–164.
   PubMed Web of Science ®Google Scholar
• Palmer RB. A review of the use of ethyl glucuronide as a marker for ethanol consumption in forensic and clinical medicine. Semin Diagn Pathol. 2009;26:18–27.
   PubMed Web of Science ®Google Scholar
• Skipper GE, Weinmann W, Thierauf A, et al. Ethyl glucuronide: a biomarker to identify alcohol use by health professionals recovering from substance use disorders. Alcohol Alcohol. 2004;39:445–449.
   PubMed Web of Science ®Google Scholar
• Hanisch S, Stachel N, Skopp G. A potential new metabolite of gamma-hydroxybutyrate: sulfonated gamma-hydroxybutyric acid. Int J Legal Med. 2016;130:411–414.
   PubMed Web of Science ®Google Scholar
• Busardo FP, Pichini S. The everlasting issue of GHB cut-offs in biological samples: How important it is to be careful when interpreting values. Forensic Sci Int. 2017;279:e14–e15.
   PubMed Web of Science ®Google Scholar
• Stove CP, Ingels AS, De Kesel PM, et al. Dried blood spots in toxicology: from the cradle to the grave?. Crit Rev Toxicol. 2012;42:230–243.
   PubMed Web of Science ®Google Scholar
• Sadones N, Capiau S, De Kesel PM, et al. Spot them in the spot: analysis of abused substances using dried blood spots. Bioanalysis 2014;6:2211–2227.
   PubMed Web of Science ®Google Scholar
• Sadones N, Archer JR, Ingels AS, et al. Do capillary dried blood spot concentrations of gamma-hydroxybutyric acid mirror those in venous blood? A comparative study. Drug Test Anal. 2015;7:336–340.
   PubMed Web of Science ®Google Scholar
• Drummer OH. Requirements for bioanalytical procedures in postmortem toxicology. Anal Bioanal Chem. 2007;388:1495–1503.
   PubMed Web of Science ®Google Scholar
• Arndt T. Urine-creatinine concentration as a marker of urine dilution: reflections using a cohort of 45,000 samples. Forensic Sci Int. 2009;186:48–51.
   PubMed Web of Science ®Google Scholar
• Chaturvedi AK, Sershon JL, Craft KJ, et al. Effects of fluid load on human urine characteristics related to workplace drug testing. J Anal Toxicol. 2013;37:5–10.
   PubMed Web of Science ®Google Scholar
• Crookes CE, Faulds MC, Forrest AR, et al. A reference range for endogenous gamma-hydroxybutyrate in urine by gas chromatography-mass spectrometry. J Anal Toxicol. 2004;28:644–649.
   PubMed Web of Science ®Google Scholar
• Elian AA. Determination of endogenous gamma-hydroxybutyric acid (GHB) levels in antemortem urine and blood. Forensic Sci Int. 2002;128:120–122.
   PubMed Web of Science ®Google Scholar
• Brailsford AD, Cowan DA, Kicman AT. Urinary gamma-hydroxybutyrate concentrations in 1126 female subjects. J Anal Toxicol. 2010;34:555–561.
   PubMed Web of Science ®Google Scholar
• LeBeau MA, Montgomery MA, Morris-Kukoski C, et al. A comprehensive study on the variations in urinary concentrations of endogenous gamma-hydroxybutyrate (GHB). J Anal Toxicol. 2006;30:98–105.
   PubMed Web of Science ®Google Scholar
• Yeatman DT, Reid K. A study of urinary endogenous gamma-hydroxybutyrate (GHB) levels. J Anal Toxicol. 2003;27:40–42.
   PubMed Web of Science ®Google Scholar
• Gambino R. Sodium fluoride: an ineffective inhibitor of glycolysis. Ann Clin Biochem. 2013;50:3–5.
   PubMed Web of Science ®Google Scholar
• Fjeld B, Burns ML, Karinen R, et al. Long-term stability of GHB in post-mortem samples and samples from living persons, stored at −20 degrees C, using fluoride preservatives. Forensic Sci Int. 2012;222:47–51.
   PubMed Web of Science ®Google Scholar
• Kerrigan S. In vitro production of gamma-hydroxybutyrate in antemortem urine samples. J Anal Toxicol. 2002;26:571–574.
   PubMed Web of Science ®Google Scholar
• Schrock A, Hari Y, Konig S, et al. Pharmacokinetics of GHB and detection window in serum and urine after single uptake of a low dose of GBL – an experiment with two volunteers. Drug Test Anal. 2014;6:363–366.
   PubMed Web of Science ®Google Scholar
• Moriya F, Hashimoto Y. Endogenous gamma-hydroxybutyric acid levels in postmortem blood. Leg Med (Tokyo). 2004;6:47–51.
   PubMedGoogle Scholar
• Jones AW, Holmgren A, Kugelberg FC, et al. Relationship between postmortem urine and blood concentrations of GHB furnishes useful information to help interpret drug intoxication deaths. J Anal Toxicol. 2018;42. Available from: https://doi.org/10.1093/jat/bky041
   PubMed Web of Science ®Google Scholar
• Jones AW, Holmgren P. Urine/blood ratios of ethanol in deaths attributed to acute alcohol poisoning and chronic alcoholism. Forensic Sci Int. 2003;135:206–212.
   PubMed Web of Science ®Google Scholar
• Jones AW. Ethanol distribution ratios between urine and capillary blood in controlled experiments and in apprehended drinking drivers. J Forensic Sci. 1992;37:13208J– 132034.
   Web of Science ®Google Scholar
• Cappelle D, De Keukeleire S, Neels H, et al. Keratinous matrices for the assessment of drugs of abuse consumption: A correlation study between hair and nails. Drug Test Anal. 2018;10:1110–1118.
   Web of Science ®Google Scholar
• Maurer HH. Impact of pharmacogenomic variations and/or drug-drug (food) interctions on interpretation of clinical and forensic toxicology cases with CNS acting drugs. TIAFT Bull. 2018;48:27–30.
   Google Scholar