456
Views
17
CrossRef citations to date
0
Altmetric
Articles

Occupational exposure to graphene and silica nanoparticles. Part II: pilot study to identify a panel of sensitive biomarkers of genotoxic, oxidative and inflammatory effects on suitable biological matrices

, , , , , , , , , , ORCID Icon, ORCID Icon & show all
Pages 223-237 | Received 18 Jun 2020, Accepted 08 Nov 2020, Published online: 29 Dec 2020

References

  • American Thoracic Society, European Respiratory Society. 2005. “ATS/ERS Recommendations for Standardized Procedures for the Online and Offline Measurement of Exhaled Lower Respiratory Nitric Oxide and Nasal Nitric Oxide.” American Journal of Respiratory and Critical Care Medicine 171: 912–930.
  • Andreoli, R., P. Manini, M. Corradi, A. Mutti, and W. M. A. Niessen. 2003. “Determination of Patterns of Biologically Relevant Aldehydes in Exhaled Breath Condensate of Healthy Subjects by Liquid Chromatography/Atmospheric Chemical Ionization Tandem Mass Spectrometry.” Rapid Communications in Mass Spectrometry: RCM 17 (7): 637–645. doi:10.1002/rcm.960.
  • Andreoli, R., A. Mutti, M. Goldoni, P. Manini, P. Apostoli, and G. De Palma. 2011. “Reference Ranges of Urinary Biomarkers of Oxidized Guanine in (2'-Deoxy)Ribonucleotides and Nucleic Acids.” Free Radical Biology and Medicine 50 (2): 254–261. doi:10.1016/j.freeradbiomed.2010.11.009.
  • Andreoli, R., G. Spatari, D. Pigini, D. Poli, I. Banda, M. Goldoni, M. G. Riccelli, et al. 2015. “Urinary Biomarkers of Exposure and of Oxidative Damage in Children Exposed to Low Airborne Concentrations of Benzene.” Environmental Research 142: 264–272. doi:10.1016/j.envres.2015.07.003.
  • Beard, J. D., A. Erdely, M. M. Dahm, M. A. de Perio, M. E. Birch, D. E. Evans, J. E. Fernback, et al. 2018. “Carbon Nanotube and Nanofiber Exposure and Sputum and Blood Biomarkers of Early Effect among U.S. workers.” Environment International 116: 214–228. doi:10.1016/j.envint.2018.04.004.
  • Bhattacharya, K., G. Kiliç, P. M. Costa, and B. Fadeel. 2017. “Cytotoxicity Screening and Cytokine Profiling of Nineteen Nanomaterials Enables Hazard Ranking and Grouping Based on Inflammogenic Potential.” Nanotoxicology 11 (6): 809–826. doi:10.1080/17435390.2017.1363309.
  • Boccuni, F., R. Ferrante, F. Tombolini, C. Natale, A. Gordiani, S. Sabella, and S. Iavicoli. 2020. “Occupational Exposure to Graphene and Silica Nanoparticles: Part I – Workplace Measurements and Samplings.” Nanotoxicology doi:10.1080/17435390.2020.1834634.
  • Bodini, A., D. Peroni, L. Vicentini, A. Loiacono, E. Baraldi, L. Ghiro, M. Corradi, R. Alinovi, A. L. Boner, and G. L. Piacentini. 2004. “Exhaled Breath Condensate Eicosanoids and Sputum Eosinophils in Asthmatic Children: A Pilot Study.” Pediatric Allergy and Immunology: Official Publication of the European Society of Pediatric Allergy and Immunology 15(1): 26–31. doi:10.1046/j.0905-6157.2003.00097.x.
  • Bonassi, S., E. Coskun, M. Ceppi, C. Lando, C. Bolognesi, S. Burgaz, N. Holland, et al. 2011. “The Human Micronucleus Project on ExfoLiated Buccal Cells (HUMN(XL)): The Role of Life-Style, Host Factors, Occupational Exposures, Health Status, and Assay Protocol.” Mutation Research/Reviews in Mutation Research 728 (3): 88–97. doi:10.1016/j.mrrev.2011.06.005.
