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Articles

Transgenic zebrafish larvae as a non-rodent alternative model to assess pro-inflammatory (neutrophil) responses to nanomaterials

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Pages 333-354 | Received 06 Apr 2022, Accepted 07 Jun 2022, Published online: 07 Jul 2022

References

  • Abbasalipourkabir, R., H. Moradi, S. Zarei, S. Asadi, A. Salehzadeh, A. Ghafourikhosroshahi, M. Mortazavi, and N. Ziamajidi. 2015. “Toxicity of Zinc Oxide Nanoparticles on Adult Male Wistar Rats.” Food and Chemical Toxicology 84: 154–160. doi:10.1016/j.fct.2015.08.019.
  • Adamcakova-Dodd, A., L. V. Stebounova, J. Kim, S. U. Vorrink, A. P. Ault, P. T. O’Shaughnessy, V. H. Grassian, and P. S. Thorne. 2014. “Toxicity Assessment of Zinc Oxide Nanoparticles Using Sub-Acute and Sub-Chronic Murine Inhalation Models.” Particle and Fibre Toxicology 11: 15. doi:10.1186/1743-8977-11-15.
  • Akter, M., M. T. Sikder, M. M. Rahman, A. K. M. A. Ullah, K. F. B. Hossain, S. Banik, T. Hosokawa, T. Saito, and M. Kurasaki. 2018. “A Systematic Review on Silver Nanoparticles-Induced Cytotoxicity: Physicochemical Properties and Perspectives.” Journal of Advanced Research 9: 1–16. doi:10.1016/j.jare.2017.10.008.
  • Almansour, M. I., M. A. Alferah, Z. A. Shraideh, and B. M. Jarrar. 2017. “Zinc Oxide Nanoparticles Hepatotoxicity: Histological and Histochemical Study.” Environmental Toxicology and Pharmacology 51: 124–130. doi:10.1016/j.etap.2017.02.015.
  • Asharani, P. V., Y. Lian Wu, Z. Gong, and S. Valiyaveettil. 2008a. “Toxicity of Silver Nanoparticles in Zebrafish Models.” Nanotechnology 19 (25): 255102. doi:10.1088/0957-4484/19/25/255102.
  • Asharani, P. V., N. G. B. Serina, M. H. Nurmawati, Y. L. Wu, Z. Gong, and S. Valiyaveettil. 2008b. “Impact of Multi-Walled Carbon Nanotubes on Aquatic Species.” Journal of Nanoscience and Nanotechnology 8 (7): 3603–3609. doi:10.1166/jnn.2008.432.
  • Awasthi, K. K., A. Awasthi, R. Verma, I. Soni, K. Awasthi, and P. J. John. 2015. “Silver Nanoparticles and Carbon Nanotubes Induced DNA Damage in Mice Evaluated by Single Cell Gel Electrophoresis.” Macromolecular Symposia 357 (1): 210–217. doi:10.1002/masy.201500018.
  • Bai, C., and M. Tang. 2020. “Toxicological Study of Metal and Metal Oxide Nanoparticles in Zebrafish.” Journal of Applied Toxicology 40 (1): 37–63. doi:10.1002/jat.3910.
  • Bai, W., Z. Zhang, W. Tian, X. He, Y. Ma, Y. Zhao, and Z. Chai. 2010. “Toxicity of Zinc Oxide Nanoparticles to Zebrafish Embryo: A Physicochemical Study of Toxicity Mechanism.” Journal of Nanoparticle Research 12 (5): 1645–1654. doi:10.1007/s11051-009-9740-9.
  • Bar-Ilan, O., R. M. Albrecht, V. E. Fako, and D. Y. Furgeson. 2009. “Toxicity Assessments of Multisized Gold and Silver Nanoparticles in Zebrafish Embryos.” Small 5 (16): 1897–1910. doi:10.1002/smll.200801716.
  • Bar-Ilan, O., C. C. Chuang, D. J. Schwahn, S. Yang, S. Joshi, J. A. Pedersen, R. J. Hamers, R. E. Peterson, and W. Heideman. 2013. “TiO2 Nanoparticle Exposure and Illumination during Zebrafish Development: Mortality at Parts per Billion Concentrations.” Environmental Science & Technology 47 (9): 4726–4733. doi:10.1021/es304514r.
  • Bar-Ilan, O., K. M. Louis, S. P. Yang, J. A. Pedersen, R. J. Hamers, R. E. Peterson, and W. Heideman. 2012. “Titanium Dioxide Nanoparticles Produce Phototoxicity in the Developing Zebrafish.” Nanotoxicology 6 (6): 670–679. doi:10.3109/17435390.2011.604438.
  • Barros-Becker, F., P.-Y. Lam, R. Fisher, and A. Huttenlocher. 2017. “Live Imaging Reveals Distinct Modes of Neutrophil and Macrophage Migration within Interstitial Tissues.” Journal of Cell Science 130 (22): 3801–3808. doi:10.1242/jcs.206128.
  • Bates, J. M., J. Akerlund, E. Mittge, and K. Guillemin. 2007. “Intestinal Alkaline Phosphatase Detoxifies Lipopolysaccharide and Prevents Inflammation in Zebrafish in Response to the Gut Microbiota.” Cell Host & Microbe 2 (6): 371–382. doi:10.1016/j.chom.2007.10.010.
  • Benard, E. L., A. M van der. Sar, F. Ellett, G. J. Lieschke, H. P. Spaink, and A. H. Meijer. 2012. “Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens.” Journal of Visualized Experiments: JoVE (61): 3781. doi:10.3791/3781.
  • Bernut, A., M. Nguyen-Chi, I. Halloum, J.-L. Herrmann, G. Lutfalla, and L. Kremer. 2016. “Mycobacterium abscessus-Induced Granuloma Formation is Strictly Dependent on TNF Signaling and Neutrophil Trafficking.” PLOS Pathogens 12 (11): e1005986. doi:10.1371/journal.ppat.1005986.
  • Bilberg, K., M. B. Hovgaard, F. Besenbacher, and E. Baatrup. 2012. “In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio) [WWW Document].” Journal of Toxicology 2012: 293784. doi:10.1155/2012/293784.
  • Bondarenko, O., K. Juganson, A. Ivask, K. Kasemets, M. Mortimer, and A. Kahru. 2013. “Toxicity of Ag, CuO and ZnO Nanoparticles to Selected Environmentally Relevant Test Organisms and Mammalian Cells In Vitro: A Critical Review.” Archives of Toxicology 87 (7): 1181–1200. doi:10.1007/s00204-013-1079-4.
