523
Views
1
CrossRef citations to date
0
Altmetric
Research Paper

Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation

, ORCID Icon, ORCID Icon, , , , ORCID Icon, & show all
Pages 1251-1259 | Received 23 Jun 2020, Accepted 19 Nov 2020, Published online: 28 Dec 2020

References

  • Crawford DJ, Liu MY, Nabel CS, et al. Tet2 catalyzes stepwise 5-methylcytosine oxidation by an iterative and de novo mechanism. J Am Chem Soc. 2016;138(3):730–733.
  • Fenaux P, Mufti GJ, Hellstrom-Lindberg E, et al. Azacitidine prolongs overall survival compared with conventional care regimens in elderly patients with low bone marrow blast count acute myeloid leukemia. J Clin Oncol. 2010;28(4):562–569.
  • Silverman LR, Holland JF, Weinberg RS, et al. Effects of treatment with 5-azacytidine on the in vivo and in vitro hematopoiesis in patients with myelodysplastic syndromes. Leukemia. 1993;7(Suppl 1):21–29.
  • Vogler WR, Miller DS, Keller JW. 5-Azacytidine (NSC 102816): a new drug for the treatment of myeloblastic leukemia. Blood. 1976;48(3):331–337.
  • Zagonel V, Lo Re G, Marotta G, et al. 5-Aza-2ʹ-deoxycytidine (decitabine) induces trilineage response in unfavourable myelodysplastic syndromes. Leukemia. 1993;7(Suppl 1):30–35.
  • Creusot F, Acs G, Christman JK. Inhibition of DNA methyltransferase and induction of friend erythroleukemia cell differentiation by 5-azacytidine and 5-aza-2ʹ-deoxycytidine. J Biol Chem. 1982;257(4):2041–2048.
  • Jones PA, Taylor SM. Cellular differentiation, cytidine analogs and DNA methylation. Cell. 1980;20(1):85–93.
  • Jones PA, Taylor SM. Hemimethylated duplex DNAs prepared from 5-azacytidine-treated cells. Nucleic Acids Res. 1981;9(12):2933–2947.
  • Arand J, Wossidlo M, Lepikhov K, et al. Selective impairment of methylation maintenance is the major cause of DNA methylation reprogramming in the early embryo. Epigenetics Chromatin. 2015;8(1):1.
  • Laird CD, Pleasant ND, Clark AD, et al. Hairpin-bisulfite PCR: assessing epigenetic methylation patterns on complementary strands of individual DNA molecules. Proc Nat Acad Sci. 2004;101(1):204–209.
  • Miner BE, Stoger RJ, Burden AF, et al. Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR. Nucleic Acids Res. 2004;32(17):e135.
  • Chen G, Deng X. Cell synchronization by double thymidine block. Biol Protoc. 2018;8:17.
  • Peat JR, Ortega-Recalde O, Kardailsky O, et al. The elephant shark methylome reveals conservation of epigenetic regulation across jawed vertebrates. F1000Res. 2017;6:526.
  • Krueger F, Andrews SR. Bismark: a flexible aligner and methylation caller for bisulfite-seq applications. Bioinformatics. 2011;27(11):1571–1572.
  • Gordon M, El-Kalla M, Baksh S. RASSF1 polymorphisms in cancer. Mol Biol Int. 2012;(2012:365213.
  • Milani L, Lundmark A, Kiialainen A, et al. DNA methylation for subtype classification and prediction of treatment outcome in patients with childhood acute lymphoblastic leukemia. Blood. 2010;115(6):1214–1225.
  • Taylor KH, Pena-Hernandez KE, Davis JW, et al. Large-scale CpG methylation analysis identifies novel candidate genes and reveals methylation hotspots in acute lymphoblastic leukemia. Cancer Res. 2007;67(6):2617–2625.
  • Quail MA, Swerdlow H, Turner DJ. Improved protocols for the illumina genome analyzer sequencing system. Curr Protoc Hum Genet, Chapter. 2009;18:Unit 18.12.
  • Lutsik P, Feuerbach L, Arand J, et al. BiQ analyzer HT: locus-specific analysis of DNA methylation by high-throughput bisulfite sequencing. Nucleic Acids Res. 2011;39:W551–556. (Web Server issue)
  • Friedman S. The inhibition of DNA(cytosine-5)methylases by 5-azacytidine. the effect of azacytosine-containing DNA. Mol Pharmacol. 1981;19(2):314–320.
  • Santi DV, Norment A, Garrett CE. Covalent bond formation between a DNA-cytosine methyltransferase and DNA containing 5-azacytosine. Proc Natl Acad Sci U S A. 1984;81(22):6993–6997.
  • Hajek M, Votruba I, Holy A, et al. Alpha anomer of 5-aza-2ʹ-deoxycytidine down-regulates hTERT mRNA expression in human leukemia HL-60 cells. Biochem Pharmacol. 2008;75(4):965–972.
  • Öz S, Raddatz G, Rius M, et al. Quantitative determination of decitabine incorporation into DNA and its effect on mutation rates in human cancer cells. Nucleic Acids Res. 2014;42(19):e152.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.