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Review

The potential effects of Ocimum basilicum on health: a review of pharmacological and toxicological studies

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Pages 679-692 | Received 02 Feb 2018, Accepted 31 May 2018, Published online: 11 Jun 2018

References

  • Kojima G, Avgerinou C, Iliffe S, et al. Adherence to Mediterranean diet reduces incident frailty risk: systematic review and meta-analysis. J Am Geriatr Soc. 2018;66:783–788.
  • Martinez-Gonzalez MA, Guillen-Grima F, De Irala J, et al. The Mediterranean diet is associated with a reduction in premature mortality among middle-aged adults. J Nutr. 2012;142:1672–1678.
  • Bower A, Marquez S, De Mejia EG. The health benefits of selected culinary herbs and spices found in the traditional Mediterranean diet. Crit Rev Food Sci Nutr. 2016;56:2728–2746.
  • Van Den Berg SJ, Restani P, Boersma MG, et al. Levels of genotoxic and carcinogenic compounds in plant food supplements and associated risk assessment. Food Nutr Sci. 2011;2:989–1010.
  • Feighner JP. Mechanism of action of antidepressant medications. J Clin Psychiatry. 1999;60(Suppl 4): 4–11. discussion 12–13.
  • Taylor C, Fricker AD, Devi LA, et al. Mechanisms of action of antidepressants: from neurotransmitter systems to signaling pathways. Cell Signal. 2005;17:549–557.
  • Iovieno N, Dalton ED, Fava M, et al. Second-tier natural antidepressants: review and critique. J Affect Disord. 2011;130:343–357.
  • Ayuob NN, Firgany AEL, El-Mansy AA, et al. Can Ocimum basilicum relieve chronic unpredictable mild stress-induced depression in mice? Exp Mol Pathol. 2017;103:153–161.
  • Muneefa K, Doss V, Sowndarya R. Beneficial effect of hydroethanolic extract of Ocimum basilicum L on enzymic and non enzymic antioxidant in depression induced rats. J Med Plants Stud. 2017;5:185–188.
  • Zahra K, Khan MA, Iqbal F. Oral supplementation of Ocimum basilicum has the potential to improves the locomotory, exploratory, anxiolytic behavior and learning in adult male albino mice. Neurol Sci. 2015;36:73–78.
  • Rabbani M, Sajjadi SE, Vaezi A. Evaluation of anxiolytic and sedative effect of essential oil and hydroalcoholic extract of Ocimum basilicum L. and chemical composition of its essential oil. Res Pharm Sci. 2015;10:535–543.
  • Askari VR, Baradaran Rahimi V, Ghorbani A, et al. Hypnotic effect of Ocimum basilicum on pentobarbital-induced sleep in mice. Iran Red Crescent Med J. 2016;18:e24261.
  • Oliveira JS, Porto LA, Estevam CS, et al. Phytochemical screening and anticonvulsant property of Ocimum basilicum leaf essential oil. B Latinoam Caribe Pl. 2009;8:195-202.
  • Chen SJ, Wang MH, Chen IJ. Antiplatelet and calcium inhibitory properties of eugenol and sodium eugenol acetate. Gen Pharmacol. 1996;27:629–633.
  • Tognolini M, Barocelli E, Ballabeni V, et al. Comparative screening of plant essential oils: phenylpropanoid moiety as basic core for antiplatelet activity. Life Sci. 2006;78:1419–1432.
  • Amrani S, Harnafi H, Bouanani Nel H, et al. Hypolipidaemic activity of aqueous Ocimum basilicum extract in acute hyperlipidaemia induced by triton WR-1339 in rats and its antioxidant property. Phytother Res. 2006;20:1040–1045.
  • Amrani S, Harnafi H, Gadi D, et al. Vasorelaxant and anti-platelet aggregation effects of aqueous Ocimum basilicum extract. J Ethnopharmacol. 2009;125:157–162.
  • Irondi EA, Agboola SO, Oboh G, et al. Inhibitory effect of leaves extracts of Ocimum basilicum and Ocimum gratissimum on two key enzymes involved in obesity and hypertension in vitro. J Intercult Ethnopharmacol. 2016;5:396–402.
