N⁶-methyladenosine-dependent primary microRNA-126 processing activated PI3K-AKT-mTOR pathway drove the development of pulmonary fibrosis induced by nanoscale carbon black particles in rats

References

• Alarcon, C. R., H. Goodarzi, H. Lee, X. Liu, S. Tavazoie, and S. F. Tavazoie. 2015a. “HNRNPA2B1 Is a Mediator of m(6)A-Dependent Nuclear RNA Processing Events.” Cell 162(6): 1299–1308. doi:10.1016/j.cell.2015.08.011.
   PubMed Web of Science ®Google Scholar
• Alarcón, Claudio R., Hyeseung Lee, Hani Goodarzi, Nils Halberg, and Sohail F. Tavazoie. 2015b. “Hani Goodarzi, Nils Halberg, and Sohail F. Tavazoie. 2015. 'N6-Methyladenosine Marks Primary microRNAs for Processing.” Nature 519(7544): 482–485. doi:10.1038/nature14281.
   PubMed Web of Science ®Google Scholar
• Ambros, V. 2004. “The Functions of Animal microRNAs.” Nature 431(7006): 350–355. doi:10.1038/nature02871.
   PubMed Web of Science ®Google Scholar
• Andre, E., T. Stoeger, S. Takenaka, M. Bahnweg, B. Ritter, E. Karg, B. Lentner, C. Reinhard, H. Schulz, and M. Wjst. 2006. “Inhalation of Ultrafine Carbon Particles Triggers Biphasic Pro-Inflammatory Response in the Mouse Lung.” European Respiratory Journal 28(2): 275–285. doi:10.1183/09031936.06.00071205.
   PubMed Web of Science ®Google Scholar
• Anjilvel, S., and B. Asgharian. 1995. “A Multiple-Path Model of Particle Deposition in the Rat Lung.” Fundamental and Applied Toxicology 28: 41–50. doi:10.1006/faat.1995.1144.
   PubMedGoogle Scholar
• Badulak, J. H., M. Schurr, A. Sauaia, A. Ivashchenko, and E. Peltz. 2018. “Defining the Criteria for Intubation of the Patient with Thermal Burns.” Burns: Journal of the International Society for Burn Injuries 44(3): 531–538. doi:10.1016/j.burns.2018.02.016.
   PubMed Web of Science ®Google Scholar
• Bartel, D. P. 2009. “MicroRNAs: Target Recognition and Regulatory Functions.” Cell 136(2): 215–233. doi:10.1016/j.cell.2009.01.002.
   PubMed Web of Science ®Google Scholar
• Bates, J. H. T. 2017. “CORP: Measurement of Lung Function in Small Animals.” Journal of Applied Physiology (Bethesda, Md.: 1985) 123(5): 1039–1046. doi:10.1152/japplphysiol.00243.2017.
   PubMed Web of Science ®Google Scholar
• Blanco, L. N., and L. Frank. 1994. “Development of Gas-Exchange Surface Area in Rat Lung. The Effect of Alveolar Shape.” American Journal of Respiratory and Critical Care Medicine 149(3 Pt 1): 759–766. doi:10.1164/ajrccm.149.3.8118647.
   PubMed Web of Science ®Google Scholar
• Blanco, L. N., D. Massaro, and G. D. Massaro. 1991. “Alveolar Size, Number, and Surface Area: Developmentally Dependent Response to 13% O2.” American Journal of Physiology-Lung Cellular and Molecular Physiology 261: L370–L377. doi:10.1152/ajplung.1991.261.6.L370.
   Google Scholar
• Bourdon, J. A., A. Williams, B. Kuo, I. Moffat, P. A. White, S. Halappanavar, U. Vogel, H. Wallin, and C. L. Yauk. 2013. “Gene Expression Profiling to Identify Potentially Relevant Disease Outcomes and Support Human Health Risk Assessment for Carbon Black Nanoparticle Exposure.” Toxicology 303: 83–93. doi:10.1016/j.tox.2012.10.014.
   PubMed Web of Science ®Google Scholar
• Brennecke, J., D. R. Hipfner, A. Stark, R. B. Russell, and S. M. Cohen. 2003. “Bantam Encodes a Developmentally Regulated microRNA That Controls Cell Proliferation and Regulates the Proapoptotic Gene Hid in Drosophila.” Cell 113(1): 25–36. doi:10.1016/S0092-8674(03)00231-9.