  • Bos, R. van den, S. Cromwijk, K. Tschigg, J. Althuizen, J. Zethof, R. Whelan, G. Flik, and M. Schaaf. 2019. Early life glucocorticoid exposure modulates immune function in zebrafish (Danio rerio) larvae. bioRxiv 2019.12.11.872903. doi:10.1101/2019.12.11.872903.
  • Brun, N. R., B. E. V. Koch, M. Varela, W. J. G. M. Peijnenburg, H. P. Spaink, and M. G. Vijver. 2018. “Nanoparticles Induce Dermal and Intestinal Innate Immune System Responses in Zebrafish Embryos.” Environmental Science: Nano 5 (4): 904–916. doi:10.1039/C8EN00002F.
  • Brun, N. R., M. Lenz, B. Wehrli, and K. Fent. 2014. “Comparative Effects of Zinc Oxide Nanoparticles and Dissolved Zinc on Zebrafish Embryos and Eleuthero-Embryos: Importance of Zinc Ions.” The Science of the Total Environment 476–477: 657–666. doi:10.1016/j.scitotenv.2014.01.053.
  • Buchan, K. D., M. van Gent, T. K. Prajsnar, N. V. Ogryzko, N. W. M. de Jong, J. Kolata, S. J. Foster, J. A. G. van Strijp, and S. A. Renshaw. 2020. Humanisation of the zebrafish C5a receptor confers targeting by human-specific staphylococcal virulence factors (preprint). Immunology. doi:10.1101/2020.02.18.955021.
  • Budama-Kilinc, Y., R. Cakir-Koc, T. Zorlu, B. Ozdemir, Z. Karavelioglu, A. C. Egil, and S. Kecel-Gunduz. 2018. Assessment of Nano-toxicity and Safety Profiles of Silver Nanoparticles. Silver Nanoparticles – Fabrication, Characterization and Applications. doi:10.5772/intechopen.75645.
  • Burden, N., K. Aschberger, Q. Chaudhry, M. J. D. Clift, S. H. Doak, P. Fowler, H. Johnston, R. Landsiedel, J. Rowland, and V. Stone. 2017. “The 3Rs as a Framework to Support a 21st Century Approach for Nanosafety Assessment.” Nano Today 12: 10–13. doi:10.1016/j.nantod.2016.06.007.
  • Carlson, C., S. M. Hussain, A. M. Schrand, L. K. Braydich-Stolle, K. L. Hess, R. L. Jones, and J. J. Schlager. 2008. “Unique Cellular Interaction of Silver Nanoparticles: Size-Dependent Generation of Reactive Oxygen Species.” The Journal of Physical Chemistry. B 112 (43): 13608–13619. doi:10.1021/jp712087m.
  • Cassar, S., I. Adatto, J. L. Freeman, J. T. Gamse, I. Iturria, C. Lawrence, A. Muriana, R. T. Peterson, S. Van Cruchten, and L. I. Zon. 2020. “Use of Zebrafish in Drug Discovery Toxicology.” Chemical Research in Toxicology 33 (1): 95–118. doi:10.1021/acs.chemrestox.9b00335.
  • Cho, W.-S., R. Duffin, C. A. Poland, A. Duschl, G. J. Oostingh, W. MacNee, M. Bradley, I. L. Megson, and K. Donaldson. 2012. “Differential Pro-Inflammatory Effects of Metal Oxide Nanoparticles and Their Soluble Ions In Vitro and In Vivo; Zinc and Copper Nanoparticles, but Not Their Ions, Recruit Eosinophils to the Lungs.” Nanotoxicology 6 (1): 22–35. doi:10.3109/17435390.2011.552810.
  • Choe, C. P., S. Y. Choi, Y. Kee, M. J. Kim, S. H. Kim, Y. Lee, H. C. Park, and H. Ro. 2021. “Transgenic Fluorescent Zebrafish Lines That Have Revolutionized Biomedical Research.” Laboratory Animal Research 37 (1): 26. doi:10.1186/s42826-021-00103-2.
  • Choi, J. S., R.-O. Kim, S. Yoon, and W.-K. Kim. 2016. “Developmental Toxicity of Zinc Oxide Nanoparticles to Zebrafish (Danio rerio): A Transcriptomic Analysis.” PLOS One 11 (8): e0160763. doi:10.1371/journal.pone.0160763.
  • Clemente, Z., V. L. S. S. Castro, M. A. M. Moura, C. M. Jonsson, and L. F. Fraceto. 2014. “Toxicity Assessment of TiO2 Nanoparticles in Zebrafish Embryos under Different Exposure Conditions.” Aquatic Toxicology 147: 129–139. doi:10.1016/j.aquatox.2013.12.024.
  • Cordero-Maldonado, M. L., D. Siverio-Mota, L. Vicet-Muro, I. M. Wilches-Arizábala, C. V. Esguerra, P. A. M. de Witte, and A. D. Crawford. 2013. “Optimization and Pharmacological Validation of a Leukocyte Migration Assay in Zebrafish Larvae for the Rapid In Vivo Bioactivity Analysis of anti-Inflammatory Secondary Metabolites.” PLOS One 8 (10): e75404. doi:10.1371/journal.pone.0075404.
  • Cvejic, A., C. Hall, M. Bak-Maier, M. V. Flores, P. Crosier, M. J. Redd, and P. Martin. 2008. “Analysis of WASp Function during the Wound Inflammatory Response – Live-Imaging Studies in Zebrafish Larvae.” Journal of Cell Science 121 (Pt 19): 3196–3206. doi:10.1242/jcs.032235.
  • Da Silva, E., Y. Kembouche, U. Tegner, A. Baun, and K. A. Jensen. 2019. “Interaction of Biologically Relevant Proteins with ZnO Nanomaterials: A Confounding Factor for In Vitro Toxicity Endpoints.” Toxicology In Vitro 56: 41–51. doi:10.1016/j.tiv.2018.12.016.
  • d'Amora, M., T. Schmidt, S. Konstantinidou, V. Raffa, F. De Angelis, and F. Tantussi. 2022. “Effects of Metal Oxide Nanoparticles in Zebrafish.” Oxidative Medicine and Cellular Longevity 2022: 3313016. doi:10.1155/2022/3313016.
  • Davis, J. M., M. Huang, M. R. Botts, C. M. Hull, and A. Huttenlocher. 2016. “A Zebrafish Model of Cryptococcal Infection Reveals Roles for Macrophages, Endothelial Cells, and Neutrophils in the Establishment and Control of Sustained Fungemia.” Infection and Immunity 84 (10): 3047–3062. doi:10.1128/IAI.00506-16.