  • Burgum, M. J., M. J. D. Clift, S. J. Evans, N. Hondow, M. Miller, S. B. Lopez, A. Williams, A. Tarat, G. J. Jenkins, and S. H. Doak. 2020. “In Vitro Primary-Indirect Genotoxicity in Bronchial Epithelial Cells Promoted by Industrially Relevant Few-Layer Graphene.” Small: e2002551. doi:10.1002/smll.202002551.
  • Carubbi, C., E. Masselli, E. Calabrò, E. Bonati, C. Galeone, R. Andreoli, M. Goldoni, et al. 2019. “Sulphurous Thermal Water Inhalation Impacts Respiratory Metabolic Parameters in Heavy Smokers.” International Journal of Biometeorology 63 (9): 1209–1216. doi:10.1007/s00484-019-01737-7.
  • Cavallo, D., A. Ciervo, A. M. Fresegna, R. Maiello, P. Tassone, G. Buresti, S. Casciardi, S. Iavicoli, and C. L. Ursini. 2015. “Investigation on Cobalt-Oxide Nanoparticles Cyto-Genotoxicity and Inflammatory Response in Two Types of Respiratory Cells.” Journal of Applied Toxicology: JAT 35 (10): 1102–1113. doi:10.1002/jat.3133.
  • Cavallo, D., G. Tranfo, C. L. Ursini, A. M. Fresegna, A. Ciervo, R. Maiello, E. Paci, et al. 2018. “Biomarkers of Early Genotoxicity and Oxidative Stress for Occupational Risk Assessment of Exposure to Styrene in the Fibreglass Reinforced Plastic Industry.” Toxicology Letters 298: 53–59. doi:10.1016/j.toxlet.2018.06.006.
  • Cavallo, D.,C. L. Ursini, A. Setini, C. Chianese, A. Cristaudo, and S. Iavicoli. 2005. “DNA Damage and TNFalpha Cytokine Production in Hairdressers with Contact Dermatitis.” Contact Dermatitis 53 (3): 125–129. doi:10.1111/j.0105-1873.2005.00672.
  • Collins, A. R. 2014. “Measuring Oxidative Damage to DNA with Comet Assay.” Biochimica et Biophysica Acta (BBA) – General Subjects 1840 (2): 794–800. doi:10.1016/j.bbagen.2013.04.022.
  • Collins, A. R., S. J. Duthie, and V. L. Dobson. 1993. “Direct Enzymic Detection of Endogenous Oxidative Base Damage in Human Lymphocyte DNA.” Carcinogenesis 14 (9): 1733–1735. doi:10.1093/carcin/14.9.1733.
  • Corradi, M., P. Gergelova, and A. Mutti. 2010. “Use of Exhaled Breath Condensate to Investigate Occupational Lung Diseases.” Current Opinion in Allergy and Clinical Immunology 10 (2): 93–98. doi:10.1097/ACI.0b013e3283357fb7.
  • Corradi, M., D. Poli, I. Banda, S. Bonini, P. Mozzoni, S. Pinelli, R. Alinovi, et al. 2015. “Exhaled Breath Analysis in Suspected Cases of Non-Small-Cell Lung Cancer: A Cross-Sectional Study.” Journal of Breath Research 9 (2): 027101. doi:10.1088/1752-7155/9/2/027101.
  • Del Rio Castillo, A. E.,V. Pellegrinia, A. Ansaldo, F. Ricciardella, H. Suna, L. Marasco, J. Buha, et al. 2016. High-yield production of 2D crystals by wet-jet milling. Patent application No. PCT/IB2016/057108.