   PubMed Web of Science ®Google Scholar
• Brown, E. J., P. A. Beal, C. T. Keith, J. Chen, T. B. Shin, and S. L. Schreiber. 1995. “Control of p70 s6 Kinase by Kinase Activity of FRAP In Vivo.” Nature 377(6548): 441–446. doi:10.1038/377441a0.
   PubMed Web of Science ®Google Scholar
• Cantley, L. C. 2002. “The Phosphoinositide 3-Kinase Pathway.” Science (New York, N.Y.) 296(5573): 1655–1657. doi:10.1126/science.296.5573.1655.
   PubMed Web of Science ®Google Scholar
• Cesta, M. F., J. P. Ryman-Rasmussen, D. G. Wallace, T. Masinde, G. Hurlburt, A. J. Taylor, and J. C. Bonner. 2010. “Bacterial Lipopolysaccharide Enhances PDGF Signaling and Pulmonary Fibrosis in Rats Exposed to Carbon Nanotubes.” American Journal of Respiratory Cell and Molecular Biology 43(2): 142–151. doi:10.1165/rcmb.2009-0113OC.
   PubMed Web of Science ®Google Scholar
• Challa, A. A., and B. Stefanovic. 2011. “A Novel Role of Vimentin Filaments: Binding and Stabilization of Collagen mRNAs.” Molecular and Cellular Biology 31: 3773. doi:10.1128/MCB.05263-11.
   PubMed Web of Science ®Google Scholar
• Chen, Shangya, Guanqun Cui, Cheng Peng, Martin F. Lavin, Xiaoying Sun, Enguo Zhang, Ye Yang, Yingjun Guan, Zhongjun Du, and Hua Shao. 2018. “Transplantation of Adipose-Derived Mesenchymal Stem Cells Attenuates Pulmonary Fibrosis of Silicosis via anti-Inflammatory and anti-Apoptosis Effects in Rats.” Stem Cell Research & Therapy 9(1): 110. doi:10.1186/s13287-018-0846-9.
   PubMedGoogle Scholar
• Chen, Tong, Ya-Juan Hao, Ying Zhang, Miao-Miao Li, Meng Wang, Weifang Han, Yongsheng Wu, Ying Lv, Jie Hao, Libin Wang., et al. 2015. “m(6)A RNA methylation is regulated by microRNAs and promotes reprogramming to pluripotency.” Cell Stem Cell 16(3): 289–301. doi:10.1016/j.stem.2015.01.016.
   PubMed Web of Science ®Google Scholar
• Chu, C., Zhou, L. H. Xie, Z. Pei, M. Zhang, M. Wu, S. Zhang, L. Wang, C. Zhao, L. Shi, N., et al. 2019. “Pulmonary Toxicities from a 90-Day Chronic Inhalation Study with Carbon Black Nanoparticles in Rats Related to the Systemical Immune Effects.” International Journal of Nanomedicine 14: 2995–3013. doi:10.2147/IJN.S198376.
   PubMed Web of Science ®Google Scholar
• Denli, A. M., B. B. Tops, R. H. Plasterk, R. F. Ketting, and G. J. Hannon. 2004. “Processing of Primary microRNAs by the Microprocessor Complex.” Nature 432(7014): 231–235. doi:10.1038/nature03049.
   PubMed Web of Science ®Google Scholar
• Dennis, P. B., S. Fumagalli, and G. Thomas. 1999. “Target of Rapamycin (TOR): Balancing the Opposing Forces of Protein Synthesis and Degradation.” Current Opinion in Genetics & Development 9(1): 49–54.
   PubMed Web of Science ®Google Scholar
• Du, Hao, Ya Zhao, Jinqiu He, Yao Zhang, Hairui Xi, Mofang Liu, Jinbiao Ma, and Ligang Wu. 2016. “YTHDF2 Destabilizes m6A-Containing RNA through Direct Recruitment of the CCR4–NOT Deadenylase Complex.” Nature Communications 7(1): 12626. doi:10.1038/ncomms12626.
