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Mycologia Volume 94, 2002 - Issue 2
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Original Articles

Utility of cytoplasmic fluorescent proteins for live-cell imaging of Magnaporthe grisea in planta

Kirk J. Czymmek Department of Biological Sciences, University of Delaware, Newark, Delaware 19716
,
Timothy M. Bourett DuPont Crop Genetics, PO Box 80402, Wilmington, Delaware 19880-0402
,
James A. Sweigard
,
Anne Carroll DuPont Crop Genetics, Delaware Technology Park, Newark, Delaware 19713
&
Richard J. Howard DuPont Crop Genetics, PO Box 80402, Wilmington, Delaware 19880-0402
Pages 280-289 | Accepted 26 Aug 2001, Published online: 31 Jan 2017

  • Bae Y, Knudsen GR., 2000 Cotransformation of Trichoderma harzianum with β-glucuronidase and green fluorescent protein genes provides a useful tool for monitoring fungal growth and activity in natural soils. Environ Microbiol 66:810-815
  • Berteaux-Lecellier V, Zickler D, Debuchy R, Panvier-Adoutte A, Thompson-Coffe C, Picard M., 1998 A homologue of the yeast SHE4 gene is essential for the transition between the syncytial and cellular stages during sexual reproduction of the fungus Podospora anserina. EMBO J 17:1248-1258
  • Boevink P, Oparka K, Santa Cruz S, Martin B, Betteridge A, Hawes C., 1998 Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network. Plant J 15:441-447
  • Bourett TM, Czymmek KJ, Dezwaan TM, Sweigard JA, Howard RJ., 2000 Localization and visualization of specific gene products in fungal plant pathogens and their hosts. Microsc Microanal 6:680-681
  • Bourett TM, Czymmek KJ, Howard RJ., 1998 An improved method for affinity probe localization in whole cells of filamentous fungi. Fungal Genet Biol 24:3-13
  • Bowyer P, Mueller E, Lucas J., 2000 Use of an isocitrate lyase promoter-GFP fusion to monitor carbon metabolism of the plant pathogen Tapesia yallundae during infection of wheat. Mol Plant Pathol 1:253-262
  • Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC., 1994 Green fluorescent protein as a marker for gene expression. Science 263:802-805
  • Cormack B., 1998 Green fluorescent protein as a reporter of transcription and protein localization in fungi. Curr Opin Microbiol 1:406-410
  • Czymmek KJ, Bourett TM, Howard RJ., 1996 Immunolocalization of tubulin and actin in thick-sectioned fungal hyphae after freeze-substitution and methacrylate de-embedment. J Microsc 181:153-161
  • Czymmek KJ, Whallon JH, Klomparens KL., 1994 Confocal microscopy in mycological research. Exp Mycol 18:275-293.
  • DeZwaan TM, Carroll AM, Valent B, Sweigard JA., 1999 Magnaporthe grisea Pth11p is a novel plasma membrane protein that mediates appressorium differentiation in response to inductive substrate cues. Plant Cell 11:2013-2030
  • Dumas B, Centis S, Sarrazin N, Esquerre-Tugaye M-T., 1999 Use of green fluorescent protein to detect expression of an endopolygalacturonase gene of Colletotrichum lindemuthianum during bean infection. Appl Environ Microbiol 65:1769-1771
  • Duncan KE, Howard RJ., 2000 Cytological analysis of wheat infection by the leaf blotch pathogen Mycosphaerella graminicola. Mycol Res 104:1074-1082
  • Fernandez-Abalos JM, Fox H, Pitt C, Wells B, Doonan JH., 1998 Plant-adapted green fluorescent protein is a versatile vital reporter for gene expression, protein localization and mitosis in the filamentous fungus, Aspergillus nidulans. Mol Microbiol 27:121-130
  • Gordon CL, Archer DB, Jeenes DJ, Doonan JH, Wells B, Trinci APJ, Robson GD., 2000 A glucoamylase::GFP gene fusion to study protein secretion by individual hyphae of Aspergillus niger. J Microbiol Methods 42:39-48
  • Hardham AR, Mitchell HJ., 1998 Use of molecular cytology to study the structure and biology of phytopathogenic and mycorrhizal fungi. Fungal Genet Biol 24:252-284
  • Heath MC., 2000 Hypersensitive response-related death. Plant Mol Biol 44:321-334
  • Heath MC, Valent B, Howard RJ, Chumley FG., 1990 Correlations between cytologically detected plant-fungal interactions and pathogenicity of Magnaporthe grisea toward weeping lovegrass. Phytopathology 80:1382-1386.
