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Articles

Graphene-based materials do not impair physiology, gene expression and growth dynamics of the aeroterrestrial microalga Trebouxia gelatinosa

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Pages 492-509 | Received 09 Jul 2018, Accepted 13 Dec 2018, Published online: 26 Jun 2019

References

  • Ahmadjian, V. 1973. Methods of isolation and culturing lichen symbionts and thalli. In: V. Ahmadjian and M.E. Hale, eds. The lichens. New York, NY: Academic Press, 653–660.
  • Amirov, R. R., J. Shayimova, Z. Nasirova, and A. M. Dimiev. 2017. “Chemistry of Graphene Oxide. Reactions with Transition Metal Cations.” Carbon 116: 356–365. doi:10.1016/j.carbon.2017.01.095.
  • ASTM (American Society for Testing and Materials). 1985. Zeta Potential of Colloids in Water and Waste Water Standard D. 4187–92. West Conshohocken: ASTM.
  • Archibald, P. A. 1975. “Trebouxia de Pulmaly (Chlorophyceae, Chlorococcales) and Pseudotrebouxia Gen. nov. (Chlorophyceae, Chlorosarcinales).” Phycologia 14 (3): 125–137. doi:10.2216/i0031-8884-14-3-125.1.
  • Banchi, E., F. Candotto Carniel, A. Montagner, F. Petruzzellis, G. Pichler, V. Giarola, D. Bartels, A. Pallavicini, and M. Tretiach. 2018. “Relation between Water Status and Desiccation-affected Genes in the Lichen Photobiont Trebouxia gelatinosa.” Plant Physiology and Biochemistry 129 (5): 189–197. doi:10.1016/j.plaphy.2018.06.004.
  • Bertuzzi, S., L. Gustavs, G. Pandolfini, and M. Tretiach. 2017. “Heat Shock Treatments for the Control of Lithobionts: A Case Study with Epilithic Green Microalgae.” International Biodeterioration & Biodegradation 123: 236–243. doi:10.1016/j.ibiod.2017.06.023.
  • Bianco, A., and M. Prato. 2015. “Safety Concerns on Graphene and 2D Materials: A Flagship Perspective.” 2D Materials 2.3: 1–3. doi:10.1088/2053-1583/2/3/030201.
  • Bianco, A., Cheng, H. M. Enoki T., Y. Gogotsi, R. H. Hurt, N. Koratkar, T. Kyotani, M. Monthioux, et al. 2013. “All in the Graphene Family – a Recommended Nomenclature for Two-dimensional Carbon Materials.” Carbon 65: 1–6. doi:10.1016/j.carbon.2013.08.038.
  • Bondarenko, O., K. Juganson, A. Ivask, K. Kasemets, M. Mortimer, and A. Kahru. 2013. “Toxicity of Ag, CuO and ZnO Nanoparticles to Selected Environmentally Relevant Test Organisms and Mammalian Cells in Vitro: a Critical Review.” Archives of Toxicology 87 (7): 1181–200. doi:10.1007/s00204-013-1079-4.
  • Bramini, M., S. Sacchetti, A. Armirotti, A. Rocchi, E. Vázquez, V. León Castellanos, T. Bandiera, F. Cesca, and F. Benfenati. 2016. “Graphene Oxide Nanosheets Disrupt Lipid Composition, Ca2+ homeostasis, and Synaptic Transmission in Primary Cortical Neurons.” ACS Nano 10 (7): 7154–7171. doi:10.1021/acsnano.6b03438.
  • Candotto Carniel, F., M. Gerdol, A. Montagner, E. Banchi, G. De Moro, C. Manfrin, L. Muggia, A. Pallavicini, and M. Tretiach. 2016. “New Features of Desiccation Tolerance in the Lichen Photobiont Trebouxia gelatinosa Are Revealed by a Transcriptomic Approach.” Plant Molecular Biology 91 (3): 319–339. doi:10.1007/s11103-016-0468-5.