   PubMedGoogle Scholar
• Elder, Alison, Robert Gelein, Jacob N. Finkelstein, Kevin E. Driscoll, Jack Harkema, and Günter OberdöRster, 2005. “Effects of Subchronically Inhaled Carbon Black in Three Species. I. Retention Kinetics, Lung Inflammation, and Histopathology.” Toxicological Sciences 88(2): 614–629. doi:10.1093/toxsci/kfi327.
   PubMed Web of Science ®Google Scholar
• Fu, Y., D. Dominissini, G. Rechavi, and C. He. 2014. “Gene Expression Regulation Mediated through Reversible m(6)a RNA Methylation.” Nature Reviews Genetics 15(5): 293–306. doi:10.1038/nrg3724.
   PubMed Web of Science ®Google Scholar
• Fustin, Jean-Michel, Masao Doi, Yoshiaki Yamaguchi, Hayashi Hida, Shinichi Nishimura, Minoru Yoshida, Takayuki Isagawa, Masaki Suimye Morioka, Hideaki Kakeya, Ichiro Manabe., et al. 2013. “RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock.” Cell 155(4): 793–806. doi:10.1016/j.cell.2013.10.026.
   PubMed Web of Science ®Google Scholar
• Ganguly, K., Ettehadieh, D. S. Upadhyay, S. Takenaka, T. Adler, E. Karg, F. Krombach, W. G. Kreyling, H. Schulz, O. Schmid, T., et al. 2017. “Early Pulmonary Response is Critical for Extra-Pulmonary Carbon Nanoparticle Mediated Effects: Comparison of Inhalation versus Intra-Arterial Infusion Exposures in Mice.” Particle and Fibre Toxicology 14(1): 19. doi:10.1186/s12989-017-0200-x.
   PubMedGoogle Scholar
• Gebert, Luca F. R., and Ian J. MacRae. 2018. “Regulation of microRNA Function in Animals.” Nature Reviews Molecular Cell Biology 20(1): 21–37. doi:10.1038/s41580-018-0045-7.
   Web of Science ®Google Scholar
• Geissler, E. K., and H. J. Schlitt. 2010. “The Potential Benefits of Rapamycin on Renal Function, Tolerance, Fibrosis, and Malignancy following Transplantation.” Kidney International 78(11): 1075–1079. doi:10.1038/ki.2010.324.
   PubMed Web of Science ®Google Scholar
• Geula, Shay, Sharon Moshitch-Moshkovitz, Dan Dominissini, Abed AlFatah Mansour, Nitzan Kol, Mali Salmon-Divon, Vera Hershkovitz, Eyal Peer, Nofar Mor, Yair S. Manor., et al. 2015. “Stem Cells. m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency toward Differentiation.” Science 347(6225): 1002–1006. doi:10.1126/science.1261417.
   PubMed Web of Science ®Google Scholar
• Gregory, Richard I., Kai-ping Yan, Govindasamy Amuthan, Thimmaiah Chendrimada, Behzad Doratotaj, Neil Cooch, and Ramin Shiekhattar. 2004. “The Microprocessor Complex Mediates the Genesis of microRNAs.” Nature 432(7014): 235–240. doi:10.1038/nature03120.
   PubMed Web of Science ®Google Scholar
• Gui, X., L. Sun, L. Gu, H. Cai, and H. Chen. 2018. “Leptin Promotes Pulmonary Fibrosis Development by Inhibiting Autophagy via PI3K/Akt/mTOR Pathway.” Biochemical and Biophysical Research Communications 498(3): 660–666. doi:10.1016/j.bbrc.2018.03.039.
   PubMed Web of Science ®Google Scholar
• Guo, C., J. F. Sah, L. Beard, J. K. Willson, S. D. Markowitz, and K. Guda. 2010. “The Noncoding RNA, miR-126, Suppresses the Growth of Neoplastic Cells by Targeting Phosphatidylinositol 3-Kinase Signaling and Is Frequently Lost in Colon Cancers.” Genes, Chromosomes and Cancer 47: 939–946. doi:10.1002/gcc.20596.
   Web of Science ®Google Scholar
• Han, J., Y. Lee, K. H. Yeom, Y. K. Kim, H. Jin, and V. N. Kim. 2004. “The Drosha-DGCR8 Complex in Primary microRNA Processing.” Genes & Development 18(24): 3016–3027. doi:10.1101/gad.1262504.