  • de Oliveira, S., C. C. Reyes-Aldasoro, S. Candel, S. A. Renshaw, V. Mulero, and Â. Calado. 2013. “Cxcl8 (IL-8) Mediates Neutrophil Recruitment and Behavior in the Zebrafish Inflammatory Response.” Journal of Immunology 190 (8): 4349–4359. doi:10.4049/jimmunol.1203266.
  • Deng, Q., E. A. Harvie, and A. Huttenlocher. 2012. “Distinct Signalling Mechanisms Mediate Neutrophil Attraction to Bacterial Infection and Tissue Injury: H2O2-Independent Neutrophil Attraction to Infection.” Cellular Microbiology 14 (4): 517–528. doi:10.1111/j.1462-5822.2011.01738.x.
  • Deng, Q., M. Sarris, D. A. Bennin, J. M. Green, P. Herbomel, and A. Huttenlocher. 2013. “Localized Bacterial Infection Induces Systemic Activation of Neutrophils through Cxcr2 Signaling in Zebrafish.” Journal of Leukocyte Biology 93 (5): 761–769. doi:10.1189/jlb.1012534.
  • Díaz-Pascual, F., J. Ortíz-Severín, M. A. Varas, M. L. Allende, and F. P. Chávez. 2017. “In Vivo Host-Pathogen Interaction as Revealed by Global Proteomic Profiling of Zebrafish Larvae.” Frontiers in Cellular and Infection Microbiology 7: 334. doi:10.3389/fcimb.2017.00334.
  • Dodd, A., P. M. Curtis, L. C. Williams, and D. R. Love. 2000. “Zebrafish: Bridging the Gap between Development and Disease.” Human Molecular Genetics 9 (16): 2443–2449. doi:10.1093/hmg/9.16.2443.
  • Donnellan, S., L. Tran, H. Johnston, J. McLuckie, K. Stevenson, and V. Stone. 2016. “A Rapid Screening Assay for Identifying Mycobacteria Targeted Nanoparticle Antibiotics.” Nanotoxicology 10 (6): 761–769. doi:10.3109/17435390.2015.1124468.
  • Duan, J., Y. Yu, Yang. Li, Yanbo. Li, H. Liu, L. Jing, M. Yang, J. Wang, C. Li, and Z. Sun. 2016. “Low-Dose Exposure of Silica Nanoparticles Induces Cardiac Dysfunction via Neutrophil-Mediated Inflammation and Cardiac Contraction in Zebrafish Embryos.” Nanotoxicology 10 (5): 575–585. doi:10.3109/17435390.2015.1102981.
  • Duan, J., Y. Yu, H. Shi, L. Tian, C. Guo, P. Huang, X. Zhou, S. Peng, and Z. Sun. 2013. “Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae.” PLOS One 8 (9): e74606. doi:10.1371/journal.pone.0074606.
  • El Yamani, N., A. R. Collins, E. Rundén-Pran, L. M. Fjellsbø, S. Shaposhnikov, S. Zienolddiny, and M. Dusinska. 2017. “In Vitro Genotoxicity Testing of Four Reference Metal Nanomaterials, Titanium Dioxide, Zinc Oxide, Cerium Oxide and Silver: Towards Reliable Hazard Assessment.” Mutagenesis 32 (1): 117–126. doi:10.1093/mutage/gew060.
  • Elks, P. M., F. J. van Eeden, G. Dixon, X. Wang, C. C. Reyes-Aldasoro, P. W. Ingham, M. K. B. Whyte, S. R. Walmsley, and S. A. Renshaw. 2011. “Activation of Hypoxia-Inducible Factor-1 (Hif-1) Delays Inflammation Resolution by Reducing Neutrophil Apoptosis and Reverse Migration in a Zebrafish Inflammation Model.” Blood 118 (3): 712–722. doi:10.1182/blood-2010-12-324186.
  • Ellett, F., P. M. Elks, A. L. Robertson, N. V. Ogryzko, and S. A. Renshaw. 2015. “Defining the Phenotype of Neutrophils following Reverse Migration in Zebrafish.” Journal of Leukocyte Biology 98 (6): 975–981. doi:10.1189/jlb.3MA0315-105R.
  • Ellett, F., and D. Irimia. 2017a. “Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae.” Journal of Visualized Experiments: JoVE (130): 56498. doi:10.3791/56498.
  • Ellett, F., and D. Irimia. 2017b. “Microstructured Surface Arrays for Injection of Zebrafish Larvae.” Zebrafish 14 (2): 140–145. doi:10.1089/zeb.2016.1402.
  • Ellett, F., L. Pase, J. W. Hayman, A. Andrianopoulos, and G. J. Lieschke. 2011. “mpeg1 Promoter Transgenes Direct Macrophage-Lineage Expression in Zebrafish.” Blood 117 (4): e49–e56. doi:10.1182/blood-2010-10-314120.
  • Ellett, F., V. Pazhakh, L. Pase, E. L. Benard, H. Weerasinghe, D. Azabdaftari, S. Alasmari, A. Andrianopoulos, and G. J. Lieschke. 2018. “Macrophages Protect Talaromyces marneffei Conidia from Myeloperoxidase-Dependent Neutrophil Fungicidal Activity during Infection Establishment In Vivo.” PLOS Pathogens 14 (6): e1007063. doi:10.1371/journal.ppat.1007063.
  • European Commission. 2010. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes OJ L.
  • European Commission. 2011. Commission recommendation of 18 October 2011 on the definition of nanomaterial (2011/696/EU). Official Journal of the European Union.
  • Feng, Y., C. Santoriello, M. Mione, A. Hurlstone, and P. Martin. 2010. “Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation.” PLOS Biology 8 (12): e1000562. doi:10.1371/journal.pbio.1000562.
  • Ferdous, Z., and A. Nemmar. 2020. “Health Impact of Silver Nanoparticles: A Review of the Biodistribution and Toxicity following Various Routes of Exposure.” International Journal of Molecular Sciences 21 (7): 2375. doi:10.3390/ijms21072375.
  • Foldbjerg, R., D. A. Dang, and H. Autrup. 2011. “Cytotoxicity and Genotoxicity of Silver Nanoparticles in the Human Lung Cancer Cell Line, A549.” Archives of Toxicology 85 (7): 743–750. doi:10.1007/s00204-010-0545-5.