  • Demir, E., and R. Marcos. 2018. “Toxic and Genotoxic Effects of Graphene and multi-walled carbon nanotubes.” Journal of Toxicology and Environmental Health A 81 (14): 645–660. doi:10.1080/15287394.2018.1477314.
  • Du, Z., D. Zhao, L. Jing, G. Cui, M. Jin, Y. Li, X. Liu, et al. 2013. “Cardiovascular Toxicity of Different Sizes Amorphous Silica Nanoparticles in Rats after Intratracheal Instillation.” Cardiovascular Toxicology 13 (3): 194–207. doi:10.1007/s12012-013-9198-y.
  • Duan, J., Y. Yu, Y. Li, Y. Yu, Y. Li, X. Zhou, P. Huang, and Z. Sun. 2013. “Toxic Effect of Silica Nanoparticles on Endothelial Cells through DNA Damage Response via Chk1-Dependent G2/M Checkpoint.” PLoS One 8 (4): e62087. doi:10.1371/journal.pone.0062087.
  • Ema, M., M. Gamo, and K. Honda. 2017. “A Review of Toxicity Studies on graphene-based nanomaterials in laboratory animals.” Regulatory Toxicology and Pharmacology 85: 7–24. doi:10.1016/j.yrtph.2017.01.011.
  • Fatkhutdinova, L. M., T. O. Khaliullin, O. L. Vasil'yeva, R. R. Zalyalov, I. G. Mustafin, E. R. Kisin, M. E. Birch, N. Yanamala, and A. A. Shvedova. 2016. “Fibrosis Biomarkers in Workers Exposed to MWCNTs.” Toxicology and Applied Pharmacology 299: 125–131. doi:10.1016/j.taap.2016.02.016.
  • Gonzales, L., and M. Kirsh-Volders. 2016. “Biomonitoring of Genotroxic Effects for Human Exposure to Nanomaterials: The Challenge Ahead.” Mutation Research 768: 14–26.
  • Guo, C., J. Wang, M. Yang, Y. Li, S. Cui, X. Zhou, Y. Li, and Z. Sun. 2017. “Amorphous Silica Nanoparticles Induce Malignant Transformation and Tumorigenesis of Human Lung Epithelial Cells via P53 Signaling.” Nanotoxicology 11 (9–10): 1176–1194. doi:10.1080/17435390.2017.1403658.
  • Horváth, I., J. Hunt, P. J. Barnes, K. Alving, A. Antczak, E. Baraldi, G. Becher, ATS/ERS Task Force on Exhaled Breath Condensate, et al. 2005. “Task Force on Exhaled Breath Condensate. 2005. “Exhaled Breath Condensate: methodological Recommendations and Unresolved Questions.” The European Respiratory Journal 26 (3): 523–548. doi:10.1183/09031936.05.00029705.
  • Iavicoli, I., L. Fontana, V. Leso, M. C. Macrini, and D. Pelclova. 2020. ” “Fractional Exhaled Nitric Oxide and Nanomaterial Exposure in Workplaces.” Current Medicinal Chemistry 27. doi:10.2174/0929867327666200320154545.
  • Låg, M., T. Skuland, A. Godymchuk, T. H. T. Nguyen, H. L. T. Pham, and M. Refsnes. 2018. “Silica Nanoparticle-Induced Cytokine Responses in BEAS-2B and HBEC3-KT Cells: Significance of Particle Size and Signalling Pathways in Different Lung Cell Cultures.” Basic &Clinical Pharmacology &Toxicology 122 (6): 620–632. doi:10.1111/bcpt.12963.
  • Liao, H.-Y., Y.-T. Chung, C.-H. Lai, S.-L. Wang, H.-C. Chiang, L.-A. Li, T.-C. Tsou, et al. 2014. “Six-Month Follow-up Study of Health Markers of Nanomaterials among Workers Handling Engineered Nanomaterials.” Nanotoxicology 8 (sup1): 100–110. 10.3109/17435390.2013.858793.