   PubMed Web of Science ®Google Scholar
• Han, J., Y. Lee, K. H. Yeom, J. W. Nam, I. Heo, J. K. Rhee, S. Y. Sohn, Y. Cho, B. T. Zhang, and V. N. Kim. 2006. “Molecular Basis for the Recognition of Primary microRNAs by the Drosha-DGCR8 Complex.” Cell 125(5): 887–901. doi:10.1016/j.cell.2006.03.043.
   PubMed Web of Science ®Google Scholar
• Hardie, W. D., S. W. Glasser, and J. S. Hagood. 2009. “Emerging Concepts in the Pathogenesis of Lung Fibrosis.” The American Journal of Pathology 175(1): 3–16. doi:10.2353/ajpath.2009.081170.
   PubMed Web of Science ®Google Scholar
• Hay, N., and N. Sonenberg. 2004. “Upstream and Downstream of mTOR.” Genes & Development 18(16): 1926–1945. doi:10.1101/gad.1212704.
   PubMed Web of Science ®Google Scholar
• Heinrich, U., R. Fuhst, S. Rittinghausen, O. Creutzenberg, B. Bellmann, W. Koch, and K. Levsen. 1995. “Chronic Inhalation Exposure of Wistar Rats and Two Different Strains of Mice to Diesel Engine Exhaust, Carbon Black, and Titanium Dioxide.” Inhalation Toxicology 7(4): 533–556. doi:10.3109/08958379509015211.
   Web of Science ®Google Scholar
• Hubbs, Ann F., Sargent, Linda M. Dale W. Porter, Tina M. Sager, Bean T. Chen, David G. Frazer, Vincent Castranova, Krishnan Sriram, Timothy R. Nurkiewicz, Steven H. Reynolds, Lori A. Battelli, et al. 2013. “Nanotechnology: Toxicologic Pathology.” Toxicologic Pathology 41(2): 395–409. doi:10.1177/0192623312467403.
   PubMed Web of Science ®Google Scholar
• Hussain, S., S. Sangtian, S. M. Anderson, R. J. Snyder, J. D. Marshburn, A. B. Rice, J. C. Bonner, and S. Garantziotis. 2014. “Inflammasome Activation in Airway Epithelial Cells after Multi-Walled Carbon Nanotube Exposure Mediates a Profibrotic Response in Lung Fibroblasts.” Particle and Fibre Toxicology 11(1): 28. doi:10.1186/1743-8977-11-28.
   PubMedGoogle Scholar
• Ji, Xiaoming, Baiqun Wu, Jingjing Fan, Ruhui Han, Chen Luo, Ting Wang, Jingjin Yang, Lei Han, Baoli Zhu, Dong Wei., et al. 2015. “The anti-Fibrotic Effects and Mechanisms of MicroRNA-486-5p in Pulmonary Fibrosis.” Scientific Reports 5: 14131. doi:10.1038/srep14131.
   PubMed Web of Science ®Google Scholar
• Kamata, H., Tasaka, S. K. Inoue, K. Miyamoto, Y. Nakano, H. Shinoda, Y. Kimizuka, H. Fujiwara, M. Ishii, N. Hasegawa, R., et al. 2011. “Carbon Black Nanoparticles Enhance Bleomycin-Induced Lung Inflammatory and Fibrotic Changes in Mice.” Experimental Biology and Medicine 236(3): 315–324. doi:10.1258/ebm.2011.010180.
   Web of Science ®Google Scholar
• Kang, H. R., C. G. Lee, R. J. Homer, and J. A. Elias. 2007. “Semaphorin 7A Plays a Critical Role in TGF-beta1-induced pulmonary fibrosis.” The Journal of Experimental Medicine 204(5): 1083–1093. doi:10.1084/jem.20061273.
   PubMed Web of Science ®Google Scholar
• Kim, Kevin K., Matthias C. Kugler, Paul J. Wolters, Liliane Robillard, Michael G. Galvez, Alexis N. Brumwell, Dean Sheppard, and Harold A. Chapman. 2006. “Alveolar Epithelial Cell Mesenchymal Transition Develops in Vivo during Pulmonary Fibrosis and Is Regulated by the Extracellular Matrix.” Proceedings of the National Academy of Sciences of the United States of America 103(35): 13180. doi:10.1073/pnas.0605669103.