  • Gaiser, B. K., S. Hirn, A. Kermanizadeh, N. Kanase, K. Fytianos, A. Wenk, N. Haberl, A. Brunelli, W. G. Kreyling, and V. Stone. 2013. “Effects of Silver Nanoparticles on the Liver and Hepatocytes In Vitro.” Toxicological Sciences 131 (2): 537–547. doi:10.1093/toxsci/kfs306.
  • Gao, J., C. T. Mahapatra, C. D. Mapes, M. Khlebnikova, A. Wei, and M. S. Sepúlveda. 2016. “Vascular Toxicity of Silver Nanoparticles to Developing Zebrafish (Danio rerio).” Nanotoxicology 10 (9): 1363–1372. doi:10.1080/17435390.2016.1214763.
  • Goncalves, D. M., and D. Girard. 2014. “Zinc Oxide Nanoparticles Delay Human Neutrophil Apoptosis by a de Novo Protein Synthesis-Dependent and Reactive Oxygen Species-Independent Mechanism.” Toxicology In Vitro 28 (5): 926–931. doi:10.1016/j.tiv.2014.03.002.
  • Gratacap, R. L., J. F. Rawls, and R. T. Wheeler. 2013. “Mucosal Candidiasis Elicits NF-B Activation, Proinflammatory Gene Expression and Localized Neutrophilia in Zebrafish.” Disease Models & Mechanisms 6 (5): 1260–1270. doi:10.1242/dmm.012039.
  • Gray, C., C. Loynes, M. Whyte, D. Crossman, S. A. Renshaw, and T. Chico. 2011. “Simultaneous Intravital Imaging of Macrophage and Neutrophil Behaviour during Inflammation Using a Novel Transgenic Zebrafish.” Thrombosis and Haemostasis 105 (5): 811–819. doi:10.1160/TH10-08-0525.
  • Green, J. M., A. Lange, A. Scott, M. Trznadel, H. A. Wai, A. Takesono, A. R. Brown, S. F. Owen, T. Kudoh, and C. R. Tyler. 2018. “Early Life Exposure to Ethinylestradiol Enhances Subsequent Responses to Environmental Estrogens Measured in a Novel Transgenic Zebrafish.” Scientific Reports 8 (1): 2699. doi:10.1038/s41598-018-20922-z.
  • Hall, C. J., M. V. Flores, S. H. Oehlers, L. E. Sanderson, E. Y. Lam, K. E. Crosier, and P. S. Crosier. 2012. “Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish is Dependent upon Inducible Nitric Oxide.” Cell Stem Cell 10 (2): 198–209. doi:10.1016/j.stem.2012.01.007.
  • Harvie, E. A., and A. Huttenlocher. 2015. “Non-Invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection.” Journal of Visualized Experiments: JoVE (98): 52788. doi:10.3791/52788.
  • He, J.-H., J.-M. Gao, C.-J. Huang, and C.-Q. Li. 2014. “Zebrafish Models for Assessing Developmental and Reproductive Toxicity.” Neurotoxicology and Teratology 42: 35–42. doi:10.1016/j.ntt.2014.01.006.
  • Home Office. 1986. Animals (Scientific Procedures) Act 1986.
  • Hoodless, L. J., C. D. Lucas, R. Duffin, M. A. Denvir, C. Haslett, C. S. Tucker, and A. G. Rossi. 2016. “Genetic and Pharmacological Inhibition of CDK9 Drives Neutrophil Apoptosis to Resolve Inflammation in Zebrafish In Vivo.” Scientific Reports 6 (1): 36980. doi:10.1038/srep36980.
  • Hou, Y., Z. Sheng, X. Mao, C. Li, J. Chen, J. Zhang, H. Huang, H. Ruan, L. Luo, and L. Li. 2016. “Systemic Inoculation of Escherichia coli Causes Emergency Myelopoiesis in Zebrafish Larval Caudal Hematopoietic Tissue.” Scientific Reports 6: 36853. doi:10.1038/srep36853.
  • Howe, K., M. D. Clark, C. F. Torroja, J. Torrance, C. Berthelot, M. Muffato, J. E. Collins, et al. 2013. “The Zebrafish Reference Genome Sequence and Its Relationship to the Human Genome.” Nature 496 (7446): 498–503. doi:10.1038/nature12111.
  • Hua, J., M. G. Vijver, M. K. Richardson, F. Ahmad, and W. J. G. M. Peijnenburg. 2014. “Particle‐Specific Toxic Effects of Differently Shaped Zinc Oxide Nanoparticles to Zebrafish Embryos (Danio rerio).” Environmental Toxicology and Chemistry 33 (12): 2859–2868. doi:10.1002/etc.2758.
  • Jacobsen, N. R., P. Møller, K. A. Jensen, U. Vogel, O. Ladefoged, S. Loft, and H. Wallin. 2009. “Lung Inflammation and Genotoxicity following Pulmonary Exposure to Nanoparticles in ApoE-/- Mice.” Particle and Fibre Toxicology 6: 2. doi:10.1186/1743-8977-6-2.
  • Jim, K. K., J. Engelen-Lee, A. M. van der Sar, W. Bitter, M. C. Brouwer, A. van der Ende, J. W. Veening, D. van de Beek, and C. M. Vandenbroucke-Grauls. 2016. “Infection of Zebrafish Embryos with Live Fluorescent Streptococcus pneumoniae as a Real-Time Pneumococcal Meningitis Model.” Journal of Neuroinflammation 13 (1): 188. doi:10.1186/s12974-016-0655-y.
  • Johnston, H., D. M. Brown, N. Kanase, M. Euston, B. K. Gaiser, C. T. Robb, E. Dyrynda, A. G. Rossi, E. R. Brown, and V. Stone. 2015. “Mechanism of Neutrophil Activation and Toxicity Elicited by Engineered Nanomaterials.” Toxicology In Vitro 29 (5): 1172–1184. doi:10.1016/j.tiv.2015.04.021.
  • Johnston, H. J., R. Verdon, S. Gillies, D. M. Brown, T. F. Fernandes, T. B. Henry, A. G. Rossi, et al. 2018. “Adoption of In Vitro Systems and Zebrafish Embryos as Alternative Models for Reducing Rodent Use in Assessments of Immunological and Oxidative Stress Responses to Nanomaterials.” Critical Reviews in Toxicology 48 (3): 252–271. doi:10.1080/10408444.2017.1404965.