  • Lin, H., D.-K. Ding-Kun Ji, M. A. Lucherelli, G. Reina, S. Ippolito, P. Samorì, and A. Bianco. 2020. “Comparative Effects of Graphene and Molybdenum Disulfide on Human Macrophage Toxicity.” Small (Weinheim an Der Bergstrasse, Germany) 16 (35): e2002194. doi:10.1002/smll.202002194.
  • Liou, S. H.,Y. C. Chen, H. Y. Liao, C. J. Wang, J. S. Chen, and H. L. Lee. 2016. “Increased Levels of Oxidative Stress Biomarkers in Metal Oxides Nanomaterial-Handling Workers.” Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals 21 (7): 600–606. 10.3109/135475 0X.2016.1160432.
  • Liou, S.-H., T.-C. Tsou, S.-L. Wang, L.-A. Li, H.-C. Chiang, W.-F. Li, P.-P. Lin, et al. 2012. “Epidemiological Study of Health Hazards among Workers Handling Engineered Nanomaterials.” Journal of Nanoparticle Research 14 (8): 878. doi:10.1007/s11051-012-0878-5.
  • Liou, S. H.,W. T. Wu, H. Y. Liao, C. Y. Chen, C. Y. Tsai, W. T. Jung, and H. L. Lee. 2017. “Global DNA Methylation and Oxidative Stress Biomarkers in Workers Exposed to Metal Oxide Nanoparticles.” Journal of Hazardous Materials 331: 329–335. 10.1016/j.jhazmat.2017.02.042.
  • Malvindi, M. A., V. Brunetti, G. Vecchio, A. Galeone, R. Cingolani, and P. P. Pompa. 2012. “SiO2 Nanoparticles Biocompatibility and Their Potential for Gene Delivery and Silencing.” Nanoscale 4 (2): 486–495. 10.1039/c1nr11269d.
  • Malvindi, M. A., V. De Matteis, A. Galeone, V. Brunetti, G. C. Anyfantis, A. Athanassiou, R. Cingolani, et al. 2014. “Toxicity Assessment of Silica Coated Iron Oxide Nanoparticles and Biocompatibility Improvement by Surface Engineering.” PLoS One 9 (1): e85835. doi:10.1371/journal.pone.0085835.
  • Murugadoss, S., D. Lison, L. Godderis, S. Van Den Brule, J. Mast, F. Brassinne, N. Sebaihi, and P. H. Hoet. 2017. “Toxicology of Silica Nanoparticles: An update.” Archives of Toxicology 91 (9): 2967–3010.
  • Ou, L.,B. Song, H. Liang, J. Liu, X. Feng, B. Deng, T. Sun, and L. Shao. 2016. “Toxicity of Graphene-Family Nanoparticles: A General Review of the Origins and Mechanisms.” Particle and Fibre Toxicology 13 (1): 57. doi:19.1186/s12989-016-0168-y.
  • Pelclova, D., V. Zdimal, Z. Fenclova, S. Vlckova, F. Turci, I. Corazzari, P. Kacer, et al. 2016. “Markers of Oxidative Damage of Nucleic Acids and Proteins among Workers Exposed to TiO2 (Nano) Particles.” Occupational and Environmental Medicine 73 (2): 110–118. doi:10.1136/oemed-2015-103161.
  • Pelclova, D., V. Zdimal, P. Kacer, Z. Fenclova, S. Vlckova, K. Syslova, T. Navratil, et al. 2016. “Oxidative Stress Markers Are Elevated in Exhaled Breath Condensate of Workers Exposed to Nanoparticles during Iron Oxide Pigment Production.” Journal of Breath Research 10 (1): 016004. doi:10.1088/1752-7155/10/1/016004,016004.
  • Pelclova, D., V. Zdimal, P. Kacer, N. Zikova, M. Komarc, Z. Fenclova, S. Vlckova, et al. 2017. “Markers of Lipid Oxidative Damage in the Exhaled Breath Condensate of Nano TiO2Production Workers.” Nanotoxicology 11 (1): 52–63. doi:10.1080/17435390.2016.1262921.