  • Candotto Carniel, F., D. Zanelli, S. Bertuzzi, and M. Tretiach. 2015. “Desiccation Tolerance and Lichenization: A Case Study with the Aeroterrestrial Microalga Trebouxia sp. (Chlorophyta).” Planta 242 (2): 493–505. doi:10.1007/s00425-015-2319-z.
  • Candotto Carniel, F., D. Gorelli, F. Flahaut, L. Fortuna, C. Del Casino, G. Cai, M. Nepi, M. Prato, and M. Tretiach. 2018. “Graphene Oxide Impairs the Pollen Performance of Nicotiana tabacum and Corylus avellana Suggesting Potential Negative Effects on the Sexual Reproduction of Seed Plants.” Environmental Science: Nano 5: 1608–1617. doi:10.1039/c8en00052b.
  • Casano, L. M., M. R. Braga, R. Álvarez, E. M. del Campo, and E. Barreno. 2015. “Differences in the Cell Walls and Extracellular Polymers of the Two Trebouxia Microalgae Coexisting in the Lichen Ramalina farinacea Are Consistent with Their Distinct Capacity to Immobilize Extracellular Pb.” Plant Science 236: 195–204. doi:10.1016/j.plantsci.2015.04.003.
  • Ciriminna, R., N. Zhang, M. Q. Yang, F. Meneguzzo, Y. J. Xu, and M. Pagliaro. 2015. “Commercialization of Graphene-based Technologies: A Critical Insight.” Chemical Communications 51 (33): 7090–7095. doi:10.1039/C5CC01411E.
  • Dahmen, H., T. Staub, and F. J. Schwinn. 1983. “Technique for Long-term Preservation of Phytopathogenic Fungi in Liquid Nitrogen.” Phytopathology 73 (2): 241–246. doi:10.1094/Phyto-73-241.
  • Del Hoyo, A., R. Álvarez, E. M. del Campo, F. Gasulla, E. Barreno, and L. M. Casano. 2011. “Oxidative Stress Induces Distinct Physiological Responses in the Two Trebouxia Phycobionts of the Lichen Ramalina farinacea.” Annals of Botany 107 (1): 109–118. doi:10.1093/aob/mcq206.
  • Doudrick, K., P. Herckes, and P. Westerhoff. 2012. “Detection of Carbon Nanotubes in Environmental Matrices Using Programmed Thermal Analysis.” Environmental Science & Technology 46 (22): 12246–12253. doi:10.1021/es300804f.
  • Du, S., P. Zhang, R. Zhang, Q. Lu, L. Liu, X. Bao, and H. Liu. 2016. “Reduced Graphene Oxide Induces Cytotoxicity and Inhibits Photosynthetic Performance of the Green Alga Scenedesmus obliquus.” Chemosphere 164: 499–507. doi:10.1016/j.chemosphere.2016.08.138.
  • Fadeel, B., Bussy, C. Merino S., E. Vázquez, E. Flahaut, F. Mouchet, L. Evariste, et al. 2018. “Safety Assessment of Graphene-based Materials: focus on Human Health and the Environment.” ACS Nano 12 (11): 10582–10620. doi:10.1021/acsnano.8b04758.
  • Ferrari, A. C., F. Bonaccorso, V. Fal'ko, K. S. Novoselov, S. Roche, P. Bøggild, S. Borini., et al. 2015. “Science and Technology Roadmap for Graphene, Related Two-Dimensional Crystals, and Hybrid Systems.” Nanoscale 7 (11): 4598–4810. doi:10.1039/C4NR01600A.
  • Ferrari, A. C., Meyer, J. C. Scardaci V., C. Casiraghi, M. Lazzeri, F. Mauri, S. Piscanec, D. Jiang, et al. 2006. “Raman Spectrum of Graphene and Graphene Layers.” Physical Review Letters 97 (18): 187401. doi:10.1103/PhysRevLett.97.187401.