   PubMed Web of Science ®Google Scholar
• Kodali, V. K., Roberts, J. R. M. Shoeb, M. G. Wolfarth, L. Bishop, T. Eye, M. Barger, K. A. Roach, S. Friend, D. Schwegler-Berry, B. T., et al. 2017. “Acute in Vitro and in Vivo Toxicity of a Commercial Grade Boron Nitride Nanotube Mixture.” Nanotoxicology 11(8): 1040–1058. doi:10.1080/17435390.2017.1390177.
   PubMed Web of Science ®Google Scholar
• Koike, E., H. Takano, K. I. Inoue, R. Yanagisawa, M. Sakurai, H. Aoyagi, R. Shinohara, and T. Kobayashi. 2008. “Pulmonary Exposure to Carbon Black Nanoparticles Increases the Number of Antigen-Presenting Cells in Murine Lung.” International Journal of Immunopathology and Pharmacology 21(1): 35–42. doi:10.1177/039463200802100105.
   PubMed Web of Science ®Google Scholar
• Kwon, S. C., T. A. Nguyen, Y. G. Choi, M. H. Jo, S. Hohng, V. N. Kim, and J. S. Woo. 2016. “Structure of Human DROSHA.” Cell 164(1-2): 81–90. doi:10.1016/j.cell.2015.12.019.
   PubMed Web of Science ®Google Scholar
• Lai, Y. L., and H. Chou. 2000. “Respiratory Mechanics and Maximal Expiratory Flow in the Anesthetized Mouse.” Journal of Applied Physiology 88(3): 939–943. doi:10.1152/jappl.2000.88.3.939.
   PubMed Web of Science ®Google Scholar
• Lam, Anna P., Annette S. Flozak, Susan Russell, Jun Wei, Manu Jain, Gökhan M. Mutlu, G R Scott Budinger, Carol A. Feghali-Bostwick, John Varga, and Cara J. Gottardi. 2011. “Nuclear β-Catenin Is Increased in Systemic Sclerosis Pulmonary Fibrosis and Promotes Lung Fibroblast Migration and Proliferation.” American Journal of Respiratory Cell and Molecular Biology 45(5): 915. doi:10.1165/rcmb.2010-0113OC.
   PubMed Web of Science ®Google Scholar
• Laplante, M., and D. M. Sabatini. 2012. “mTOR Signaling in Growth Control and Disease.” Cell 149(2): 274–293. doi:10.1016/j.cell.2012.03.017.
   PubMed Web of Science ®Google Scholar
• Li, L., and A. H. Ross. 2007. “Why Is PTEN an Important Tumor Suppressor?” Journal of Cellular Biochemistry 102(6): 1368–1374. doi:10.1002/jcb.21593.
   PubMed Web of Science ®Google Scholar
• Lin, S., J. Choe, P. Du, R. Triboulet, and R. I. Gregory. 2016. “The m(6)a Methyltransferase METTL3 Promotes Translation in Human Cancer Cells.” Molecular Cell 62(3): 335–345. doi:10.1016/j.molcel.2016.03.021.
   PubMed Web of Science ®Google Scholar
• Lin, W., Huang, W. T. Zhu, M. Hu, B. Brunekreef, Y. Zhang, X. Liu, H. Cheng, U. Gehring, C. Li, X., et al. 2011. “Acute Respiratory Inflammation in Children and Black Carbon in Ambient Air before and during the 2008 Beijing Olympics.” Environmental Health Perspectives 119(10): 1507–1512. doi:10.1289/ehp.1103461.
   PubMed Web of Science ®Google Scholar
• Liu, Gang, Arnaud Friggeri, Yanping Yang, Jadranka Milosevic, Qiang Ding, Victor J. Thannickal, Naftali Kaminski, and Edward Abraham. 2010. “miR-21 Mediates Fibrogenic Activation of Pulmonary Fibroblasts and Lung Fibrosis.” The Journal of Experimental Medicine 207(8): 1589–1597. doi:10.1084/jem.20100035.