  • Heim R, Tsien RY., 1996 Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol 6:178-182
  • Howard RJ., 2001 Cytology of fungal pathogens and plant-host interactions. Curr Opin Microbiol 4:365-373
  • Kawamura E., 1940 Reaction of certain species of the genus Oryza to the infection of Pyricularia oryzae. Bull Sci Fac Terkult Kyusyu Imp Univ 9:157-166
  • Koga H., 1994 Hypersensitive death, autofluorescence, and ultrastructural changes in cells of leaf sheaths of susceptible and resistant near-isogenic lines of rice (Pi-zt) in relation to penetration and growth of Pyricularia oryzae. Can J Bot 72:1463-1477
  • Koga H, Horino O, Kobayashi T., 1986 Ultrastructural studies on the resistance mechanisms of rice plants infected with Pyricularia oryzae Cav. Bull Hok Nation Agr Exp Sta 28:1-56
  • König K., 2000 Multiphoton microscopy in life sciences. J Microsc 200:83-104
  • Lehmler C, Steinberg G, Snetselaar KM, Schliwa M, Kahmann R, Bolker M., 1997 Identification of a motor protein required for filamentous growth in Ustilago maydis. EMBO J 16:3464-3473
  • Marc J, Granger CL, Brincat J, Fisher D, Kao T, McCubbin AG, Cyr RJ., 1998 A GFP-MAP4 reporter gene for visualizing cortical microtubule rearrangements in living epidermal cells. Plant Cell 10:1927-1939
  • Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov SA., 1999 Fluorescent proteins from nonbioluminescent Anthozoa species. Nature Biotechnol 17:969-973
  • Nielsen K, Olsen O, Oliver R., 1999 A transient expression system to assay putative antifungal genes on powdery mildew infected barley leaves. Physiol Mol Plant Pathol 54:1-12
  • Oldenburg KR, Vo KT, Michaelis S, Paddon C., 1997 Recombination-mediated PCR-directed plasmid construction in vivo in yeast. Nucleic Acids Res 25:451-452
  • Peng YL, Shishiyama J., 1988 Temporal sequence of cytological events in rice leaves infected with Pyricularia oryzae. Can J Bot 66:730-735
  • Reichel C, Mathur J, Eckes P, Langenkemper K, Koncz C, Schell J, Reiss B, Maas C., 1996 Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono- and dicotyledonous plant cells. Proc Natl Acad Sci USA 93:5888-5893.