  • Freystein, K., and W. Reisser. 2010. Green biofilms on tree barks: more than just algae. In: J. Seckbach and M. Grube, eds. Symbioses and Stress: Joint Ventures in Biology. Dordrecht, D; Springer, 557–573.
  • Friedl, T. 1989. “Comparative Ultrastructure of Pyrenoids in Trebouxia (Microthamniales, Chlorophyta).” Plant Systematics and Evolution 164 (1-4): 145–159. doi:10.1007/BF00940435.
  • Garacci, M., M. Barret, F. Mouchet, C. Sarrieu, P. Lonchambon, E. Flahaut, L. Gauthier, J. Silvestre, and E. Pinelli. 2017. “Few Layer Graphene Sticking by Biofilm of Freshwater Diatom Nitzschia palea as a Mitigation to Its Ecotoxicity.” Carbon 113: 139–150. doi:10.1016/j.carbon.2016.11.033.
  • Ghaffarzadeh, K. 2016. Graphene 2D Materials and Carbon Nanotubes: Markets Technologies and Opportunities 2016–2026 [online]. Available from: http://wwwidtechexcom/research/reports/graphene–2d–materials–and–carbon–nanotubes–markets–technologies–and–opportunities–2015–2025–000440asp?viewopt=desc [Accessed March 2018].
  • Gogotsi, Y. 2015. “Not Just Graphene: the Wonderful World of Carbon and Related Nanomaterials.” MRS Bulletin 40 (12): 1110–1120. doi:10.1557/mrs.2015.272.
  • Gorbushina, A. A., and W. J. Broughton. 2009. “Microbiology of the Atmosphere-rock Interface: how Biological Interactions and Physical Stresses Modulate a Sophisticated Microbial Ecosystem.” Annual Review of Microbiology 63 (1): 431–450. doi:10.1146/annurev.micro.091208.073349.
  • Haeder, D. P., V. E. Villafane, and E. W. Helbling. 2014. “Productivity of Aquatic Primary Producers under Global Climate Change.” Photochemical & Photobiological Sciences 13: 1370–1392. doi:10.1039/C3PP50418B.
  • Haniff Wahid, M., E. Eroglu, X. Chen, S. M. Smith, and C. L. Raston. 2013. “Entrapment of Chlorella vulgaris Cells within Graphene Oxide Layers.” RSC Advances 3 (22): 8180–8183. doi:10.1039/c3ra40605a.
  • Hauck, M., A. Paul, C. Mulack, E. Fritz, and M. Runge. 2002. “Effects of Manganese on the Viability of Vegetative Diaspores of the Epiphytic Lichen Hypogymnia physodes.” Environmental and Experimental Botany 47 (2): 127–142. doi:10.1016/S0098-8472(01)00121-6.
  • Hazeem, L. J., M. Bououdina, E. Dewailly, C. Slomianny, A. Barras, Y. Coffinier, S. Szunerits, and R. Boukherroub. 2017. “Toxicity Effect of Graphene Oxide on Growth and Photosynthetic Pigment of the Marine Alga Picochlorum sp. during Different Growth Stages.” Environmental Science and Pollution Research 24 (4): 4144–4152. doi:10.1007/s11356-016-8174-z.
  • Holzinger, A., and U. Karsten. 2013. “Desiccation Stress and Tolerance in Green Algae: Consequences for Ultrastructure Physiological and Molecular Mechanisms.” Frontiers in Plant Sciences 4: 327. doi: doi:10.3389/fpls.2013.00327.
  • Horton, A. A., A. Walton, D. J. Spurgeon, E. Lahive, and C. Svendsen. 2017. “Microplastics in Freshwater and Terrestrial Environments: Evaluating the Current Understanding to Identify the Knowledge Gaps and Future Research Priorities.” Science of the Total Environment 586: 127–141. doi:10.1016/j.scitotenv.2017.01.190.