   PubMed Web of Science ®Google Scholar
• Liu, Jianzhao, Yanan Yue, Dali Han, Xiao Wang, Ye Fu, Liang Zhang, Guifang Jia, Miao Yu, Zhike Lu, Xin Deng., et al. 2014. “A METTL3-METTL14 Complex Mediates Mammalian Nuclear RNA N6-Adenosine Methylation.” Nature Chemical Biology 10(2): 93–95. doi:10.1038/nchembio.1432.
   PubMed Web of Science ®Google Scholar
• Luai, Huleihel, Ahmi, Ben Yehudah Milosevic Jadranka, Yu Guoying, Pandit Kusum, Sakamoto Koji, Yousef Hanadie, LeJeune Megan, Tiffany, A. Coon., et al. 2014. “Let-7d microRNA Affects Mesenchymal Phenotypic Properties of Lung Fibroblasts.” American Journal of Physiology Lung Cellular & Molecular Physiology 306: 534–542. doi:10.1152/ajplung.00149.2013.
   Web of Science ®Google Scholar
• Ma, J. Z., F. Yang, C. C. Zhou, F. Liu, J. H. Yuan, F. Wang, T. T. Wang, Q. G. Xu, W. P. Zhou, and S. H. Sun. 2017. “METTL14 Suppresses the Metastatic Potential of Hepatocellular Carcinoma by Modulating N(6) -Methyladenosine-Dependent Primary MicroRNA Processing.” Hepatology 65(2): 529–543.
   PubMed Web of Science ®Google Scholar
• Meyer, K. D., and S. R. Jaffrey. 2014. “The Dynamic Epitranscriptome: N6-Methyladenosine and Gene Expression Control.” Nature Reviews Molecular Cell Biology 15(5): 313–326. doi:10.1038/nrm3785.
   PubMed Web of Science ®Google Scholar
• Muller, J., F. Huaux, N. Moreau, P. Misson, J. F. Heilier, M. Delos, M. Arras, A. Fonseca, J. B. Nagy, and D. Lison. 2005. “Respiratory Toxicity of Multi-Wall Carbon Nanotubes.” Toxicology and Applied Pharmacology 207(3): 221–231. doi:10.1016/j.taap.2005.01.008.
   PubMed Web of Science ®Google Scholar
• Nau, C. A., G. T. Taylor, and C. H. Lawrence. 1976. “Properties and Physiological Effects of Thermal Carbon Black.” Journal of Occupational and Environmental Medicine 18: 732. doi:10.1097/00043764-197611000-00008.
   Web of Science ®Google Scholar
• Nguyen, T. A., M. H. Jo, Y. G. Choi, J. Park, S. C. Kwon, S. Hohng, V. N. Kim, and J. S. Woo. 2015. “Functional Anatomy of the Human Microprocessor.” Cell 161(6): 1374–1387. doi:10.1016/j.cell.2015.05.010.
   PubMed Web of Science ®Google Scholar
• Nikula, K. J., M. B. Snipes, E. B. Barr, W. C. Griffith, R. F. Henderson, and J. L. Mauderly. 1995. “Comparative Pulmonary Toxicities and Carcinogenicities of Chronically Inhaled Diesel Exhaust and Carbon Black in F344 Rats.” Fundamental and Applied Toxicology 25: 80–94. doi:10.1006/faat.1995.1042.
   PubMedGoogle Scholar
• Oglesby, Irene K., Isabella M. Bray, Sanjay H. Chotirmall, Raymond L. Stallings, Shane J. O’Neill, Noel G. McElvaney, and Catherine M. Greene. 2010. “miR-126 Is Downregulated in Cystic Fibrosis Airway Epithelial Cells and Regulates TOM1 Expression.” The Journal of Immunology 184(4): 1702–1709. doi:10.4049/jimmunol.0902669.
   PubMed Web of Science ®Google Scholar
• Patsenker, E., V. Schneider, M. Ledermann, H. Saegesser, C. Dorn, C. Hellerbrand, and F. Stickel. 2011. “Potent Antifibrotic Activity of mTOR Inhibitors Sirolimus and Everolimus but Not of Cyclosporine a and Tacrolimus in Experimental Liver Fibrosis.” Journal of Hepatology 55(2): 388–398. doi:10.1016/j.jhep.2010.10.044.
