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Articles

Denaturing gradient gel electrophoresis (DGGE)-based monitoring of intestinal lactobacilli and bifidobacteria of pigs during a feeding trial

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Pages 112-126 | Received 13 Oct 2008, Accepted 22 Dec 2008, Published online: 06 Apr 2009

References

  • Adjiri-Awere , A and Van Lunen , T A . 2005 . Subtherapeutic use of antibiotics in pork production: Risks and alternatives . Can J Anim Sci , 85 : 117 – 130 .
  • Baker , R . 2006 . Health management with reduced antibiotic use – the U.S. experience . Anim Biotechnol , 17 : 195 – 205 .
  • Brunsgaard , G . 1998 . Effects of cereal type and feed particle size on morphological characteristics, epithelial cell proliferation, and lectin binding patterns in the large intestine of pigs . J Anim Sci , 76 : 2787 – 2798 .
  • Casey , P G , Gardiner , G E , Casey , G , Bradshaw , B , Lawlor , P G , Lynch , P B , Leonard , F C , Stanton , C , Ross , R P , Fitzgerald , G F and Hill , C . 2007 . A five-strain probiotic combination reduces pathogen shedding and alleviates disease signs in pigs challenged with Salmonella enterica serovar typhimurium . Appl Environ Microbiol , 73 ( 6 ) : 1858 – 1863 .
  • Council of the European Union . 1999 . Council Regulation (EC) No 1804/1999 of 19 July 1999 supplementing Regulation (EEC) No 2092/91 on organic production of agricultural products and indications referring thereto on agricultural products and foodstuffs to include livestock production . Official Journal L , 222 : 0001 – 0028 .
  • Gavini , F , Delcenserie , V , Kopeinig , K , Pollinger , S , Beerens , H , Bonaparte , C and Upmann , M . 2006 . Bifidobacterium species isolated from animal feces and from beef and pork meat . J Food Prot , 69 : 871 – 877 .
  • Gong , J , Yu , H , Liu , T , Gill , J J , Chambers , J R , Wheatcroft , R and Sabour , P M . 2008a . Effects of zinc bacitracin, bird age and access to range on bacterial microbiota in the ileum and caeca of broiler chickens . J Appl Microbiol , 104 : 1372 – 1382 .
  • Gong , J , Yu , H , Liu , T , Li , M , Si , W , De Lange , C FM and Dewey , C . 2008b . Characterization of ileal bacterial microbiota in newly-weaned pigs in response to feeding lincomycin, organic acids or herbal extract . Livest Sci , 116 : 318 – 322 .
  • Janczyk , P , Pieper , R , Smidt , H and Souffrant , W B . 2007 . Changes in the diversity of pig ileal lactobacilli around weaning determined by means of 16S rRNA gene amplification and denaturing gradient gel electrophoresis . FEMS Microbiol Ecol , 61 : 132 – 140 .
  • Knarreborg , A , Simon , M A , Engberg , R M , Jensen , B B and Tannock , G W . 2002 . Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages . Appl Environ Microbiol , 68 : 5918 – 5924 .
  • Konstantinov , S R , Favier , C F , Zhu , W Y , Williams , B A , Klüß , J , Souffrant , W B , De Vos , W M , Akkermans , A DL and Smidt , H . 2004 . Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition . Anim Res , 53 : 317 – 324 .
  • Li , M , Gong , J , Cottrill , M , Yu , H , De Lange , C , Burton , J and Topp , E . 2003 . Evaluation of QIAamp® DNA Stool Mini Kit for ecological studies of gut microbiota . J Microbiol Methods , 54 : 13 – 20 .
  • Maukonen , J , Mättö , J , Kajander , K , Mattila-Sandholm , T and Saarela , M . 2008 . Diversity and temporal stability of fecal bacterial populations in elderly subjects consuming galacto-oligosaccharide containing probiotic yoghurt . Int Dairy J , 18 : 386 – 395 .
  • McCartney , A L . 2002 . Applications of molecular biological methods for studying probiotics and the gut flora . Brit J Nutr , 88 : 29 – 37 .
  • Muyzer , G and Smalla , K . 1998 . Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology . Antonie van Leeuwenhoek , 73 : 127 – 141 .
  • Nagel , P , Domig , K J , Hagmüller , W , Pfalz , S , Kronsteiner , S , Ortner , B , Sundrum , A and Zollitsch , W . Effects of silage or probiotics on performance and gut microbial composition of organic growing-finishing pigs. 6 . BOKU-Symposium Tierernährung, Sekundärwirkungen von Futterinhaltsstoffen – vom Nährstoff zum Wirkstoff . November 15 2007 , Vienna , Austria.
  • Namsolleck , P , Thiel , R , Lawson , P , Holmstrøm , K , Rajilic , M , Vaughan , E E , Rigottier-Gois , L , Collins , M D , De Vos , W M and Blaut , M . 2004 . Molecular methods for the analysis of gut microbiota . Microb Ecol Health Dis , 16 : 71 – 85 .