  • Amirdivani S, Baba AS. Changes in yogurt fermentation characteristics, and antioxidant potential and in vitro inhibition of angiotensin-1 converting enzyme upon the inclusion of peppermint, dill and basil. LWT-Food Sci Technol. 2011;44:1458–1464.
  • Umar A, Imam G, Yimin W, et al. Antihypertensive effects of Ocimum basilicum L. (OBL) on blood pressure in renovascular hypertensive rats. Hypertens Res. 2010;33:727–730.
  • Fathiazad F, Matlobi A, Khorrami A, et al. Phytochemical screening and evaluation of cardioprotective activity of ethanolic extract of Ocimum basilicum L. (basil) against isoproterenol induced myocardial infarction in rats. Daru. 2012;20:87.
  • Umar A, Zhou W, Abdusalam E, et al. Effect of Ocimum basilicum L. on cyclo-oxygenase isoforms and prostaglandins involved in thrombosis. J Ethnopharmacol. 2014;152:151–155.
  • Kuerban A, Almulaiky YQ, Sheikh RA, et al. In vivo antithrombotic activity of ethanolic extract from Ocimum basilicum L. Annu Res Rev Biol. 2017;20:1–6.
  • Boskabady MH, Kiani S, Haghiri B. Relaxant effects of Ocimum basilicum on guinea pig tracheal chains and its possible mechanism (s). Daru. 2005;13:28–33.
  • Noumi E. Treating asthma with medicinal plants. an ethnomedicinal case study from Baré‐Bakem, Nkongsamba Region, Cameroon. Syllabus Rev. 2009;1:10–15.
  • Nath K, Deka P, Borkataki S, et al. Traditional remedies of respiratory disorders from Assam, India. Pleione. 2008;2:211–216.
  • Lemos ICS, Delmondes GA, Dos Santos ADF, et al. Ethnobiological survey of plants and animals used for the treatment of acute respiratory infections in children of a traditional community in the municipality of Barbalha, Ceara, Brazil. Afr J Tradit Complement Altern Med. 2016;13:166–175.
  • Gemechu A, Giday M, Worku A, et al. In vitro anti-mycobacterial activity of selected medicinal plants against Mycobacterium tuberculosis and Mycobacterium bovis strains. BMC Complement Altern Med. 2013;13:291.
  • Janbaz KH, Hamid I, Gilani A-U-H, et al. Spasmolytic, bronchodilator and vasodilator activities of aqueous-methanolic extract of Ocimum basilicum. Int J Agric Biol. 2014;16:321-327.
  • Chatterjee S, Khunti K, Davies MJ. Type 2 diabetes. Lancet. 2017;389:2239–2251.
  • Aragno M, Parola S, Tamagno E, et al. Oxidative derangement in rat synaptosomes induced by hyperglycaemia: restorative effect of dehydroepiandrosterone treatment. Biochem Pharmacol. 2000;60:389–395.
  • Habeck M. Diabetes treatments get sweet help from nature. Nat Med. 2003;9:1228.
  • El-Beshbishy H, Bahashwan S. Hypoglycemic effect of basil (Ocimum basilicum) aqueous extract is mediated through inhibition of alpha-glucosidase and alpha-amylase activities: an in vitro study. Toxicol Ind Health. 2012;28:42–50.
  • Kadan S, Saad B, Sasson Y, et al. In vitro evaluation of anti-diabetic activity and cytotoxicity of chemically analysed Ocimum basilicum extracts. Food Chem. 2016;196:1066–1074.
  • Ezeani C, Ezenyi I, Okoye T, et al. Ocimum basilicum extract exhibits antidiabetic effects via inhibition of hepatic glucose mobilization and carbohydrate metabolizing enzymes. J Intercult Ethnopharmacol. 2017;6:22–28.
  • Antora RA, Salleh RM. Antihyperglycemic effect of Ocimum plants: a short review. Asian Pac J Trop Med. 2017;7:755–759.
  • Singh P, Jayaramaiah RH, Agawane SB, et al. Potential dual role of eugenol in inhibiting advanced glycation end products in diabetes: proteomic and mechanistic insights. Sci Rep. 2016;6:18798.