   PubMed Web of Science ®Google Scholar
• Saputra, D., J. H. Yoon, H. Park, Y. Heo, H. Yang, E. J. Lee, S. Lee, C. W. Song, and K. Lee. 2014. “Inhalation of Carbon Black Nanoparticles Aggravates Pulmonary Inflammation in Mice.” Toxicology Research 30(2): 83–90.
   PubMedGoogle Scholar
• Scotton, Chris J., and Rachel C. Chambers. 2007. “Molecular Targets in Pulmonary Fibrosis.” Chest 132(4): 1311–1321. doi:10.1378/chest.06-2568.
   PubMed Web of Science ®Google Scholar
• Shalaby, K. H., L. G. Gold, T. F. Schuessler, J. G. Martin, and A. Robichaud. 2010. “Combined Forced Oscillation and Forced Expiration Measurements in Mice for the Assessment of Airway Hyperresponsiveness.” Respiratory Research 11(1): 82. doi:10.1186/1465-9921-11-82.
   PubMedGoogle Scholar
• Song, Lei, Dan Li, Yue Gu, Zhong-Mei Wen, Jing Jie, Dan Zhao, and Li-Ping Peng. 2016. “MicroRNA-126 Targeting PIK3R2 Inhibits NSCLC A549 Cell Proliferation, Migration, and Invasion by Regulation of PTEN/PI3K/AKT Pathway.” Clinical Lung Cancer 17(5): e65–e75. doi:10.1016/j.cllc.2016.03.012.
   PubMed Web of Science ®Google Scholar
• Stoeger, T., C. Reinhard, S. Takenaka, A. Schroeppel, E. Karg, B. Ritter, J. Heyder, and H. Schulz. 2006. “Instillation of Six Different Ultrafine Carbon Particles Indicates a Surface Area Threshold Dose for Acute Lung Inflammation in Mice.” Environmental Health Perspectives 114(3): 328–333. doi:10.1289/ehp.8266.
   PubMed Web of Science ®Google Scholar
• Stoeger, T., S. Takenaka, B. Frankenberger, B. Ritter, E. Karg, K. Maier, H. Schulz, and O. Schmid. 2009. “Deducing in Vivo Toxicity of Combustion-Derived Nanoparticles from a Cell-Free Oxidative Potency Assay and Metabolic Activation of Organic Compounds.” Environmental Health Perspectives 117(1): 54–60. doi:10.1289/ehp.11370.
   PubMed Web of Science ®Google Scholar
• Stone, K. C., R. R. Mercer, B. A. Freeman, L. Y. Chang, and J. D. Crapo. 1992. “Distribution of Lung Cell Numbers and Volumes between Alveolar and Nonalveolar Tissue.” American Review of Respiratory Disease 146(2): 454–456. doi:10.1164/ajrccm/146.2.454.
   PubMedGoogle Scholar
• Takayama, T.,. K. Fujita, K. Suzuki, M. Sakaguchi, M. Fujie, E. Nagai, S. Watanabe, A. Ichiyama, and Y. Ogawa. 2003. “Control of Oxalate Formation from L-Hydroxyproline in Liver Mitochondria.” Journal of the American Society of Nephrology 14(4): 939–946. doi:10.1097/01.ASN.0000059310.67812.4F.
   PubMed Web of Science ®Google Scholar
• Teeguarden, Justin G., Bobbie-Jo Webb-Robertson, Katrina M. Waters, Ashley R. Murray, Elena R. Kisin, Susan M. Varnum, Jon M. Jacobs, Joel G. Pounds, Richard C. Zanger, and Anna A. Shvedova. 2011. “Comparative Proteomics and Pulmonary Toxicity of Instilled Single-Walled Carbon Nanotubes, Crocidolite Asbestos, and Ultrafine Carbon Black in Mice.” Toxicological Sciences 120(1): 123. doi:10.1093/toxsci/kfq363.
   PubMed Web of Science ®Google Scholar
• Verma, Dave K., Alec C. Ritchie, and David C.F. Muir. 2008. “Dust Content of Lungs and Its Relationships to Pathology, Radiology and Occupational Exposure in Ontario Hardrock Miners.” American Journal of Industrial Medicine 51(7): 524–531. doi:10.1002/ajim.20589.
