Advanced search
Publication Cover
Mycologia Volume 98, 2006 - Issue 3
Submit an article Journal homepage
107
Views
7
CrossRef citations to date
0
Altmetric
Original Articles

5-fluoro-orotic acid induces chromosome alterations in genetically manipulated strains of Candida albicans

Melanie Wellington Department of Pediatrics, University of Rochester Medical Center, Rochester, New York 14642
,
M. Anaul
&
Kabir Elena Rustchenko Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642Correspondence[email protected]
Pages 393-398 | Accepted 24 Mar 2006, Published online: 23 Jan 2017

LITERATURE CITED

  • Cannon RD, Jenkinson HF, Shepherd MG. 1992. Cloning and expression of Candida albicans ADE2 and proteinase genes on a replicative plasmid in C. albicans and Saccharomyces cerevisiae. Mol Gen Genet 235: 453–457.
  • Chen X, Magee BB, Dawson D, Magee PT, Kumamoto CA. 2004. Chromosome 1 trisomy compromises the virulence of Candida albicans. Mol Microbiol 51:551–565.
  • Fonzi WA, Irwin MY. 1993. Isogenic strain construction and gene mapping in Candida albicans. Genetics 134: 717–728.
  • Forche A, May G, Beckerman J, Kauffman S, Becker J, Magee PT. 2003. A system for studying genetic changes in Candida albicans during infection. Fungal Genet Biol 39:38–50.
  • Gorman JA, Chan W, Gorman JW. 1991. Repeated use of GAL1 for gene disruption in Candida albicans. Genetics 129:19–24.
  • Hiraoka M, Watanabe K-I, Umezu K, Maki H. 2000. Spontaneous loss of heterozygosity in diploid Saccharomyces cerevisiae cells. Genetics 156:1531–1548.
  • Janbon G, Sherman F, Rustchenko E. 1998. Monosomy of a specific chromosome determines L-sorbose utilization: a novel regulatory mechanism in Candida albicans. Proc Natl Acad Sci USA 95:5150–5155.
  • Kabir MA, Rustchenko E. 2005. Determination of gaps by contig alignment with telomere-mediated chromosomal fragmentation in Candida albicans. Gene 345: 279–287.
  • Morschhäuser J, Michel S, Staib P. 1999. Sequential gene disruption in Candida albicans by FLP-mediated site-specific recombination. Mol Microbiol 32:547–556.
  • Noble S, Johnson AD. 2005. Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans. Eukaryotic Cell 4:298–309.
  • Perepnikhatka V, Fischer FJ, Niimi M, Baker RA, Cannon RD, Wang YK, Sherman F, Rustchenko E. 1999. Specific chromosome alterations in fluconazole-resistant mutants of Candida albicans. J Bacteriol 181:4041–4049.
  • Rustchenko EP. 2003. Candida albicans adaptability to environmental challenges by means of specific chromosome alteration. In: Pandalai SG, ed. Recent research developments in bacteriology. Trivandrum, India: Transworld Research Network. 1:91–102.
  • ———, Curran TM, Sherman F. 1993. Variations in the number of ribosomal DNA units in morphological mutants and normal strains of Candida albicans and in normal strains of Saccharomyces cerevisiae. J Bacteriol 175:7189–7199.
  • ———, Howard DH, Sherman F. 1994. Chromosome alterations of Candida albicans are associated with the gain and loss of assimilating functions. J Bacteriol 176:3231–3241.
  • Rustchenko E, Sherman F. 2002. Genetic instability of Candida albicans. In: Howard DH, ed. Fungi pathogenic for humans and animals. New York: Marcel Dekker Inc. p 723–776.
  • Rustchenko-Bulgac EP, Sherman F, Hicks JB. 1990. Chromosome rearrangements associated with morphological mutants provide a means for genetic variation of Candida albicans. J Bacteriol 172:1276–1283.
  • ———, Howard DH. 1993. Multiple chromosome and phenotypic changes in spontaneous mutants of Candida albicans. J Gen Microbiol 139:1195–1207.
  • Sherman F. 2002. Getting started with yeast. Meth Enzymol 350:3–41.
  • Wang Y-K, Das B, Huber DH, Wellington M, Kabir A, Sherman F, Rustchenko E. 2004. Role of the 14-3-3 protein in carbon metabolism of the pathogenic yeast Candida albicans. Yeast 21:685–702.
  • Wellington M, Rustchenko E. 2005. 5-fluoro-orotic acid induces chromosome alterations in Candida albicans. Yeast 22:57–70.
  • Wirsching S, Michel S, Morschhäuser J. 2000. Targeted gene disruption in Candida albicans wild-type strains: the role of the MDR1 gene in fluconazole resistance of clinical Candida albicans isolates. Mol Microbiol 36:856–865.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.