  • Wolff SP. Diabetes mellitus and free radicals. Free radicals transition metals and oxidative stress in the aetiology of diabetes mellitus and complications. Br Med Bull. 1993;49:642–652.
  • Ugwu M, Umar I, Utu-Baku A, et al. Antioxidant status and organ function in streptozotocin-induced diabetic rats treated with aqueous, methanolic and petroleum ether extracts of Ocimum basilicum leaf. J App Pharm Sci. 2013;3:S75–S79.
  • Singh S. Evaluation of gastric anti-ulcer activity of fixed oil of Ocimum basilicum Linn. and its possible mechanism of action. Indian J Exp Biol. 1999;37:253–257.
  • Rafieian KM, Hosseini-Asl K. Effects of Ocimum Basilicum on functional Dyspepsia: a double-blind placebo-controlled study. Iran J Med Sci. 2015;30:134–137.
  • Rashidian A, Roohi P, Mehrzadi S, et al. Protective effect of Ocimum basilicum essential oil against acetic acid-induced colitis in rats. J Evid Based Complementary Altern Med. 2016;21:NP36–42.
  • El-Sayed NM. Evaluation the in vitro effects of ethanol extracts of Ocimum basilicum (sweet basil) and Thymus vulgaris (thyme) for anti-Blastocystis hominis activity. Egypt J Med Sci. 2009;30:1229–1243.
  • El-Badry AA, Al-Ali KH, El-Badry YA. Activity of Mentha longifolia and Ocimum basilicum against Entamoeba histolytica and Giardia duodenalis. Sci Parasitol. 2010;11:109–117.
  • Castillo-Juarez I, Gonzalez V, Jaime-Aguilar H, et al. Anti-Helicobacter pylori activity of plants used in Mexican traditional medicine for gastrointestinal disorders. J Ethnopharmacol. 2009;122:402–405.
  • Nathan C. Neutrophils and immunity: challenges and opportunities. Nat Rev Immunol. 2006;6:173–182.
  • Carson JL, Willett LR. Toxicity of nonsteroidal anti-inflammatory drugs. An overview of the epidemiological evidence. Drugs. 1993;46(Suppl 1):243–248.
  • Maroon JC, Bost JW, Maroon A. Natural anti-inflammatory agents for pain relief. Surg Neurol Int. 2010;1:80.
  • Mueller M, Hobiger S, Jungbauer A. Anti-inflammatory activity of extracts from fruits, herbs and spices. Food Chem. 2010;122:987–996.
  • Tsai PJ, Tsai TH, Yu CH, et al. Evaluation of NO-suppressing activity of several Mediterranean culinary spices. Food Chem Toxicol. 2007;45:440–447.
  • Benedec D, Parvu AE, Oniga I, et al. Effects of Ocimum basilicum L. extract on experimental acute inflammation. Rev Med Chir Soc Med Nat Iasi. 2007;111:1065–1069.
  • Rodrigues LB, Oliveira Brito Pereira Bezerra Martins A, Cesario FR, et al. Anti-inflammatory and antiedematogenic activity of the Ocimum basilicum essential oil and its main compound estragole: in vivo mouse models. Chem Biol Interact. 2016;257:14–25.
  • Loi M, Maxia L, Maxia A. Ethnobotanical comparison between the villages of Escolca and Lotzorai (Sardinia, Italy). J Herbs Spices Med Plants. 2005;11:67–84.
  • Padal S, Sri BS, Raju J. Ethno-medicine used against fever among the tribes of Visakhapatnam district, Andhra Pradesh, India. Int J Ethnobiol Ethnomed. 2014;1:1–5.
  • Venancio AM, Onofre AS, Lira AF, et al. Chemical composition, acute toxicity, and antinociceptive activity of the essential oil of a plant breeding cultivar of basil (Ocimum basilicum L.). Planta Med. 2011;77:825–829.
  • Nascimento SS, Araujo AA, Brito RG, et al. Cyclodextrin-complexed Ocimum basilicum leaves essential oil increases Fos protein expression in the central nervous system and produce an antihyperalgesic effect in animal models for fibromyalgia. Int J Mol Sci. 2014;16:547–563.