  • Kaveh, A., F. A. Bruton, C. Buckley, M. E. M. Oremek, C. S. Tucker, J. J. Mullins, J. M. Taylor, A. G. Rossi, and M. A. Denvir. 2020. “Live Imaging of Heart Injury in Larval Zebrafish Reveals a Multi-Stage Model of Neutrophil and Macrophage Migration.” Frontiers in Cell and Developmental Biology 8: 579943. doi:10.3389/fcell.2020.579943.
  • Keller, Johannes G., Michael Persson, Philipp Müller, Lan Ma-Hock, Kai Werle, Josje Arts, Robert Landsiedel, and Wendel Wohlleben. 2021. “Variation in Dissolution Behavior among Different Nanoforms and Its Implication for Grouping Approaches in Inhalation Toxicity.” Nanoimpact 23: 100341. doi:10.1016/j.impact.2021.100341.
  • Keller, J. G., W. Peijnenburg, K. Werle, R. Landsiedel, and W. Wohlleben. 2020. “Understanding Dissolution Rates via Continuous Flow Systems with Physiologically Relevant Metal Ion Saturation in Lysosome.” Nanomaterials 10 (2): 311. doi:10.3390/nano10020311.
  • Kermanizadeh, A., D. M. Brown, G. R. Hutchison, and V. Stone. 2012. “Engineered Nanomaterial Impact in the Liver following Exposure via an Intravenous Route–The Role of Polymorphonuclear Leukocytes and Gene Expression in the Organ.” Journal of Nanomedicine & Nanotechnology 4 (1). doi:10.4172/2157-7439.1000157.
  • Kermanizadeh, Ali, Giulio Pojana, Birgit K. Gaiser, Renie Birkedal, Dagmar Bilanicová, Håkan Wallin, Keld Alstrup Jensen, et al. 2013a. “In Vitro Assessment of Engineered Nanomaterials Using a Hepatocyte Cell Line: Cytotoxicity, Pro-Inflammatory Cytokines and Functional Markers.” Nanotoxicology 7 (3): 301–313. doi:10.3109/17435390.2011.653416.
  • Kermanizadeh, A., S. Vranic, S. Boland, K. Moreau, A. Baeza-Squiban, B. K. Gaiser, L. A. Andrzejczuk, and V. Stone. 2013b. “An In Vitro Assessment of Panel of Engineered Nanomaterials Using a Human Renal Cell Line: cytotoxicity, Pro-Inflammatory Response, Oxidative Stress and Genotoxicity.” BMC Nephrology 14: 96. doi:10.1186/1471-2369-14-96.
  • Kim, G. R., J. Y. Yang, K.-S. Hwang, S. S. Kim, J. S. Chae, H. Kan, J. H. Ahn, et al. 2019. “Anti-Inflammatory Effect of a Novel Synthetic Compound 1-((4-Fluorophenyl)Thio)Isoquinoline in RAW264.7 Macrophages and a Zebrafish Model.” Fish & Shellfish Immunology 87: 395–400. doi:10.1016/j.fsi.2019.01.030.
  • Kim, M. J., K. H. Kang, C. H. Kim, and S. Y. Choi. 2008. “Real-Time Imaging of Mitochondria in Transgenic Zebrafish Expressing Mitochondrially Targeted GFP.” BioTechniques 45 (3): 331–334. doi:10.2144/000112909.
  • Klein, C. L., S. Comero, B. Stahlmecke, J. Romazanov, T. A. J. Kuhlbusch, E. Van. Doren, P.-J. De, et al. 2011. NM-Series of Representative Manufactured Nanomaterials NM-300 Silver Characterisation, Stability, Homogeneity. doi:10.2788/23079.
  • Kovrižnych, J. A., R. Sotníková, D. Zeljenková, E. Rollerová, E. Szabová, and S. Wimmerová. 2013. “Acute Toxicity of 31 Different Nanoparticles to Zebrafish (Danio rerio) Tested in Adulthood and in Early Life Stages – Comparative Study.” Interdisciplinary Toxicology 6 (2): 67–73. doi:10.2478/intox-2013-0012.
  • Küçükoğlu, M., U. S. Bi̇Nokay, and A. B. Pekmezekmek. 2013. “The Effects of Zinc Chloride during Early Embryonic Development in Zebrafish.” Turkish Journal of Biology 37: 158–164.
  • Kusik, B. W., M. J. Carvan, 3rd, and A. J. Udvadia. 2008. “Detection of Mercury in Aquatic Environments Using EPRE Reporter Zebrafish.” Marine Biotechnology 10 (6): 750–757. doi:10.1007/s10126-008-9113-x.
  • Landsiedel, R., U. G. Sauer, L. Ma-Hock, J. Schnekenburger, and M. Wiemann. 2014. “Pulmonary Toxicity of Nanomaterials: A Critical Comparison of Published In Vitro Assays and In Vivo Inhalation or Instillation Studies.” Nanomedicine 9 (16): 2557–2585. doi:10.2217/nnm.14.149.
  • Lebedová, J., Y. S. Hedberg, I. Odnevall Wallinder, and H. L. Karlsson. 2018. “Size-Dependent Genotoxicity of Silver, Gold and Platinum Nanoparticles Studied Using the Mini-Gel Comet Assay and Micronucleus Scoring with Flow Cytometry.” Mutagenesis 33 (1): 77–85. doi:10.1093/mutage/gex027.
  • Lee, O., J. M. Green, and C. R. Tyler. 2015. “Transgenic Fish Systems and Their Application in Ecotoxicology.” Critical Reviews in Toxicology 45 (2): 124–141. doi:10.3109/10408444.2014.965805.
  • Lee, Okhyun, Aya Takesono, Masazumi Tada, Charles R. Tyler, and Tetsuhiro Kudoh. 2012. “Biosensor Zebrafish Identify Body Target Tissues for Environmental Estrogens.” Environmental Health Perspectives 120 (7): 990–996. doi:10.1289/ehp.1104433.
  • Levraud, J.-P., E. Colucci-Guyon, M. J. Redd, G. Lutfalla, and P. Herbomel. 2008. “In Vivo Analysis of Zebrafish Innate Immunity.” In Innate Immunity, Methods in Molecular BiologyTM, edited by J. Ewbank and E. Vivier, 337–363. Totowa, NJ: Humana Press. doi:10.1007/978-1-59745-570-1_20.
  • Li, Y., and B. Hu. 2012. “Establishment of Multi-Site Infection Model in Zebrafish Larvae for Studying Staphylococcus aureus Infectious Disease.” Journal of Genetics and Genomics 39 (9): 521–534. doi:10.1016/j.jgg.2012.07.006.