  • Hu, C., Q. Wang, H. Zhao, L. Wang, S. Guo, and X. Li. 2015. “Ecotoxicological Effects of Graphene Oxide on the Protozoan Euglena gracilis.” Chemosphere 128: 184–190. doi:10.1016/j.chemosphere.2015.01.040.
  • Hu, X., and Q. Zhou. 2013. “Health and Ecosystem Risks of Graphene.” Chemical Reviews 113 (5): 3815–3385. doi:10.1021/cr300045n.
  • Hu, X., K. Lu, L. Mu, J. Kang, and Q. Zhou. 2014. “Interactions Between Graphene Oxide and Plant Cells: Regulation of Cell Morphology, Uptake, Organelle Damage, Oxidative Effects and Metabolic Disorders.” Carbon 80: 665–676. doi:10.1016/j.carbon.2014.09.010.
  • Huang, J., C. Zong, H. Shen, M. Liu, B. Chen, B. Ren, and Z. Zhang. 2012. “Mechanism of Cellular Uptake of Graphene Oxide Studied by Surface-enhanced Raman Spectroscopy.” Small 8 (16): 2577–2584. doi:10.1002/smll.201102743.
  • Jastrzębska, A. M., and A. R. Olszyna. 2015. “The Ecotoxicity of Graphene Family Materials: Current Status, Knowledge Gaps and Future Needs.” Journal of Nanoparticles Research 17: 40. doi:10.1007/s11051-014-2817-0.
  • Karsten, U., R. Schumann, and A. Mostaert. 2007. Aeroterrestrial algae growing on man-made surfaces. In: J. Seckbach eds. Algae and Cyanobacteria in Extreme Environments. Dordrecht, D; Springer, 583–597.
  • Krishnamoorthy, K.,. M. Veerapandian, K. Yun, and S. J. Kim. 2013. “The Chemical and Structural Analysis of Graphene Oxide with Different Degrees of Oxidation.” Carbon 53: 38–49. doi:10.1016/j.carbon.2012.10.013.
  • König, J., and E. Peveling. 1984. “Cell Walls of the Phycobionts Trebouxia and Pseudotrebouxia: Constituents and Their Localization.” The Lichenologist 16 (02): 129–144. doi:10.1017/S002428298400030X.
  • Kranner, I., Beckett, R. Hochman A., and T. H. Nash III. 2008. “Desiccation-Tolerance in Lichens: A Review.” The Bryologist 111 (4): 576–593. doi:10.1639/0007-2745-111.4.576.
  • Kulkarni, S. K., ed. 2015. Nanotechnology: Principles and Practices. Switzerland: Springer.
  • Law, K. L. 2017. “Plastics in the Marine Environment.” Annual Review of Marine Science 9 (1): 205–229. doi:10.1146/annurev-marine-010816-060409.
  • León, V., J. M. González-Domínguez, J. L. Fierro, M. Prato, and E. Vázquez. 2016. “Production and Stability of Mechanochemically Exfoliated Graphene in Water and Culture Media.” Nanoscale 8 (30): 14548–14555. doi:10.1039/C6NR03246J.
  • Liu, Y., A. Beyer, and R. Aebersold. 2016. “On the Dependency of Cellular Protein Levels on mRNA Abundance.” Cell 165 (3): 535–550. doi:10.1016/j.cell.2016.03.014.
  • Lüttge, U., and B. Büdel. 2010. “Resurrection Kinetics of Photosynthesis in Desiccation-Tolerant Terrestrial Green Algae (Chlorophyta) on Tree Bark.” Plant Biology 12 (3): 437–444. doi:10.1111/j.1438-8677.2009.00249.x.
  • Maier, T., P. Güell, and L. Serrano. 2009. “Correlation of mRNA and Protein in Complex Biological Samples.” FEBS Letters 583 (24): 3966–3973. doi:10.1016/j.febslet.2009.10.036.