  • O'Sullivan , D J . 2000 . Methods for analysis of the intestinal microflora. Curr Issues Intest . Microbiol , 1 ( 2 ) : 39 – 50 .
  • Pluske , J R , Durmic , Z , Payne , H G , Mansfield , J , Mullan , B P , Hampson , D J and Vercoe , P E . 2007 . Microbial diversity in the large intestine of pigs born and reared in different environments . Livest Sci , 108 : 113 – 116 .
  • Richards , J D , Gong , J and De Lange , C FM . 2005 . The gastrointestinal microbiota and its role in monogastric nutrition and health with an emphasis on pigs: Current understanding, possible modulations, and new technologies for ecological studies . Can J Anim Sci , 85 : 421 – 435 .
  • Richter , T , Padel , S , Lowman , S and Jansen , B . 2006 . “ The European market for organic food 2004/2005 ” . In The world of organic agriculture. Statistics and emerging trends 2006 , Edited by: Willer , H and Yussefi , M . 135 – 145 . Frick , , Switzerland : International Federation of Organic Agriculture Movements (IFOAM), Bonn Germany & Research Institute of Organic Agriculture FiBL .
  • Sanz , Y , Sánchez , E , Marzotto , M , Calabuig , M , Torriani , S and Dellaglio , F . 2007 . Differences in faecal bacterial communities in coeliac and healthy children as detected by PCR and denaturing gradient gel electrophoresis . FEMS Immunol Med Microbiol , 51 : 562 – 568 .
  • SAS Institute Inc . 2007 . SAS 9.1.3 Help and Documentation , 2000 – 2004 . Cary, NC , , USA : SAS Institute Inc .
  • Satokari , R M , Vaughan , E E , Akkermans , A DL , Saarela , M and De Vos , W H . 2001 . Bifidobacterial diversity in human feces detected by genus-specific PCR and Denaturing Gradient Gel Electrophoresis . Appl Environ Microbiol , 67 : 504 – 513 .
  • Scanlan , P D , Shanahan , F , O'Mahony , C and Marchesi , J R . 2006 . Culture-independent analyses of temporal variation of the dominant fecal microbiota and targeted bacterial subgroups in Crohn's disease . J Clin Microbiol , 44 : 3980 – 3988 .
  • Simon , O , Jadamus , A and Vahjen , W . 2001 . Probiotic feed additives – effectiveness and expected modes of action . J Anim Feed Sci , 10 : 51 – 67 .
  • Simpson , J M , McCracken , V J , White , B A , Gaskins , H R and Mackie , R I . 1999 . Application of denaturant gradient gel electrophoresis for the analysis of the porcine gastrointestinal microbiota . J Microbiol Methods , 36 : 167 – 179 .
  • Simpson , J M , McCracken , V J , Gaskins , H R and Mackie , R I . 2000 . Denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA amplicons to monitor changes in fecal bacterial populations of weaning pigs after introduction of Lactobacillus reuteri, strain MM53 . Appl Environ Microbiol , 66 : 4705 – 4714 .
  • Stege , H , Jensen , T K , Moller , K , Baekbo , P and Jorsal , S E . 2001 . Risk factors for intestinal pathogens in Danish finishing pig herds . Prevent Vet Med , 50 : 153 – 164 .
  • Tannock , G W . 1999 . Analysis of the intestinal microflora: a renaissance . Antonie van Leeuwenhoek , 76 : 265 – 278 .
  • Taras , D , Vahjen , W and Simon , O . 2007 . Probiotics in pigs – modulation of their intestinal distribution and their impact on health and performance . Livest Sci , 108 : 229 – 231 .
  • Vaughan , E E , De Vries , M C , Zoetendal , E G , Ben-Amor , K , Akkermans , A DL and De Vos , W H . 2002 . The intestinal LABs . Antonie van Leeuwenhoek , 82 : 341 – 352 .
  • Walter , J , Hertel , C , Tannock , G W , Lis , C M , Munro , K and Hammes , W . 2001 . Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and Denaturing Gradient Gel Electrophoresis . Appl Environ Microbiol , 67 : 2578 – 2585 .
  • Wang , H F , Zhu , W Y , Yao , W and Liu , J X . 2007 . DGGE and 16S rDNA sequencing analysis of bacterial communities in colon content and feces of pigs fed whole crop rice . Anaerobe , 13 : 127 – 133 .
  • Zentek , J . Diverse Kohlenhydrate in der Diätetik bei Durchfallerkrankungen der Absatzferkel. Paper presented at: “Aktuelle Probleme der Schweinefütterung”. Univeristy of Veterinary Medicine . Hanover , Germany.
  • Zhou , H , Gong , J , Brisbin , J T , Yu , H , Sanei , B , Sabour , P and Sharif , S . 2007 . Appropriate chicken sample size for identifying the composition of broiler intestinal microbiota affected by dietary antibiotics, using the polymerase chain reaction-denaturing gradient gel electrophoresis technique . Poult Sci , 86 : 2541 – 2549 .

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