  • Shirazi MT, Gholami H, Kavoosi G, et al. Chemical composition, antioxidant, antimicrobial and cytotoxic activities of Tagetes minuta and Ocimum basilicum essential oils. Food Sci Nutr. 2014;2:146–155.
  • Stefan M, Zamfirache M, Padurariu C, et al. The composition and antibacterial activity of essential oils in three Ocimum species growing in Romania. Open Life Sciences. 2013;8:600–608.
  • Kadhim MJ, Sosa AA, Hameed IH. Evaluation of anti-bacterial activity and bioactive chemical analysis of Ocimum basilicum using Fourier transform infrared (FT-IR) and gas chromatography-mass spectrometry (GC-MS) techniques. J Pharmacognosy Phytother. 2016;8:127–146.
  • Opalchenova G, Obreshkova D. Comparative studies on the activity of basil–an essential oil from Ocimum basilicum L.–against multidrug resistant clinical isolates of the genera Staphylococcus, Enterococcus and Pseudomonas by using different test methods. J Microbiol Methods. 2003;54:105–110.
  • Wiwattanarattanabut K, Choonharuangdej S, Srithavaj T. In vitro anti-cariogenic plaque effects of essential oils extracted from culinary herbs. J Clin Diagn Res. 2017;11:DC30–DC35.
  • Kaya I, Yigit N, Benli M. Antimicrobial activity of various extracts of Ocimum basilicum L. and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy. Afr J Tradit Complement Altern Med. 2008;5:363–369.
  • Siddiqui BS, Bhatti HA, Begum S, et al. Evaluation of the antimycobacterium activity of the constituents from Ocimum basilicum against Mycobacterium tuberculosis. J Ethnopharmacol. 2012;144:220–222.
  • Shukla R, Singh P, Prakash B, et al. Antifungal, aflatoxin inhibitory and free radical‐scavenging activities of some medicinal plants extracts. J Food Qual. 2012;35:182–189.
  • El-Soud NH, Deabes M, El-Kassem LA, et al. Chemical composition and antifungal activity of Ocimum basilicum L. essential oil. Open Access Maced J Med Sci. 2015;3:374–379.
  • Ezz Eldin HM, Badawy AF. In vitro anti-Trichomonas vaginalis activity of Pistacia lentiscus mastic and Ocimum basilicum essential oil. J Parasit Dis. 2015;39:465–473.
  • Ntonga PA, Baldovini N, Mouray E, et al. Activity of Ocimum basilicum, Ocimum canum, and Cymbopogon citratus essential oils against Plasmodium falciparum and mature-stage larvae of Anopheles funestus s.s. Parasite. 2014;21:33.
  • Inbaneson SJ, Sundaram R, Suganthi P. In vitro antiplasmodial effect of ethanolic extracts of traditional medicinal plant Ocimum species against Plasmodium falciparum. Asian Pac J Trop Med. 2012;5:103–106.
  • Chavan SR, Nikam ST. Mosquito larvicidal activity of Ocimum basilicum Linn. Indian J Med Res. 1982;75:220–222.
  • Chiang LC, Ng LT, Cheng PW, et al. Antiviral activities of extracts and selected pure constituents of Ocimum basilicum. Clin Exp Pharmacol Physiol. 2005;32:811–816.
  • Siegel R, Ward E, Brawley O, et al. Cancer statistics, 2011: the impact of eliminating socioeconomic and racial disparities on premature cancer deaths. CA Cancer J Clin. 2011;61:212–236.
  • Zamora-Ros R, Knaze V, Rothwell JA, et al. Dietary polyphenol intake in Europe: the European Prospective Investigation into Cancer and Nutrition (EPIC) study. Eur J Nutr. 2016;55:1359–1375.
  • Chalchat JC, Ozcan MM. Comparative essential oil composition of flowers, leaves and stems of basil (Ocimum basilicum L.) used as herb. Food Chem. 2008;110:501–503.
  • Sestili P, Fimognari C. Cytotoxic and antitumor activity of sulforaphane: the role of reactive oxygen species. Biomed Res Int. 2015;2015:402386.
  • Elansary HO, Mahmoud EA. In vitro antioxidant and antiproliferative activities of six international basil cultivars. Nat Prod Res. 2015;29:2149–2154.