  • Liang, J., W. Jin, H. Li, H. Liu, Y. Huang, X. Shan, C. Li, L. Shan, and T. Efferth. 2016. “In Vivo Cardiotoxicity Induced by Sodium Aescinate in Zebrafish Larvae.” Molecules 21 (3): 190. doi:10.3390/molecules21030190.
  • Lieschke, G. J., and P. D. Currie. 2007. “Animal Models of Human Disease: zebrafish Swim into View.” Nature Reviews. Genetics 8 (5): 353–367. doi:10.1038/nrg2091.
  • Lieschke, G. J., and N. S. Trede. 2009. “Fish Immunology.” Current Biology 19 (16): R678–R682. doi:10.1016/j.cub.2009.06.068.
  • Loynes, C. A., J. S. Martin, A. Robertson, D. M. I. Trushell, P. W. Ingham, M. K. B. Whyte, and S. A. Renshaw. 2010. “Pivotal Advance: Pharmacological Manipulation of Inflammation Resolution during Spontaneously Resolving Tissue Neutrophilia in the Zebrafish.” Journal of Leukocyte Biology 87 (2): 203–212. doi:10.1189/jlb.0409255.
  • Lucas, C. D., K. C. Allen, D. A. Dorward, L. J. Hoodless, L. A. Melrose, J. A. Marwick, C. S. Tucker, C. Haslett, R. Duffin, and A. G. Rossi. 2013. “Flavones Induce Neutrophil Apoptosis by down-Regulation of Mcl-1 via a Proteasomal-Dependent Pathway.” FASEB Journal 27 (3): 1084–1094. doi:10.1096/fj.12-218990.
  • Massarsky, A., L. Dupuis, J. Taylor, S. Eisa-Beygi, L. Strek, V. L. Trudeau, and T. W. Moon. 2013. “Assessment of Nanosilver Toxicity during Zebrafish (Danio rerio) Development.” Chemosphere 92 (1): 59–66. doi:10.1016/j.chemosphere.2013.02.060.
  • Metzemaekers, M., M. Gouwy, and P. Proost. 2020. “Neutrophil Chemoattractant Receptors in Health and Disease: Double-Edged Swords.” Cellular & Molecular Immunology 17 (5): 433–418. doi:10.1038/s41423-020-0412-0.
  • Miskolci, V., J. Squirrell, J. Rindy, W. Vincent, J. D. Sauer, A. Gibson, K. W. Eliceiri, and A. Huttenlocher. 2019. “Distinct Inflammatory and Wound Healing Responses to Complex Caudal Fin Injuries of Larval Zebrafish.” eLife 8: e45976. doi:10.7554/eLife.45976.
  • Mottaz, H., R. Schönenberger, S. Fischer, R. I. L. Eggen, K. Schirmer, and K. J. Groh. 2017. “Dose-Dependent Effects of Morphine on Lipopolysaccharide (LPS)-Induced Inflammation, and Involvement of Multixenobiotic Resistance (MXR) Transporters in LPS Efflux in Teleost Fish.” Environmental Pollution 221: 105–115. doi:10.1016/j.envpol.2016.11.046.
  • Mourabit, S., J. A. Fitzgerald, R. P. Ellis, A. Takesono, C. S. Porteus, M. Trznadel, J. Metz, M. J. Winter, T. Kudoh, and C. R. Tyler. 2019. “New Insights into Organ-Specific Oxidative Stress Mechanisms Using a Novel Biosensor Zebrafish.” Environment International 133 (Pt A): 105138. doi:10.1016/j.envint.2019.105138.
  • Muth-Köhne, E., L. Sonnack, K. Schlich, F. Hischen, W. Baumgartner, K. Hund-Rinke, C. Schäfers, and M. Fenske. 2013. “The Toxicity of Silver Nanoparticles to Zebrafish Embryos Increases through Sewage Treatment Processes.” Ecotoxicology 22 (8): 1264–1277. doi:10.1007/s10646-013-1114-5.
  • Nguyen-Chi, M., B. Laplace-Builhé, J. Travnickova, P. Luz-Crawford, G. Tejedor, G. Lutfalla, K. Kissa, C. Jorgensen, and F. Djouad. 2017. “TNF Signaling and Macrophages Govern Fin Regeneration in Zebrafish Larvae.” Cell Death & Disease 8 (8): e2979. doi:10.1038/cddis.2017.374.
  • Nguyen-Chi, M., Q. T. Phan, C. Gonzalez, J. F. Dubremetz, J. P. Levraud, and G. Lutfalla. 2014. “Transient Infection of the Zebrafish Notochord with E. coli Induces Chronic Inflammation.” Disease Models & Mechanisms 7 (7): 871–882. doi:10.1242/dmm.014498.
  • Novoa, B, and A. Figueras. 2012. “Zebrafish: Model for the Study of Inflammation and the Innate Immune Response to Infectious Diseases.” In Current Topics in Innate Immunity II, Advances in Experimental Medicine and Biology, edited by J. D. Lambris and G. Hajishengallis, 253–275. New York, NY: Springer. doi:10.1007/978-1-4614-0106-3_15.
  • Odzak, N., D. Kistler, R. Behra, and L. Sigg. 2014. “Dissolution of Metal and Metal Oxide Nanoparticles in Aqueous Media.” Environmental Pollution 191: 132–138. doi:10.1016/j.envpol.2014.04.010.
  • Osborne, O. J., B. D. Johnston, J. Moger, M. Balousha, J. R. Lead, T. Kudoh, and C. R. Tyler. 2013. “Effects of Particle Size and Coating on Nanoscale Ag and TiO2 Exposure in Zebrafish (Danio rerio) Embryos.” Nanotoxicology 7 (8): 1315–1324. doi:10.3109/17435390.2012.737484.
  • Park, H. C., C. H. Kim, Y. K. Bae, S. Y. Yeo, S. H. Kim, S. K. Hong, J. Shin, et al. 2000. “Analysis of Upstream Elements in the HuC Promoter Leads to the Establishment of Transgenic Zebrafish with Fluorescent Neurons.” Developmental Biology 227 (2): 279–293. doi:10.1006/dbio.2000.9898.
  • Patlolla, A. K., D. Hackett, and P. B. Tchounwou. 2015. “Silver Nanoparticle-Induced Oxidative Stress-Dependent Toxicity in Sprague-Dawley Rats.” Molecular and Cellular Biochemistry 399 (1–2): 257–268. doi:10.1007/s11010-014-2252-7.