  • Pelin, M., S. Sosa, M. Prato, and A. Tubaro. 2018. “Occupational Exposure to Graphene Based Nanomaterials: Risk Assessment.” Nanoscale 10 (34): 15894–15903. doi:10.1039/c8nr04950e.
  • Poulsen, H. E., L. L. Nadal, K. Broedbaek, P. E. Nielsen, and A. Weimann. 2014. “Detection and Interpretation of 8-oxodG and 8-oxoGua in Urine, Plasma and Cerebrospinal Fluid.” Biochimica et Biophysica Acta 1840 (2): 801–808. doi:10.1016/j.bbagen.2013.06.009.
  • Refsnes, M., T. Skuland, E. Lilleaas, J. Øvrevik, and M. Låg. 2019. “Concentration-Dependent Cytokine Responses of Silica Nanoparticles and Role of ROS in Human Lung Epithelial Cells.” Basic & Clinical Pharmacology & Toxicology 125 (3): 304–314. doi:10.1111/bcpt.13221.
  • Roberts, J. R., R. R. Mercer, A. B. Stefaniak, M. S. Seehra, U. K. Geddam, I. S. Chaudhuri, A. Kyrlidis, et al. 2016. “Evaluation of Pulmonary and Systemic Toxicity following Lung Exposure to Graphite Nanoplates: A Member of the Graphene-Based Nanomaterial Family.” Particle and Fibre Toxicology13 (1): 34. doi:10.1186/s12989-016-0145-5.
  • Rossnerova, A., K. Honkova, D. Pelclova, V. Zdimal, J. A. Hubacek, I. Chvojkova, K. Vrbova, et al. 2020. “DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles.” International Journal of Molecular Sciences 21 (7): 2420. doi:10.3390/ijms21072420.
  • Rossnerova, A., D. Pelclova, V. Zdimal, P. Rossner, F. Elzeinova, K. Vrbova, J. Topinka, et al. 2019. “The Repeated Cytogenetic Analysis of Subjects Occupationally Exposed to Nanoparticles: A Pilot Study.” Mutagenesis 34 (3): 253–263. doi:10.1093/mutage/gez016.
  • Schulte, P. A., V. Leso, M. Niang, and I. Iavicoli. 2019. “Current State of Knowledge on the Health Effects of Engineered Nanomaterials in Workers: A Systematic Review of Human Studies and Epidemiological Investigations.” Scandinavian Journal of Work, Environment & Health 45 (3): 217–238. doi:10.5271/sjweh.3800.
  • Song, Y., X. Li, and X. Du. 2009. “Exposure to Nanoparticles is Related to Pleural Effusion, Pulmonary Fibrosis and Granuloma.” The European Respiratory Journal 34(3): 559–567. doi:10.1183/09031936.00178308.
  • Song, Y., X. Li, L. Wang, Y. Rojanasakul, V. Castranova, H. Li, J. Ma, et al. 2011. “Nanomaterials in Humans: identification, Characteristics, and Potential Damage.” Toxicologic Pathology 39 (5): 841–849. 10.1177/0192623311413787.
  • Tabish, T. A., S. Shaowei Zhang, and P. G. Winyard. 2018. “Developing the Next Generation of Graphene-Based Platforms for Cancer Therapeutics: The Potential Role of Reactive Oxygen Species.” Redox Biology 15: 34–40. doi:10.1016/j.redox.2017.11.018.
  • Ursini, C. L., D. Cavallo, A. M. Fresegna, A. Ciervo, R. Maiello, G. Buresti, S. Casciardi, S. Bellucci, and S. Iavicoli. 2014a. “Differences in Cytotoxic, Genotoxic, and Inflammatory Response of Bronchial and Alveolar Human Lung Epithelial Cells to Pristine and COOH-Functionalized Multiwalled Carbon Nanotubes.” BioMed Research International 2014: 359506. doi:10.1155/2014/359506.
