Global DNA (hydroxy)methylation is stable over time under several storage conditions and temperatures

References

• Felsenfeld G. A brief history of epigenetics. Cold Spring Harb Perspect Biol. 2014;6. DOI:10.1101/cshperspect.a018200
   Google Scholar
• Moore LD, Le T, Fan G. DNA methylation and its basic function. Neuropsychopharmacology. 2013;38(1):23–38.
   Google Scholar
• Melamed P, Yosefzon Y, David C, et al. Variants, and differential effects on function. Front Cell Dev Biol. 2018;6:22.
   Google Scholar
• Pfeifer GP, Kadam S, Jin S-G. 5-hydroxymethylcytosine and its potential roles in development and cancer. Epigenetics Chromatin. 2013;6(1):10.
   Google Scholar
• Henderson-Smith A, Fisch KM, Hua J, et al. DNA methylation changes associated with Parkinson’s disease progression: outcomes from the first longitudinal genome-wide methylation analysis in blood. Epigenetics. 2019;14(4):365–382.
   Google Scholar
• Jiang D, Sun M, You L, et al. DNA methylation and hydroxymethylation are associated with the degree of coronary atherosclerosis in elderly patients with coronary heart disease. Life Sci. 2019;224:241–248.
   Google Scholar
• Ciccarone F, Valentini E, Malavolta M, et al. DNA hydroxymethylation levels are altered in blood cells from down syndrome persons enrolled in the MARK-AGE project. Journals Gerontol Ser A. 2018;73:737–744.
   Google Scholar
• Zouali M. Epigenetics of autoimmune diseases. Autoimmune Dis. 2020:429–466 Elsevier. doi:10.1016/b978-0-12-812102-3.00025-7
   Google Scholar
• Skvortsova K, Stirzaker C, Taberlay P. The DNA methylation landscape in cancer. Essays Biochem. 2019;63(6):797–811.
   Google Scholar
• Gosselt HR, van Zelst BD, de Rotte MCFJ, et al. Higher baseline global leukocyte DNA methylation is associated with MTX non-response in early RA patients. Arthritis research & therapy. 2019;21(1):157. DOI:10.1186/s13075-019-1936-5
   Google Scholar
• Ciechomska, M., Roszkowski, L., & Maslinski, W. DNA methylation as a future therapeutic and diagnostic target in rheumatoid arthritis. Cells. 2019;8(9):953.
   Google Scholar
• Talens RP, Boomsma DI, Tobi EW, et al. Variation, patterns, and temporal stability of DNA methylation: considerations for epigenetic epidemiology. Faseb J. 2010;24(9):3135–3144.
   Google Scholar
• Zaimi I, Pei D, Koestler DC, et al. Variation in DNA methylation of human blood over a 1-year period using the Illumina MethylationEPIC array. Epigenetics. 2018;13(10–11):1056–1071.
   Google Scholar
• Wang Y, Zheng H, Chen J, et al. The impact of different preservation conditions and freezing-thawing cycles on quality of RNA, DNA, and proteins in cancer tissue. Biopreserv Biobank. 2015;13(5):335–347.
   Google Scholar
• Li Y, Pan X, Roberts ML, et al. Stability of global methylation profiles of whole blood and extracted DNA under different storage durations and conditions. Epigenomics. 2018;10:797–811.
   Google Scholar
• Huang LH, Lin PH, Tsai KW, et al. The effects of storage temperature and duration of blood samples on DNA and RNA qualities. PLoS One. 2017;12. DOI:10.1371/journal.pone.0184692.
   Google Scholar
• Schröder C, Steimer W. gDNA extraction yield and methylation status of blood samples are affected by long-term storage conditions. PLoS One. 2018;13. DOI:10.1371/journal.pone.0192414
   Google Scholar
• Bulla A, De Witt B, Ammerlaan W, et al. Blood DNA yield but not integrity or methylation is impacted after long-term storage. Biopreserv Biobank. 2016;14(1):29–38.
   Google Scholar
• Coppieters N, Dieriks BV, Lill C, et al. Global changes in DNA methylation and hydroxymethylation in Alzheimer’s disease human brain. Neurobiol Aging. 2014;35:1334–1344.
   Google Scholar
• Song L, James SR, Kazim L, et al. Specific method for the determination of genomic DNA methylation by liquid chromatography-electrospray ionization tandem mass spectrometry. Anal Chem. 2005;77(2):504–510.
   Google Scholar
• Kok, R. M., Smith, D. E., Barto, R., et al. Global DNA methylation measured by liquid chromatography-tandem mass spectrometry: analytical technique, reference values and determinants in healthy subjects in. Clinical Chemistry and Laboratory Medicine (CCLM). 2007;45(7):903–911.https://www.degruyter.com/view/journals/cclm/45/7/article-p903.xml
   Google Scholar
• Godderis L, Schouteden C, Tabish A, et al. Global methylation and hydroxymethylation in DNA from blood and saliva in healthy volunteers. Biomed Res Int. 2015;2015:1–8.
   Google Scholar
• Weiner AS, Boyarskikh UA, Voronina EN, et al. Methylenetetrahydrofolate reductase C677T and methionine synthase A2756G polymorphisms influence on leukocyte genomic DNA methylation level. Gene. 2014;533(1):168–172.
   Google Scholar
• Liu J, Hesson LB, Ward RL. Liquid chromatography tandem mass spectrometry for the measurement of global DNA methylation and hydroxymethylation J Proteomics Bioinform S. 2013;2:5–10.
   Google Scholar
• Kurdyukov S, Bullock M, Methylation Analysis: DNA Choosing the right method. Biology, 2016;5(1):3. DOI:10.3390/biology5010003
   Google Scholar
• Staunstrup NH, Starnawska A, Nyegaard M, et al. Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots. Clin Epigenetics. 2016;8(1):81.
   Google Scholar
• Lisanti S, Omar WAW, Tomaszewski B, et al. Comparison of methods for quantification of global DNA methylation in human cells and tissues. PLoS One. 2013;8:79044.
   Google Scholar
• Hohos NM, Lee K, Ji L, et al. DNA cytosine hydroxymethylation levels are distinct among non-overlapping classes of peripheral blood leukocytes. J Immunol Methods. 2016;436:1–15.
   Google Scholar
• Burnham KP, Anderson DR Multimodel inference understanding AIC and BIC in model selection. Sociological methods & research. 2004;33(2):261–304. doi:10.1177/0049124104268644.
   Google Scholar
• Zerback T. Interaction Effects. Int. Encycl. Commun. Res. Methods. Wiley; 2017. 1–9. Doi:10.1002/9781118901731.iecrm0118
   Google Scholar
• Modeling Strategy Guidelines. Logist Regres. New York: Springer-Verlag. 2006;161–189. doi:10.1007/0-387-21647-2_6
   Google Scholar
• Package “nlme”. 2020.
   Google Scholar