  • Peijnenburg, W. J. G. M., E. Ruggiero, M. Boyles, F. Murphy, V. Stone, D. A. Elam, K. Werle, and W. Wohlleben. 2020. “A Method to Assess the Relevance of Nanomaterial Dissolution during Reactivity Testing.” Materials 13 (10): 2235. doi:10.3390/ma13102235.
  • Philip, A. M., Y. Wang, A. Mauro, S. El-Rass, J. C. Marshall, W. L. Lee, A. S. Slutsky, C. C. dos Santos, and X.-Y. Wen. 2017. “Development of a Zebrafish Sepsis Model for High-Throughput Drug Discovery.” Molecular Medicine 23: 134–148. doi:10.2119/molmed.2016.00188.
  • Powell, D., S. Tauzin, L. E. Hind, Q. Deng, D. J. Beebe, and A. Huttenlocher. 2017. “Chemokine Signaling and the Regulation of Bidirectional Leukocyte Migration in Interstitial Tissues.” Cell Reports 19 (8): 1572–1585. doi:10.1016/j.celrep.2017.04.078.
  • Rawls, J. F., E. M. Mellgren, and S. L. Johnson. 2001. “How the Zebrafish Gets Its Stripes.” Developmental Biology 240 (2): 301–314. doi:10.1006/dbio.2001.0418.
  • Ren, D.-L., Y. Li, Bing-bing. Hu, H. Wang, and Bing. Hu. 2015a. “Melatonin Regulates the Rhythmic Migration of Neutrophils in Live Zebrafish.” Journal of Pineal Research 58 (4): 452–460. doi:10.1111/jpi.12230.
  • Ren, D.-L., A.-A. Sun, Y.-J. Li, M. Chen, S.-C. Ge, and B. Hu. 2015b. “Exogenous Melatonin Inhibits Neutrophil Migration through Suppression of ERK Activation.” The Journal of Endocrinology 227 (1): 49–60. doi:10.1530/JOE-15-0329.
  • Renshaw, S. A., C. A. Loynes, S. Elworthy, P. W. Ingham, and M. K. Whyte. 2007. “Modeling Inflammation in the Zebrafish: How a Fish Can Help us Understand Lung Disease.” Experimental Lung Research 33 (10): 549–554. doi:10.1080/01902140701756778.
  • Renshaw, S. A., C. A. Loynes, D. M. I. Trushell, S. Elworthy, P. W. Ingham, and M. K. B. Whyte. 2006. “A Transgenic Zebrafish Model of Neutrophilic Inflammation.” Blood 108 (13): 3976–3978. doi:10.1182/blood-2006-05-024075.
  • Research and Markets. 2018. Global Nanotechnology Market (by Component and Applications), Funding & Investment, Patent Analysis and 27 Companies Profile & Recent Developments – Forecast to 2024. Report ID: 4520812.
  • Schneider, C. A., W. S. Rasband, and K. W. Eliceiri. 2012. “NIH Image to ImageJ: 25 Years of Image Analysis.” Nature Methods 9 (7): 671–675. doi:10.1038/nmeth.2089.
  • Sha, B., W. Gao, S. Wang, X. Gou, W. Li, X. Liang, Z. Qu, F. Xu, and T. J. Lu. 2014. “Oxidative Stress Increased Hepatotoxicity Induced by Nano-Titanium Dioxide in BRL-3A Cells and Sprague-Dawley Rats: Oxidative Stress Increased Hepatotoxicity Induced by nano-TiO2.” Journal of Applied Toxicology 34 (4): 345–356. doi:10.1002/jat.2900.
  • Sharif, Faiza, Fabiola Porta, Annemarie H. Meijer, Alexander Kros, and Michael K. Richardson. 2012. “Mesoporous Silica Nanoparticles as a Compound Delivery System in Zebrafish Embryos.” International Journal of Nanomedicine 7: 1875–1890. doi:10.2147/IJN.S26547.
  • Singh, C., S. Friedrichs, M. Levin, R. Birkedal, K. A. Jensen, G. Pojana, W. Wohlleben, et al. 2011. NM-Series of Representative Manufactured Nanomaterials – Zinc Oxide NM-110, NM-111, NM-112, NM-113: Characterisation and Test Item Preparation (EUR – Scientific and Technical Research Reports). Publications Office of the European Union. doi:10.2787/55008.
  • Smulders, S., C. Larue, G. Sarret, H. Castillo-Michel, J. Vanoirbeek, and P. H. M. Hoet. 2015. “Lung Distribution, Quantification, co-Localization and Speciation of Silver Nanoparticles after Lung Exposure in Mice.” Toxicology Letters 238 (1): 1–6. doi:10.1016/j.toxlet.2015.07.001.
  • Smulders, S., K. Luyts, G. Brabants, K. V. Landuyt, C. Kirschhock, E. Smolders, L. Golanski, J. Vanoirbeek, and P. H. Hoet. 2014. “Toxicity of Nanoparticles Embedded in Paints Compared with Pristine Nanoparticles in Mice.” Toxicological Sciences 141 (1): 132–140. doi:10.1093/toxsci/kfu112.
  • Stone, V., H. Johnston, and M. J. D. Clift. 2007. “Air Pollution, Ultrafine and Nanoparticle Toxicology: Cellular and Molecular Interactions.” IEEE Transactions on Nanobioscience 6 (4): 331–340. doi:10.1109/tnb.2007.909005.
  • Subramanian, B. C., K. Moissoglu, and C. A. Parent. 2018. “The LTB 4 –BLT1 Axis Regulates the Polarized Trafficking of Chemoattractant GPCRs during Neutrophil Chemotaxis.” Journal of Cell Science 131: jcs217422. doi:10.1242/jcs.217422.
  • Tavares, A. M., H. Louro, S. Antunes, S. Quarré, S. Simar, P. J. De Temmerman, E. Verleysen, et al. 2014. “Genotoxicity Evaluation of Nanosized Titanium Dioxide, Synthetic Amorphous Silica and Multi-Walled Carbon Nanotubes in Human Lymphocytes.” Toxicology In Vitro 28 (1): 60–69. doi:10.1016/j.tiv.2013.06.009.
  • Torraca, V., C. Cui, R. Boland, J.-P. Bebelman, A. M. van der Sar, M. J. Smit, M. Siderius, H. P. Spaink, and, A. H. Meijer. 2015. “The CXCR3-CXCL11 Signaling Axis Mediates Macrophage Recruitment and Dissemination of Mycobacterial Infection.” Disease Models & Mechanisms 8 (3): 253–269. doi:10.1242/dmm.017756.