  • Martín-de-Lucía, I., M. C. Campos-Mañas, A. Agüera, F. Leganes, F. Fernandez-Piñas, and R. Rosal. 2018. “Combined Toxicity of Graphene Oxide and Wastewater to the Green Alga Chlamydomonas reinhardtii.” Environmental Science: Nano 5: 1729–1744. doi:10.1039/C8EN00138C.
  • Mogera, U., R. Dhanya, R. Pujar, C. Narayana, and G. U. Kulkarni. 2015. “Highly Decoupled Graphene Multilayers: Turbostraticity at Its Best.” The Journal of Physical Chemistry Letters 6 (21): 4437–4443. doi:10.1021/acs.jpclett.5b02145.
  • Montagner, A., S. Bosi, E. Tenori, M. Bidussi, A. A. Alshatwi, M. Tretiach, M. Prato, and Z. Syrgiannis. 2016. “Ecotoxicological Effects of Graphene–based Materials.” 2D Materials 4 (1): 012001. doi:10.1088/2053-1583/4/1/012001.
  • Montagner, A. 2017. Ecotoxicological effects of Graphene-Based Materials. Thesis (PhD). University of Trieste.
  • Muggia, L., F. Candotto Carniel, and M. Grube. 2016. The Lichen Photobiont Trebouxia: Towards and Appreciation of Species Diversity and Molecular Studies. In: M. Grube, J. Seckbach and L. Muggia, eds. Algal and cyanobacteria symbioses. London, UK: World Scientific, 111–146.
  • Murchie, E. H., and T. Lawson. 2013. “Chlorophyll Fluorescence Analysis: a Guide to Good Practice and Understanding Some New Applications.” Journal of Experimental Botany 64 (13): 3983–3998. doi:10.1093/jxb/ert208.
  • Nogueira, P. F. M., D. Nakabayashi, and V. Zucolotto. 2015. “The Effects of Graphene Oxide on Green Algae Raphidocelis subcapitata.” Aquatic Toxicology 166: 29–35. doi:10.1016/j.aquatox.2015.07.001.
  • Northcote, D. H., K. J. Goulding, and R. W. Horne. 1958. “The Chemical Composition and Structure of the Cell Wall of Chlorella pyrenoidosa.” Biochemical Journal 70 (3): 391–397. doi:10.1042/bj0700391.
  • Novoselov, K. S., V. I. Fal′Ko, L. Colombo, P. R. Gellert, M. G. Schwab, and K. Kim. 2012. “A Roadmap for Graphene.” Nature 490 (7419): 192–200. doi:10.1038/nature11458.[10.1038/nature11458]
  • OECD. 2011. Test No. 201: Freshwater Alga and Cyanobacteria, Growth Inhibition Test. Paris: Organisation for Economic Co-operation and Development. doi:10.1787/9789264069923-en.
  • Oukarroum, A., W. Zaidi, M. Samadani, and D. Dewez. 2017. “Toxicity of Nickel Oxide Nanoparticles on a Freshwater Green Algal Strain of Chlorella vulgaris.” BioMed Research International 2017: 1. doi:10.1155/2017/9528180.
  • Ouyang, S., X. Hu, and Q. Zhou. 2015. “Envelopment-internalization Synergistic Effects and Metabolic Mechanism of Graphene Oxide on Single-cell Chlorella vulgaris Are Dependent on the Nanomaterial Particle Size.” ACS Applied Materials & Interfaces 7 (32): 18104–18112. doi:10.1021/acsami.5b05328.
  • Pan, S., H. Tian, S. R. S. Dangal, Z. Ouyang, B. Tao, W. Ren, C. Lu, and S. Running. 2014. “Modeling and Monitoring Terrestrial Primary Production in a Changing Global Environment: Toward a Multiscale Synthesis of Observation and Simulation.” Advances in Meteorology 2014: 1. doi:10.1155/2014/965936.