  • Ursini, C. L., D. Cavallo, A. M. Fresegna, A. Ciervo, R. Maiello, P. Tassone, G. Buresti, S. Casciardi, and S. Iavicoli. 2014b. “Evaluation of Cytotoxic, Genotoxic and Inflammatory Response in Human Alveolar and Bronchial Epithelial Cells Exposed to Titanium Dioxide nanoparticles.” Journal of Applied Toxicology 34 (11): 1209–1219. doi:10.1002/jat.3038.
  • Ursini, C. L., Omodeo Salè, E., A. M. Fresegna, A. Ciervo, C. Jemos, R. Maiello, G Buresti, et al. 2019. “Antineoplastic Drug Occupational Exposure: A New Integrated Approach to Evaluate Exposure and Early Genotoxic and Cytotoxic Effects by No-Invasive Buccal Micronucleus Cytome Assay Biomarker.” Toxicology Letters 316: 20–26. doi:10.1016/j.toxlet.2019.08.022.
  • World Health Organization. 2017. WHO Guidelines on Protecting Workers from Potential Risks of Manufactured Nanomaterials. Geneva, World Health Organization.
  • Wu, W. T., H. Y. Liao, Y. T. Chung, W. F. Li, T. C. Tsou, L. A. Li, M. H. Lin, J. J. Ho, T. N. Wu, and S. H. Liou. 2014. “Effect of Nanoparticles Exposure on Fractional Exhaled Nitric Oxide (FENO) in Workers Exposed to Nanomaterials.” International Journal of Molecular Sciences. 15 (1): 878–894. doi:10.3390/ijms15010878.
  • Wu, W. T., L. A. Li, T. C. Tsou, S. L. Wang, H. L. Lee, T. S. Shih, and S. H. Liou. 2019. “Longitudinal Follow-up of Health Effects among Workers Handling Engineered Nanomaterials: A Panel Study.” Environmental Health18 (1): 107. doi:10.1186/s12940-019-0542-y.
  • Yazdimamaghani, M., P. J. Moos, M. A. Dobrovolskaia, and H. Ghandehari. 2019. “Genotoxicity of Amorphous Silica Nanoparticles: Status and Prospects.” Nanomedicine: Nanotechnology, Biology, and Medicine 16: 106–125. doi:10.1016/j.nano.2018.11.013.
  • Zhang, R.,Y. Dai, X. Zhang, Y. Niu, T. Meng, Y. Li, H. Duan, et al. 2014. “Reduced Pulmonary Function and Increased Pro-Inflammatory Cytokines in Nanoscale Carbon Black-Exposed Workers.” Particle and Fibre Toxicology. 11: 73 doi:10.1186/s12989-014-0073-1.
  • Zhang, B., P. Wei, Z. Zhou, and T. Wei. 2016. “Interactions of Graphene with Mammalian Cells: Molecular Mechanisms and Biomedical insights.” Advanced Drug Delivery Review 105 (Pt B): 145–162. doi:10.1016/j.addr.2016.08.009.
  • Zhao, L., Y. Zhu, Z. Chen, H. Xu, J. Zhou, S. Tang, Z. Xu, et al. 2018. “Cardiopulmonary Effects Induced by Occupational Exposure to Titanium Dioxide Nanoparticles.” Nanotoxicology 12 (2): 169–184. doi:10.1080/17435390.2018.1425502.
  • Zijno, A., D. Cavallo, G. Di Felice, J. Ponti, B. Barletta, C. Butteroni, S. Corinti, et al. 2020. “Use of a Common European Approach for Nanomaterials' Testing to Support Regulation: A Case Study on Titanium and Silicon Dioxide Representative Nanomaterials.” Journal of Applied Toxicology: JAT 40 (11): 1511–1525. doi:10.1002/jat.4002.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.