  • Torraca, V., N. A. Otto, A. Tavakoli-Tameh, and A. H. Meijer. 2017. “The Inflammatory Chemokine Cxcl18b Exerts Neutrophil-Specific Chemotaxis via the Promiscuous Chemokine Receptor Cxcr2 in Zebrafish.” Developmental and Comparative Immunology 67: 57–65. doi:10.1016/j.dci.2016.10.014.
  • Tortella, G. R., O. Rubilar, N. Durán, M. C. Diez, M. Martínez, J. Parada, and A. B. Seabra. 2020. “Silver Nanoparticles: Toxicity in Model Organisms as an Overview of Its Hazard for Human Health and the Environment.” Journal of Hazardous Materials 390: 121974. doi:10.1016/j.jhazmat.2019.121974.
  • van Aerle, R., A. Lange, A. Moorhouse, K. Paszkiewicz, K. Ball, B. D. Johnston, E. de-Bastos, T. Booth, C. R. Tyler, and E. M. Santos. 2013. “Molecular Mechanisms of Toxicity of Silver Nanoparticles in Zebrafish Embryos.” Environmental Science & Technology 47 (14): 8005–8014. doi:10.1021/es401758d.
  • Vance, Marina E., Todd Kuiken, Eric P. Vejerano, Sean P. McGinnis, Michael F. Hochella, David Rejeski, and Matthew S. Hull. 2015. “Nanotechnology in the Real World: Redeveloping the Nanomaterial Consumer Products Inventory.” Beilstein Journal of Nanotechnology 6: 1769–1780. doi:10.3762/bjnano.6.181.
  • Vandebriel, R. J., and W. H. De Jong. 2012. “A Review of Mammalian Toxicity of ZnO Nanoparticles.” Nanotechnology, Science and Applications 5: 61–71. doi:10.2147/NSA.S23932.
  • Varas, M., A. Fariña, F. Díaz-Pascual, J. Ortíz-Severín, A. E. Marcoleta, M. L. Allende, C. A. Santiviago, and F. P. Chávez. 2017. “Live-Cell Imaging of Salmonella typhimurium Interaction with Zebrafish Larvae after Injection and Immersion Delivery Methods.” Journal of Microbiological Methods 135: 20–25. doi:10.1016/j.mimet.2017.01.020.
  • Verdon, R., S. L. Gillies, D. M. Brown, T. Henry, L. Tran, C. R. Tyler, A. G. Rossi, V. Stone, and H. J. Johnston. 2021. “Neutrophil Activation by Nanomaterials In Vitro: Comparing Strengths and Limitations of Primary Human Cells with Those of an Immortalized (HL-60) Cell Line.” Nanotoxicology 15 (1): 1–20. doi:10.1080/17435390.2020.1834635.
  • Vincent, W. J. B., E. A. Harvie, J.-D. Sauer, and A. Huttenlocher. 2017. “Neutrophil Derived LTB4 Induces Macrophage Aggregation in Response to Encapsulated Streptococcus iniae Infection.” PLOS One 12 (6): e0179574. doi:10.1371/journal.pone.0179574.
  • Wehmas, L. C., C. Anders, J. Chess, A. Punnoose, C. B. Pereira, J. A. Greenwood, and R. L. Tanguay. 2015. “Comparative Metal Oxide Nanoparticle Toxicity Using Embryonic Zebrafish.” Toxicology Reports 2: 702–715. doi:10.1016/j.toxrep.2015.03.015.
  • Westerfield, M. 2000. The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio rerio), 4th ed. Eugene, OR: University of Oregon Press.
  • Winter, Matthew J., Joseph Pinion, Anna Tochwin, Aya Takesono, Jonathan S. Ball, Piotr Grabowski, Jeremy Metz, et al. 2021. “Functional Imaging in Larval Zebrafish for Characterising the Effects of Proconvulsant Compounds Acting via a Range of Pharmacological Mechanisms.” British Journal of Pharmacology 178 (13): 2671–2689. doi:10.1111/bph.15458.
  • Yang, C.-T., C. J. Cambier, J. M. Davis, C. J. Hall, P. S. Crosier, and L. Ramakrishnan. 2012. “Neutrophils Exert Protection in the Early Tuberculous Granuloma by Oxidative Killing of Mycobacteria Phagocytosed from Infected Macrophages.” Cell Host & Microbe 12 (3): 301–312. doi:10.1016/j.chom.2012.07.009.
  • Yang, L., X. Zhou, W. Huang, Q. Fang, J. Hu, L. Yu, N. Ma, and W. Zhang. 2017. “Protective Effect of Phillyrin on Lethal LPS-Induced Neutrophil Inflammation in Zebrafish.” Cellular Physiology and Biochemistry 43 (5): 2074–2087. doi:10.1159/000484192.
  • Yang, L.-L., G.-Q. Wang, L.-M. Yang, Z.-B. Huang, W.-Q. Zhang, and L.-Z. Yu. 2014. “Endotoxin Molecule Lipopolysaccharide-Induced Zebrafish Inflammation Model: A Novel Screening Method for Anti-Inflammatory Drugs.” Molecules 19 (2): 2390–2409. doi:10.3390/molecules19022390.
  • Yoo, S. K., T. W. Starnes, Q. Deng, and A. Huttenlocher. 2011. “Lyn is a Redox Sensor That Mediates Leukocyte Wound Attraction In Vivo.” Nature 480 (7375): 109–112. doi:10.1038/nature10632.
  • Zhang, X., C. Li, and Z. Gong. 2014. “Development of a Convenient In Vivo Hepatotoxin Assay Using a Transgenic Zebrafish Line with Liver-Specific DsRed Expression.” PLOS One 9 (3): e91874. doi:10.1371/journal.pone.0091874.
  • Zhang, Y., H. Liu, J. Yao, Y. Huang, S. Qin, Z. Sun, Y. Xu, et al. 2016. “Manipulating the Air-Filled Zebrafish Swim Bladder as a Neutrophilic Inflammation Model for Acute Lung Injury.” Cell Death & Disease 7 (11): e2470. doi:10.1038/cddis.2016.365.
  • Zhu, X., L. Zhu, Z. Duan, R. Qi, Y. Li, and Y. Lang. 2008. “Comparative Toxicity of Several Metal Oxide Nanoparticle Aqueous Suspensions to Zebrafish (Danio rerio) Early Developmental Stage.” Journal of Environmental Science and Health. Part A, Toxic/Hazardous Substances & Environmental Engineering 43 (3): 278–284. doi:10.1080/10934520701792779.