  • Abirami SG, Nirmala P. A comparative–in vitro study of anticancer effect of Mentha piperita, Ocimum basilicum and Coleus aromaticus against human laryngeal epidermoid carcinoma (HEP-2) cell lines. J Med Plants Stud. 2014;2:6-9.
  • Jaganathan SK, Supriyanto E. Antiproliferative and molecular mechanism of eugenol-induced apoptosis in cancer cells. Molecules. 2012;17:6290–6304.
  • Hussain A, Priyani A, Sadrieh L, et al. Concurrent sulforaphane and eugenol induces differential effects on human cervical cancer cells. Integr Cancer Ther. 2012;11:154–165.
  • Jaganathan SK, Mondhe D, Wani ZA, et al. Effect of honey and eugenol on Ehrlich ascites and solid carcinoma. J Biomed Biotechnol. 2010;2010:989163.
  • Qamar KA, Dar A, Siddiqui BS, et al. Anticancer activity of Ocimum basilicum and the effect of ursolic acid on the cytoskeleton of MCF-7 human breast cancer cells. Lett Drug Des Discov. 2010;7:726–736.
  • Ravizza R, Gariboldi MB, Molteni R, et al. Linalool, a plant-derived monoterpene alcohol, reverses doxorubicin resistance in human breast adenocarcinoma cells. Oncol Rep. 2008;20:625–630.
  • Monga J, Sharma M, Tailor N, et al. Antimelanoma and radioprotective activity of alcoholic aqueous extract of different species of Ocimum in C(57)BL mice. Pharm Biol. 2011;49:428–436.
  • Dasgupta T, Rao AR, Yadava PK. Chemomodulatory efficacy of basil leaf (Ocimum basilicum) on drug metabolizing and antioxidant enzymes, and on carcinogen-induced skin and forestomach papillomagenesis. Phytomedicine. 2004;11:139–151.
  • Gajula D, Verghese M, Boateng I, et al. Basil (Ocimum basilicum and Ocimum tenuiflorum) reduces azoxymethane induced colon tumors in fisher 344 male rats. Res J Phytochem. 2010;4:136–145.
  • Sukumaran K, Unnikrishnan MC, Kuttan R. Inhibition of tumour promotion in mice by eugenol. Indian J Physiol Pharmacol. 1994;38:306–308.
  • Gulcin I, Elmastas M, Aboul-Enein HY. Determination of antioxidant and radical scavenging activity of Basil (Ocimum basilicum L. Family Lamiaceae) assayed by different methodologies. Phytother Res. 2007;21:354–361.
  • Kaurinovic B, Popovic M, Vlaisavljevic S, et al. Antioxidant capacity of Ocimum basilicum L. and Origanum vulgare L. extracts. . Molecules. 2011;16:7401–7414.
  • Aydemir T, Becerik S. Phenolic content and antioxidant activity of different extracts from Ocimum basilicum, Apium graveolens and Lepidium sativum seeds. J Food Biochem. 2011;35:62–79.
  • Zakaria Z, Aziz R, Lachimanan YL, et al. Antioxidant activity of Coleus blumei, Orthosiphon stamineus, Ocimum basilicum and Mentha arvensis from Lamiaceae family. Int J Nat Eng Sci. 2008;2:93–95.
  • Flanigan PM, Niemeyer ED. Effect of cultivar on phenolic levels, anthocyanin composition, and antioxidant properties in purple basil (Ocimum basilicum L.). Food Chem. 2014;164:518–526.
  • Javanmardi J, Stushnoff C, Locke E, et al. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem. 2003;83:547–550.
  • Fimognari C, Sestili P, Lenzi M, et al. Protective effect of creatine against RNA damage. Mutat Res. 2009;670:59–67.
  • Jayasinghe C, Gotoh N, Aoki T, et al. Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L.). J Agric Food Chem. 2003;51:4442–4449.
  • Sakr SA, Al-Amoudi WM. Effect of leave extract of Ocimum basilicum on deltamethrin induced nephrotoxicity and oxidative stress in albino rats. J App Pharm Sci. 2012;2:22–27.