  • Sheen J, Hwang S, Niwa Y, Kobayashi H, Galbraith DW., 1995 Green-fluorescent protein as a new vital marker in plant cells. Plant J 8:777-784
  • Shirasu K, Nielsen K, Piffanelli P, Oliver R, Schulze-Lefert P., 1999 Cell-autonomous complementation of mlo resistance using a biolistic transient expression system. Plant J 17:293-299
  • Siedenberg D, Mestric S, Ganzlin M, Schmidt M, Punt PJ, van den Hondel CAMJJ, Rinas U., 1999 GlaA promoter controlled production of a mutant green fluorescent protein (S65T) by recombinant Aspergillus niger during growth on defined medium in batch and fed-batch cultures. Biotechnol Prog 15:43-50
  • Sievers N, Bertsch E, Fischer R., 1999 Isolation of nuclear migration mutants of Aspergillus nidulans using GFP expressing strains. Mycol Res 103:961-966
  • Skalamera D, Heath MC., 1998 Changes in the cytoskeleton accompanying infection-induced nuclear movements and the hypersensitive response in plant cells invaded by rust fungi. Plant J 16:191-200
  • Snyder BA, Nicholson RL., 1990 Synthesis of phytoalexins in sorghum as a site-specific response to fungal ingress. Science 248:1637-1639
  • Spellig T, Bottin A, Kahmann R., 1996 Green fluorescent protein (GFP) as a new vital marker in the phytopathogenic fungus Ustilago maydis. Mol Gen Genet 252:503-509
  • Stephenson SA, Hatfield J, Rusu AG, Maclean DJ, Manners JM., 2000 CgDN3: an essential pathogenicity gene of Colletotrichum gloesporiodes necessary to avert a hypersensitive-like response in the host Stylosanthes guianensis. Mol Plant Microbe Interact 13:929-941
  • Suelmann R, Sievers N, Galetzka D, Robertson L, Timberlake WE, Fischer R., 1998 Increased nuclear traffic chaos in hyphae of Aspergillus nidulans: molecular characterization of aspB and in vivo observation of nuclear behaviour. Mol Microbiol 30:831-842
  • ———, Fischer R., 2000 Mitochondrial movement and morphology depend on an intact actin cytoskeleton in Aspergillus nidulans. Cell Motil Cytoskeleton 45:42-50
  • Sweigard J, Carroll A, Kang S, Farrall L, Chumley F, Valent B., 1995 Identification, cloning, and characterization of PWL2, a gene for host species specificity in the rice blast fungus. Plant Cell 7:1221-1233
  • Tirlapur UK, König K., 1999 Near-infrared femtosecond laser pulses as a novel non-invasive means for dye-permeation and 3-D imaging of localized dye-coupling in the Arabidopsis root meristem. Plant J 20:363-370
  • Valdez-Taubas J, Diallinas G, Scazzocchio C, Rosa AL., 2000 Protein expression and subcellular localization of the general purine transporter UapC from Aspergillus nidulans. Fungal Genet Biol 30:105-113
  • Valent B, Farrall L, Chumley F., 1991 Magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses. Genetics 127:87-101
  • van West P, Reid B, Campbell TA, Sandrock RW, Fry WE, Kamoun S, Gow NAR., 1999 Green fluorescent protein (GFP) as a reporter gene for the plant pathogenic oomycete Phytophthora palmivora. FEMS Microbiol Lett 178:71-80
  • Vautard-Mey G, Cotton P, Fevre M., 1999 Expression and compartmentation of the glucose repressor CRE1 from the phytopathogenic fungus Sclerotinia sclerotiorum. Eur J Biochem 266:252-259
  • Vierheilig H, Bockenhoff A, Knoblauch M, Juge C, Van Bel AJE, Grundler F, Piche Y, Wyss U., 1999 In vivo observations of the arbuscular mycorrhizal fungus Glomus mosseae in roots by confocal laser scanning microscopy. Mycol Res 103:311-314
  • Xiang X, Han G, Winkelmann DA, Zuo W, Morris NR., 2000 Dynamics of cytoplasmic dynein in living cells and the effect of mutation in the dynactin complex actin-related protein Arp1. Curr Biol 10:603-606
  • Yamashita RA, Osherov N, May GS., 2000 Localization of wildtype and mutant class I myosin proteins in Aspergillus nidulans using GFP-fusion proteins. Cell Motil Cytoskeleton 45:163-172
  • Zeilinger S, Galhaup C, Payer K, Woo SL, Mach RL, Fekete C, Lorito M, Kubicek CP., 1999 Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fungal Genet Biol 26:131-140

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