  • Pelin, M., L. Fusco, C. Martín, S. Sosa, J. Frontiñán-Rubio, J. M. González-Domínguez, M. Durán-Prado, E. Vázquez, M. Prato, and A. Tubaro. 2018. “Graphene and Graphene Oxide Induce ROS Production in Human HaCaT Skin Keratinocytes: the Role of Xanthine Oxidase and NADH Dehydrogenase.” Nanoscale 10 (25): 11820–11830. doi:10.1039/c8nr02933d.
  • Potts, M. 1994. “Desiccation Tolerance of Prokaryotes.” Microbiology and Molecular Biology Reviews 58 (4): 755–805. doi:10.1093/icb/45.5.800.
  • Pretti, C., M. Oliva, R. D. Pietro, G. Monni, G. Cevasco, F. Chiellini, C. Pomelli, and C. Chiappe. 2014. “Ecotoxicity of Pristine Graphene to Marine Organisms.” Ecotoxicology and Environmental Safety 101: 138–145. doi:10.1016/j.ecoenv.2013.11.008.
  • Rana, R. M., A. Iqbal, F. M. Wattoo, M. A. Khan, and H. Zhang. 2018. HSP70 Mediated Stress Modulation in Plants. In: A. A. A.Asea and P. Kaureds eds. Heat Shock Proteins and Stress. Cham, CH; Springer, 281–290.
  • R Development Core Team 2015., R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing,.
  • Roy, R.,. A. Parashar, M. Bhuvaneshwari, N. Chandrasekaran, and A. Mukherjee. 2016. “Differential Effects of P25 TiO2 Nanoparticles on Freshwater Green Microalgae: Chlorella and Scenedesmus Species.” Aquatic Toxicology 176: 161–171. doi:10.1016/j.aquatox.2016.04.021.
  • Samek, O., A. Jonáš, Z. Pilát, P. Zemánek, L. Nedbal, J. Tříska, P. Kotas, and M. Trtílek. 2010. “Raman Microspectroscopy of Individual Algal Cells: sensing Unsaturation of Storage Lipids in Vivo.” Sensors 10 (9): 8635–8651. doi:10.3390/s100908635.
  • Suppi, S.,. K. Kasemets, A. Ivask, K. Künnis-Beres, M. Sihtmäe, I. Kurvet, V. Aruoja, and A. Kahru. 2015. “A Novel Method for Comparison of Biocidal Properties of Nanomaterials to Bacteria, Yeasts and Algae.” Journal of Hazardous Materials 286: 75–84. doi:10.1016/j.jhazmat.2014.12.027.
  • Tang, Y., J. Tian, S. Li, C. Xue, C. Xue, D. Yin, and S. Yu. 2015. “Combined Effects of Graphene Oxide and Cd on the Photosynthetic Capacity and Survival of Mycrocystis aeruginosa.” Science of the Total Environment 532: 154–161. doi:10.1016/j.scitotenv.2015.05.081.
  • Tarhanen, S., T. Holopainen, and J. Oksanen. 1997. “Ultrastructural Changes and Electrolyte Leakage from Ozone Fumigated Epiphytic Lichens.” Annals of Botany 80 (5): 611–621. doi:10.1006/anbo.1997.0480.
  • Torrisi, F., Hasan, T., Wu, W. Sun, Z. Lombardo, A. Kulmala T. S. Hsieh G., S. Jung, et al. 2012. “Inkjet-Printed Graphene Electronics.” ACS Nano 6 (4): 2992–3006. doi:10.1021/nn2044609.
  • Tretiach, M., P. Adamo, R. Bargagli, L. Baruffo, L. Carletti, P. Crisafulli, S. Giordano, P. Modenesi, S. Orlando, and E. Pittao. 2007. “Lichen and Moss Bags as Monitoring Devices in Urban Areas. Part I: Influence of Exposure on Sample Vitality.” Environmental Pollution 146 (2): 380–391. doi:10.1016/j.envpol.2006.03.046.