  • Solanki R, Mathur V, Purohit SK, et al. Evaluation of wound healing activity of ethanolic extract of Ocimum basilicum and Aegle marmelos leaves in male albino rats. Int J Drug Res Technol. 2017;2:4.
  • Jadoon S, Karim S, Bin Asad MH, et al. Anti-aging potential of phytoextract loaded-pharmaceutical creams for human skin cell longetivity. Oxid Med Cell Longev. 2015;2015:709628.
  • Rasul A, Akhtar N. Formulation and in vivo evaluation for anti-aging effects of an emulsion containing basil extract using non-invasive biophysical techniques. Daru. 2011;19:344–350.
  • Free radical scavenging and tyrosinase inhibition activity of aromatic volatile oil from Thai medicinal plants for cosmetic uses. In: Manosroi A, Manosroi J, editors . III WOCMAP Congress on medicinal and aromatic plants-volume 6: traditional medicine and nutraceuticals 680. Acta Hortic (ISHS), Chiang Mai, Thailand; 2005.
  • Fang JY, Leu YL, Hwang TL, et al. Essential oils from sweet basil (Ocimum basilicum) as novel enhancers to accelerate transdermal drug delivery. Biol Pharm Bull. 2004;27:1819–1825.
  • Idu M, Umweni A, Odaro T, et al. Ethnobotanical plants used for oral healthcare among the Esan Tribe of Edo State, Nigeria. Ethnobot Leaflets. 2009;2009:15.
  • Devi AD, Devi OI, Singh TC, et al. A study of aromatic plant species especially in Thoubal district, Manipur, Northeast India. Int J Sci Res Pub. 2014;4:1–12.
  • Ahmed HM. Ethnopharmacobotanical study on the medicinal plants used by herbalists in Sulaymaniyah Province, Kurdistan, Iraq. J Ethnobiol Ethnomed. 2016;12:8.
  • Parra AL, Yhebra RS, Sardiñas IG, et al. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts. Phytomedicine. 2001;8:395–400.
  • Khan I, Ahmad K, Khalil AT, et al. Evaluation of antileishmanial, antibacterial and brine shrimp cytotoxic potential of crude methanolic extract of herb Ocimum basilicum (Lamiacea). J Tradit Chin Med. 2015;35:316–322.
  • Gadir S. Assessment of bioactivity of some Sudanese medicinal plants using brine shrimp (Artemia salina) lethality assay. J Chem Pharm Res. 2012;4:4148–5145.
  • Rasekh HR, Hosseinzadeh L, Mehri S, et al. Safety assessment of Ocimum basilicum hydroalcoholic extract in Wistar rats: acute and subchronic toxicity studies. Iran J Basic Med Sci. 2012;15:645–653.
  • Fandohan P, Gnonlonfin B, Laleye A, et al. Toxicity and gastric tolerance of essential oils from Cymbopogon citratus, Ocimum gratissimum and Ocimum basilicum in Wistar rats. Food Chem Toxicol. 2008;46:2493–2497.
  • EFSA. Scientific Opinion on the substantiation of health claims related to Ocimum basilicum L. and improvement of diuretic function (ID 2314, 3465) pursuant to Article 13 (1) of Regulation (EC) No 1924/2006. EFSA J. 2009;7:1298.
  • Pansang S, Maphanta S, Tuntijarukorn P, et al. Skin irritation test of a microemulsion containing essential oil isolated from Ocimum basilicum. Sci Asia. 2010;36:355–358.
  • EFSA. Compendium of botanicals that have been reported to contain toxic, addictive, psychotropic or other substances of concern on request of EFSA. EFSA J. 2009;9:1–100.
  • Miller EC, Swanson AB, Phillips DH, et al. Structure-activity studies of the carcinogenicities in the mouse and rat of some naturally occurring and synthetic alkenylbenzene derivatives related to safrole and estragole. Cancer Res. 1983;43:1124–1134.
  • National Toxicology Program. NTP toxicology and carcinogenesis studies of methyleugenol (CAS NO. 93–15–2) in F344/N rats and B6C3F1 mice (Gavage Studies). Natl Toxicol Program Tech Rep Ser. 2000;491:1.