   PubMed Web of Science ®Google Scholar
• Visvanathan, Abhirami, and Kumaravel Somasundaram. 2018. “mRNA Traffic Control Reviewed: N6-Methyladenosine (m6A) Takes the Driver's Seat.” BioEssays 40(1): 1700093. doi:10.1002/bies.201700093.
   Web of Science ®Google Scholar
• Wang, Xiao-Chun, Li-Qing Du, Li-Li Tian, Hai-Liang Wu, Xiao-Yan Jiang, Heng Zhang, De-Guan Li, Yue-Ying Wang, Hong-Ying Wu, Qing-fen Yi She., et al. 2011. “Expression and Function of miRNA in Postoperative Radiotherapy Sensitive and Resistant Patients of Non-Small Cell Lung Cancer.” Lung Cancer (Amsterdam, Netherlands) 72(1): 92–99. doi:10.1016/j.lungcan.2010.07.014.
   PubMed Web of Science ®Google Scholar
• Wang, Yang, Yue Li, Julia I. Toth, Matthew D. Petroski, Zhaolei Zhang, and Jing Crystal Zhao. 2014. “N6-Methyladenosine Modification Destabilizes Developmental Regulators in Embryonic Stem Cells.” Nature Cell Biology 16(2): 191–198. doi:10.1038/ncb2902.
   PubMed Web of Science ®Google Scholar
• Wei, L., Q. Chen, A. Guo, J. Fan, R. Wang, and H. Zhang. 2018. “Asiatic Acid Attenuates CCl4-Induced Liver Fibrosis in Rats by Regulating the PI3K/AKT/mTOR and Bcl-2/Bax Signaling Pathways.” International Immunopharmacology 60: 1. doi:10.1016/j.intimp.2018.04.016.
   PubMed Web of Science ®Google Scholar
• Wu, Qiuyun, Lei Han, Weiwen Yan, Xiaoming Ji, Ruhui Han, Jingjin Yang, Jiali Yuan, and Chunhui Ni. 2016. “miR-489 Inhibits Silica-Induced Pulmonary Fibrosis by Targeting MyD88 and Smad3 and Is Negatively Regulated by lncRNA CHRF.” Scientific Reports 6: 30921. doi:10.1038/srep30921.
   PubMed Web of Science ®Google Scholar
• Xia, Hong, Deanna Diebold, Richard Nho, David Perlman, Jill Kleidon, Judy Kahm, Svetlana Avdulov, Mark Peterson, John Nerva, Peter Bitterman., et al. 2008. “Pathological Integrin Signaling Enhances Proliferation of Primary Lung Fibroblasts from Patients with Idiopathic Pulmonary Fibrosis.” The Journal of Experimental Medicine 205(7): 1659–1672. doi:10.1084/jem.20080001.
   PubMed Web of Science ®Google Scholar
• Yu, S. Y., L. Liu, P. Li, and J. Li. 2013. “Rapamycin Inhibits the mTOR/p70S6K Pathway and Attenuates Cardiac Fibrosis in Adriamycin-Induced Dilated Cardiomyopathy.” The Thoracic and Cardiovascular Surgeon 61: 223–228. doi:10.1055/s-0032-1311548.
   PubMed Web of Science ®Google Scholar
• Zhang, R., Dai, Y. X. Zhang, Y. Niu, T. Meng, Y. Li, H. Duan, P. Bin, M. Ye, X. Jia, M., et al. 2014. “Reduced Pulmonary Function and Increased Pro-Inflammatory Cytokines in Nanoscale Carbon Black-Exposed Workers.” Particle and Fibre Toxicology 11(1): 73. doi:10.1186/s12989-014-0073-1.
   PubMedGoogle Scholar
• Zhao, Boxuan Simen, Ian A. Roundtree, and Chuan He. 2017. “Post-transcriptional gene regulation by mRNA modifications.” Nature Reviews. Molecular Cell Biology 18(1): 31. doi:10.1038/nrm.2016.132.
   PubMed Web of Science ®Google Scholar
• Zhao, Xu, Ying Yang, Bao-Fa Sun, Yue Shi, Xin Yang, Wen Xiao, Ya-Juan Hao, Xiao-Li Ping, Yu-Sheng Chen, Wen-Jia Wang., et al. 2014. “ FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis.” Cell Research 24(12): 1403–1419. doi:10.1038/cr.2014.151.
   PubMed Web of Science ®Google Scholar