  • Wang, S. B., Q. Hu, M. Sommerfeld, and F. Chen. 2004. “Cell Wall Proteomics of the Green Alga Haematococcus pluvialis (Chlorophyceae).” Proteomics 4 (3): 692–708. doi:10.1002/pmic.200300634.
  • Wellburn, A. R. 1994. “The Spectral Determination of Chlorophylls a and b as Well as Total Carotenoids Using Various Solvents with Spectrophotometers of Different Resolution.” Journal of Plant Physiology 144 (3): 307–313. doi:10.1016/S0176-1617(11)81192-2.
  • Wick, P., A. E. Louw-Gaume, M. Kucki, H. F. Krug, K. Kostarelos, B. Fadeel, K. A. Dawson., et al. 2014. “Classification Framework for Graphene-Based Materials.” Angewandte Chemie International Edition 53 (30): 7714–7718. doi:10.1002/anie.201403335.
  • Wu, H. C., D. L. Luo, F. Vignols, and T. L. Jinn. 2012. “Heat Shock- induced Biphasic Ca2+ Signature and OsCaM1-1 Nuclear Localization Mediate Downstream Signalling in Acquisition of Thermotolerance in Rice (Oryza sativa L.).” Plant Cell & Environment 35 (9): 1543–1557. doi:10.1111/j.1365-3040.2012.02508.x.
  • Yamamoto, M., M. Fujishita, A. Hirata, and S. Kawano. 2004. “Regeneration and Maturation of Daughter Cell Walls in the Autospore-forming Green Alga Chlorella vulgaris (Chlorophyta, Trebouxiophyceae).” Journal of Plant Research 117 (4): 257–264. doi:10.1007/s10265-004-0154-6.
  • Yamamoto, Y.,. Y. Kinoshita, and I. Yoshimura. 2002. Photobiont culturing. In: I. Kranner, R.P. Beckett, and A. K. Varma, eds. Protocols in Lichenology. Culturing, Biochemistry, Ecophysiology and Use in Biomonitoring. Heidelberg, D: Springer, 34–42.
  • Yan, L., Z. Gu, and Y. Zhao. 2013. “Chemical Mechanisms of the Toxicological Properties of Nanomaterials: generation of Intracellular Reactive Oxygen Species.” Chemistry - An Asian Journal 8 (10): 2342–2353. doi:10.1002/asia.201300542.
  • Yancey, P., M. Clark, S. Hand, R. Bowlus, and G. Somero. 1982. “Living with Water Stress: evolution of Osmolyte Systems.” Science 217 (4566): 1214–1222. doi:10.1126/science.7112124.
  • Yu, H. Y., T. Ziegelhoffer, J. Osipiuk, S. J. Ciesielski, M. Baranowski, M. Zhou, A. Joachimiak, and E. A. Craig. 2015. “Roles of Intramolecular and Intermolecular Interactions in Functional Regulation of the HSP70 J-Protein Co-chaperone Sis1.” Journal of Molecular Biology 427 (7): 1632–1643. doi:10.1016/j.jmb.2015.02.007.
  • Zeng, H., L. Xu, A. Singh, H. Wang, L. Du, and B. W. Poovaiah. 2015. “Involvement of Calmodulin and Calmodulin-like Proteins in Plant Responses to Abiotic Stresses.” Frontiers in Plant Science 6: 600. doi:10.3389/fpls.2015.00600].
  • Zhang, Y., T. Meng, X. Guo, R. Yang, X. Si, and J. Zhou. 2018. “Humic Acid Alleviates the Ecotoxicity of Graphene-family Materials on the Freshwater Microalgae Scenedesmus obliquus.” Chemosphere 197: 749–758. doi:10.1016/j.chemosphere.2018.01.051.