  • Smith RL, Adams TB, Doull J, et al. Safety assessment of allylalkoxybenzene derivatives used as flavouring substances - methyl eugenol and estragole. Food Chem Toxicol. 2002;40:851–870.
  • Al-Malahmeh AJ, Al-Ajlouni AM, Wesseling S, et al. Determination and risk assessment of naturally occurring genotoxic and carcinogenic alkenylbenzenes in basil-containing sauce of pesto. Toxicol Reports. 2017;4:1–8.
  • EFSA. Opinion of the Scientific Committee on a request from EFSA related to a harmonised approach for risk assessment of substances which are both genotoxic and carcinogenic. EFSA J. 2005;282:1–31.
  • Miele M, Dondero R, Ciarallo G, et al. Methyleugenol in Ocimum basilicum LCv. genovese gigante. J Agric Food Chem. 2001;49:517–521.
  • Jeurissen SM, Punt A, Delatour T, et al. Basil extract inhibits the sulfotransferase mediated formation of DNA adducts of the procarcinogen 1ʹ-hydroxyestragole by rat and human liver S9 homogenates and in HepG2 human hepatoma cells. Food Chem Toxicol. 2008;46:2296–2302.
  • Alhusainy W, Paini A, Punt A, et al. Identification of nevadensin as an important herb-based constituent inhibiting estragole bioactivation and physiology-based biokinetic modeling of its possible in vivo effect. Toxicol Appl Pharmacol. 2010;245:179–190.
  • Alhusainy W, Williams GM, Jeffrey AM, et al. The natural basil flavonoid nevadensin protects against a methyleugenol-induced marker of hepatocarcinogenicity in male F344 rat. Food Chem Toxicol. 2014;74:28–34.
  • Van Den Berg SJ, Klaus V, Alhusainy W, et al. Matrix-derived combination effect and risk assessment for estragole from basil-containing plant food supplements (PFS). Food Chem Toxicol. 2013;62:32–40.
  • Zheljazkov VD, Callahan A, Cantrell CL. Yield and oil composition of 38 basil (Ocimum basilicum L.) accessions grown in Mississippi. J Agric Food Chem. 2008;56:241–245.
  • Vlase L, Benedec D, Hanganu D, et al. Evaluation of antioxidant and antimicrobial activities and phenolic profile for Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys. Molecules. 2014;19:5490–5507.
  • Da Silva LA, Pezzini BR, Soares L. Spectrophotometric determination of the total flavonoid content in Ocimum basilicum L. (Lamiaceae) leaves. Pharmacogn Mag. 2015;11:96–101.
  • Lee J, Scagel CF. Chicoric acid found in basil (Ocimum basilicum L.). leaves. Food Chem. 2009;115:650–656.
  • Srivastava S, Cahill DM, Conlan XA, et al. A novel in vitro whole plant system for analysis of polyphenolics and their antioxidant potential in cultivars of Ocimum basilicum. J Agric Food Chem. 2014;62:10064–10075.
  • Da Costa AS, Arrigoni-Blank Mde F, De Carvalho Filho JL, et al. Chemical diversity in basil (Ocimum sp.) germplasm. Scientific World Journal. 2015;2015:352638.
  • Ademiluyi AO, Oyeleye SI, Oboh G. Biological activities, antioxidant properties and phytoconstituents of essential oil from sweet basil (Ocimum basilicum L.). leaves. Comp Clin Path. 2016;25:169–176.
  • Kale S, Sonawane A, Ansari A, et al. Formulation and in-vitro determination of sun protection factor of Ocimum basilicum, Linn. leaf oils sunscreen cream. Int J Pharm Pharm Sci. 2010;2:147–149.
  • Lee S-J, Umano K, Shibamoto T, et al. Identification of volatile components in basil (Ocimum basilicum L.) and thyme leaves (Thymus vulgaris L.) and their antioxidant properties. Food Chem. 2005;91:131–137.
  • Fischer R, Nitzan N, Chaimovitsh D, et al. Variation in essential oil composition within individual leaves of sweet basil (Ocimum basilicum L.) is more affected by leaf position than by leaf age. J Agric Food Chem. 2011;59:4913–4922.

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